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The map label for this gene is metF [H]

Identifier: 116688298

GI number: 116688298

Start: 315865

End: 316695

Strand: Direct

Name: metF [H]

Synonym: Bcen2424_0274

Alternate gene names: 116688298

Gene position: 315865-316695 (Clockwise)

Preceding gene: 116688297

Following gene: 116688301

Centisome position: 9.07

GC content: 66.67

Gene sequence:

>831_bases
ATGAAACCGATCGAACTCTCGTTTGAATTCTTCCCGCCGAAGACGGCGGACGGCGTCGAAAAGCTGCGCGCCACGCGCGC
GCAGCTGCTGCCGCTGAAGCCGAAATTCGTCTCCGTGACGTTCGGCGCCGGCGGCTCGACGCAGCAGGGCACGCTCGATA
CCGTGCTCGACATGCAGAAGGACGGCCTCGAGGCCGCGCCGCACCTGTCGTGCATCGGCTCGTCGCGCGACAGCCTGCGC
GCGATCCTCGACCAGTACCGCTCGCACGGCATCCGGCACATCGTCGCGCTGCGCGGCGACCTGCCGTCGGGGATGGGCGA
GGTCGGCGAGCTGCGCTATGCGTCCGAGCTCGTCAGCTTCATCCGCGCCGAGCATGGCGACTGGTTCCACATCGAAGTCG
CCGGTTATCCGGAGTACCACCCGCAATCGCGCTCGCCGAAGGCCGATCTCGAGAATTTCGCGCGCAAGGTGAAAGCCGGC
GCGAATTCCGCGATCACGCAGTACTTCTTCAACGCGGACGCGTATTTCCGCTTCGTCGACGATGCGCGCAAGCTGGGCGT
GGACGTGCCGATCGTGCCGGGCATCATGCCGATCACGAACTTCTCGCAGCTGATGCGCTTCTCCGAGATGTGCGGCGCCG
AAGTGCCGCGCTGGGTCGCGCGCCGGCTCGAGAGCTTCGGCGACGATCGCGAGTCGATCCGCGCGTTCGGCGCGGACGTC
GTCACGAACCTGTGCCAGCGCCTGATCGACGCGGGCGTGCCGGGGCTGCATTTCTACACGCTGAACTTGGCGACCGCCAC
GCGGACGATCTGCGAACGGCTCGCGGTGTAA

Upstream 100 bases:

>100_bases
ATCCTGTACAGCATTGTGTCGTGGCTGCTGTCCGCCCTGATCTTCGGCCAGCGCGACATCGGCTGAGAGCTGGCGGGCGC
AGGCCCAACCCCATCGAATC

Downstream 100 bases:

>100_bases
CGAGCGCCGTCACGCGGTTGCATGCGTGAAAGCCCCGCCGGTTTGCGCCGGCGGGGCTTTTTTATCGTGAAGCGCCGCGC
ATGCCGCGTGGCGGAAGCCG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKPIELSFEFFPPKTADGVEKLRATRAQLLPLKPKFVSVTFGAGGSTQQGTLDTVLDMQKDGLEAAPHLSCIGSSRDSLR
AILDQYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSFIRAEHGDWFHIEVAGYPEYHPQSRSPKADLENFARKVKAG
ANSAITQYFFNADAYFRFVDDARKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDRESIRAFGADV
VTNLCQRLIDAGVPGLHFYTLNLATATRTICERLAV

Sequences:

>Translated_276_residues
MKPIELSFEFFPPKTADGVEKLRATRAQLLPLKPKFVSVTFGAGGSTQQGTLDTVLDMQKDGLEAAPHLSCIGSSRDSLR
AILDQYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSFIRAEHGDWFHIEVAGYPEYHPQSRSPKADLENFARKVKAG
ANSAITQYFFNADAYFRFVDDARKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDRESIRAFGADV
VTNLCQRLIDAGVPGLHFYTLNLATATRTICERLAV
>Mature_276_residues
MKPIELSFEFFPPKTADGVEKLRATRAQLLPLKPKFVSVTFGAGGSTQQGTLDTVLDMQKDGLEAAPHLSCIGSSRDSLR
AILDQYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSFIRAEHGDWFHIEVAGYPEYHPQSRSPKADLENFARKVKAG
ANSAITQYFFNADAYFRFVDDARKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDRESIRAFGADV
VTNLCQRLIDAGVPGLHFYTLNLATATRTICERLAV

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=280, Percent_Identity=38.5714285714286, Blast_Score=189, Evalue=3e-48,
Organism=Escherichia coli, GI1790377, Length=274, Percent_Identity=46.3503649635037, Blast_Score=247, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI25147441, Length=282, Percent_Identity=33.6879432624113, Blast_Score=174, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI25147436, Length=282, Percent_Identity=33.6879432624113, Blast_Score=173, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6321313, Length=284, Percent_Identity=36.6197183098592, Blast_Score=178, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6325234, Length=289, Percent_Identity=32.8719723183391, Blast_Score=155, Evalue=5e-39,
Organism=Drosophila melanogaster, GI24662658, Length=270, Percent_Identity=26.6666666666667, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 30582; Mature: 30582

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIELSFEFFPPKTADGVEKLRATRAQLLPLKPKFVSVTFGAGGSTQQGTLDTVLDMQK
CCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHH
DGLEAAPHLSCIGSSRDSLRAILDQYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSF
CCCCCCCCHHHHCCCHHHHHHHHHHHHHCCHHEEEEECCCCCCCCHHHHHHHHHHHHHHH
IRAEHGDWFHIEVAGYPEYHPQSRSPKADLENFARKVKAGANSAITQYFFNADAYFRFVD
HHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHH
DARKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDRESIRAFGADV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
VTNLCQRLIDAGVPGLHFYTLNLATATRTICERLAV
HHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPIELSFEFFPPKTADGVEKLRATRAQLLPLKPKFVSVTFGAGGSTQQGTLDTVLDMQK
CCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHH
DGLEAAPHLSCIGSSRDSLRAILDQYRSHGIRHIVALRGDLPSGMGEVGELRYASELVSF
CCCCCCCCHHHHCCCHHHHHHHHHHHHHCCHHEEEEECCCCCCCCHHHHHHHHHHHHHHH
IRAEHGDWFHIEVAGYPEYHPQSRSPKADLENFARKVKAGANSAITQYFFNADAYFRFVD
HHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHH
DARKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWVARRLESFGDDRESIRAFGADV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHH
VTNLCQRLIDAGVPGLHFYTLNLATATRTICERLAV
HHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]