Click here to switch to the map view.

The map label for this gene is yaaI [H]

Identifier: 116625962

GI number: 116625962

Start: 8691540

End: 8692151

Strand: Reverse

Name: yaaI [H]

Synonym: Acid_6920

Alternate gene names: 116625962

Gene position: 8692151-8691540 (Counterclockwise)

Preceding gene: 116625963

Following gene: 116625961

Centisome position: 87.22

GC content: 58.5

Gene sequence:

>612_bases
ATGCGCGACATCAAAACTCGTCATACATCCTCCGTCGCTCTGGTCTTGATCGACGTCATCAACCACTTCGAATTCCCTGA
TGGCGACAAGATTCTCAAGCAGGCGCTACCGATGGCCGCCCGTCTGGCGAGGCTGAAGCAACGATGCCGAAAGGCTGGAA
TTCCGGCCATCTACGTCAACGACAATTTCGGCCAATGGCGGTCCGACGCAAAGAGCCTGATCGCGCGCTGCCTGGATTCA
AGCTGCGCCGGAAAGCCGTTCGTCGAGCAACTAAAGCCTGATGACAAGGATTACCTGGTGCTCAAGCCGATGCATTCGGC
TTTTTTCCAGACGCCTCTTGAAATCCTGCTGCAATACCTGGGCGCGACATCCCTGATCCTCACTGGCCTTGCGACAAACA
GCTGCATCGTGTGCACGGCGCATGACGCGAACATGCGGGATTTCATTCTGTACGTGCCCTCGGATTGTTCGGCGGCGCGC
AGCCGGCGCGAGCACACGCAGGCGATCGAGCACCTCAAAGAGACGACGCGCGCATCGGTCATTCCATCGGCCGGGTTGCG
AGTGGACGCGTTACGAGCCGCAAGCCGGCGCAATAGCAGAACCAAGGCTTAG

Upstream 100 bases:

>100_bases
ACCTCGTCTCCACCGCCGCTGCGGATGAAGGCGTGCCGGTTCAAAGGCGCGAGTACTCAGGCTGCCGTCATGACCTAGCT
GCTGGAGCAAGGCTGCTAAG

Downstream 100 bases:

>100_bases
CGTATGGGTACCCGCGATGCTAAACTTTCCGCCGCCGCGCCATCGTCCGCGCTCAGCAACGCGCAGATTGCCGACAAGTT
GGCTGGCCTCGCACAGTTAC

Product: isochorismatase hydrolase

Products: 2,3-Dihydro-2,3-dihydroxybenzoate; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MRDIKTRHTSSVALVLIDVINHFEFPDGDKILKQALPMAARLARLKQRCRKAGIPAIYVNDNFGQWRSDAKSLIARCLDS
SCAGKPFVEQLKPDDKDYLVLKPMHSAFFQTPLEILLQYLGATSLILTGLATNSCIVCTAHDANMRDFILYVPSDCSAAR
SRREHTQAIEHLKETTRASVIPSAGLRVDALRAASRRNSRTKA

Sequences:

>Translated_203_residues
MRDIKTRHTSSVALVLIDVINHFEFPDGDKILKQALPMAARLARLKQRCRKAGIPAIYVNDNFGQWRSDAKSLIARCLDS
SCAGKPFVEQLKPDDKDYLVLKPMHSAFFQTPLEILLQYLGATSLILTGLATNSCIVCTAHDANMRDFILYVPSDCSAAR
SRREHTQAIEHLKETTRASVIPSAGLRVDALRAASRRNSRTKA
>Mature_203_residues
MRDIKTRHTSSVALVLIDVINHFEFPDGDKILKQALPMAARLARLKQRCRKAGIPAIYVNDNFGQWRSDAKSLIARCLDS
SCAGKPFVEQLKPDDKDYLVLKPMHSAFFQTPLEILLQYLGATSLILTGLATNSCIVCTAHDANMRDFILYVPSDCSAAR
SRREHTQAIEHLKETTRASVIPSAGLRVDALRAASRRNSRTKA

Specific function: Required For Production Of 2,3-Dhb. Also Serves As An Aryl Carrier Protein And Plays A Role In Enterobactin Assembly. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

Organism=Escherichia coli, GI1786811, Length=165, Percent_Identity=29.0909090909091, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.3.2.1 [C]

Molecular weight: Translated: 22607; Mature: 22607

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDIKTRHTSSVALVLIDVINHFEFPDGDKILKQALPMAARLARLKQRCRKAGIPAIYVN
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
DNFGQWRSDAKSLIARCLDSSCAGKPFVEQLKPDDKDYLVLKPMHSAFFQTPLEILLQYL
CCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHH
GATSLILTGLATNSCIVCTAHDANMRDFILYVPSDCSAARSRREHTQAIEHLKETTRASV
CHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHC
IPSAGLRVDALRAASRRNSRTKA
CCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRDIKTRHTSSVALVLIDVINHFEFPDGDKILKQALPMAARLARLKQRCRKAGIPAIYVN
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
DNFGQWRSDAKSLIARCLDSSCAGKPFVEQLKPDDKDYLVLKPMHSAFFQTPLEILLQYL
CCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHH
GATSLILTGLATNSCIVCTAHDANMRDFILYVPSDCSAARSRREHTQAIEHLKETTRASV
CHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHC
IPSAGLRVDALRAASRRNSRTKA
CCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; Isochorismate [C]

Specific reaction: H2O + Isochorismate --> 2,3-Dihydro-2,3-dihydroxybenzoate + Pyruvate [C]

General reaction: Ether hydrolysis [C]

Inhibitor: Fe2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]