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The map label for this gene is ribH

Identifier: 116625278

GI number: 116625278

Start: 7802040

End: 7802516

Strand: Reverse

Name: ribH

Synonym: Acid_6223

Alternate gene names: 116625278

Gene position: 7802516-7802040 (Counterclockwise)

Preceding gene: 116625279

Following gene: 116625277

Centisome position: 78.29

GC content: 65.2

Gene sequence:

>477_bases
ATGCCGAAAGTATTTGAAGGCCAGTTATCGGCGGCGGGGCTGCGGTTTGCCATCGTGGTCAGCCGCTTCAACAGCTTCAT
CACGGACCGCCTGCTGGGCGGCGCGATGGATGCCCTGACGCGCGCCGGCGCCGATCCGGAACTGATCGATATTGTGAAGG
TGCCCGGCTCCTGGGAGGTTCCGCTGGTGACCGGGGAACTGGCGCGGCAACATCGTTACGATGCGCTGATCTGCCTGAGC
GCCGTGATCCGCGGCGAGACGCCGCACTTCGACTATGTCGCCGGAGAGGCGGCCAAGGGCATCGCGCATGTTTCGTCGGA
GACCGGGGTTCCGGTGGCCTTCGGCGTACTCACCACCAACACGCTGGAGCAGGCAATCGACCGCGCGGGCGCCAAAGGCG
GCAACAAGGGTTTTGACGCTGCCATGACCGCGATCGAGATGGCCAATCTCCTGCGCACGCTCCGGCAGGCGACCTAG

Upstream 100 bases:

>100_bases
GGCCGAGGCGTTCGGCGTCAGCGCGGCCACCGAAGACCGCCGCGAAGGAACGCGCGCGTTTCTGGAGAAGCGCCGCGCGG
TATTTACAGGAAAGTGAGAC

Downstream 100 bases:

>100_bases
ATGGCATCCCGCCGCAGAGCCAGGCAGCGCGCGCTGCAAATTCTCTTCATCTGGGATGCCCGCAAGCAGCCCGTGGAGGA
CGCGATCAACGCGTATTACG

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MPKVFEGQLSAAGLRFAIVVSRFNSFITDRLLGGAMDALTRAGADPELIDIVKVPGSWEVPLVTGELARQHRYDALICLS
AVIRGETPHFDYVAGEAAKGIAHVSSETGVPVAFGVLTTNTLEQAIDRAGAKGGNKGFDAAMTAIEMANLLRTLRQAT

Sequences:

>Translated_158_residues
MPKVFEGQLSAAGLRFAIVVSRFNSFITDRLLGGAMDALTRAGADPELIDIVKVPGSWEVPLVTGELARQHRYDALICLS
AVIRGETPHFDYVAGEAAKGIAHVSSETGVPVAFGVLTTNTLEQAIDRAGAKGGNKGFDAAMTAIEMANLLRTLRQAT
>Mature_157_residues
PKVFEGQLSAAGLRFAIVVSRFNSFITDRLLGGAMDALTRAGADPELIDIVKVPGSWEVPLVTGELARQHRYDALICLSA
VIRGETPHFDYVAGEAAKGIAHVSSETGVPVAFGVLTTNTLEQAIDRAGAKGGNKGFDAAMTAIEMANLLRTLRQAT

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=154, Percent_Identity=52.5974025974026, Blast_Score=167, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324429, Length=155, Percent_Identity=32.9032258064516, Blast_Score=81, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_SOLUE (Q01T71)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_827434.1
- ProteinModelPortal:   Q01T71
- SMR:   Q01T71
- STRING:   Q01T71
- GeneID:   4425559
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_6223
- NMPDR:   fig|234267.9.peg.5914
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- PhylomeDB:   Q01T71
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16711; Mature: 16580

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKVFEGQLSAAGLRFAIVVSRFNSFITDRLLGGAMDALTRAGADPELIDIVKVPGSWEV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
PLVTGELARQHRYDALICLSAVIRGETPHFDYVAGEAAKGIAHVSSETGVPVAFGVLTTN
CEECHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCHHHHHHHHHCCCCCCCEEEHHHHHH
TLEQAIDRAGAKGGNKGFDAAMTAIEMANLLRTLRQAT
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKVFEGQLSAAGLRFAIVVSRFNSFITDRLLGGAMDALTRAGADPELIDIVKVPGSWEV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
PLVTGELARQHRYDALICLSAVIRGETPHFDYVAGEAAKGIAHVSSETGVPVAFGVLTTN
CEECHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCHHHHHHHHHCCCCCCCEEEHHHHHH
TLEQAIDRAGAKGGNKGFDAAMTAIEMANLLRTLRQAT
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA