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The map label for this gene is eno

Identifier: 116623463

GI number: 116623463

Start: 5535289

End: 5536572

Strand: Direct

Name: eno

Synonym: Acid_4373

Alternate gene names: 116623463

Gene position: 5535289-5536572 (Clockwise)

Preceding gene: 116623462

Following gene: 116623464

Centisome position: 55.54

GC content: 62.93

Gene sequence:

>1284_bases
TTGACGACGATCGTACGCATCTTTGGACGAGAGATTCTGGATTCCCGCGGGAATCCGACTGTGGAAGCGGATGTCTATCT
CGCAGACGGCAGTATGGGCAGGGCCGCAGTGCCTTCCGGCGCATCCACCGGCGAGCACGAAGCCGTGGAACTTCGCGATG
GCGACAAGGGCCGCTATCTGGGCAAAGGCTGCCTGAACGCGGCCAAGCACATCAACGGCGAAATCGCCACCGCGCTGCAC
GGCAAGGACGCCACCCAGCAGGGCGAAATCGATGGCGCGATGATCGCGCTCGATGGCACGCCGAACAAAGGACGCCTCGG
CGCCAACGCCATCCTGGCCGTCTCCATGGCCACCGCCCGCGCCGCCGCCGCGGCCCAGCGCACCCCGCTCTATCGCTACC
TCGGCGGAGTCGGCGCCAACACCCTGCCCGTCCCCATGATGAACATCATCAACGGCGGCGCGCATGCTGATAACTCCGTA
GACCTGCAGGAATTCATGGTCGCCCCCTTCGGCGCCAAGAGCTTCAGCGAAGCCCTCCGCATGGGCGTCGAGGTCTTCCA
TACCCTGAAGAAGGTGCTCAGCAAGAAAGGCTACTCCACCGCGGTTGGCGACGAGGGCGGCTTCGCCCCGATGCTGAAAT
CCAACGAAGAAGCCATCGAAAGCTGCCTCGAAGCCATCACGCAGGCCGGCTTCAAACCCGGCGAGAACGTCGGCATCTGC
CTCGACCCCGCCTCCAGCGAATTCTTTAAGGGCGGCAAGTACGTCTTCAAGAAGTCCGACAAGAGTGAGCGCACCAGCGA
ACAGATGGTCGAGTTCTGGGCCAACTGGGTCCGCCAGTATCCCGCCATCCTCTCCATTGAAGATGGCATGGCGGAAGACG
ATTGGGCCGGCTGGAAGCTGCTCACCGATACCGTCGGCAGCAAGATCCAACTCGTCGGCGACGATCTGTTCGTCACCAAC
AGCACCCGCCTGAAGCAGGGCATCGAAGGCGGTGTCGCCAATTCGATTCTCGTCAAAGTCAACCAGATCGGCACCCTCAC
CGAGACCCTGGAAGCCATGCAGATGGCCGCCAATGCCGGGTATACCGCGGTGGTCTCGCACCGCTCCGGCGAAACCGAAG
ATCCCTTCATCGCGGATCTCGCCGTCGCAACCAATGCCGGCCAGATCAAGACCGGCTCCGCTAGCCGCACCGACCGCATC
TGCAAGTACAACCAGCTGCTCCGGATCGAAGAGCGCCTCGGTTCGTCGGCCGTGTTCCCCGGCAGAAAGGCCTACAGCCG
GTGA

Upstream 100 bases:

>100_bases
TACCAGGTTACCGAGGGCGGTGTCACCGTGGTCGGCAATTTCGAAGAAGGCCGCACCAAAGCGCTGCCTTACCTGGCTTA
ATTTTTTAGGGAGCAATACT

Downstream 100 bases:

>100_bases
AGAAACTCGTTCTCATCCGCCACGGCGAAAGCACGTGGAACAAAGAAAATCGCTTCACCGGCTGGACCGACGTCGATCTC
TCCGACGAAGGCCGCCAGCA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTTIVRIFGREILDSRGNPTVEADVYLADGSMGRAAVPSGASTGEHEAVELRDGDKGRYLGKGCLNAAKHINGEIATALH
GKDATQQGEIDGAMIALDGTPNKGRLGANAILAVSMATARAAAAAQRTPLYRYLGGVGANTLPVPMMNIINGGAHADNSV
DLQEFMVAPFGAKSFSEALRMGVEVFHTLKKVLSKKGYSTAVGDEGGFAPMLKSNEEAIESCLEAITQAGFKPGENVGIC
LDPASSEFFKGGKYVFKKSDKSERTSEQMVEFWANWVRQYPAILSIEDGMAEDDWAGWKLLTDTVGSKIQLVGDDLFVTN
STRLKQGIEGGVANSILVKVNQIGTLTETLEAMQMAANAGYTAVVSHRSGETEDPFIADLAVATNAGQIKTGSASRTDRI
CKYNQLLRIEERLGSSAVFPGRKAYSR

Sequences:

>Translated_427_residues
MTTIVRIFGREILDSRGNPTVEADVYLADGSMGRAAVPSGASTGEHEAVELRDGDKGRYLGKGCLNAAKHINGEIATALH
GKDATQQGEIDGAMIALDGTPNKGRLGANAILAVSMATARAAAAAQRTPLYRYLGGVGANTLPVPMMNIINGGAHADNSV
DLQEFMVAPFGAKSFSEALRMGVEVFHTLKKVLSKKGYSTAVGDEGGFAPMLKSNEEAIESCLEAITQAGFKPGENVGIC
LDPASSEFFKGGKYVFKKSDKSERTSEQMVEFWANWVRQYPAILSIEDGMAEDDWAGWKLLTDTVGSKIQLVGDDLFVTN
STRLKQGIEGGVANSILVKVNQIGTLTETLEAMQMAANAGYTAVVSHRSGETEDPFIADLAVATNAGQIKTGSASRTDRI
CKYNQLLRIEERLGSSAVFPGRKAYSR
>Mature_426_residues
TTIVRIFGREILDSRGNPTVEADVYLADGSMGRAAVPSGASTGEHEAVELRDGDKGRYLGKGCLNAAKHINGEIATALHG
KDATQQGEIDGAMIALDGTPNKGRLGANAILAVSMATARAAAAAQRTPLYRYLGGVGANTLPVPMMNIINGGAHADNSVD
LQEFMVAPFGAKSFSEALRMGVEVFHTLKKVLSKKGYSTAVGDEGGFAPMLKSNEEAIESCLEAITQAGFKPGENVGICL
DPASSEFFKGGKYVFKKSDKSERTSEQMVEFWANWVRQYPAILSIEDGMAEDDWAGWKLLTDTVGSKIQLVGDDLFVTNS
TRLKQGIEGGVANSILVKVNQIGTLTETLEAMQMAANAGYTAVVSHRSGETEDPFIADLAVATNAGQIKTGSASRTDRIC
KYNQLLRIEERLGSSAVFPGRKAYSR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=431, Percent_Identity=52.4361948955916, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI301897477, Length=429, Percent_Identity=52.2144522144522, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI301897469, Length=429, Percent_Identity=52.2144522144522, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=50.5800464037123, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI301897479, Length=427, Percent_Identity=48.2435597189695, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=24.1071428571429, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=24.1071428571429, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=24.1071428571429, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1789141, Length=427, Percent_Identity=61.5925058548009, Blast_Score=506, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=52.7777777777778, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=52.7777777777778, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=191, Percent_Identity=49.738219895288, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6323985, Length=438, Percent_Identity=48.8584474885845, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324974, Length=438, Percent_Identity=48.8584474885845, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324969, Length=438, Percent_Identity=48.8584474885845, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=50.8083140877598, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=49.8845265588915, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580918, Length=432, Percent_Identity=48.6111111111111, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580916, Length=432, Percent_Identity=48.6111111111111, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580920, Length=432, Percent_Identity=48.6111111111111, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580914, Length=432, Percent_Identity=48.6111111111111, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI281360527, Length=432, Percent_Identity=48.6111111111111, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17137654, Length=432, Percent_Identity=48.6111111111111, Blast_Score=378, Evalue=1e-105,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_SOLUE (Q01YD1)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_825619.1
- ProteinModelPortal:   Q01YD1
- SMR:   Q01YD1
- STRING:   Q01YD1
- GeneID:   4427367
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_4373
- NMPDR:   fig|234267.9.peg.4159
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q01YD1
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45455; Mature: 45324

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 339-339 BINDING 155-155 BINDING 164-164 BINDING 287-287 BINDING 314-314 BINDING 339-339 BINDING 390-390

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIVRIFGREILDSRGNPTVEADVYLADGSMGRAAVPSGASTGEHEAVELRDGDKGRYL
CCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCH
GKGCLNAAKHINGEIATALHGKDATQQGEIDGAMIALDGTPNKGRLGANAILAVSMATAR
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEHHHHHH
AAAAAQRTPLYRYLGGVGANTLPVPMMNIINGGAHADNSVDLQEFMVAPFGAKSFSEALR
HHHHHHCCHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHHHHHH
MGVEVFHTLKKVLSKKGYSTAVGDEGGFAPMLKSNEEAIESCLEAITQAGFKPGENVGIC
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEE
LDPASSEFFKGGKYVFKKSDKSERTSEQMVEFWANWVRQYPAILSIEDGMAEDDWAGWKL
ECCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCH
LTDTVGSKIQLVGDDLFVTNSTRLKQGIEGGVANSILVKVNQIGTLTETLEAMQMAANAG
HHHHCCCEEEEECCCEEEECCHHHHHHCCCCCCCEEEEEEHHHCHHHHHHHHHHHHHCCC
YTAVVSHRSGETEDPFIADLAVATNAGQIKTGSASRTDRICKYNQLLRIEERLGSSAVFP
CEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
GRKAYSR
CHHHCCC
>Mature Secondary Structure 
TTIVRIFGREILDSRGNPTVEADVYLADGSMGRAAVPSGASTGEHEAVELRDGDKGRYL
CHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCH
GKGCLNAAKHINGEIATALHGKDATQQGEIDGAMIALDGTPNKGRLGANAILAVSMATAR
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEHHHHHH
AAAAAQRTPLYRYLGGVGANTLPVPMMNIINGGAHADNSVDLQEFMVAPFGAKSFSEALR
HHHHHHCCHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHHHHHH
MGVEVFHTLKKVLSKKGYSTAVGDEGGFAPMLKSNEEAIESCLEAITQAGFKPGENVGIC
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEE
LDPASSEFFKGGKYVFKKSDKSERTSEQMVEFWANWVRQYPAILSIEDGMAEDDWAGWKL
ECCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCH
LTDTVGSKIQLVGDDLFVTNSTRLKQGIEGGVANSILVKVNQIGTLTETLEAMQMAANAG
HHHHCCCEEEEECCCEEEECCHHHHHHCCCCCCCEEEEEEHHHCHHHHHHHHHHHHHCCC
YTAVVSHRSGETEDPFIADLAVATNAGQIKTGSASRTDRICKYNQLLRIEERLGSSAVFP
CEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
GRKAYSR
CHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA