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The map label for this gene is yciL [C]

Identifier: 116623183

GI number: 116623183

Start: 5200745

End: 5201539

Strand: Direct

Name: yciL [C]

Synonym: Acid_4090

Alternate gene names: 116623183

Gene position: 5200745-5201539 (Clockwise)

Preceding gene: 116623181

Following gene: 116623185

Centisome position: 52.19

GC content: 59.12

Gene sequence:

>795_bases
ATGGAAAAGAAGCGCAATCGACAGGAAAAGATAACGTTAAAGACTTTGGAACGAGTGTTCTCCAAAGCCGGCGCGGGTTC
GCGAACGGATGCGCGGAAGTGGATTTCGGGAGGAAAGGTCCGGGTGAATGGGAAGGTGGTACTAAATCCCGATCACTGGG
TCGATCCGGATCACGACAAGGTGACGCTCGAGGGGAAGCCATTAAAGGCGGCAACAAAGAGCTATATTCTCCTGTATAAA
CCGAAAGGTTACCTGACGACGTACCGGGATCCGGAGGGCAGGCCGACGGTTTATGCGTTGTTGGGGGATGTGGGGAAGTG
GCTCTCGCCGGTGGGCAGGCTCGACCTGGATACGAGCGGGCTGCTGGTGCTGACGAACGACAACGATTTCGCGGAGCGAA
TCACGAACCCGGAGCACAAGGTTCCGAAGACGTATCAGATCAAGGCGGCGACGGTATTGAGCGATGAACAGATCGAGCGG
CTACGGGCGGGAGTGGAGTTGAGCGACGGGCCGACGCGTCCGGCGGAGGTGAAGCGGCTGCGCGACGGGCCGAAGCACAC
GTTCCTGGAGATGACGATTACGGAAGGGCGGAACAGGCAGGTGAGGCGGATGCTGGAGGCGGTGGAGAGTAAGGTGTCGA
AGCTGGTGCGGACGGCGATCGGGCCGGTGCGGATCGGTGAACTGCCGATCGGCAAGTGGCGGAATTTGACGGCGGATGAG
GTACATGAGCTACGCGGGCAGGCGGCGAAGAAGGCAAGCGGGCCGGCGAGAGACAAGAATAGAAATAAGTATTGA

Upstream 100 bases:

>100_bases
ATGCAAGGTCAAAAGGCAGCGGGCACCTCGAATTAGAATTGAGTCCAGGGAAATCCTTATTCCAATGTAACAATAGTACT
AGTCTTAACAATTTAAATTT

Downstream 100 bases:

>100_bases
GAAACGCCGGGATATTGAAGACCGCGGTGGCCAGAGGAGGAGGCGGCGGTGACGCACGATTTGGTAGCGCGGGGGTTCTC
CGAGGAAGCCGCCTGAAAGG

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MEKKRNRQEKITLKTLERVFSKAGAGSRTDARKWISGGKVRVNGKVVLNPDHWVDPDHDKVTLEGKPLKAATKSYILLYK
PKGYLTTYRDPEGRPTVYALLGDVGKWLSPVGRLDLDTSGLLVLTNDNDFAERITNPEHKVPKTYQIKAATVLSDEQIER
LRAGVELSDGPTRPAEVKRLRDGPKHTFLEMTITEGRNRQVRRMLEAVESKVSKLVRTAIGPVRIGELPIGKWRNLTADE
VHELRGQAAKKASGPARDKNRNKY

Sequences:

>Translated_264_residues
MEKKRNRQEKITLKTLERVFSKAGAGSRTDARKWISGGKVRVNGKVVLNPDHWVDPDHDKVTLEGKPLKAATKSYILLYK
PKGYLTTYRDPEGRPTVYALLGDVGKWLSPVGRLDLDTSGLLVLTNDNDFAERITNPEHKVPKTYQIKAATVLSDEQIER
LRAGVELSDGPTRPAEVKRLRDGPKHTFLEMTITEGRNRQVRRMLEAVESKVSKLVRTAIGPVRIGELPIGKWRNLTADE
VHELRGQAAKKASGPARDKNRNKY
>Mature_264_residues
MEKKRNRQEKITLKTLERVFSKAGAGSRTDARKWISGGKVRVNGKVVLNPDHWVDPDHDKVTLEGKPLKAATKSYILLYK
PKGYLTTYRDPEGRPTVYALLGDVGKWLSPVGRLDLDTSGLLVLTNDNDFAERITNPEHKVPKTYQIKAATVLSDEQIER
LRAGVELSDGPTRPAEVKRLRDGPKHTFLEMTITEGRNRQVRRMLEAVESKVSKLVRTAIGPVRIGELPIGKWRNLTADE
VHELRGQAAKKASGPARDKNRNKY

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=238, Percent_Identity=34.453781512605, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1788510, Length=231, Percent_Identity=35.0649350649351, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1790453, Length=233, Percent_Identity=33.4763948497854, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI87081838, Length=177, Percent_Identity=35.5932203389831, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 29708; Mature: 29708

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKRNRQEKITLKTLERVFSKAGAGSRTDARKWISGGKVRVNGKVVLNPDHWVDPDHDK
CCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEECCEEEECCCCCCCCCCCE
VTLEGKPLKAATKSYILLYKPKGYLTTYRDPEGRPTVYALLGDVGKWLSPVGRLDLDTSG
EEECCCCCCCCCCCEEEEECCCCCEEECCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCC
LLVLTNDNDFAERITNPEHKVPKTYQIKAATVLSDEQIERLRAGVELSDGPTRPAEVKRL
EEEEECCCHHHHHCCCCCCCCCCEEEEEEEEECCHHHHHHHHCCCCCCCCCCCHHHHHHH
RDGPKHTFLEMTITEGRNRQVRRMLEAVESKVSKLVRTAIGPVRIGELPIGKWRNLTADE
HCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHH
VHELRGQAAKKASGPARDKNRNKY
HHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MEKKRNRQEKITLKTLERVFSKAGAGSRTDARKWISGGKVRVNGKVVLNPDHWVDPDHDK
CCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEECCEEEECCCCCCCCCCCE
VTLEGKPLKAATKSYILLYKPKGYLTTYRDPEGRPTVYALLGDVGKWLSPVGRLDLDTSG
EEECCCCCCCCCCCEEEEECCCCCEEECCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCC
LLVLTNDNDFAERITNPEHKVPKTYQIKAATVLSDEQIERLRAGVELSDGPTRPAEVKRL
EEEEECCCHHHHHCCCCCCCCCCEEEEEEEEECCHHHHHHHHCCCCCCCCCCCHHHHHHH
RDGPKHTFLEMTITEGRNRQVRRMLEAVESKVSKLVRTAIGPVRIGELPIGKWRNLTADE
HCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHH
VHELRGQAAKKASGPARDKNRNKY
HHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]