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The map label for this gene is ycjG [C]

Identifier: 116622524

GI number: 116622524

Start: 4328583

End: 4329677

Strand: Direct

Name: ycjG [C]

Synonym: Acid_3422

Alternate gene names: 116622524

Gene position: 4328583-4329677 (Clockwise)

Preceding gene: 116622523

Following gene: 116622525

Centisome position: 43.44

GC content: 61.0

Gene sequence:

>1095_bases
ATGAATCGCCGCAATTTTATGAGAACCGCCGCCATCGCGCCCGCTGCCGGCGCATTCGCCGCGCCGGCCACGGGCAGCGC
GGCTTTTACGAGCAAGATCACCAGGTTGAATTTAAAACACACGTGGACGACCACGATGTCTTCCAGCCAGTATCGCGACA
CGCTGCACACGGCATACACGCGCGGCGGCATCACGGGCCACGGTGAGGGGGCGCCGATCGTGCGCTATCACGAGGACGCG
GCGGGAGCGCAGAAGGCGGTGGAGGGCGTGCGCGACCTGCTGCTCTCGGCGGACCCGATGCAATTTTCCAAAGTGATGGC
CGAGGTGTTCAAACGCATTCCGGGCGAGTGGGCGGGGAAGGCGGCAATCGATATCGCGCTGATGGACTGGGTGGGGCAGA
AGCTGGGGATTCCGTTATACACGTATTTCGGACTCGACCCGAAGGACGCACCGCTGACGACGTTTTCGATCGGCATCGAC
ACGCCGGAGATCACCAAGCAGAAGACGCTGGAGGCGGCGGACTACCCGGTGCTGAAGGTGAAGGTCGGTCTGGCCACCGA
CGAGCCGACGATCGAGGCGGTGCGGAGCGTGACCAAGAAGCCGATTCGCGTGGATGCGAATGAGGGATGGAAGGACAAAG
AAGAGGCGGTGCGCAAGATCAACTGGCTGGAGAAGATGGGCGTGGAGTTCATCGAGCAGCCGATGCCGGCTGAGATGTTC
GAAGAGAACAAGTATGTGCGCAGCAAGGTACACATCCCGGTGATCGCCGACGAGGCGTGCCAGCGGGCGAGCGACATTCC
GAAGTTGAAGGAAGCGTACGACGGGGTCAACGTAAAGCTGGATAAGAGCGGCGGCATGCTGGAGGCATACCGCATGCTCA
TGATCGCGAAGGCGCTGGGAATGAAGACCATGCTGGGGTGCATGATTTCGAGTTCGGTAAGTGTGACGGCGGCGGCGCAC
TTATCGCCGTTGGTCGATTACTCGGACTTAGACGGCAACCTATTGATCTCCAACGATCCCTTTCACGGAGTGCGGGTGGA
GAAGGGTAAGTTGATATTGCCGAAGGGCCCGGGGCTCGGGTTGACGCCCGCCTAA

Upstream 100 bases:

>100_bases
AGTTTATCAACGCAACCACTCACCTGACTCCGATGATCCGGATCGACGACCTTAATGACGCGTACTGGACGAAGTTGCTA
GTGGCTGCCAGGAGAGTGAA

Downstream 100 bases:

>100_bases
CTCCGGGTTTTCCGCGATGAAGCCCTGGACGCTGCTCGACCACACGGTCACGCCGGACGGAAAGAACATCACGCTGCACG
AGCACGACGGCGCGTATGCC

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MNRRNFMRTAAIAPAAGAFAAPATGSAAFTSKITRLNLKHTWTTTMSSSQYRDTLHTAYTRGGITGHGEGAPIVRYHEDA
AGAQKAVEGVRDLLLSADPMQFSKVMAEVFKRIPGEWAGKAAIDIALMDWVGQKLGIPLYTYFGLDPKDAPLTTFSIGID
TPEITKQKTLEAADYPVLKVKVGLATDEPTIEAVRSVTKKPIRVDANEGWKDKEEAVRKINWLEKMGVEFIEQPMPAEMF
EENKYVRSKVHIPVIADEACQRASDIPKLKEAYDGVNVKLDKSGGMLEAYRMLMIAKALGMKTMLGCMISSSVSVTAAAH
LSPLVDYSDLDGNLLISNDPFHGVRVEKGKLILPKGPGLGLTPA

Sequences:

>Translated_364_residues
MNRRNFMRTAAIAPAAGAFAAPATGSAAFTSKITRLNLKHTWTTTMSSSQYRDTLHTAYTRGGITGHGEGAPIVRYHEDA
AGAQKAVEGVRDLLLSADPMQFSKVMAEVFKRIPGEWAGKAAIDIALMDWVGQKLGIPLYTYFGLDPKDAPLTTFSIGID
TPEITKQKTLEAADYPVLKVKVGLATDEPTIEAVRSVTKKPIRVDANEGWKDKEEAVRKINWLEKMGVEFIEQPMPAEMF
EENKYVRSKVHIPVIADEACQRASDIPKLKEAYDGVNVKLDKSGGMLEAYRMLMIAKALGMKTMLGCMISSSVSVTAAAH
LSPLVDYSDLDGNLLISNDPFHGVRVEKGKLILPKGPGLGLTPA
>Mature_364_residues
MNRRNFMRTAAIAPAAGAFAAPATGSAAFTSKITRLNLKHTWTTTMSSSQYRDTLHTAYTRGGITGHGEGAPIVRYHEDA
AGAQKAVEGVRDLLLSADPMQFSKVMAEVFKRIPGEWAGKAAIDIALMDWVGQKLGIPLYTYFGLDPKDAPLTTFSIGID
TPEITKQKTLEAADYPVLKVKVGLATDEPTIEAVRSVTKKPIRVDANEGWKDKEEAVRKINWLEKMGVEFIEQPMPAEMF
EENKYVRSKVHIPVIADEACQRASDIPKLKEAYDGVNVKLDKSGGMLEAYRMLMIAKALGMKTMLGCMISSSVSVTAAAH
LSPLVDYSDLDGNLLISNDPFHGVRVEKGKLILPKGPGLGLTPA

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the epimerization of L-Ala-D-Asp [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=295, Percent_Identity=31.864406779661, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 39608; Mature: 39608

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRNFMRTAAIAPAAGAFAAPATGSAAFTSKITRLNLKHTWTTTMSSSQYRDTLHTAYT
CCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHHH
RGGITGHGEGAPIVRYHEDAAGAQKAVEGVRDLLLSADPMQFSKVMAEVFKRIPGEWAGK
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
AAIDIALMDWVGQKLGIPLYTYFGLDPKDAPLTTFSIGIDTPEITKQKTLEAADYPVLKV
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEE
KVGLATDEPTIEAVRSVTKKPIRVDANEGWKDKEEAVRKINWLEKMGVEFIEQPMPAEMF
EEECCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCHHHH
EENKYVRSKVHIPVIADEACQRASDIPKLKEAYDGVNVKLDKSGGMLEAYRMLMIAKALG
HCCCHHHHEEEEEEECHHHHHHHHCCHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHH
MKTMLGCMISSSVSVTAAAHLSPLVDYSDLDGNLLISNDPFHGVRVEKGKLILPKGPGLG
HHHHHHHHHCCCCCEEEHHHHHHCCCCCCCCCCEEEECCCCCCEEEECCCEEEECCCCCC
LTPA
CCCC
>Mature Secondary Structure
MNRRNFMRTAAIAPAAGAFAAPATGSAAFTSKITRLNLKHTWTTTMSSSQYRDTLHTAYT
CCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHHH
RGGITGHGEGAPIVRYHEDAAGAQKAVEGVRDLLLSADPMQFSKVMAEVFKRIPGEWAGK
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
AAIDIALMDWVGQKLGIPLYTYFGLDPKDAPLTTFSIGIDTPEITKQKTLEAADYPVLKV
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEE
KVGLATDEPTIEAVRSVTKKPIRVDANEGWKDKEEAVRKINWLEKMGVEFIEQPMPAEMF
EEECCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCHHHH
EENKYVRSKVHIPVIADEACQRASDIPKLKEAYDGVNVKLDKSGGMLEAYRMLMIAKALG
HCCCHHHHEEEEEEECHHHHHHHHCCHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHH
MKTMLGCMISSSVSVTAAAHLSPLVDYSDLDGNLLISNDPFHGVRVEKGKLILPKGPGLG
HHHHHHHHHCCCCCEEEHHHHHHCCCCCCCCCCEEEECCCCCCEEEECCCEEEECCCCCC
LTPA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]