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The map label for this gene is ykfC [H]

Identifier: 116622523

GI number: 116622523

Start: 4327753

End: 4328586

Strand: Direct

Name: ykfC [H]

Synonym: Acid_3421

Alternate gene names: 116622523

Gene position: 4327753-4328586 (Clockwise)

Preceding gene: 116622522

Following gene: 116622524

Centisome position: 43.43

GC content: 61.15

Gene sequence:

>834_bases
ATGCCAGGAATAGCCGCACTTGCCCTGTTTGCCGCAGCGCTGCCGAACGCAGTAGTGCTGCAGCCGGTGGCCAATATGTA
TTCGCGTCCGAGCGCTGACGCCGACGTAGTCAGCCAGGCGATTTACGGCGCGAACGTGAACCTGATCGAGGAAAAGGACG
GGTGGGCGCACATCCGGACCGCGGATGATTACACCGGGTGGACGCCGCTCTCCGCGCTGCTGCCGGGGAAGGCGTACGCC
ACGAGCGGCCGCGTAGGCGAGGTGCAGAGCCTGTTTGCGCATATCTACCGGGAAGCGAGCGTGACGCGGCATGCTCCGCT
GGTGACGGTGCCGTTCGAGGTCAAACTCGAGGTGCTGACCGAACCCAAAGAGGATACGCGATGGTTCCAGGTGCGACTGC
CTGACGATCGAGCGGGCTGGATTCAGGCGGGGGATATCTCGCTGGCGCCGAAGACGATGTCGATCTCCGAGACACTGGAG
TTTTCGAAACGCTTTCTCGGGCTGCCTTATACGTGGGGCGGGACTTCGAGCTACGGGTACGATTGCTCGGGGTTTTCGCA
AATGCTGGGACGGCGGCGCGGGGTCAATATGCCGCGCGATGCGCAGCCGCAGGCGGAGTGGAGCGGCGTCGCGCCGGTGG
ACCGGAAGGATCTTCAGGCCGGCGACTTACTCTATTTCGGCAGTTCCGAAAAGAAGATTACGCATACCGGAATCTACATG
GGCGATGGGAAGTTTATCAACGCAACCACTCACCTGACTCCGATGATCCGGATCGACGACCTTAATGACGCGTACTGGAC
GAAGTTGCTAGTGGCTGCCAGGAGAGTGAAATGA

Upstream 100 bases:

>100_bases
CTATTATGCGCCTTCGCAGTAGGTAACGCGCGGTCTTGGGCAAGAGACTGTTGGAAGAGCGCGTCCCACTGTGATGATAG
GCTGGTATATATAACCCTTT

Downstream 100 bases:

>100_bases
ATCGCCGCAATTTTATGAGAACCGCCGCCATCGCGCCCGCTGCCGGCGCATTCGCCGCGCCGGCCACGGGCAGCGCGGCT
TTTACGAGCAAGATCACCAG

Product: NLP/P60 protein

Products: NA

Alternate protein names: Cell wall endopeptidase ykfC [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MPGIAALALFAAALPNAVVLQPVANMYSRPSADADVVSQAIYGANVNLIEEKDGWAHIRTADDYTGWTPLSALLPGKAYA
TSGRVGEVQSLFAHIYREASVTRHAPLVTVPFEVKLEVLTEPKEDTRWFQVRLPDDRAGWIQAGDISLAPKTMSISETLE
FSKRFLGLPYTWGGTSSYGYDCSGFSQMLGRRRGVNMPRDAQPQAEWSGVAPVDRKDLQAGDLLYFGSSEKKITHTGIYM
GDGKFINATTHLTPMIRIDDLNDAYWTKLLVAARRVK

Sequences:

>Translated_277_residues
MPGIAALALFAAALPNAVVLQPVANMYSRPSADADVVSQAIYGANVNLIEEKDGWAHIRTADDYTGWTPLSALLPGKAYA
TSGRVGEVQSLFAHIYREASVTRHAPLVTVPFEVKLEVLTEPKEDTRWFQVRLPDDRAGWIQAGDISLAPKTMSISETLE
FSKRFLGLPYTWGGTSSYGYDCSGFSQMLGRRRGVNMPRDAQPQAEWSGVAPVDRKDLQAGDLLYFGSSEKKITHTGIYM
GDGKFINATTHLTPMIRIDDLNDAYWTKLLVAARRVK
>Mature_276_residues
PGIAALALFAAALPNAVVLQPVANMYSRPSADADVVSQAIYGANVNLIEEKDGWAHIRTADDYTGWTPLSALLPGKAYAT
SGRVGEVQSLFAHIYREASVTRHAPLVTVPFEVKLEVLTEPKEDTRWFQVRLPDDRAGWIQAGDISLAPKTMSISETLEF
SKRFLGLPYTWGGTSSYGYDCSGFSQMLGRRRGVNMPRDAQPQAEWSGVAPVDRKDLQAGDLLYFGSSEKKITHTGIYMG
DGKFINATTHLTPMIRIDDLNDAYWTKLLVAARRVK

Specific function: Specifically hydrolyzes gamma-D-glutamyl-L-lysine bonds in murein peptides, releasing L-Ala-D-Glu [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=118, Percent_Identity=34.7457627118644, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788501, Length=113, Percent_Identity=38.0530973451327, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786421, Length=118, Percent_Identity=35.5932203389831, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788001, Length=113, Percent_Identity=35.3982300884956, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 30498; Mature: 30367

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGIAALALFAAALPNAVVLQPVANMYSRPSADADVVSQAIYGANVNLIEEKDGWAHIRT
CCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCCCEEEEEE
ADDYTGWTPLSALLPGKAYATSGRVGEVQSLFAHIYREASVTRHAPLVTVPFEVKLEVLT
CCCCCCCCCHHHHCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCEEEEEEEC
EPKEDTRWFQVRLPDDRAGWIQAGDISLAPKTMSISETLEFSKRFLGLPYTWGGTSSYGY
CCCCCCEEEEEECCCCCCCCEEECCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
DCSGFSQMLGRRRGVNMPRDAQPQAEWSGVAPVDRKDLQAGDLLYFGSSEKKITHTGIYM
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEEE
GDGKFINATTHLTPMIRIDDLNDAYWTKLLVAARRVK
CCCEEEEEEECCEEEEEEECCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PGIAALALFAAALPNAVVLQPVANMYSRPSADADVVSQAIYGANVNLIEEKDGWAHIRT
CHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCCCEEEEEE
ADDYTGWTPLSALLPGKAYATSGRVGEVQSLFAHIYREASVTRHAPLVTVPFEVKLEVLT
CCCCCCCCCHHHHCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCEEEEEEEC
EPKEDTRWFQVRLPDDRAGWIQAGDISLAPKTMSISETLEFSKRFLGLPYTWGGTSSYGY
CCCCCCEEEEEECCCCCCCCEEECCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
DCSGFSQMLGRRRGVNMPRDAQPQAEWSGVAPVDRKDLQAGDLLYFGSSEKKITHTGIYM
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEEE
GDGKFINATTHLTPMIRIDDLNDAYWTKLLVAARRVK
CCCEEEEEEECCEEEEEEECCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]