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The map label for this gene is pemI [H]

Identifier: 116621651

GI number: 116621651

Start: 3212141

End: 3212404

Strand: Reverse

Name: pemI [H]

Synonym: Acid_2533

Alternate gene names: 116621651

Gene position: 3212404-3212141 (Counterclockwise)

Preceding gene: 116621653

Following gene: 116621650

Centisome position: 32.23

GC content: 64.39

Gene sequence:

>264_bases
ATGCACACAACAAATCTGCGGAAGGTCGGAGGCTCGGTCATGCTGGCGGTTCCCCCCGCGCTACTCGATATCCTGCGCCT
GCAACCTGGGGTCAGAGTCGGCATCACGATTGAGCGCGGGCGACTCGTCGTGGAACCGCGGCAGCGGCCCCGCTATACAC
TGGACGAACTCCTTGCTCAATGCGATCCGAAGGCAGCGCGCAAGCGCACCAGGGAGGATCGGGAATGGCTGGACAGCAAG
CCCGCGGGCGCCGAGCTCATCTGA

Upstream 100 bases:

>100_bases
TTTGGGGAAGGGGGGTTTGGGAGCTTGGAGGGTTCATAGGAATCCGTTGTCAGGCGGGACTCTCGAAATGTTATACTTGG
ATATAATCCGGACAACTGCT

Downstream 100 bases:

>100_bases
TGAAGCGCGGCGAAATCTGGCTGGTCGGCCTCGATCCGGTTGAAGGGCACGAGCAGAAGGGCCGCCGGCCCGTGCTGATC
GTTTCGCCGGAAGCGTTCAA

Product: transcriptional regulator/antitoxin, MazE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 87; Mature: 87

Protein sequence:

>87_residues
MHTTNLRKVGGSVMLAVPPALLDILRLQPGVRVGITIERGRLVVEPRQRPRYTLDELLAQCDPKAARKRTREDREWLDSK
PAGAELI

Sequences:

>Translated_87_residues
MHTTNLRKVGGSVMLAVPPALLDILRLQPGVRVGITIERGRLVVEPRQRPRYTLDELLAQCDPKAARKRTREDREWLDSK
PAGAELI
>Mature_87_residues
MHTTNLRKVGGSVMLAVPPALLDILRLQPGVRVGITIERGRLVVEPRQRPRYTLDELLAQCDPKAARKRTREDREWLDSK
PAGAELI

Specific function: Responsible for the stable maintenance of the plasmid during cell division. Both PemI and PemK proteins bind to the promoter region of the pem operon to autoregulate their synthesis [H]

COG id: COG2336

COG function: function code T; Growth regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PemI family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007159 [H]

Pfam domain/function: PF04014 SpoVT_AbrB [H]

EC number: NA

Molecular weight: Translated: 9810; Mature: 9810

Theoretical pI: Translated: 10.78; Mature: 10.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTTNLRKVGGSVMLAVPPALLDILRLQPGVRVGITIERGRLVVEPRQRPRYTLDELLAQ
CCCCCCHHCCCEEEEEECHHHHHHHHCCCCCEEEEEEECCEEEECCCCCCCCCHHHHHHH
CDPKAARKRTREDREWLDSKPAGAELI
CCCHHHHHHCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MHTTNLRKVGGSVMLAVPPALLDILRLQPGVRVGITIERGRLVVEPRQRPRYTLDELLAQ
CCCCCCHHCCCEEEEEECHHHHHHHHCCCCCEEEEEEECCEEEECCCCCCCCCHHHHHHH
CDPKAARKRTREDREWLDSKPAGAELI
CCCHHHHHHCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3019092; 3323833; 2832364; 8455570 [H]