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The map label for this gene is pemK [H]

Identifier: 116621650

GI number: 116621650

Start: 3211809

End: 3212141

Strand: Reverse

Name: pemK [H]

Synonym: Acid_2532

Alternate gene names: 116621650

Gene position: 3212141-3211809 (Counterclockwise)

Preceding gene: 116621651

Following gene: 116621647

Centisome position: 32.23

GC content: 63.96

Gene sequence:

>333_bases
ATGAAGCGCGGCGAAATCTGGCTGGTCGGCCTCGATCCGGTTGAAGGGCACGAGCAGAAGGGCCGCCGGCCCGTGCTGAT
CGTTTCGCCGGAAGCGTTCAACCACGTAACCAAGGTGCCCGTGGTTCTCCCGATCACCAGCGGCGGGAATTTCGCGCGCA
CGGCCGGATTCGCAGTGCCCCTTACTGGCGCAGGTACCAAGACAACGGGCGTGGTCCGGTGCGATCAACCCCGCGCGCTC
GATCTCGGCGCACGCGGCGGTAAGAGACTGGAAAGCGTACCTGGCGTGATCATGGATGACGTGCTGGCGAGACTGGCGCC
TATCTTTGAGTGA

Upstream 100 bases:

>100_bases
ACGAACTCCTTGCTCAATGCGATCCGAAGGCAGCGCGCAAGCGCACCAGGGAGGATCGGGAATGGCTGGACAGCAAGCCC
GCGGGCGCCGAGCTCATCTG

Downstream 100 bases:

>100_bases
GCGGATGAAGGCGTTTTAGGGTGCGATCAACTCCACGCTGTCGATCTCAGCACGTTCGGCAAGAAACCAAAAGGCTCTCC
CGGGCGGGATCATGGTAGCC

Product: transcriptional modulator of MazE/toxin, MazF

Products: NA

Alternate protein names: Endoribonuclease PemK; Kid toxin protein [H]

Number of amino acids: Translated: 110; Mature: 110

Protein sequence:

>110_residues
MKRGEIWLVGLDPVEGHEQKGRRPVLIVSPEAFNHVTKVPVVLPITSGGNFARTAGFAVPLTGAGTKTTGVVRCDQPRAL
DLGARGGKRLESVPGVIMDDVLARLAPIFE

Sequences:

>Translated_110_residues
MKRGEIWLVGLDPVEGHEQKGRRPVLIVSPEAFNHVTKVPVVLPITSGGNFARTAGFAVPLTGAGTKTTGVVRCDQPRAL
DLGARGGKRLESVPGVIMDDVLARLAPIFE
>Mature_110_residues
MKRGEIWLVGLDPVEGHEQKGRRPVLIVSPEAFNHVTKVPVVLPITSGGNFARTAGFAVPLTGAGTKTTGVVRCDQPRAL
DLGARGGKRLESVPGVIMDDVLARLAPIFE

Specific function: Part of a toxin-antitoxin (TA) module. Probably functions as an mRNA interferase. Responsible for the stable maintenance of the plasmid during cell division by postsegregational killing of plasmid-less daughter cells. Both pemI and PemK proteins bind to t

COG id: COG2337

COG function: function code T; Growth inhibitor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PemK/MazF family [H]

Homologues:

Organism=Escherichia coli, GI1790672, Length=111, Percent_Identity=45.945945945946, Blast_Score=93, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003477
- InterPro:   IPR011067 [H]

Pfam domain/function: PF02452 PemK [H]

EC number: NA

Molecular weight: Translated: 11681; Mature: 11681

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRGEIWLVGLDPVEGHEQKGRRPVLIVSPEAFNHVTKVPVVLPITSGGNFARTAGFAVP
CCCCCEEEEECCCCCCCCCCCCCCEEEECCHHHCCEECCCEEEEEECCCCEEECCCEEEE
LTGAGTKTTGVVRCDQPRALDLGARGGKRLESVPGVIMDDVLARLAPIFE
ECCCCCCCCEEEEECCCCEEEECCCCCCCCHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRGEIWLVGLDPVEGHEQKGRRPVLIVSPEAFNHVTKVPVVLPITSGGNFARTAGFAVP
CCCCCEEEEECCCCCCCCCCCCCCEEEECCHHHCCEECCCEEEEEECCCCEEECCCEEEE
LTGAGTKTTGVVRCDQPRALDLGARGGKRLESVPGVIMDDVLARLAPIFE
ECCCCCCCCEEEEECCCCEEEECCCCCCCCHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3019092; 3323833; 2832364; 8455570 [H]