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The map label for this gene is potI [H]

Identifier: 116621487

GI number: 116621487

Start: 3019256

End: 3020014

Strand: Direct

Name: potI [H]

Synonym: Acid_2369

Alternate gene names: 116621487

Gene position: 3019256-3020014 (Clockwise)

Preceding gene: 116621486

Following gene: 116621488

Centisome position: 30.3

GC content: 63.11

Gene sequence:

>759_bases
ATGAAGCGCTGGCTCGGAGTGTACGCGATCGCCTTCTTCGCCTTTCTGCACCTGCCGCTGTTGACGCTCGCCCTCTTCAG
TTTCAATTCCTCGAAGTTCACCAGGTGGGAGCACTTCTCGCTGGCCTGGTACGGCGCCATTTTCAAAGATCCGCAGATTA
TGGAAGGCCTGTGGAACAGCGTGATTATCGCGTTGTGGGCCACGGTGCTGTCTACCATCATCGGCACGCTGTGCGCGTAC
GGCCTTTGGAAGCGCGGATCCCGCCTGCTGACCGGGTCGCTCTACCTTTCGCTGGTCACGCCGGAGATCGTCACCGGCAT
TTCGCTGCTCGCGCTGTTTGAATGGGCCTTCCGCTGGCTGCACTGGCAGCTCGGGCTTTACACGGTGGTGCTGGCGCACG
TGGCCTTCTCGATCGCATACGTGGTGATCGTGGTCTCGGCGCGGCTGCGCACCGTCAGCCCCGCGCTCGAAGAGGCGGCA
ATGGATTTGGGCGCCAACGAGTGGCAGGCCTTCTGGCACGTCACGCTGCCGGCGCTCGCGCCGGGCATCGTCGCCGCCGC
CCTGCTCGCGCTCACCGTGTCCTTCGACGACTACGTCATCACCAGCATGGTAGCCGGCGTCGATTCCGAGACGCTGCCGA
TGGTGATCTACGCCATGGCGCGCCGCGGTGCGAGCCCCGTGATCAATGCCATCTCGGCCTTGATCACACTGGTCTTCGGA
GCGCTTATCCTGGTTTCAGAGCGGATGCGGGAAGCATGA

Upstream 100 bases:

>100_bases
CGCGCGACTGGCCGTTCGGTTCCGCGATTTCGATCGCGCTCATGGCCATCGTCACGCTGCTGCTGGTGATCTTCCTGCGG
CGCGAAAGCGAGTCGCTGGT

Downstream 100 bases:

>100_bases
ACCGGCGCTACTTCATCTTCGGACTGGCCGGCCTGGCCGGCTGTGCGCGCGATGCGCGTCCGCGGTTGAATGTGTACAAC
TGGTCGTCCTATATCGCCCC

Product: binding-protein-dependent transporter inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKRWLGVYAIAFFAFLHLPLLTLALFSFNSSKFTRWEHFSLAWYGAIFKDPQIMEGLWNSVIIALWATVLSTIIGTLCAY
GLWKRGSRLLTGSLYLSLVTPEIVTGISLLALFEWAFRWLHWQLGLYTVVLAHVAFSIAYVVIVVSARLRTVSPALEEAA
MDLGANEWQAFWHVTLPALAPGIVAAALLALTVSFDDYVITSMVAGVDSETLPMVIYAMARRGASPVINAISALITLVFG
ALILVSERMREA

Sequences:

>Translated_252_residues
MKRWLGVYAIAFFAFLHLPLLTLALFSFNSSKFTRWEHFSLAWYGAIFKDPQIMEGLWNSVIIALWATVLSTIIGTLCAY
GLWKRGSRLLTGSLYLSLVTPEIVTGISLLALFEWAFRWLHWQLGLYTVVLAHVAFSIAYVVIVVSARLRTVSPALEEAA
MDLGANEWQAFWHVTLPALAPGIVAAALLALTVSFDDYVITSMVAGVDSETLPMVIYAMARRGASPVINAISALITLVFG
ALILVSERMREA
>Mature_252_residues
MKRWLGVYAIAFFAFLHLPLLTLALFSFNSSKFTRWEHFSLAWYGAIFKDPQIMEGLWNSVIIALWATVLSTIIGTLCAY
GLWKRGSRLLTGSLYLSLVTPEIVTGISLLALFEWAFRWLHWQLGLYTVVLAHVAFSIAYVVIVVSARLRTVSPALEEAA
MDLGANEWQAFWHVTLPALAPGIVAAALLALTVSFDDYVITSMVAGVDSETLPMVIYAMARRGASPVINAISALITLVFG
ALILVSERMREA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=241, Percent_Identity=41.49377593361, Blast_Score=150, Evalue=9e-38,
Organism=Escherichia coli, GI1787368, Length=225, Percent_Identity=33.3333333333333, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1787714, Length=231, Percent_Identity=34.6320346320346, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788764, Length=212, Percent_Identity=28.3018867924528, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 27858; Mature: 27858

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRWLGVYAIAFFAFLHLPLLTLALFSFNSSKFTRWEHFSLAWYGAIFKDPQIMEGLWNS
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
VIIALWATVLSTIIGTLCAYGLWKRGSRLLTGSLYLSLVTPEIVTGISLLALFEWAFRWL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HWQLGLYTVVLAHVAFSIAYVVIVVSARLRTVSPALEEAAMDLGANEWQAFWHVTLPALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEHHHHHH
PGIVAAALLALTVSFDDYVITSMVAGVDSETLPMVIYAMARRGASPVINAISALITLVFG
HHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ALILVSERMREA
HHHHHHHHHHCC
>Mature Secondary Structure
MKRWLGVYAIAFFAFLHLPLLTLALFSFNSSKFTRWEHFSLAWYGAIFKDPQIMEGLWNS
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
VIIALWATVLSTIIGTLCAYGLWKRGSRLLTGSLYLSLVTPEIVTGISLLALFEWAFRWL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HWQLGLYTVVLAHVAFSIAYVVIVVSARLRTVSPALEEAAMDLGANEWQAFWHVTLPALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEHHHHHH
PGIVAAALLALTVSFDDYVITSMVAGVDSETLPMVIYAMARRGASPVINAISALITLVFG
HHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ALILVSERMREA
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]