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The map label for this gene is potD [H]

Identifier: 116621488

GI number: 116621488

Start: 3020011

End: 3021033

Strand: Direct

Name: potD [H]

Synonym: Acid_2370

Alternate gene names: 116621488

Gene position: 3020011-3021033 (Clockwise)

Preceding gene: 116621487

Following gene: 116621490

Centisome position: 30.3

GC content: 66.67

Gene sequence:

>1023_bases
ATGAACCGGCGCTACTTCATCTTCGGACTGGCCGGCCTGGCCGGCTGTGCGCGCGATGCGCGTCCGCGGTTGAATGTGTA
CAACTGGTCGTCCTATATCGCCCCCGAGACAGTCCCGGCCTTCGAAGCGGAGTTCGGCGTGCGCGTGCGTTACGCCACCT
ACGAGAGCAATGAAGAGATGCTCGCCAAGGTGATCACGGGTAACTCGGGTTGGGATATCGTGGTGCCCACCCACAGCCGC
ATCGAACCGATGCGCGGCGCGGGCCTGCTCGCGGCGCTCGACCACGCGGCGCTGCCCGGACTGGCCAATCTTGACCCGCG
CTTCCGTGCTCCCGTGTGGGACCCGGCGCTGCAATGGTGCGTCCCCTACATGTGGAACGGCACCGGCGTGGTTTACAACA
AATCGCTACGCCCGGCGCCGCAGCGGTGGGCCGACCTGTGGAGTCCGCAGTTGAAGGGCCGCCTGACAATGCTGGACGAC
CCCGAGGACATGCTCGGCGCCTGCTTGAAGAAGCTCTCGCTCTCCTTCAATGCCACCGACCCCGCGCAGTTGCAGCGGGC
GGAACACGAGGCGATCGCGCAAAAGCCGCTCCTGCGGGCGTACTTGAATGCCGAGGTGCGCGACCAGTTGGTGGCGGGCG
ATGTCCTCGCCGCGCAGCTGTGGTCTACCACCGCGCAGCAGGCGATCGATGCCGCGCCGCAGCTGGCCTTCGCGTATCCC
GCGGAAGGCTTCCCGTTCTATTGCGATTGCGCCGTGATCTTGCGCGAGAGCCGCCGCCCGGAACTGGCGCATAAATTCCT
GAACTTCCTGCTGCGCCCGCCGGTCTCCGCGCAGATCGTGCAGGCCACCCGGACTGCGACGGCGAATGGCGCCGCTCTCA
GCCTGCTGCCGGAAGATACGCGCAACAATCCGGCGCTCTACCCCGGCCCCGAAATCTACGCGCGCGGCGAGTGGCCCCAC
CCCGTCCCCAACGCCACGCAGCGCCTCCGCGACCGCATCTGGACAGAAATCAAGAGTGCGTAA

Upstream 100 bases:

>100_bases
TGGCGCGCCGCGGTGCGAGCCCCGTGATCAATGCCATCTCGGCCTTGATCACACTGGTCTTCGGAGCGCTTATCCTGGTT
TCAGAGCGGATGCGGGAAGC

Downstream 100 bases:

>100_bases
CCAACGAGCCGCGACCGTTAGGGAGCGCTACCCGCACACCACCCCGTCGTACGCGCCCCTTACGGTCGCGCCTCATCGGC
ACTTTGTCTCCACCGCGAAC

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MNRRYFIFGLAGLAGCARDARPRLNVYNWSSYIAPETVPAFEAEFGVRVRYATYESNEEMLAKVITGNSGWDIVVPTHSR
IEPMRGAGLLAALDHAALPGLANLDPRFRAPVWDPALQWCVPYMWNGTGVVYNKSLRPAPQRWADLWSPQLKGRLTMLDD
PEDMLGACLKKLSLSFNATDPAQLQRAEHEAIAQKPLLRAYLNAEVRDQLVAGDVLAAQLWSTTAQQAIDAAPQLAFAYP
AEGFPFYCDCAVILRESRRPELAHKFLNFLLRPPVSAQIVQATRTATANGAALSLLPEDTRNNPALYPGPEIYARGEWPH
PVPNATQRLRDRIWTEIKSA

Sequences:

>Translated_340_residues
MNRRYFIFGLAGLAGCARDARPRLNVYNWSSYIAPETVPAFEAEFGVRVRYATYESNEEMLAKVITGNSGWDIVVPTHSR
IEPMRGAGLLAALDHAALPGLANLDPRFRAPVWDPALQWCVPYMWNGTGVVYNKSLRPAPQRWADLWSPQLKGRLTMLDD
PEDMLGACLKKLSLSFNATDPAQLQRAEHEAIAQKPLLRAYLNAEVRDQLVAGDVLAAQLWSTTAQQAIDAAPQLAFAYP
AEGFPFYCDCAVILRESRRPELAHKFLNFLLRPPVSAQIVQATRTATANGAALSLLPEDTRNNPALYPGPEIYARGEWPH
PVPNATQRLRDRIWTEIKSA
>Mature_340_residues
MNRRYFIFGLAGLAGCARDARPRLNVYNWSSYIAPETVPAFEAEFGVRVRYATYESNEEMLAKVITGNSGWDIVVPTHSR
IEPMRGAGLLAALDHAALPGLANLDPRFRAPVWDPALQWCVPYMWNGTGVVYNKSLRPAPQRWADLWSPQLKGRLTMLDD
PEDMLGACLKKLSLSFNATDPAQLQRAEHEAIAQKPLLRAYLNAEVRDQLVAGDVLAAQLWSTTAQQAIDAAPQLAFAYP
AEGFPFYCDCAVILRESRRPELAHKFLNFLLRPPVSAQIVQATRTATANGAALSLLPEDTRNNPALYPGPEIYARGEWPH
PVPNATQRLRDRIWTEIKSA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=331, Percent_Identity=32.3262839879154, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1787078, Length=352, Percent_Identity=30.9659090909091, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1787711, Length=311, Percent_Identity=24.4372990353698, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37864; Mature: 37864

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRYFIFGLAGLAGCARDARPRLNVYNWSSYIAPETVPAFEAEFGVRVRYATYESNEEM
CCCCEEEEECCHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHH
LAKVITGNSGWDIVVPTHSRIEPMRGAGLLAALDHAALPGLANLDPRFRAPVWDPALQWC
HHHHHCCCCCCEEEECCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
VPYMWNGTGVVYNKSLRPAPQRWADLWSPQLKGRLTMLDDPEDMLGACLKKLSLSFNATD
HHHEECCCEEEEECCCCCCHHHHHHHCCHHHCCEEEEECCHHHHHHHHHHHHCCCCCCCC
PAQLQRAEHEAIAQKPLLRAYLNAEVRDQLVAGDVLAAQLWSTTAQQAIDAAPQLAFAYP
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
AEGFPFYCDCAVILRESRRPELAHKFLNFLLRPPVSAQIVQATRTATANGAALSLLPEDT
CCCCCCEEHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEECCCCC
RNNPALYPGPEIYARGEWPHPVPNATQRLRDRIWTEIKSA
CCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRRYFIFGLAGLAGCARDARPRLNVYNWSSYIAPETVPAFEAEFGVRVRYATYESNEEM
CCCCEEEEECCHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHH
LAKVITGNSGWDIVVPTHSRIEPMRGAGLLAALDHAALPGLANLDPRFRAPVWDPALQWC
HHHHHCCCCCCEEEECCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
VPYMWNGTGVVYNKSLRPAPQRWADLWSPQLKGRLTMLDDPEDMLGACLKKLSLSFNATD
HHHEECCCEEEEECCCCCCHHHHHHHCCHHHCCEEEEECCHHHHHHHHHHHHCCCCCCCC
PAQLQRAEHEAIAQKPLLRAYLNAEVRDQLVAGDVLAAQLWSTTAQQAIDAAPQLAFAYP
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
AEGFPFYCDCAVILRESRRPELAHKFLNFLLRPPVSAQIVQATRTATANGAALSLLPEDT
CCCCCCEEHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEECCCCC
RNNPALYPGPEIYARGEWPHPVPNATQRLRDRIWTEIKSA
CCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]