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The map label for this gene is potB [H]

Identifier: 116621486

GI number: 116621486

Start: 3018432

End: 3019259

Strand: Direct

Name: potB [H]

Synonym: Acid_2368

Alternate gene names: 116621486

Gene position: 3018432-3019259 (Clockwise)

Preceding gene: 116621485

Following gene: 116621487

Centisome position: 30.29

GC content: 65.46

Gene sequence:

>828_bases
ATGAAGCTGCGCTCCTGGTTTCTGCTGCCCACTTGGATCTGGATGTGGCTGCTCTTCGCCGCGCCCTTCGCGATCGTGCT
GGCCTACAGCGCGTTGAGCCGCGGCGAGATCGGCGGGATCGAACCGCCGTGGACGCTCGAAAGCTACACCCGGCTGGCCG
ACCCGCTCTATCTGACGATTCTCTGGCGCTCCTTCGTGATGGCCGCCGCCGCCACCGCGCTGTGCCTGGTCTTCGCCTTT
CCCGCCGCGCTGTTCATTTCGCGCGCCACCTCGCGGCGCAATCTCTATCTGCAACTCGTCATGCTGCCCTTCTGGACCAG
CTTCCTGGTGCGCACCTACGCCTGGCTTTTCCTGCTGCGCGATACCGGCCTGGTCAACACGATCCTTCAGGCGCTCGGCC
TGATTCACGCTCCGCTGCCGCTGCTTTATAACGATGGCGCGGTGCTGCTCGGCCTGGTCTACGGTTATCTGCCCTTTATG
GTGCTGCCGGTCTACGCCACGCTCGAACGGCTCGACCCCTCGCTGGTGGAGGCCGCCGCCGACCTGGGCGCGCGTCCGCT
CACCACCGTCTTTCGCGTGATCGTGCCGCTCTCCAAACCCGGCATCGTCGCCGGCTCGATCCTGGTCTTCATCCCCTGTC
TGGGCGCCTACCTGACTCCCGACCTGCTCGGCGGAGGCCGCACCGTGATGGTCGGCAACCTGGTGCAGAACCAGTTCACC
ACCGCGCGCGACTGGCCGTTCGGTTCCGCGATTTCGATCGCGCTCATGGCCATCGTCACGCTGCTGCTGGTGATCTTCCT
GCGGCGCGAAAGCGAGTCGCTGGTATGA

Upstream 100 bases:

>100_bases
GCGAAAATGCCATCGTGCAACTGCCGCGCTGCGACGCCGGTTTCCAGCCCGGCGACTCCGTCCACTTGGCGTGGAACGCG
GCCGACGAGATGTCCCTGCC

Downstream 100 bases:

>100_bases
AGCGCTGGCTCGGAGTGTACGCGATCGCCTTCTTCGCCTTTCTGCACCTGCCGCTGTTGACGCTCGCCCTCTTCAGTTTC
AATTCCTCGAAGTTCACCAG

Product: binding-protein-dependent transporter inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKLRSWFLLPTWIWMWLLFAAPFAIVLAYSALSRGEIGGIEPPWTLESYTRLADPLYLTILWRSFVMAAAATALCLVFAF
PAALFISRATSRRNLYLQLVMLPFWTSFLVRTYAWLFLLRDTGLVNTILQALGLIHAPLPLLYNDGAVLLGLVYGYLPFM
VLPVYATLERLDPSLVEAAADLGARPLTTVFRVIVPLSKPGIVAGSILVFIPCLGAYLTPDLLGGGRTVMVGNLVQNQFT
TARDWPFGSAISIALMAIVTLLLVIFLRRESESLV

Sequences:

>Translated_275_residues
MKLRSWFLLPTWIWMWLLFAAPFAIVLAYSALSRGEIGGIEPPWTLESYTRLADPLYLTILWRSFVMAAAATALCLVFAF
PAALFISRATSRRNLYLQLVMLPFWTSFLVRTYAWLFLLRDTGLVNTILQALGLIHAPLPLLYNDGAVLLGLVYGYLPFM
VLPVYATLERLDPSLVEAAADLGARPLTTVFRVIVPLSKPGIVAGSILVFIPCLGAYLTPDLLGGGRTVMVGNLVQNQFT
TARDWPFGSAISIALMAIVTLLLVIFLRRESESLV
>Mature_275_residues
MKLRSWFLLPTWIWMWLLFAAPFAIVLAYSALSRGEIGGIEPPWTLESYTRLADPLYLTILWRSFVMAAAATALCLVFAF
PAALFISRATSRRNLYLQLVMLPFWTSFLVRTYAWLFLLRDTGLVNTILQALGLIHAPLPLLYNDGAVLLGLVYGYLPFM
VLPVYATLERLDPSLVEAAADLGARPLTTVFRVIVPLSKPGIVAGSILVFIPCLGAYLTPDLLGGGRTVMVGNLVQNQFT
TARDWPFGSAISIALMAIVTLLLVIFLRRESESLV

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=256, Percent_Identity=41.796875, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI1787080, Length=222, Percent_Identity=46.8468468468468, Blast_Score=207, Evalue=5e-55,
Organism=Escherichia coli, GI1787713, Length=243, Percent_Identity=38.2716049382716, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30456; Mature: 30456

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]