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The map label for this gene is ispF

Identifier: 116620980

GI number: 116620980

Start: 2315938

End: 2316417

Strand: Reverse

Name: ispF

Synonym: Acid_1861

Alternate gene names: 116620980

Gene position: 2316417-2315938 (Counterclockwise)

Preceding gene: 116620981

Following gene: 116620979

Centisome position: 23.24

GC content: 62.29

Gene sequence:

>480_bases
TTGAGCGAGATTCGAACCGGACTGGGCTGGGATGTTCACCGTATCGCGACCGGGCGGAAGATGATTCTGGGCGGGGTGAC
GGTGCCCTGCGAATTCGGGTTGGAAGGCCACTCCGACGCCGACGTGCTGGCGCATGCCATTACAGACGCAATTTTGGGCG
CGGCGGCGCTGGGCGATATCGGCATGCATTTTCCGGATTCGGACCCGCGCTGGAAGGGCTGCGACAGCCTGGTCTTTCTG
CGCCATGCGCGCGACCTGGCAACGCAGAAGGGCTATCGCATCGTCAACGTGGATTCGACGATCATTTTGGAACGCCCCAA
GTTGAAGGACTTCCGGCAGACGATCCGCGAGCGGCTGGCGGAAACTCTGGAGCTGGACGTGGACCGGGTCTCGGTGAAAT
TCAAGACCGCGGAAAAGGTAGGCCCGGTGGGCGAGGGCCGCTCGGCGGAATCGCAGGCGATCGTGACACTCGAGAAATAG

Upstream 100 bases:

>100_bases
TGGTGCCGGGCAGTGACCGCAATATCAAGATCACCAAGCCCACCGATATGGAGCTGGCGCATCTGTTCCTGCGCGAAGAA
GCCGCGCGGAGCACGCTCTC

Downstream 100 bases:

>100_bases
GTCTGGATCGCACGGAACCCGCCGCCGCCCGTGATGTACAATCCGTCATCGGAGTTCGCGTGAAGCGGCGAATTTTCATT
GGATCATCCACCGAGGGGCT

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MSEIRTGLGWDVHRIATGRKMILGGVTVPCEFGLEGHSDADVLAHAITDAILGAAALGDIGMHFPDSDPRWKGCDSLVFL
RHARDLATQKGYRIVNVDSTIILERPKLKDFRQTIRERLAETLELDVDRVSVKFKTAEKVGPVGEGRSAESQAIVTLEK

Sequences:

>Translated_159_residues
MSEIRTGLGWDVHRIATGRKMILGGVTVPCEFGLEGHSDADVLAHAITDAILGAAALGDIGMHFPDSDPRWKGCDSLVFL
RHARDLATQKGYRIVNVDSTIILERPKLKDFRQTIRERLAETLELDVDRVSVKFKTAEKVGPVGEGRSAESQAIVTLEK
>Mature_158_residues
SEIRTGLGWDVHRIATGRKMILGGVTVPCEFGLEGHSDADVLAHAITDAILGAAALGDIGMHFPDSDPRWKGCDSLVFLR
HARDLATQKGYRIVNVDSTIILERPKLKDFRQTIRERLAETLELDVDRVSVKFKTAEKVGPVGEGRSAESQAIVTLEK

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=47.4358974358974, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_SOLUE (Q027G2)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_823136.1
- ProteinModelPortal:   Q027G2
- SMR:   Q027G2
- STRING:   Q027G2
- GeneID:   4429972
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_1861
- NMPDR:   fig|234267.9.peg.1774
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   Q027G2
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17449; Mature: 17318

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIRTGLGWDVHRIATGRKMILGGVTVPCEFGLEGHSDADVLAHAITDAILGAAALGDI
CCCCCCCCCCCHHHHHCCCEEEECCEECCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GMHFPDSDPRWKGCDSLVFLRHARDLATQKGYRIVNVDSTIILERPKLKDFRQTIRERLA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCHHHHHHHHHHHH
ETLELDVDRVSVKFKTAEKVGPVGEGRSAESQAIVTLEK
HHHHCCCHHEEEEEECHHHCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
SEIRTGLGWDVHRIATGRKMILGGVTVPCEFGLEGHSDADVLAHAITDAILGAAALGDI
CCCCCCCCCCHHHHHCCCEEEECCEECCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHC
GMHFPDSDPRWKGCDSLVFLRHARDLATQKGYRIVNVDSTIILERPKLKDFRQTIRERLA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCHHHHHHHHHHHH
ETLELDVDRVSVKFKTAEKVGPVGEGRSAESQAIVTLEK
HHHHCCCHHEEEEEECHHHCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA