Click here to switch to the map view.

The map label for this gene is ispD

Identifier: 116620981

GI number: 116620981

Start: 2316414

End: 2317148

Strand: Reverse

Name: ispD

Synonym: Acid_1862

Alternate gene names: 116620981

Gene position: 2317148-2316414 (Counterclockwise)

Preceding gene: 116620982

Following gene: 116620980

Centisome position: 23.25

GC content: 65.71

Gene sequence:

>735_bases
ATGAAAGTCGCCGTGATATTACCCGCGGCCGGCCTGGGCACCCGTATGGGAAAAGGCACCGCGGAGAAGGCCGGCACCAG
CCGCAAGCAATTCATGCTGCTGGAGGGCTCGCCGATTCTGATGCACACCGTGCGCAAGTTTGCCGCCTCTCCCCGCGTGG
GGGAGATCGTGATCGCGGTGCGCGCCGAAGATACCGAGTGGGTTCGAGAAGTGGTGACGCAGGAATTTCCAGGCGGCCGG
GTGCGCGTGGTCGCGGGCGGCAACAGCCGGCAGGAATCGGTGGAGAACGCGCTGAGCTCGCTGAGTCCGGATTGCGAACT
GGTGGCAGTGCACGACGCGGTGCGCCCGTTTATCGACCTGGACATCATGCACGCGGTCTTCGACGAGGCCGCGGAAACCG
GCGCGGCGATTGTCGGCGTCCCGGCGGTGGACACCGTGAAGCAGGTGACGCGGGGCACCACCCACGTGCGGGTGCGGGCG
ACCCTGCCGCGCGAAAAGCTGGTGATGGCGCAGACCCCGCAGGTCTTCCGCCACGATTTGCTGCTGCGCGCGTTTCAGGA
AGCGCGCAAGGACGGCTTCATCGGCACCGACGAAAGCAGCATGGTGGAGCGGCTCGACGTGGAAGTCAGCGTGGTGCCGG
GCAGTGACCGCAATATCAAGATCACCAAGCCCACCGATATGGAGCTGGCGCATCTGTTCCTGCGCGAAGAAGCCGCGCGG
AGCACGCTCTCTTGA

Upstream 100 bases:

>100_bases
GCGGTAGGCGCCGAGCGGCACGACCGTCCGGTGCGCAAGAGCGATCCGCAGCCGATGACGAATATGCCGCCCGAGAAAAC
CACGATAGAATCGTAAGTAC

Downstream 100 bases:

>100_bases
GCGAGATTCGAACCGGACTGGGCTGGGATGTTCACCGTATCGCGACCGGGCGGAAGATGATTCTGGGCGGGGTGACGGTG
CCCTGCGAATTCGGGTTGGA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MKVAVILPAAGLGTRMGKGTAEKAGTSRKQFMLLEGSPILMHTVRKFAASPRVGEIVIAVRAEDTEWVREVVTQEFPGGR
VRVVAGGNSRQESVENALSSLSPDCELVAVHDAVRPFIDLDIMHAVFDEAAETGAAIVGVPAVDTVKQVTRGTTHVRVRA
TLPREKLVMAQTPQVFRHDLLLRAFQEARKDGFIGTDESSMVERLDVEVSVVPGSDRNIKITKPTDMELAHLFLREEAAR
STLS

Sequences:

>Translated_244_residues
MKVAVILPAAGLGTRMGKGTAEKAGTSRKQFMLLEGSPILMHTVRKFAASPRVGEIVIAVRAEDTEWVREVVTQEFPGGR
VRVVAGGNSRQESVENALSSLSPDCELVAVHDAVRPFIDLDIMHAVFDEAAETGAAIVGVPAVDTVKQVTRGTTHVRVRA
TLPREKLVMAQTPQVFRHDLLLRAFQEARKDGFIGTDESSMVERLDVEVSVVPGSDRNIKITKPTDMELAHLFLREEAAR
STLS
>Mature_244_residues
MKVAVILPAAGLGTRMGKGTAEKAGTSRKQFMLLEGSPILMHTVRKFAASPRVGEIVIAVRAEDTEWVREVVTQEFPGGR
VRVVAGGNSRQESVENALSSLSPDCELVAVHDAVRPFIDLDIMHAVFDEAAETGAAIVGVPAVDTVKQVTRGTTHVRVRA
TLPREKLVMAQTPQVFRHDLLLRAFQEARKDGFIGTDESSMVERLDVEVSVVPGSDRNIKITKPTDMELAHLFLREEAAR
STLS

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=244, Percent_Identity=28.6885245901639, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI157671913, Length=142, Percent_Identity=31.6901408450704, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1789104, Length=236, Percent_Identity=32.2033898305085, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_SOLUE (Q027G1)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_823137.1
- ProteinModelPortal:   Q027G1
- SMR:   Q027G1
- STRING:   Q027G1
- GeneID:   4429973
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_1862
- NMPDR:   fig|234267.9.peg.1775
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   PSNIKVT
- PhylomeDB:   Q027G1
- ProtClustDB:   CLSK774228
- HAMAP:   MF_00108
- InterPro:   IPR001228
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 26563; Mature: 26563

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVILPAAGLGTRMGKGTAEKAGTSRKQFMLLEGSPILMHTVRKFAASPRVGEIVIAV
CEEEEEECCCCCCCCCCCCCHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEEEE
RAEDTEWVREVVTQEFPGGRVRVVAGGNSRQESVENALSSLSPDCELVAVHDAVRPFIDL
ECCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHH
DIMHAVFDEAAETGAAIVGVPAVDTVKQVTRGTTHVRVRATLPREKLVMAQTPQVFRHDL
HHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCEEEEEEECCHHHEEECCCHHHHHHHH
LLRAFQEARKDGFIGTDESSMVERLDVEVSVVPGSDRNIKITKPTDMELAHLFLREEAAR
HHHHHHHHHHCCCCCCCHHHHHHHHCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHH
STLS
HCCC
>Mature Secondary Structure
MKVAVILPAAGLGTRMGKGTAEKAGTSRKQFMLLEGSPILMHTVRKFAASPRVGEIVIAV
CEEEEEECCCCCCCCCCCCCHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEEEE
RAEDTEWVREVVTQEFPGGRVRVVAGGNSRQESVENALSSLSPDCELVAVHDAVRPFIDL
ECCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHH
DIMHAVFDEAAETGAAIVGVPAVDTVKQVTRGTTHVRVRATLPREKLVMAQTPQVFRHDL
HHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCEEEEEEECCHHHEEECCCHHHHHHHH
LLRAFQEARKDGFIGTDESSMVERLDVEVSVVPGSDRNIKITKPTDMELAHLFLREEAAR
HHHHHHHHHHCCCCCCCHHHHHHHHCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHH
STLS
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA