Click here to switch to the map view.

The map label for this gene is pemA [H]

Identifier: 116620372

GI number: 116620372

Start: 1545998

End: 1547551

Strand: Reverse

Name: pemA [H]

Synonym: Acid_1249

Alternate gene names: 116620372

Gene position: 1547551-1545998 (Counterclockwise)

Preceding gene: 116620376

Following gene: 116620365

Centisome position: 15.53

GC content: 63.9

Gene sequence:

>1554_bases
GTGCGCGCCGCCGATCGCGAATTCGTTGTCGCCCCCGATGGTCCCTACAAAACTGTGCAGTCCGCCGTGGATGCCGCGCC
GCCTCACTCAATGATTCACATCCGACCCGGGATCTACAAAGAGCGCGTCGTCGTTCCGTATCAGAAGCCCCATATCACCT
TCCGAGGGGACGACGCTCAAACCACCATCATCACTTTCGATGCGCACACCGGGCAGCCGGGACCCAAAGGCCCGATCAAC
ACCTTCGCCACTCCAACGGTCTTCATTCAGGCGAACGATTTCACGGCAGAGAACCTGACCTTCGCCAACTCCGCCGGCAA
TGTCGGACAGGCTGTCGCGCTGACCATCATGGGCGACCGCGGCGTCTTCCGCCATTGCCGTTTCCTCGGATATCAGGACA
CGCTGCTGCCGCAGGCCGGCCGCCAGTATTTCGACCACTGCTACATAGAAGGCGCCACCGATTTCATCTTCGGAGGCTCG
GCCGCCTGGTTCGACCGCTGCGCGATCCACGCGACCGCCAACGGCTATCTGACCGCCGCTAACACCACCAAAGATCAAGC
CTACGGCTACGTTTTCGACCACGCCACGATCACCGGCGCGCCCAAGGTCAAAACCTACCTCGGCCGCCCCTGGAGACCCT
GGGCCGCCACCGTCTTCCTCAACAGTGAAATCTCCGACGTGCTCCGCCCCGAGGGTTGGAACAACTGGAACGACCCCACC
CGCGAGCAGACCGTCCGCTACGCCGAATACCCCGCGCCCGCAGGCCGCGTGCCGTGGGCCAAAGCGCTCACCGCCGCCGA
AGCCGCGCAATACACCATCGAAAAGGTGCTCTCCGGCCTGGATGGCTGGAACGCCAGGACCGGCGCGGTCCGCAAGTCCG
TCAAGGTCGTCGAGGCCAAAGCCGGGAAGGCCCCGCCTGTCCCGCGTTATCGCAACGTGATCGCCGGGCCGGAAGGTCTC
CACCACGCCGTCTGGGCCGATGGCAAGCAACTCGCTCACGCATCATCGCGCGACCTGCAGACCTGGTCGGCGTCCCAAGC
CACCGACATCATGGCCGGCAAGAACGCGCTCGATCTCGAATCGCCGAACCTCTTCTACGACGATGCGGCCGGGCAGTTCA
TCGTCACCTGGTCCTGCACGCTCGCGCGCAATGCCATCCAGGCCTTCCAGGAAGACGTCGATCACAATCCGCGCATCTGG
TACGCCACCACGAAGGATTTCACCGCATACTCCGAGCCCAAGCTTTTGTTCGATAACAACTACGCCGTCCGCGACGCCCA
GATTCTCAAGCTCGCGTCCGGCTACGCGCTCCTGCACACGGACAGCACCTGGCCGATGCAGAATCTCCGGGTTGCCTTCT
CCGCCACCCCTCTCGGCCCGTGGGGTCCGTCTACCGACGCCTTCACCGCCAAGGGCACCCATTCGCCTGTCGCTATTCAA
TCCGCCGGCGAGTGGTGGATCTTCCATTCGGGCGGCCTCGTCAAAACCCGCGACTTCTGGAGCTACGAGGAACTCCCCGG
CGCCTTCCCGCTGCCGCCGTTATTCACCCGGTGA

Upstream 100 bases:

>100_bases
GCGGCCAGGAGGCCGACCAGCGCAACCCGGCAAGTCGATTTCATGCGTCTATAATAACGGGCAGATGAAGGTCATTGCGG
TCTTATTATTTGCCATTACG

Downstream 100 bases:

>100_bases
TTGCAGGGTGTTGTTGGCCAGATCGCGCCATGCGTCGCTGCCGCCCAGCAAGGGCCGCACCCGGACAATCCCGTGACTCT
TGAGGAGGAGCGCCGCACGG

Product: pectinesterase

Products: NA

Alternate protein names: PE A; Pectin methylesterase A [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MRAADREFVVAPDGPYKTVQSAVDAAPPHSMIHIRPGIYKERVVVPYQKPHITFRGDDAQTTIITFDAHTGQPGPKGPIN
TFATPTVFIQANDFTAENLTFANSAGNVGQAVALTIMGDRGVFRHCRFLGYQDTLLPQAGRQYFDHCYIEGATDFIFGGS
AAWFDRCAIHATANGYLTAANTTKDQAYGYVFDHATITGAPKVKTYLGRPWRPWAATVFLNSEISDVLRPEGWNNWNDPT
REQTVRYAEYPAPAGRVPWAKALTAAEAAQYTIEKVLSGLDGWNARTGAVRKSVKVVEAKAGKAPPVPRYRNVIAGPEGL
HHAVWADGKQLAHASSRDLQTWSASQATDIMAGKNALDLESPNLFYDDAAGQFIVTWSCTLARNAIQAFQEDVDHNPRIW
YATTKDFTAYSEPKLLFDNNYAVRDAQILKLASGYALLHTDSTWPMQNLRVAFSATPLGPWGPSTDAFTAKGTHSPVAIQ
SAGEWWIFHSGGLVKTRDFWSYEELPGAFPLPPLFTR

Sequences:

>Translated_517_residues
MRAADREFVVAPDGPYKTVQSAVDAAPPHSMIHIRPGIYKERVVVPYQKPHITFRGDDAQTTIITFDAHTGQPGPKGPIN
TFATPTVFIQANDFTAENLTFANSAGNVGQAVALTIMGDRGVFRHCRFLGYQDTLLPQAGRQYFDHCYIEGATDFIFGGS
AAWFDRCAIHATANGYLTAANTTKDQAYGYVFDHATITGAPKVKTYLGRPWRPWAATVFLNSEISDVLRPEGWNNWNDPT
REQTVRYAEYPAPAGRVPWAKALTAAEAAQYTIEKVLSGLDGWNARTGAVRKSVKVVEAKAGKAPPVPRYRNVIAGPEGL
HHAVWADGKQLAHASSRDLQTWSASQATDIMAGKNALDLESPNLFYDDAAGQFIVTWSCTLARNAIQAFQEDVDHNPRIW
YATTKDFTAYSEPKLLFDNNYAVRDAQILKLASGYALLHTDSTWPMQNLRVAFSATPLGPWGPSTDAFTAKGTHSPVAIQ
SAGEWWIFHSGGLVKTRDFWSYEELPGAFPLPPLFTR
>Mature_517_residues
MRAADREFVVAPDGPYKTVQSAVDAAPPHSMIHIRPGIYKERVVVPYQKPHITFRGDDAQTTIITFDAHTGQPGPKGPIN
TFATPTVFIQANDFTAENLTFANSAGNVGQAVALTIMGDRGVFRHCRFLGYQDTLLPQAGRQYFDHCYIEGATDFIFGGS
AAWFDRCAIHATANGYLTAANTTKDQAYGYVFDHATITGAPKVKTYLGRPWRPWAATVFLNSEISDVLRPEGWNNWNDPT
REQTVRYAEYPAPAGRVPWAKALTAAEAAQYTIEKVLSGLDGWNARTGAVRKSVKVVEAKAGKAPPVPRYRNVIAGPEGL
HHAVWADGKQLAHASSRDLQTWSASQATDIMAGKNALDLESPNLFYDDAAGQFIVTWSCTLARNAIQAFQEDVDHNPRIW
YATTKDFTAYSEPKLLFDNNYAVRDAQILKLASGYALLHTDSTWPMQNLRVAFSATPLGPWGPSTDAFTAKGTHSPVAIQ
SAGEWWIFHSGGLVKTRDFWSYEELPGAFPLPPLFTR

Specific function: Involved in maceration and soft-rotting of plant tissue [H]

COG id: COG4677

COG function: function code G; Pectin methylesterase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pectinesterase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012334
- InterPro:   IPR011050
- InterPro:   IPR018040
- InterPro:   IPR000070 [H]

Pfam domain/function: PF01095 Pectinesterase [H]

EC number: =3.1.1.11 [H]

Molecular weight: Translated: 56931; Mature: 56931

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00503 PECTINESTERASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAADREFVVAPDGPYKTVQSAVDAAPPHSMIHIRPGIYKERVVVPYQKPHITFRGDDAQ
CCCCCCCEEEECCCCHHHHHHHHHCCCCCCEEEECCCCCCCCEEECCCCCEEEEECCCCE
TTIITFDAHTGQPGPKGPINTFATPTVFIQANDFTAENLTFANSAGNVGQAVALTIMGDR
EEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCEEEEEEECCC
GVFRHCRFLGYQDTLLPQAGRQYFDHCYIEGATDFIFGGSAAWFDRCAIHATANGYLTAA
CHHHHHHHCCCCHHHCCHHHHHHHHEEEECCCCEEEECCCHHHHHCEEEEEECCCEEEEC
NTTKDQAYGYVFDHATITGAPKVKTYLGRPWRPWAATVFLNSEISDVLRPEGWNNWNDPT
CCCCCCCCEEEEECEEECCCCCHHHHCCCCCCCEEEEEEECCCHHHHHCCCCCCCCCCCC
REQTVRYAEYPAPAGRVPWAKALTAAEAAQYTIEKVLSGLDGWNARTGAVRKSVKVVEAK
HHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEEEEC
AGKAPPVPRYRNVIAGPEGLHHAVWADGKQLAHASSRDLQTWSASQATDIMAGKNALDLE
CCCCCCCCHHHHHCCCCCCCCHHHCCCCHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCC
SPNLFYDDAAGQFIVTWSCTLARNAIQAFQEDVDHNPRIWYATTKDFTAYSEPKLLFDNN
CCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEECCC
YAVRDAQILKLASGYALLHTDSTWPMQNLRVAFSATPLGPWGPSTDAFTAKGTHSPVAIQ
EEECCCEEEEEECCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCEEECCCCCCEEEE
SAGEWWIFHSGGLVKTRDFWSYEELPGAFPLPPLFTR
CCCCEEEEECCCEEEECCCCCHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRAADREFVVAPDGPYKTVQSAVDAAPPHSMIHIRPGIYKERVVVPYQKPHITFRGDDAQ
CCCCCCCEEEECCCCHHHHHHHHHCCCCCCEEEECCCCCCCCEEECCCCCEEEEECCCCE
TTIITFDAHTGQPGPKGPINTFATPTVFIQANDFTAENLTFANSAGNVGQAVALTIMGDR
EEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCEEEEEEECCC
GVFRHCRFLGYQDTLLPQAGRQYFDHCYIEGATDFIFGGSAAWFDRCAIHATANGYLTAA
CHHHHHHHCCCCHHHCCHHHHHHHHEEEECCCCEEEECCCHHHHHCEEEEEECCCEEEEC
NTTKDQAYGYVFDHATITGAPKVKTYLGRPWRPWAATVFLNSEISDVLRPEGWNNWNDPT
CCCCCCCCEEEEECEEECCCCCHHHHCCCCCCCEEEEEEECCCHHHHHCCCCCCCCCCCC
REQTVRYAEYPAPAGRVPWAKALTAAEAAQYTIEKVLSGLDGWNARTGAVRKSVKVVEAK
HHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEEEEC
AGKAPPVPRYRNVIAGPEGLHHAVWADGKQLAHASSRDLQTWSASQATDIMAGKNALDLE
CCCCCCCCHHHHHCCCCCCCCHHHCCCCHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCC
SPNLFYDDAAGQFIVTWSCTLARNAIQAFQEDVDHNPRIWYATTKDFTAYSEPKLLFDNN
CCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEECCC
YAVRDAQILKLASGYALLHTDSTWPMQNLRVAFSATPLGPWGPSTDAFTAKGTHSPVAIQ
EEECCCEEEEEECCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCEEECCCCCCEEEE
SAGEWWIFHSGGLVKTRDFWSYEELPGAFPLPPLFTR
CCCCEEEEECCCEEEECCCCCHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8370537 [H]