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The map label for this gene is purR [H]

Identifier: 116620357

GI number: 116620357

Start: 1525819

End: 1526817

Strand: Reverse

Name: purR [H]

Synonym: Acid_1234

Alternate gene names: 116620357

Gene position: 1526817-1525819 (Counterclockwise)

Preceding gene: 116620365

Following gene: 116620356

Centisome position: 15.32

GC content: 62.06

Gene sequence:

>999_bases
ATGAACCTGGAACAGGTGGCCCGCCGTGCCAAGGTATCTACCGCTACCGTCTCGCGCGTGCTTAACAATGCAAGCGTAGT
CAAGACTTCCACACGCGCCCGCGTGATGAAGGCCATCGAAGAATTGAAGTACCACCCTAACCTTCACGCGCGCAATCTGG
CGGGCGGCAAGAGCCGGACTCTCGGCGTCATCGTCTCCAACATGGAGAACCCGTTCTTCTTCGATATCTACAAAACCGTG
GAGAGCGACGCCCACGCGGCGGGCTACGAAGTCGTCGTCGCCAATACCGACTACCGCAGCGAGCAGCTCGTCTCCAGCAT
TCGCCTCATGATCGGCCGCCGCGTCGCCGGTCTCGCCGCGGTCGTCTCCGAAATGGAGCCCGCCCTGATGGACGAGCTCA
CCGACAGCAACATCCCCGTCGTCTTCTACGACGTGGGCGCGCCTCGCCAGAACATCACCAACATCCGCGTCAATTACCGC
CGCGGCATCGAAAAGATCGTTGACTACCTGTACAGCCTAGGCCACCGCAAACTCGGCTTCGTCGGCCACCACTCCACCCT
CGGCCCCATCACCGATCGTGTCAAGCCCCTGATGGATGCGGCCGCGCGCTACTCAGACCTCAGCGTCCGCGCCTCCGCCG
GTGAAGACACTCTGGATGGCGGCCGCCTCGCCGCGCGCAACCTGCTCGACTCCGGGGATCAGCCCAGCGCCATCGTCTGC
GTTAACGACATCATGGCCGTGGGCGCCCTCCGCGAACTCCGTGAGCGCGGATTGCGGATCCCGCAGGATATCTCCGTCAC
CGGATTCGACAACGTGAAGCTCTCCGAATTCTGCTACCCTGCTCTCACTACGGTTCATATTCCGCGGGACCGGATCGGAC
ATTTGATCTGCGAATCGTTACTGGCGAAATCCGAAAATACCGCGGCGGCCGATCACGAAATGGTGATCGACCCGGAGTTT
GTTTTGCGCGATTCCACGGGCCCCGCGCCCGTGCGCTGA

Upstream 100 bases:

>100_bases
CGTGCCTCATTCGTGTGCCTTCGTGGCCCCAAAGATTTTTCTTGTAACCGCTCTCACCAGGCAGTGCGGATCTTAGGATT
CCATATACAATGTTAGGGTC

Downstream 100 bases:

>100_bases
GACGCCTAGTGCGTCCTTGACACGAAAGTTGAGGCCCCATACTATATGCAGGTGCAAGCGATTACAGCATTCTCCCCGGC
GTTGCTGCTGCAAGCGCAGC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MNLEQVARRAKVSTATVSRVLNNASVVKTSTRARVMKAIEELKYHPNLHARNLAGGKSRTLGVIVSNMENPFFFDIYKTV
ESDAHAAGYEVVVANTDYRSEQLVSSIRLMIGRRVAGLAAVVSEMEPALMDELTDSNIPVVFYDVGAPRQNITNIRVNYR
RGIEKIVDYLYSLGHRKLGFVGHHSTLGPITDRVKPLMDAAARYSDLSVRASAGEDTLDGGRLAARNLLDSGDQPSAIVC
VNDIMAVGALRELRERGLRIPQDISVTGFDNVKLSEFCYPALTTVHIPRDRIGHLICESLLAKSENTAAADHEMVIDPEF
VLRDSTGPAPVR

Sequences:

>Translated_332_residues
MNLEQVARRAKVSTATVSRVLNNASVVKTSTRARVMKAIEELKYHPNLHARNLAGGKSRTLGVIVSNMENPFFFDIYKTV
ESDAHAAGYEVVVANTDYRSEQLVSSIRLMIGRRVAGLAAVVSEMEPALMDELTDSNIPVVFYDVGAPRQNITNIRVNYR
RGIEKIVDYLYSLGHRKLGFVGHHSTLGPITDRVKPLMDAAARYSDLSVRASAGEDTLDGGRLAARNLLDSGDQPSAIVC
VNDIMAVGALRELRERGLRIPQDISVTGFDNVKLSEFCYPALTTVHIPRDRIGHLICESLLAKSENTAAADHEMVIDPEF
VLRDSTGPAPVR
>Mature_332_residues
MNLEQVARRAKVSTATVSRVLNNASVVKTSTRARVMKAIEELKYHPNLHARNLAGGKSRTLGVIVSNMENPFFFDIYKTV
ESDAHAAGYEVVVANTDYRSEQLVSSIRLMIGRRVAGLAAVVSEMEPALMDELTDSNIPVVFYDVGAPRQNITNIRVNYR
RGIEKIVDYLYSLGHRKLGFVGHHSTLGPITDRVKPLMDAAARYSDLSVRASAGEDTLDGGRLAARNLLDSGDQPSAIVC
VNDIMAVGALRELRERGLRIPQDISVTGFDNVKLSEFCYPALTTVHIPRDRIGHLICESLLAKSENTAAADHEMVIDPEF
VLRDSTGPAPVR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=331, Percent_Identity=32.02416918429, Blast_Score=178, Evalue=3e-46,
Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=30.952380952381, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1790194, Length=328, Percent_Identity=29.2682926829268, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789202, Length=289, Percent_Identity=32.1799307958478, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788474, Length=301, Percent_Identity=31.2292358803987, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1789068, Length=302, Percent_Identity=29.4701986754967, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1786540, Length=349, Percent_Identity=29.512893982808, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI48994940, Length=306, Percent_Identity=27.7777777777778, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1787580, Length=309, Percent_Identity=27.1844660194175, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790715, Length=310, Percent_Identity=26.4516129032258, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789456, Length=308, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1787906, Length=321, Percent_Identity=23.98753894081, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786268, Length=318, Percent_Identity=22.3270440251572, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790689, Length=298, Percent_Identity=24.1610738255034, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36455; Mature: 36455

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEQVARRAKVSTATVSRVLNNASVVKTSTRARVMKAIEELKYHPNLHARNLAGGKSRT
CCHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCE
LGVIVSNMENPFFFDIYKTVESDAHAAGYEVVVANTDYRSEQLVSSIRLMIGRRVAGLAA
EEEEEECCCCCEEEHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
VVSEMEPALMDELTDSNIPVVFYDVGAPRQNITNIRVNYRRGIEKIVDYLYSLGHRKLGF
HHHHHCHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEE
VGHHSTLGPITDRVKPLMDAAARYSDLSVRASAGEDTLDGGRLAARNLLDSGDQPSAIVC
ECCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCEEEE
VNDIMAVGALRELRERGLRIPQDISVTGFDNVKLSEFCYPALTTVHIPRDRIGHLICESL
HHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHEEEECCHHHHHHHHHHHH
LAKSENTAAADHEMVIDPEFVLRDSTGPAPVR
HHCCCCCCCCCCCEEECCHHEEECCCCCCCCC
>Mature Secondary Structure
MNLEQVARRAKVSTATVSRVLNNASVVKTSTRARVMKAIEELKYHPNLHARNLAGGKSRT
CCHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCE
LGVIVSNMENPFFFDIYKTVESDAHAAGYEVVVANTDYRSEQLVSSIRLMIGRRVAGLAA
EEEEEECCCCCEEEHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
VVSEMEPALMDELTDSNIPVVFYDVGAPRQNITNIRVNYRRGIEKIVDYLYSLGHRKLGF
HHHHHCHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEE
VGHHSTLGPITDRVKPLMDAAARYSDLSVRASAGEDTLDGGRLAARNLLDSGDQPSAIVC
ECCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCEEEE
VNDIMAVGALRELRERGLRIPQDISVTGFDNVKLSEFCYPALTTVHIPRDRIGHLICESL
HHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHEEEECCHHHHHHHHHHHH
LAKSENTAAADHEMVIDPEFVLRDSTGPAPVR
HHCCCCCCCCCCCEEECCHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA