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The map label for this gene is nudF [H]

Identifier: 116619977

GI number: 116619977

Start: 1053008

End: 1053532

Strand: Direct

Name: nudF [H]

Synonym: Acid_0849

Alternate gene names: 116619977

Gene position: 1053008-1053532 (Clockwise)

Preceding gene: 116619976

Following gene: 116619979

Centisome position: 10.57

GC content: 58.29

Gene sequence:

>525_bases
ATGAAAATCACATCTTCCAAGCAGGTCTACGATTGCGGCCTCTTCCGCGTCACTGAGGATAGGGCGGTCGATCCGAAAAC
CAAGTTCGAGATCAAGCGCAGTGTGGTGCGCCACATCGGCAGTGCGGTGATGATGGCTATCGACGATAAGAATCGCGTGC
TGCTGGTGCGCCAATACCGTCTGCCAGCCGACAAGTACTTATGGGAATTGCCTGCCGGCCGGCTCGATGACGGCGAGAAG
CCGCTTGACGCGGCGAAGCGCGAGTTGAAAGAAGAGACGGGGTACGCGGCTAGGAAGTGGACCAAGCTGGCCAGTTACTA
CGCCAGCCCGGGCTTTGTGCAGGAGCGCATGACCATCTTCCTGGCAGAGGACTTGACCGCCGGCGAGGCCACTCCAATGG
ACGATGAGCGCATCGAGACGCGCTGGTTCAAGCGCAAGGAACTCGCCGAGATGATCGATGAAGGCAAAATTGAAGACGGC
AAGACCCTCATCGGCTTCCTTACCTGGCGCCGGAAGAAGAAGTAG

Upstream 100 bases:

>100_bases
GATATGGCGTGCTCACCGCGCGCCGCGGCTGGCTTGGCCCAGACGATATTTTGAATACGCGTAACGCCGGCGAGTTCGCC
AAGGCGCTCCAAACAAGATC

Downstream 100 bases:

>100_bases
GACCCAAGAGACCGCTGCCTAAGGGTCGCGGTTCGGTGACGGCTTCTTCCCTGGCAGGCCCACAGGGTTTTCGTGCGTGC
GCTCCTTGAAATTGCCGCGC

Product: NUDIX hydrolase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MKITSSKQVYDCGLFRVTEDRAVDPKTKFEIKRSVVRHIGSAVMMAIDDKNRVLLVRQYRLPADKYLWELPAGRLDDGEK
PLDAAKRELKEETGYAARKWTKLASYYASPGFVQERMTIFLAEDLTAGEATPMDDERIETRWFKRKELAEMIDEGKIEDG
KTLIGFLTWRRKKK

Sequences:

>Translated_174_residues
MKITSSKQVYDCGLFRVTEDRAVDPKTKFEIKRSVVRHIGSAVMMAIDDKNRVLLVRQYRLPADKYLWELPAGRLDDGEK
PLDAAKRELKEETGYAARKWTKLASYYASPGFVQERMTIFLAEDLTAGEATPMDDERIETRWFKRKELAEMIDEGKIEDG
KTLIGFLTWRRKKK
>Mature_174_residues
MKITSSKQVYDCGLFRVTEDRAVDPKTKFEIKRSVVRHIGSAVMMAIDDKNRVLLVRQYRLPADKYLWELPAGRLDDGEK
PLDAAKRELKEETGYAARKWTKLASYYASPGFVQERMTIFLAEDLTAGEATPMDDERIETRWFKRKELAEMIDEGKIEDG
KTLIGFLTWRRKKK

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=170, Percent_Identity=28.2352941176471, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1788810, Length=137, Percent_Identity=34.3065693430657, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 20225; Mature: 20225

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITSSKQVYDCGLFRVTEDRAVDPKTKFEIKRSVVRHIGSAVMMAIDDKNRVLLVRQYR
CCCCCCCCEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECC
LPADKYLWELPAGRLDDGEKPLDAAKRELKEETGYAARKWTKLASYYASPGFVQERMTIF
CCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHEEE
LAEDLTAGEATPMDDERIETRWFKRKELAEMIDEGKIEDGKTLIGFLTWRRKKK
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MKITSSKQVYDCGLFRVTEDRAVDPKTKFEIKRSVVRHIGSAVMMAIDDKNRVLLVRQYR
CCCCCCCCEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECC
LPADKYLWELPAGRLDDGEKPLDAAKRELKEETGYAARKWTKLASYYASPGFVQERMTIF
CCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHEEE
LAEDLTAGEATPMDDERIETRWFKRKELAEMIDEGKIEDGKTLIGFLTWRRKKK
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]