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The map label for this gene is dnaN [H]

Identifier: 116619147

GI number: 116619147

Start: 1637

End: 2755

Strand: Direct

Name: dnaN [H]

Synonym: Acid_0002

Alternate gene names: 116619147

Gene position: 1637-2755 (Clockwise)

Preceding gene: 116619146

Following gene: 116619148

Centisome position: 0.02

GC content: 60.77

Gene sequence:

>1119_bases
ATGGAATTCACCGTCAGCAAGAGCGATCTGGTACGTGAGCTGAGTCTCTCGCAGGGTGTGGTGGAAAAAAAGACCACCAT
TCCAATCCTGTCGAACGTGCTCCTGGAGGCCGCGGACGATCGCATTACGTTGACTGCGACCGACCTGGAACTGGGCATCC
GCTGTTCTTGCCCGGCGCGCGTCAAGAAAGAAGGGTCCGGCACGGTGCCGGCGCGCAAACTGCTCGATTACATGCGTCTG
TTGCCCGAAGGCGACGTGAACATGAAGTTCCTGGACAATCACTGGGCCAGTATCACCAGCGGACGTTCTCGCACCAGGAT
TGCGGGCATGTCGCGCGAGAGCTTCCCCGAACTGCCGCAGATGCCGGAGCCGATCGCGCAGGTGCCCGTCAAAACGCTGG
CCTCCATGATCGCCCGTACGTCCTTCGCCATCTCCATGGAGGAGTCGCGCTTTACGCTCAACGGCGCGCTCCTGCTGATG
CGCCCCGAAGGCCTCACCATGGTCGCCACCGATGGCCACCGCCTCGCCTACGTGCAGGCCGAGCCCGCGGAGAGCGGCAG
CGCCGACAAACCCTTCCGCGCCCTCGTCCCCAAGAAGGCGATGAGCGAATTGACCAAGCTCAGCGATGATGCCGGCGAAG
GCGCCATGGCCATCGTCGCCGGTGATGACAATCACCTGTTCTTCCAAATTGGCCACCGCCTGCTGATCACGCGAAAGCTC
ACCGGCAACTTCCCGGATTACGAGCGCGTCTTGCCCAAAGATCACCAGTTCATGGCCAAGCTCGAGAAGGGCGAAATCCG
CTCGGCAATCGAACGCGTGGCGCAGTTCGCCGATGAACGGTCGCGCGCCATTCGGGTGCAATTCAACAGCGGCGAAGTCC
GCGTCTTCAGTTCCAGCGTCGAAACCGGCGAGAGCGAAGAGAGCGTTCCCGCCGAGTACACGGGTCCCGATATCGAGATC
GGTTTCAATGCCCAATACCTGCTCGATTTCCTGCGTGCGATTTCTCAGGATCACGTTGCCTTCGAATTGAAGGATCAAAA
AAGCGCGGGCGAACTCCGTCCCGCCGGCGATGCCATCGCGGACCAGTACCGCTATGTAGTCATGCCGATGCGTATTTGA

Upstream 100 bases:

>100_bases
GTATAATTTAGATAGTCTTCAACACTTTGCACAGGGCCTATGAATACGGTTATTTATCAGGCCTATCTATAGAGACAGCA
AAGAACTAGGAGCCGTGGGC

Downstream 100 bases:

>100_bases
AGTTTAGTCAAACCAGTAAGGAACTATGCCAAACGAAGTCAATCCCGTCGGGGGAAATGGAAACGTCTACGATTCCAGCA
GTATCAAAGTCCTGGAAGGC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MEFTVSKSDLVRELSLSQGVVEKKTTIPILSNVLLEAADDRITLTATDLELGIRCSCPARVKKEGSGTVPARKLLDYMRL
LPEGDVNMKFLDNHWASITSGRSRTRIAGMSRESFPELPQMPEPIAQVPVKTLASMIARTSFAISMEESRFTLNGALLLM
RPEGLTMVATDGHRLAYVQAEPAESGSADKPFRALVPKKAMSELTKLSDDAGEGAMAIVAGDDNHLFFQIGHRLLITRKL
TGNFPDYERVLPKDHQFMAKLEKGEIRSAIERVAQFADERSRAIRVQFNSGEVRVFSSSVETGESEESVPAEYTGPDIEI
GFNAQYLLDFLRAISQDHVAFELKDQKSAGELRPAGDAIADQYRYVVMPMRI

Sequences:

>Translated_372_residues
MEFTVSKSDLVRELSLSQGVVEKKTTIPILSNVLLEAADDRITLTATDLELGIRCSCPARVKKEGSGTVPARKLLDYMRL
LPEGDVNMKFLDNHWASITSGRSRTRIAGMSRESFPELPQMPEPIAQVPVKTLASMIARTSFAISMEESRFTLNGALLLM
RPEGLTMVATDGHRLAYVQAEPAESGSADKPFRALVPKKAMSELTKLSDDAGEGAMAIVAGDDNHLFFQIGHRLLITRKL
TGNFPDYERVLPKDHQFMAKLEKGEIRSAIERVAQFADERSRAIRVQFNSGEVRVFSSSVETGESEESVPAEYTGPDIEI
GFNAQYLLDFLRAISQDHVAFELKDQKSAGELRPAGDAIADQYRYVVMPMRI
>Mature_372_residues
MEFTVSKSDLVRELSLSQGVVEKKTTIPILSNVLLEAADDRITLTATDLELGIRCSCPARVKKEGSGTVPARKLLDYMRL
LPEGDVNMKFLDNHWASITSGRSRTRIAGMSRESFPELPQMPEPIAQVPVKTLASMIARTSFAISMEESRFTLNGALLLM
RPEGLTMVATDGHRLAYVQAEPAESGSADKPFRALVPKKAMSELTKLSDDAGEGAMAIVAGDDNHLFFQIGHRLLITRKL
TGNFPDYERVLPKDHQFMAKLEKGEIRSAIERVAQFADERSRAIRVQFNSGEVRVFSSSVETGESEESVPAEYTGPDIEI
GFNAQYLLDFLRAISQDHVAFELKDQKSAGELRPAGDAIADQYRYVVMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=376, Percent_Identity=33.7765957446808, Blast_Score=192, Evalue=3e-50,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41199; Mature: 41199

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFTVSKSDLVRELSLSQGVVEKKTTIPILSNVLLEAADDRITLTATDLELGIRCSCPAR
CCEECCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHCCCCEEEEEEECEEECEEECCCHH
VKKEGSGTVPARKLLDYMRLLPEGDVNMKFLDNHWASITSGRSRTRIAGMSRESFPELPQ
HHCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCHHHHCCCCCCCEEECCCHHCCCCCCC
MPEPIAQVPVKTLASMIARTSFAISMEESRFTLNGALLLMRPEGLTMVATDGHRLAYVQA
CCCHHHHCCHHHHHHHHHHHHEEEEECCCEEEECCEEEEECCCCCEEEEECCCEEEEEEE
EPAESGSADKPFRALVPKKAMSELTKLSDDAGEGAMAIVAGDDNHLFFQIGHRLLITRKL
CCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCEEEEEEEC
TGNFPDYERVLPKDHQFMAKLEKGEIRSAIERVAQFADERSRAIRVQFNSGEVRVFSSSV
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCC
ETGESEESVPAEYTGPDIEIGFNAQYLLDFLRAISQDHVAFELKDQKSAGELRPAGDAIA
CCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHH
DQYRYVVMPMRI
CCCEEEEEEECC
>Mature Secondary Structure
MEFTVSKSDLVRELSLSQGVVEKKTTIPILSNVLLEAADDRITLTATDLELGIRCSCPAR
CCEECCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHCCCCEEEEEEECEEECEEECCCHH
VKKEGSGTVPARKLLDYMRLLPEGDVNMKFLDNHWASITSGRSRTRIAGMSRESFPELPQ
HHCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCHHHHCCCCCCCEEECCCHHCCCCCCC
MPEPIAQVPVKTLASMIARTSFAISMEESRFTLNGALLLMRPEGLTMVATDGHRLAYVQA
CCCHHHHCCHHHHHHHHHHHHEEEEECCCEEEECCEEEEECCCCCEEEEECCCEEEEEEE
EPAESGSADKPFRALVPKKAMSELTKLSDDAGEGAMAIVAGDDNHLFFQIGHRLLITRKL
CCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCEEEEEEEC
TGNFPDYERVLPKDHQFMAKLEKGEIRSAIERVAQFADERSRAIRVQFNSGEVRVFSSSV
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCC
ETGESEESVPAEYTGPDIEIGFNAQYLLDFLRAISQDHVAFELKDQKSAGELRPAGDAIA
CCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHH
DQYRYVVMPMRI
CCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9784136 [H]