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The map label for this gene is gyrB [H]

Identifier: 116619148

GI number: 116619148

Start: 2781

End: 5228

Strand: Direct

Name: gyrB [H]

Synonym: Acid_0003

Alternate gene names: 116619148

Gene position: 2781-5228 (Clockwise)

Preceding gene: 116619147

Following gene: 116619151

Centisome position: 0.03

GC content: 59.52

Gene sequence:

>2448_bases
ATGCCAAACGAAGTCAATCCCGTCGGGGGAAATGGAAACGTCTACGATTCCAGCAGTATCAAAGTCCTGGAAGGCCTCGA
AGCAGTGCGCCTGCGCCCTGCCATGTACATCGGGTCTACAGGCGAGATGGGGCTGCACCATCTGGTCTATGAGGTCGTCG
ATAATTCCGTCGATGAAGCTCTAGCCGGCTACGCCACCCATATCGAGGTGACGATCCACGACGATAATTCCGTCACCGTG
GAAGATGACGGACGCGGCATCCCGGTAGGCATCCACGAAGAAGAGGGTGTCTCCGCCGCCCAGGTCGTCATGACCAAACT
ACACGCCGGCGGAAAATTCGATTCCAACAGTTACAAAGTCTCCGGCGGCCTGCATGGCGTAGGTGTGAGCTGCGTCAACG
CGCTCTCCGAAAAGCTGGAGCTCGAGATCTGGCGCGAAGGCTACACCTGGACGCAGGAGTATTCCTGCGGCAAGCCGAAA
GCAGACCTGGTGCGCGCCGGCAAGGCCGGCAAGCGTACCGGCACCCGCGTCACCTTCAAGCCCGACTCCACCATCATGGA
GGTCGCGGTCTTCAACTACGACACGCTCGCGCAGCGTTTGCGCGAACTGGCCTTTCTCAACAAAGGCCTCAAGATCACCC
TCACCGATGAGCGCGTGGATCCCGAAAAAGTCACGCCCTTCGAATACCAGGGCGGCATCAGCGAATTCATCAAGCACCTC
AATCGCGGCAAGAGCGTCCTGCACGAGAAACCCATCCACTTCGAAGCCGAGCGCGAGATGCCCGAAAAGGGCACGATCAC
GATGGAAGTGGCGCTGCAATACAACGACGCCTATTCGGAAAACGTCTTCAGTTTCGCCAACAACATCAACACGGTGGATG
GCGGTACGCACCTGAGCGGCTTCCGCAGCGCGCTCACCCGCACCATCAACGCCTTCGGCCAGAAAAACGGCCTGTTCAAG
GACGTGAAAGAGAATCTCTCCGGCGACGACGTACGCGAAGGTCTCACCGCGGTGGTCAGCGTCAAAGTGCCGCAGCCGCA
GTTCGAAGGCCAGACCAAGGGCAAGCTGAACAGCGACATCGCCGGTTACATGACGCAGTTCGTCAACGATAAGCTGAGCG
AGTACTTCGACAAGAACTCGGCCGTGGGGCGCAAGATCGTCGGCAAGGCGATCGAGGCCTCCCGCGCCCGTGAGGCCGCC
CGCAAAGCGCGAGATCTGACGCGACGCAAAGGCGCGCTCGACTCTGGCGGCCTGCCTGGCAAGCTCGCTGATTGCCAGGA
GAAAGATCCCAAGCTCTGCGAGCTCTTCCTGGTCGAGGGCGAAAGCGCCGGCGGCACCGCCAAACAGGGCCGCGAACGCC
GCTTCCAGGCTATCCTCCCGCTCAAGGGCAAGATCCTCAACGTGGAGAAGGCCCGCTACGATAAGATGCTGGGCCACGAG
GAAATCCGCTGCATGATCACGGCCCTCGGCACCGGCATCGGCAAGGACGATTTCGACGTCGCCAAACTCCGCTACGACAA
AATCATTATCATGACCGACGCGGACGTGGACGGCTCCCACATCCGTACCCTGCTGCTCACCTTCTTCTTCCGCCACATGA
ACGAGCTCATCACGCGCGGCAAGGTCTACATCGCCCAGCCCCCGTTGTATCGCATCAAGAAGGGCAAGAGCGAAAAGTAC
ATCAAGGACGAGAAGGAGTTCACCAAGGAAATCATGCGCCGCGCGACGGAGAACCTGACCGTCGAGATCCATTCCAACGG
CGATGGTAAACCCAAGTCCTCCCTGGAAGGCCAGGAGCTGCGTACTTTCCTGCTCAACCTCGACGAATTCCAACAGATCT
TCCATAAGGTAGAACGTCGCCTGCGTGATTCCCGCGCCGTCGATGTACTGACCAATGTCGATTTGCATGTCGATAACAAG
GCCGAGTTCCAGGTCGAGGCGAATCTCAAACCGGTATTCGAGGCCATGAAGGCCAAGGGTCTCAATCCGCAGATGAAGAA
GGATGAAGAGCACTCCGCCTGGGCCGTGGTCTTTCACGATTCCACCGGCGCGGAGCGCAGCGTTGGCCTCCAACTGGCGG
CGCAGCCCGAGTACCGCCGCTTCCGCGCCCTGGCCCGGACCATCGCCAAGTTCAACGAGCCGCCCTTCGTCGTGGTGAAG
AACGAGCACCGCGATGCCCAGCCCGATTGGGTCGAGCTCCTCGAGTACGTCAAGAACGAGGGCAAGAAAGACGCCAGCGT
CCAGCGCTACAAGGGTCTCGGCGAAATGAACGCCGAACAGCTCGCGGACACTACCATGAATCCCGAAAAGCGGACCCTCC
TCGAAGTTCGCCTCGAAGACGCCGTCCAGTGCGAAGAGATCTTCTCCACCCTAATGGGTGAAGACGTCGAAAGCCGCCGC
AAATTCATCGAAGAAAACGCCCTCGACGTAAAGAACCTGGACGTCTAG

Upstream 100 bases:

>100_bases
GCGGGCGAACTCCGTCCCGCCGGCGATGCCATCGCGGACCAGTACCGCTATGTAGTCATGCCGATGCGTATTTGAAGTTT
AGTCAAACCAGTAAGGAACT

Downstream 100 bases:

>100_bases
TCCTTACATCCCTTGCAGCGCCTGCTTGACATCGGCCGTCCCCCAAGCCGCGTCGAGCTCGTGCTGTAACTCCGCGATAC
CCTCACTTTTCTTTTGGGCC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 815; Mature: 814

Protein sequence:

>815_residues
MPNEVNPVGGNGNVYDSSSIKVLEGLEAVRLRPAMYIGSTGEMGLHHLVYEVVDNSVDEALAGYATHIEVTIHDDNSVTV
EDDGRGIPVGIHEEEGVSAAQVVMTKLHAGGKFDSNSYKVSGGLHGVGVSCVNALSEKLELEIWREGYTWTQEYSCGKPK
ADLVRAGKAGKRTGTRVTFKPDSTIMEVAVFNYDTLAQRLRELAFLNKGLKITLTDERVDPEKVTPFEYQGGISEFIKHL
NRGKSVLHEKPIHFEAEREMPEKGTITMEVALQYNDAYSENVFSFANNINTVDGGTHLSGFRSALTRTINAFGQKNGLFK
DVKENLSGDDVREGLTAVVSVKVPQPQFEGQTKGKLNSDIAGYMTQFVNDKLSEYFDKNSAVGRKIVGKAIEASRAREAA
RKARDLTRRKGALDSGGLPGKLADCQEKDPKLCELFLVEGESAGGTAKQGRERRFQAILPLKGKILNVEKARYDKMLGHE
EIRCMITALGTGIGKDDFDVAKLRYDKIIIMTDADVDGSHIRTLLLTFFFRHMNELITRGKVYIAQPPLYRIKKGKSEKY
IKDEKEFTKEIMRRATENLTVEIHSNGDGKPKSSLEGQELRTFLLNLDEFQQIFHKVERRLRDSRAVDVLTNVDLHVDNK
AEFQVEANLKPVFEAMKAKGLNPQMKKDEEHSAWAVVFHDSTGAERSVGLQLAAQPEYRRFRALARTIAKFNEPPFVVVK
NEHRDAQPDWVELLEYVKNEGKKDASVQRYKGLGEMNAEQLADTTMNPEKRTLLEVRLEDAVQCEEIFSTLMGEDVESRR
KFIEENALDVKNLDV

Sequences:

>Translated_815_residues
MPNEVNPVGGNGNVYDSSSIKVLEGLEAVRLRPAMYIGSTGEMGLHHLVYEVVDNSVDEALAGYATHIEVTIHDDNSVTV
EDDGRGIPVGIHEEEGVSAAQVVMTKLHAGGKFDSNSYKVSGGLHGVGVSCVNALSEKLELEIWREGYTWTQEYSCGKPK
ADLVRAGKAGKRTGTRVTFKPDSTIMEVAVFNYDTLAQRLRELAFLNKGLKITLTDERVDPEKVTPFEYQGGISEFIKHL
NRGKSVLHEKPIHFEAEREMPEKGTITMEVALQYNDAYSENVFSFANNINTVDGGTHLSGFRSALTRTINAFGQKNGLFK
DVKENLSGDDVREGLTAVVSVKVPQPQFEGQTKGKLNSDIAGYMTQFVNDKLSEYFDKNSAVGRKIVGKAIEASRAREAA
RKARDLTRRKGALDSGGLPGKLADCQEKDPKLCELFLVEGESAGGTAKQGRERRFQAILPLKGKILNVEKARYDKMLGHE
EIRCMITALGTGIGKDDFDVAKLRYDKIIIMTDADVDGSHIRTLLLTFFFRHMNELITRGKVYIAQPPLYRIKKGKSEKY
IKDEKEFTKEIMRRATENLTVEIHSNGDGKPKSSLEGQELRTFLLNLDEFQQIFHKVERRLRDSRAVDVLTNVDLHVDNK
AEFQVEANLKPVFEAMKAKGLNPQMKKDEEHSAWAVVFHDSTGAERSVGLQLAAQPEYRRFRALARTIAKFNEPPFVVVK
NEHRDAQPDWVELLEYVKNEGKKDASVQRYKGLGEMNAEQLADTTMNPEKRTLLEVRLEDAVQCEEIFSTLMGEDVESRR
KFIEENALDVKNLDV
>Mature_814_residues
PNEVNPVGGNGNVYDSSSIKVLEGLEAVRLRPAMYIGSTGEMGLHHLVYEVVDNSVDEALAGYATHIEVTIHDDNSVTVE
DDGRGIPVGIHEEEGVSAAQVVMTKLHAGGKFDSNSYKVSGGLHGVGVSCVNALSEKLELEIWREGYTWTQEYSCGKPKA
DLVRAGKAGKRTGTRVTFKPDSTIMEVAVFNYDTLAQRLRELAFLNKGLKITLTDERVDPEKVTPFEYQGGISEFIKHLN
RGKSVLHEKPIHFEAEREMPEKGTITMEVALQYNDAYSENVFSFANNINTVDGGTHLSGFRSALTRTINAFGQKNGLFKD
VKENLSGDDVREGLTAVVSVKVPQPQFEGQTKGKLNSDIAGYMTQFVNDKLSEYFDKNSAVGRKIVGKAIEASRAREAAR
KARDLTRRKGALDSGGLPGKLADCQEKDPKLCELFLVEGESAGGTAKQGRERRFQAILPLKGKILNVEKARYDKMLGHEE
IRCMITALGTGIGKDDFDVAKLRYDKIIIMTDADVDGSHIRTLLLTFFFRHMNELITRGKVYIAQPPLYRIKKGKSEKYI
KDEKEFTKEIMRRATENLTVEIHSNGDGKPKSSLEGQELRTFLLNLDEFQQIFHKVERRLRDSRAVDVLTNVDLHVDNKA
EFQVEANLKPVFEAMKAKGLNPQMKKDEEHSAWAVVFHDSTGAERSVGLQLAAQPEYRRFRALARTIAKFNEPPFVVVKN
EHRDAQPDWVELLEYVKNEGKKDASVQRYKGLGEMNAEQLADTTMNPEKRTLLEVRLEDAVQCEEIFSTLMGEDVESRRK
FIEENALDVKNLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=592, Percent_Identity=26.0135135135135, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI19913408, Length=574, Percent_Identity=25.609756097561, Blast_Score=131, Evalue=3e-30,
Organism=Escherichia coli, GI48994957, Length=830, Percent_Identity=51.0843373493976, Blast_Score=754, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=576, Percent_Identity=41.6666666666667, Blast_Score=401, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17535065, Length=585, Percent_Identity=25.982905982906, Blast_Score=138, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI212645845, Length=509, Percent_Identity=24.75442043222, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI212645657, Length=155, Percent_Identity=33.5483870967742, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324241, Length=660, Percent_Identity=25.1515151515152, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17136538, Length=564, Percent_Identity=26.9503546099291, Blast_Score=139, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 91215; Mature: 91083

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNEVNPVGGNGNVYDSSSIKVLEGLEAVRLRPAMYIGSTGEMGLHHLVYEVVDNSVDEA
CCCCCCCCCCCCCEECCCCHHHHHCHHHHHCCCEEEECCCCCHHHHHHHHHHHHCHHHHH
LAGYATHIEVTIHDDNSVTVEDDGRGIPVGIHEEEGVSAAQVVMTKLHAGGKFDSNSYKV
HHCCEEEEEEEEECCCEEEECCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEE
SGGLHGVGVSCVNALSEKLELEIWREGYTWTQEYSCGKPKADLVRAGKAGKRTGTRVTFK
CCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEC
PDSTIMEVAVFNYDTLAQRLRELAFLNKGLKITLTDERVDPEKVTPFEYQGGISEFIKHL
CCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHH
NRGKSVLHEKPIHFEAEREMPEKGTITMEVALQYNDAYSENVFSFANNINTVDGGTHLSG
HHCHHHHHCCCCCEEHHCCCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCHHHH
FRSALTRTINAFGQKNGLFKDVKENLSGDDVREGLTAVVSVKVPQPQFEGQTKGKLNSDI
HHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHCCEEEEEEECCCCCCCCCCCCCCCCHH
AGYMTQFVNDKLSEYFDKNSAVGRKIVGKAIEASRAREAARKARDLTRRKGALDSGGLPG
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KLADCQEKDPKLCELFLVEGESAGGTAKQGRERRFQAILPLKGKILNVEKARYDKMLGHE
CCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHEECCCCCEEECHHHHHHHHHHCHH
EIRCMITALGTGIGKDDFDVAKLRYDKIIIMTDADVDGSHIRTLLLTFFFRHMNELITRG
HHHHHHHHHHCCCCCCCCHHHHEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
KVYIAQPPLYRIKKGKSEKYIKDEKEFTKEIMRRATENLTVEIHSNGDGKPKSSLEGQEL
EEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHH
RTFLLNLDEFQQIFHKVERRLRDSRAVDVLTNVDLHVDNKAEFQVEANLKPVFEAMKAKG
HHHHHCHHHHHHHHHHHHHHHHHHHHEEEEECCEEEECCCCEEEEECCCHHHHHHHHHCC
LNPQMKKDEEHSAWAVVFHDSTGAERSVGLQLAAQPEYRRFRALARTIAKFNEPPFVVVK
CCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEE
NEHRDAQPDWVELLEYVKNEGKKDASVQRYKGLGEMNAEQLADTTMNPEKRTLLEVRLED
CCCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHH
AVQCEEIFSTLMGEDVESRRKFIEENALDVKNLDV
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PNEVNPVGGNGNVYDSSSIKVLEGLEAVRLRPAMYIGSTGEMGLHHLVYEVVDNSVDEA
CCCCCCCCCCCCEECCCCHHHHHCHHHHHCCCEEEECCCCCHHHHHHHHHHHHCHHHHH
LAGYATHIEVTIHDDNSVTVEDDGRGIPVGIHEEEGVSAAQVVMTKLHAGGKFDSNSYKV
HHCCEEEEEEEEECCCEEEECCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEE
SGGLHGVGVSCVNALSEKLELEIWREGYTWTQEYSCGKPKADLVRAGKAGKRTGTRVTFK
CCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEEEC
PDSTIMEVAVFNYDTLAQRLRELAFLNKGLKITLTDERVDPEKVTPFEYQGGISEFIKHL
CCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHH
NRGKSVLHEKPIHFEAEREMPEKGTITMEVALQYNDAYSENVFSFANNINTVDGGTHLSG
HHCHHHHHCCCCCEEHHCCCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCCHHHH
FRSALTRTINAFGQKNGLFKDVKENLSGDDVREGLTAVVSVKVPQPQFEGQTKGKLNSDI
HHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHCCEEEEEEECCCCCCCCCCCCCCCCHH
AGYMTQFVNDKLSEYFDKNSAVGRKIVGKAIEASRAREAARKARDLTRRKGALDSGGLPG
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KLADCQEKDPKLCELFLVEGESAGGTAKQGRERRFQAILPLKGKILNVEKARYDKMLGHE
CCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHEECCCCCEEECHHHHHHHHHHCHH
EIRCMITALGTGIGKDDFDVAKLRYDKIIIMTDADVDGSHIRTLLLTFFFRHMNELITRG
HHHHHHHHHHCCCCCCCCHHHHEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
KVYIAQPPLYRIKKGKSEKYIKDEKEFTKEIMRRATENLTVEIHSNGDGKPKSSLEGQEL
EEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHH
RTFLLNLDEFQQIFHKVERRLRDSRAVDVLTNVDLHVDNKAEFQVEANLKPVFEAMKAKG
HHHHHCHHHHHHHHHHHHHHHHHHHHEEEEECCEEEECCCCEEEEECCCHHHHHHHHHCC
LNPQMKKDEEHSAWAVVFHDSTGAERSVGLQLAAQPEYRRFRALARTIAKFNEPPFVVVK
CCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEE
NEHRDAQPDWVELLEYVKNEGKKDASVQRYKGLGEMNAEQLADTTMNPEKRTLLEVRLED
CCCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHH
AVQCEEIFSTLMGEDVESRRKFIEENALDVKNLDV
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]