BacMap

Definition	Lactobacillus casei ATCC 334, complete genome.
Accession	NC_008526
Length	2,895,264

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The map label for this gene is ygcW [H]

Identifier: 116496099

GI number: 116496099

Start: 2657665

End: 2658489

Strand: Direct

Name: ygcW [H]

Synonym: LSEI_2672

Alternate gene names: 116496099

Gene position: 2657665-2658489 (Clockwise)

Preceding gene: 116496098

Following gene: 116496100

Centisome position: 91.79

GC content: 45.94

Gene sequence:

>825_bases
ATGGCTGAAATTCATCAAACTGAAGAAGAAATCCGGAAACACGAGCAAGAACTGGATCATTTCAAAATGTCACAATTTAA
TCTCGCGGGCAGGGTTGCGATTGTCACTGGCGGTAACACTGGTTTAGGCCAAGGTTACGCTGTTGCTATGGCGGAAGCTG
GCGCTGATATCTTTATCCCAACCTTTGGCAAGGCCGAGTGGGACAACACGCGGCGTTTAATTGAAAAGCGTGGGCGTAAA
GTCGCATTCATGGACGTCAATCTCACCGCGCCAGGAAGTGCAGAAGCAGTTGTTGCAGAAGCCATCAAGCAGATGGGCCA
TATTGATATCTTAGTGAACAATGCGGGGATGATTCGCCGCAATCCACTACTTGAATCCAAAGATAAAGATTGGGATCAAG
TGATTGCGATTAATTTAAGCAGCGTCTATCACTTGTCCTTAGCAGTTTCAAAGGAATATGCCAAACAACGATCTGGCAAG
ATAATCAACATCGGCTCCATGCTCTCTTTCCAAGGTGGCAAATTTATTCCTTCTTATGCCGCAAGCAAACACGGCGTCGC
AGGTTTAACGAAGGCTTTTGCTTCAGAAATGGGTGCGTATAATGTCCAAATCAATGCGATTGCTCCAGGCTACATTAAAA
CGGAAAACACCGCACCGATTCGCGCGGACAAAGCGCGTAACGATGAAATTTTGGAACGAATTCCAGCTCGTCATTGGGCA
GAACCTTACGAACTAATGGGCATTGCCGTTTTCTTAGCGAGTGATGCTTCAAACTATATCAATGGCGCTATTATTCCAGT
TGACGGTGGTTATTTGGTTAGCTGA

Upstream 100 bases:

>100_bases
GCAATGTGTGGCGAAAACCAAACCTATGATGACATGGATCAAGTACCCATGAATGAATTACGTTAGCAACAGATAACCCT
ATCAAAAAGGAGCAATACAC

Downstream 100 bases:

>100_bases
AGTCGTTTTGAACCATTTTTTCGGTTAAGTGTATTGACTTCGATTATGACGTATCGTCCTGCTTGAAGCTCTTTTGCGAA
AAAACCATCCTCTGGAAAGA

Product: 2-deoxy-D-gluconate 3-dehydrogenase

Products: 3-dehydro-2-deoxy-D-gluconate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MAEIHQTEEEIRKHEQELDHFKMSQFNLAGRVAIVTGGNTGLGQGYAVAMAEAGADIFIPTFGKAEWDNTRRLIEKRGRK
VAFMDVNLTAPGSAEAVVAEAIKQMGHIDILVNNAGMIRRNPLLESKDKDWDQVIAINLSSVYHLSLAVSKEYAKQRSGK
IINIGSMLSFQGGKFIPSYAASKHGVAGLTKAFASEMGAYNVQINAIAPGYIKTENTAPIRADKARNDEILERIPARHWA
EPYELMGIAVFLASDASNYINGAIIPVDGGYLVS

Sequences:

>Translated_274_residues
MAEIHQTEEEIRKHEQELDHFKMSQFNLAGRVAIVTGGNTGLGQGYAVAMAEAGADIFIPTFGKAEWDNTRRLIEKRGRK
VAFMDVNLTAPGSAEAVVAEAIKQMGHIDILVNNAGMIRRNPLLESKDKDWDQVIAINLSSVYHLSLAVSKEYAKQRSGK
IINIGSMLSFQGGKFIPSYAASKHGVAGLTKAFASEMGAYNVQINAIAPGYIKTENTAPIRADKARNDEILERIPARHWA
EPYELMGIAVFLASDASNYINGAIIPVDGGYLVS
>Mature_273_residues
AEIHQTEEEIRKHEQELDHFKMSQFNLAGRVAIVTGGNTGLGQGYAVAMAEAGADIFIPTFGKAEWDNTRRLIEKRGRKV
AFMDVNLTAPGSAEAVVAEAIKQMGHIDILVNNAGMIRRNPLLESKDKDWDQVIAINLSSVYHLSLAVSKEYAKQRSGKI
INIGSMLSFQGGKFIPSYAASKHGVAGLTKAFASEMGAYNVQINAIAPGYIKTENTAPIRADKARNDEILERIPARHWAE
PYELMGIAVFLASDASNYINGAIIPVDGGYLVS

Specific function: Unknown

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=247, Percent_Identity=30.3643724696356, Blast_Score=120, Evalue=9e-28,
Organism=Homo sapiens, GI15277342, Length=265, Percent_Identity=31.3207547169811, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI7705925, Length=248, Percent_Identity=29.8387096774194, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI5031737, Length=246, Percent_Identity=28.8617886178862, Blast_Score=104, Evalue=8e-23,
Organism=Homo sapiens, GI19923817, Length=251, Percent_Identity=27.8884462151394, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI4503301, Length=198, Percent_Identity=31.8181818181818, Blast_Score=98, Evalue=6e-21,
Organism=Homo sapiens, GI126723750, Length=247, Percent_Identity=27.5303643724696, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI4504505, Length=207, Percent_Identity=31.8840579710145, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI66933014, Length=250, Percent_Identity=29.2, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI40254992, Length=244, Percent_Identity=27.4590163934426, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI33667109, Length=198, Percent_Identity=29.7979797979798, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI10190704, Length=245, Percent_Identity=26.530612244898, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI20149619, Length=205, Percent_Identity=31.7073170731707, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI59889578, Length=205, Percent_Identity=31.219512195122, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI7706318, Length=195, Percent_Identity=25.1282051282051, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI31542939, Length=252, Percent_Identity=25.7936507936508, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI126723191, Length=184, Percent_Identity=26.6304347826087, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI4503817, Length=205, Percent_Identity=27.8048780487805, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI224922801, Length=170, Percent_Identity=27.6470588235294, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI4758504, Length=197, Percent_Identity=25.8883248730964, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI209862901, Length=253, Percent_Identity=26.4822134387352, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI19923983, Length=253, Percent_Identity=26.4822134387352, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI87082160, Length=260, Percent_Identity=49.2307692307692, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI1789208, Length=251, Percent_Identity=49.800796812749, Blast_Score=240, Evalue=8e-65,
Organism=Escherichia coli, GI1790717, Length=249, Percent_Identity=38.9558232931727, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1787335, Length=250, Percent_Identity=36.8, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI87082100, Length=254, Percent_Identity=32.6771653543307, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788459, Length=246, Percent_Identity=29.6747967479675, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787905, Length=250, Percent_Identity=29.6, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1789378, Length=247, Percent_Identity=27.9352226720648, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI2367365, Length=249, Percent_Identity=26.1044176706827, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786812, Length=254, Percent_Identity=25.9842519685039, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI2367175, Length=248, Percent_Identity=25.8064516129032, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787526, Length=202, Percent_Identity=24.7524752475248, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1789057, Length=253, Percent_Identity=23.3201581027668, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25147288, Length=249, Percent_Identity=34.136546184739, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17555706, Length=253, Percent_Identity=32.0158102766798, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17561402, Length=251, Percent_Identity=29.8804780876494, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560676, Length=247, Percent_Identity=25.1012145748988, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115534694, Length=250, Percent_Identity=26, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71994600, Length=250, Percent_Identity=27.6, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17562990, Length=267, Percent_Identity=28.8389513108614, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17536651, Length=252, Percent_Identity=29.3650793650794, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI193204405, Length=267, Percent_Identity=28.4644194756554, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17562908, Length=264, Percent_Identity=29.5454545454545, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17560220, Length=269, Percent_Identity=29.368029739777, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17560150, Length=262, Percent_Identity=26.7175572519084, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI71994604, Length=246, Percent_Identity=27.6422764227642, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17536025, Length=252, Percent_Identity=28.968253968254, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17562906, Length=266, Percent_Identity=29.6992481203008, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17562910, Length=268, Percent_Identity=26.865671641791, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17568967, Length=201, Percent_Identity=28.8557213930348, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17564282, Length=188, Percent_Identity=27.6595744680851, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17551412, Length=257, Percent_Identity=26.4591439688716, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17538486, Length=269, Percent_Identity=27.5092936802974, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17562904, Length=261, Percent_Identity=26.4367816091954, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17561272, Length=175, Percent_Identity=32, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17531453, Length=259, Percent_Identity=27.7992277992278, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI72000259, Length=262, Percent_Identity=27.8625954198473, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17559104, Length=269, Percent_Identity=27.1375464684015, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538480, Length=272, Percent_Identity=26.1029411764706, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560332, Length=266, Percent_Identity=27.8195488721804, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17563726, Length=263, Percent_Identity=27.7566539923954, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71997099, Length=211, Percent_Identity=28.9099526066351, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193203117, Length=191, Percent_Identity=25.6544502617801, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI193203115, Length=190, Percent_Identity=25.7894736842105, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17544670, Length=270, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17538182, Length=253, Percent_Identity=26.4822134387352, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17565030, Length=256, Percent_Identity=25, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17558006, Length=222, Percent_Identity=25.6756756756757, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17555612, Length=214, Percent_Identity=29.4392523364486, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17508895, Length=191, Percent_Identity=24.0837696335079, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI32563809, Length=222, Percent_Identity=24.7747747747748, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17507613, Length=222, Percent_Identity=24.7747747747748, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=189, Percent_Identity=31.2169312169312, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6324126, Length=247, Percent_Identity=24.2914979757085, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24639444, Length=252, Percent_Identity=32.9365079365079, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI21355319, Length=247, Percent_Identity=28.7449392712551, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24644339, Length=253, Percent_Identity=27.6679841897233, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI23397609, Length=249, Percent_Identity=28.1124497991968, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28571526, Length=253, Percent_Identity=28.0632411067194, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24643142, Length=251, Percent_Identity=28.6852589641434, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI21357041, Length=245, Percent_Identity=28.9795918367347, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI18859837, Length=189, Percent_Identity=34.3915343915344, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24644337, Length=189, Percent_Identity=27.5132275132275, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17737361, Length=251, Percent_Identity=25.8964143426295, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28574402, Length=205, Percent_Identity=27.3170731707317, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24584744, Length=205, Percent_Identity=27.3170731707317, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21358495, Length=206, Percent_Identity=25.7281553398058, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21355631, Length=205, Percent_Identity=23.4146341463415, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI161076933, Length=207, Percent_Identity=26.5700483091787, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24584748, Length=207, Percent_Identity=26.5700483091787, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI116007236, Length=195, Percent_Identity=26.1538461538462, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24649181, Length=195, Percent_Identity=23.0769230769231, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24665243, Length=180, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24640492, Length=214, Percent_Identity=24.7663551401869, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI221331218, Length=174, Percent_Identity=27.5862068965517, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.125

Molecular weight: Translated: 29848; Mature: 29717

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIHQTEEEIRKHEQELDHFKMSQFNLAGRVAIVTGGNTGLGQGYAVAMAEAGADIFIP
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEECCCCEEEEE
TFGKAEWDNTRRLIEKRGRKVAFMDVNLTAPGSAEAVVAEAIKQMGHIDILVNNAGMIRR
CCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCEEC
NPLLESKDKDWDQVIAINLSSVYHLSLAVSKEYAKQRSGKIINIGSMLSFQGGKFIPSYA
CCCCCCCCCCCCEEEEEEECCEEEEEEHHHHHHHHHHCCCEEEECCEEECCCCCCCCCHH
ASKHGVAGLTKAFASEMGAYNVQINAIAPGYIKTENTAPIRADKARNDEILERIPARHWA
CCCCCHHHHHHHHHHHCCCEEEEEEEECCCEEECCCCCCCCCCCCCCHHHHHHCCHHHCC
EPYELMGIAVFLASDASNYINGAIIPVDGGYLVS
CHHHHHEEEEEEECCCCHHHCCEEEEECCCEECC
>Mature Secondary Structure 
AEIHQTEEEIRKHEQELDHFKMSQFNLAGRVAIVTGGNTGLGQGYAVAMAEAGADIFIP
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEECCCCEEEEE
TFGKAEWDNTRRLIEKRGRKVAFMDVNLTAPGSAEAVVAEAIKQMGHIDILVNNAGMIRR
CCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCEEC
NPLLESKDKDWDQVIAINLSSVYHLSLAVSKEYAKQRSGKIINIGSMLSFQGGKFIPSYA
CCCCCCCCCCCCEEEEEEECCEEEEEEHHHHHHHHHHCCCEEEECCEEECCCCCCCCCHH
ASKHGVAGLTKAFASEMGAYNVQINAIAPGYIKTENTAPIRADKARNDEILERIPARHWA
CCCCCHHHHHHHHHHHCCCEEEEEEEECCCEEECCCCCCCCCCCCCCHHHHHHCCHHHCC
EPYELMGIAVFLASDASNYINGAIIPVDGGYLVS
CHHHHHEEEEEEECCCCHHHCCEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-deoxy-D-gluconate; NAD+

Specific reaction: 2-deoxy-D-gluconate + NAD+ = 3-dehydro-2-deoxy-D-gluconate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9205837 [H]