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The map label for this gene is yiaJ [H]

Identifier: 116496097

GI number: 116496097

Start: 2655756

End: 2656523

Strand: Direct

Name: yiaJ [H]

Synonym: LSEI_2670

Alternate gene names: 116496097

Gene position: 2655756-2656523 (Clockwise)

Preceding gene: 116496083

Following gene: 116496098

Centisome position: 91.73

GC content: 44.92

Gene sequence:

>768_bases
ATGGGCACCACAACAAATGAGTCAAAACCTTATGGGACCGTGCTGATCAAGGCTAAAGATATTTTAGATTTCCTACTTGC
CACTACTGATCCACCAACCTTATCTGACATTAGTCGCGGACTGCATGCTTCGAAACCGACCATTTTAAAGATCTTAGGAA
CACTTGAATCGCTTGGCTTTGTTTGGCGGGATACCACGACAAAACAATATTTTCTCGGCACGCAGTTTGTGCCATATGCG
CAGAAAGCAATTGCCACTTTTAATATTGTCAGAGTCGCACGTCCTTATCTCGAAGATTTGCGGGATAAGACGCAAGAAAC
CATTAACTTAGGGATTGTCCGCGATAATAAGATGATCCTTGTTGAAAAATTGGAAAGCCCCACCAGCATCAAGTTGCAAT
CAACTATTGGCGGAAGCATGAACATGTACTCGTCTGCAATGGGAAAAGCCGTTCTAGCCACGTATGATGCCAAGGAACTA
CAGGCTTACTTCAAGTCTCACAAACTCACGCCCATGACACCGCATACGATCACTACACCCACAAAACTTCAGCAAGATCT
AAAAACGATTCAAGAGCTCGGTGTCGCGATCGATAATGAGGAAAACGAGGAAGAAGTTTATTGTCTCGGTGCTGCACTAC
AAAAAAACGGTGAACTTTATGGTGCCTTCAGCATTAGCACACCGAAGTATCGGCTTCCCAAGGAACGTCGAGCTGCCTTT
GTCCGCTTGCTGTTGGACACACGCCACGCAATTGAAAATACCCTTTAA

Upstream 100 bases:

>100_bases
ATTATCCATAATGTCGGATTAGTATTGATTTTTTTGCTGATTGTGACTAAAATGATCAGTAGTTTCCAAATAATAAACTC
TTACAACTGGAGGGATACGC

Downstream 100 bases:

>100_bases
CTTTCAAGATACTTTTTGAAATTGTTTACCTTACGCAATCAAAACAAACAGGCCCCAGAAAGACATTGCCTCGTCTCTCT
GGGGCCTGTTTAGTTTGTTC

Product: transcriptional regulator

Products: NA

Alternate protein names: YiaKLMNOPQRS operon repressor; YiaK-S operon repressor [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MGTTTNESKPYGTVLIKAKDILDFLLATTDPPTLSDISRGLHASKPTILKILGTLESLGFVWRDTTTKQYFLGTQFVPYA
QKAIATFNIVRVARPYLEDLRDKTQETINLGIVRDNKMILVEKLESPTSIKLQSTIGGSMNMYSSAMGKAVLATYDAKEL
QAYFKSHKLTPMTPHTITTPTKLQQDLKTIQELGVAIDNEENEEEVYCLGAALQKNGELYGAFSISTPKYRLPKERRAAF
VRLLLDTRHAIENTL

Sequences:

>Translated_255_residues
MGTTTNESKPYGTVLIKAKDILDFLLATTDPPTLSDISRGLHASKPTILKILGTLESLGFVWRDTTTKQYFLGTQFVPYA
QKAIATFNIVRVARPYLEDLRDKTQETINLGIVRDNKMILVEKLESPTSIKLQSTIGGSMNMYSSAMGKAVLATYDAKEL
QAYFKSHKLTPMTPHTITTPTKLQQDLKTIQELGVAIDNEENEEEVYCLGAALQKNGELYGAFSISTPKYRLPKERRAAF
VRLLLDTRHAIENTL
>Mature_254_residues
GTTTNESKPYGTVLIKAKDILDFLLATTDPPTLSDISRGLHASKPTILKILGTLESLGFVWRDTTTKQYFLGTQFVPYAQ
KAIATFNIVRVARPYLEDLRDKTQETINLGIVRDNKMILVEKLESPTSIKLQSTIGGSMNMYSSAMGKAVLATYDAKELQ
AYFKSHKLTPMTPHTITTPTKLQQDLKTIQELGVAIDNEENEEEVYCLGAALQKNGELYGAFSISTPKYRLPKERRAAFV
RLLLDTRHAIENTL

Specific function: Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1789999, Length=265, Percent_Identity=32.4528301886792, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788131, Length=212, Percent_Identity=28.3018867924528, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1786715, Length=236, Percent_Identity=27.5423728813559, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786468, Length=225, Percent_Identity=28, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788581, Length=224, Percent_Identity=30.3571428571429, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082359, Length=234, Percent_Identity=22.2222222222222, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790752, Length=196, Percent_Identity=27.5510204081633, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 28477; Mature: 28346

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTTTNESKPYGTVLIKAKDILDFLLATTDPPTLSDISRGLHASKPTILKILGTLESLGF
CCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCE
VWRDTTTKQYFLGTQFVPYAQKAIATFNIVRVARPYLEDLRDKTQETINLGIVRDNKMIL
EECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCEEE
VEKLESPTSIKLQSTIGGSMNMYSSAMGKAVLATYDAKELQAYFKSHKLTPMTPHTITTP
EEECCCCCCEEEEECCCCCHHHHHHHHCCHHHEECCHHHHHHHHHHCCCCCCCCCCCCCH
TKLQQDLKTIQELGVAIDNEENEEEVYCLGAALQKNGELYGAFSISTPKYRLPKERRAAF
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHCCCCEEEEEEECCCCCCCCHHHHHHH
VRLLLDTRHAIENTL
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
GTTTNESKPYGTVLIKAKDILDFLLATTDPPTLSDISRGLHASKPTILKILGTLESLGF
CCCCCCCCCCEEEEEEHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCE
VWRDTTTKQYFLGTQFVPYAQKAIATFNIVRVARPYLEDLRDKTQETINLGIVRDNKMIL
EECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCEEE
VEKLESPTSIKLQSTIGGSMNMYSSAMGKAVLATYDAKELQAYFKSHKLTPMTPHTITTP
EEECCCCCCEEEEECCCCCHHHHHHHHCCHHHEECCHHHHHHHHHHCCCCCCCCCCCCCH
TKLQQDLKTIQELGVAIDNEENEEEVYCLGAALQKNGELYGAFSISTPKYRLPKERRAAF
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHCCCCEEEEEEECCCCCCCCHHHHHHH
VRLLLDTRHAIENTL
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]