Click here to switch to the map view.

The map label for this gene is cysL [H]

Identifier: 116496059

GI number: 116496059

Start: 2617208

End: 2618041

Strand: Direct

Name: cysL [H]

Synonym: LSEI_2631

Alternate gene names: 116496059

Gene position: 2617208-2618041 (Clockwise)

Preceding gene: 116496058

Following gene: 116496063

Centisome position: 90.4

GC content: 48.32

Gene sequence:

>834_bases
TTGTTTAATCTACTCCGTACCTTCATGGCCGTTTACGAAACACGCAGCTTTTCCTTGGCAGCCACGCACTTATTCACCAG
CCAGCCAACTGTGTCACACCATATTCATCAGCTGGAAAATCAACTAAAAACAACCTTATTTGAACGAAACAAACGCAGTG
CGACGATTCCCACAGCAGCTGCTGATATTTTGTATACCTTTAGTGGCAACCTGCTGGCTGAGTGGGAAAAAACGCAGGAA
GCGATTCAAAATAGTCAAAAGGATGCCATTGTTGACCTGCATATTGGCGTATCGCAAAGTGTCGCCGCTGTTCTATTTTC
CCGAATCGCAGCGGCTTTAAAAAAGGCTTTTCCCTACTTGCAATATGATGTCGCCGTGCTCAATTCCTTGCAAGTTGTCA
AACAGCTGGAAGGCCAACATTTAGATTTAGGCTTTATTGAACAACCTTTAACCTTAAAGGGCGCTGTCCGAACAGCCCTT
TGTACGGATCAACTGATCGCTGCAGGTTGGTCTACCGGCACTTGGATTACCCGCGAAGAAGGCTCTGGGATGCGCTATTA
CACCACCGCTTATCTGCAAGCAGCCGGTATTTCGCCGCAACACATGATGACTGTCAATAACAATGCCATGCTGCTGGCAT
TGGTACAACAAGGTGTCGGGCAGGCACTTTTGTCGGATCAAGTCGCACTCGGCAATGCTCCGGCACAAGCAATTGGGCCG
CAATTTCAACGGCATTTTTACTTGCTGCAAAACCCAACCGCCAACTGGCCCCACAACCAGGCCATGCTAGATAAAATTGT
TGAAGCAGCTCGCGCTTCGGAAGTAACCCGCTGA

Upstream 100 bases:

>100_bases
CTTCGCTGACACGGGCGTTTGTTTGGCTTCTAGAATCATGATAAATTATCAATGGAACGATTTAATCAATTCTGTTTATT
AAAAATTTGGAGGTTTATTT

Downstream 100 bases:

>100_bases
TGGCCAAGCTGCTTTGGATGACTATTAATCAGACAGTTCTTGAGTAGCAGTTGCTGTTTGCTTGACTGTTTTAGCTGTAA
GCCGCAAAAAGTTTAGATAA

Product: transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MFNLLRTFMAVYETRSFSLAATHLFTSQPTVSHHIHQLENQLKTTLFERNKRSATIPTAAADILYTFSGNLLAEWEKTQE
AIQNSQKDAIVDLHIGVSQSVAAVLFSRIAAALKKAFPYLQYDVAVLNSLQVVKQLEGQHLDLGFIEQPLTLKGAVRTAL
CTDQLIAAGWSTGTWITREEGSGMRYYTTAYLQAAGISPQHMMTVNNNAMLLALVQQGVGQALLSDQVALGNAPAQAIGP
QFQRHFYLLQNPTANWPHNQAMLDKIVEAARASEVTR

Sequences:

>Translated_277_residues
MFNLLRTFMAVYETRSFSLAATHLFTSQPTVSHHIHQLENQLKTTLFERNKRSATIPTAAADILYTFSGNLLAEWEKTQE
AIQNSQKDAIVDLHIGVSQSVAAVLFSRIAAALKKAFPYLQYDVAVLNSLQVVKQLEGQHLDLGFIEQPLTLKGAVRTAL
CTDQLIAAGWSTGTWITREEGSGMRYYTTAYLQAAGISPQHMMTVNNNAMLLALVQQGVGQALLSDQVALGNAPAQAIGP
QFQRHFYLLQNPTANWPHNQAMLDKIVEAARASEVTR
>Mature_277_residues
MFNLLRTFMAVYETRSFSLAATHLFTSQPTVSHHIHQLENQLKTTLFERNKRSATIPTAAADILYTFSGNLLAEWEKTQE
AIQNSQKDAIVDLHIGVSQSVAAVLFSRIAAALKKAFPYLQYDVAVLNSLQVVKQLEGQHLDLGFIEQPLTLKGAVRTAL
CTDQLIAAGWSTGTWITREEGSGMRYYTTAYLQAAGISPQHMMTVNNNAMLLALVQQGVGQALLSDQVALGNAPAQAIGP
QFQRHFYLLQNPTANWPHNQAMLDKIVEAARASEVTR

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994962, Length=143, Percent_Identity=31.4685314685315, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790208, Length=248, Percent_Identity=25, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790399, Length=256, Percent_Identity=24.21875, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 30583; Mature: 30583

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNLLRTFMAVYETRSFSLAATHLFTSQPTVSHHIHQLENQLKTTLFERNKRSATIPTAA
CHHHHHHHHHHHHCCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
ADILYTFSGNLLAEWEKTQEAIQNSQKDAIVDLHIGVSQSVAAVLFSRIAAALKKAFPYL
HHHHHHCCCCEEHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHH
QYDVAVLNSLQVVKQLEGQHLDLGFIEQPLTLKGAVRTALCTDQLIAAGWSTGTWITREE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
GSGMRYYTTAYLQAAGISPQHMMTVNNNAMLLALVQQGVGQALLSDQVALGNAPAQAIGP
CCCCEEEHEEHHHHCCCCCCEEEEECCCEEEHHHHHHHHHHHHHHCCHHCCCCCHHHHCH
QFQRHFYLLQNPTANWPHNQAMLDKIVEAARASEVTR
HHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFNLLRTFMAVYETRSFSLAATHLFTSQPTVSHHIHQLENQLKTTLFERNKRSATIPTAA
CHHHHHHHHHHHHCCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
ADILYTFSGNLLAEWEKTQEAIQNSQKDAIVDLHIGVSQSVAAVLFSRIAAALKKAFPYL
HHHHHHCCCCEEHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHH
QYDVAVLNSLQVVKQLEGQHLDLGFIEQPLTLKGAVRTALCTDQLIAAGWSTGTWITREE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
GSGMRYYTTAYLQAAGISPQHMMTVNNNAMLLALVQQGVGQALLSDQVALGNAPAQAIGP
CCCCEEEHEEHHHHCCCCCCEEEEECCCEEEHHHHHHHHHHHHHHCCHHCCCCCHHHHCH
QFQRHFYLLQNPTANWPHNQAMLDKIVEAARASEVTR
HHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]