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The map label for this gene is insI1 [C]

Identifier: 116495800

GI number: 116495800

Start: 2349336

End: 2350352

Strand: Direct

Name: insI1 [C]

Synonym: LSEI_2350

Alternate gene names: 116495800

Gene position: 2349336-2350352 (Clockwise)

Preceding gene: 116495799

Following gene: 116495801

Centisome position: 81.14

GC content: 51.92

Gene sequence:

>1017_bases
ATGACCCACTCTCAGACTAACACTCACAAGCATTACCAACAACTCAGTTTTAGCGACCGTGCTACAATTCAGGCCCTTCA
GGCTGCTGGTGACACCGCGACCGTGATTGCACAGAAGCTTCATCGCAGTAAAGCGACAATCTCACGAGAAATCACGCGTG
GATCTGTAACTCAGCTCGACTCGAAGCGTCACTCGCATCAAGTCTATCTTGCGGAAACTGCCCAAGCCATGCACGACCGT
AAACGCGATAGAACCGGTCACTACGCCTTTCTTAAGACCGGCCGTGCGTTCTTCAAGGCTCTCTCCAGGGAGCTTACTCG
TAAGCCGCGCGTACACAGCGTTGATAGCTTCGTACACTTCTATCGCGACCAGGGCAAGGCTTGCCCTTCAACGACAACTG
TGTATCGCTACATCGACGCCGGGCTGCTTGAGCTAGACAACATGACACTTCCCAAGAAGCTCCGACGCCGCATCAAAGGC
TATAAGAACGCCCACAAGCGCAAGAATAAGAAGATATACGGCGACTCAATCGAGTTGCGTCCTGCGGCCGTGAATGACCG
CACAGGCGTGGGACATTGGGAAGGCGACTTGGTCAAGGGTATTCGCTTAGCTGATGAGCCAGCATTAATGACGCTCACAG
AACGGTACAGCCGGACTGAGATCATCGTCAAGATTCCTGACTATCATGCGGGCACCTGCCTTAAAGCCTTGCAGGACACG
ATCGACGACTACGGGGCCAAGGAATTTGAGAGTATCACTTTTGACAATGGTTCCGAGTTTGCCAAGTTATCAGAGATTGT
TGGAACCAAGATTTACTTCGCACATCCGTACTCGCCTTGGGAGCGTGGCACAAACGAGAACGCCAATGGACTGCTTAGGG
AATTCTTCCCGAAAGGGAAGTCTCTCAGAGCAGTTACCCTGGTTGAAATTCAAGCAGTCCAATCCGCACTGAACCATCGT
CCCAGACGTATTCTGAACTATCTTCGCCCATGCGATTACTACCGATGCATGGCGTAA

Upstream 100 bases:

>100_bases
CCAGTTTACAATTCAAGTGCAACACCCTGACGACTAGACCATAAAGGCCATGAAGACCTATTCTTGTTAGTGCTAGCTAA
ACAAGAAAGCAGGAATCTTC

Downstream 100 bases:

>100_bases
CAGCCTAGACCACTATCAAGAATTCGTTATCATCGTTGCACTTGACTTGAAAATTGAGGAGATCTTTATTCATTAACTGG
TCAATTGCACACAATATTTA

Product: IS30 family transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MTHSQTNTHKHYQQLSFSDRATIQALQAAGDTATVIAQKLHRSKATISREITRGSVTQLDSKRHSHQVYLAETAQAMHDR
KRDRTGHYAFLKTGRAFFKALSRELTRKPRVHSVDSFVHFYRDQGKACPSTTTVYRYIDAGLLELDNMTLPKKLRRRIKG
YKNAHKRKNKKIYGDSIELRPAAVNDRTGVGHWEGDLVKGIRLADEPALMTLTERYSRTEIIVKIPDYHAGTCLKALQDT
IDDYGAKEFESITFDNGSEFAKLSEIVGTKIYFAHPYSPWERGTNENANGLLREFFPKGKSLRAVTLVEIQAVQSALNHR
PRRILNYLRPCDYYRCMA

Sequences:

>Translated_338_residues
MTHSQTNTHKHYQQLSFSDRATIQALQAAGDTATVIAQKLHRSKATISREITRGSVTQLDSKRHSHQVYLAETAQAMHDR
KRDRTGHYAFLKTGRAFFKALSRELTRKPRVHSVDSFVHFYRDQGKACPSTTTVYRYIDAGLLELDNMTLPKKLRRRIKG
YKNAHKRKNKKIYGDSIELRPAAVNDRTGVGHWEGDLVKGIRLADEPALMTLTERYSRTEIIVKIPDYHAGTCLKALQDT
IDDYGAKEFESITFDNGSEFAKLSEIVGTKIYFAHPYSPWERGTNENANGLLREFFPKGKSLRAVTLVEIQAVQSALNHR
PRRILNYLRPCDYYRCMA
>Mature_337_residues
THSQTNTHKHYQQLSFSDRATIQALQAAGDTATVIAQKLHRSKATISREITRGSVTQLDSKRHSHQVYLAETAQAMHDRK
RDRTGHYAFLKTGRAFFKALSRELTRKPRVHSVDSFVHFYRDQGKACPSTTTVYRYIDAGLLELDNMTLPKKLRRRIKGY
KNAHKRKNKKIYGDSIELRPAAVNDRTGVGHWEGDLVKGIRLADEPALMTLTERYSRTEIIVKIPDYHAGTCLKALQDTI
DDYGAKEFESITFDNGSEFAKLSEIVGTKIYFAHPYSPWERGTNENANGLLREFFPKGKSLRAVTLVEIQAVQSALNHRP
RRILNYLRPCDYYRCMA

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786450, Length=326, Percent_Identity=30.3680981595092, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790736, Length=326, Percent_Identity=30.0613496932515, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787671, Length=326, Percent_Identity=30.0613496932515, Blast_Score=125, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 38690; Mature: 38559

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS50994 INTEGRASE ; PS01043 TRANSPOSASE_IS30

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSQTNTHKHYQQLSFSDRATIQALQAAGDTATVIAQKLHRSKATISREITRGSVTQLD
CCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SKRHSHQVYLAETAQAMHDRKRDRTGHYAFLKTGRAFFKALSRELTRKPRVHSVDSFVHF
HHHCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
YRDQGKACPSTTTVYRYIDAGLLELDNMTLPKKLRRRIKGYKNAHKRKNKKIYGDSIELR
HHHCCCCCCCHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEC
PAAVNDRTGVGHWEGDLVKGIRLADEPALMTLTERYSRTEIIVKIPDYHAGTCLKALQDT
CCCCCCCCCCCCCCHHHHHCCEECCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
IDDYGAKEFESITFDNGSEFAKLSEIVGTKIYFAHPYSPWERGTNENANGLLREFFPKGK
HHHCCCHHHHCEECCCCHHHHHHHHHHCCEEEEECCCCHHHCCCCCCHHHHHHHHCCCCC
SLRAVTLVEIQAVQSALNHRPRRILNYLRPCDYYRCMA
CEEEEHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
THSQTNTHKHYQQLSFSDRATIQALQAAGDTATVIAQKLHRSKATISREITRGSVTQLD
CCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
SKRHSHQVYLAETAQAMHDRKRDRTGHYAFLKTGRAFFKALSRELTRKPRVHSVDSFVHF
HHHCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
YRDQGKACPSTTTVYRYIDAGLLELDNMTLPKKLRRRIKGYKNAHKRKNKKIYGDSIELR
HHHCCCCCCCHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEC
PAAVNDRTGVGHWEGDLVKGIRLADEPALMTLTERYSRTEIIVKIPDYHAGTCLKALQDT
CCCCCCCCCCCCCCHHHHHCCEECCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
IDDYGAKEFESITFDNGSEFAKLSEIVGTKIYFAHPYSPWERGTNENANGLLREFFPKGK
HHHCCCHHHHCEECCCCHHHHHHHHHHCCEEEEECCCCHHHCCCCCCHHHHHHHHCCCCC
SLRAVTLVEIQAVQSALNHRPRRILNYLRPCDYYRCMA
CEEEEHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8393068 [H]