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The map label for this gene is rnhB

Identifier: 116494884

GI number: 116494884

Start: 1385166

End: 1385924

Strand: Direct

Name: rnhB

Synonym: LSEI_1398

Alternate gene names: 116494884

Gene position: 1385166-1385924 (Clockwise)

Preceding gene: 116494883

Following gene: 116494885

Centisome position: 47.84

GC content: 51.65

Gene sequence:

>759_bases
ATGCCGACGCTGAGTGAGTATAAAGCGTTGCTGGCTGCTGACGAAGTTGCACCGGCTATTTTGGCGGCACTTAAAGAAGA
CAGTCGCATCGGTGCTGGCAAATTACTGGCAGCGTATCAACGGCGACAGGCCCATCAAGCAGCCGAAGAGGTGGCTTTAC
GTTATCGTAGCAGGTATGAGCGTCAGTTATGGGGAACCTATGAGTATGTCGCTGGGTTAGACGAAGTAGGGCGGGGACCA
CTTGCTGGCCCTGTCGTGACGGCGGCGGTGATTTTGCCGCACCATTTTCAGTGGCCGGTGAATGACTCGAAACAGCTGAC
CGCACATGAACGCAATGTGCTTTACCCGCATATTTTAACTGAAGCGATTGCAGTTGGCATTGGCGTGGCTGATAATCGGA
CGATTGACCGTGAAAATATTTACCATGCGACTGAATTGGCGATGGCTCAAGCCGTTGACCATTTGCGAGTCGCTCCGGAA
TGTTTGTTGGTGGACGCAATGCACGTGCCAGTCGACTTACCGCAAAAACGCTTAATTAAGGGCGATGCCAACAGCATCAG
TATCGCTGCGGCCAGTATTGTTGCTAAAGTGATTCGTGATCGGCTTATGATGATGTACGATAAAATTTATCCTGGTTATG
ATTTTAAGGACAACATGGGATACGGGACAAAGGCACATCTTGCCGGATTGGCGGCGCACGGCGTTACCCCAATTCATCGC
CGCAGTTTTGGGCCTGTCCGTGATCGACTGCGCTCTTAG

Upstream 100 bases:

>100_bases
GGTTTCGCTGAGGCTTACGATCGGATGGCTGAGCGCTTGATTTTTGATGCACGACAAGGAAAATTGGGACGCTTTACCCT
CGAGAAGCCTGGTGAGACAG

Downstream 100 bases:

>100_bases
TGCTTCATTGAGTGTGTCGATGTCGTGTTTGCGTAGTTTATTTGCGGGAGGACGACCAACAATGACACTGCGCAATTTTT
TACTAGCATGGCACATTGCC

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MPTLSEYKALLAADEVAPAILAALKEDSRIGAGKLLAAYQRRQAHQAAEEVALRYRSRYERQLWGTYEYVAGLDEVGRGP
LAGPVVTAAVILPHHFQWPVNDSKQLTAHERNVLYPHILTEAIAVGIGVADNRTIDRENIYHATELAMAQAVDHLRVAPE
CLLVDAMHVPVDLPQKRLIKGDANSISIAAASIVAKVIRDRLMMMYDKIYPGYDFKDNMGYGTKAHLAGLAAHGVTPIHR
RSFGPVRDRLRS

Sequences:

>Translated_252_residues
MPTLSEYKALLAADEVAPAILAALKEDSRIGAGKLLAAYQRRQAHQAAEEVALRYRSRYERQLWGTYEYVAGLDEVGRGP
LAGPVVTAAVILPHHFQWPVNDSKQLTAHERNVLYPHILTEAIAVGIGVADNRTIDRENIYHATELAMAQAVDHLRVAPE
CLLVDAMHVPVDLPQKRLIKGDANSISIAAASIVAKVIRDRLMMMYDKIYPGYDFKDNMGYGTKAHLAGLAAHGVTPIHR
RSFGPVRDRLRS
>Mature_251_residues
PTLSEYKALLAADEVAPAILAALKEDSRIGAGKLLAAYQRRQAHQAAEEVALRYRSRYERQLWGTYEYVAGLDEVGRGPL
AGPVVTAAVILPHHFQWPVNDSKQLTAHERNVLYPHILTEAIAVGIGVADNRTIDRENIYHATELAMAQAVDHLRVAPEC
LLVDAMHVPVDLPQKRLIKGDANSISIAAASIVAKVIRDRLMMMYDKIYPGYDFKDNMGYGTKAHLAGLAAHGVTPIHRR
SFGPVRDRLRS

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=185, Percent_Identity=48.6486486486487, Blast_Score=153, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_LACC3 (Q039E6)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806618.1
- ProteinModelPortal:   Q039E6
- SMR:   Q039E6
- STRING:   Q039E6
- GeneID:   4419739
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_1398
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- PhylomeDB:   Q039E6
- ProtClustDB:   PRK00015
- BioCyc:   LCAS321967:LSEI_1398-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 27848; Mature: 27717

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCHHHHHHCC
>Mature Secondary Structure 
PTLSEYKALLAADEVAPAILAALKEDSRIGAGKLLAAYQRRQAHQAAEEVALRYRSRYE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQLWGTYEYVAGLDEVGRGPLAGPVVTAAVILPHHFQWPVNDSKQLTAHERNVLYPHILT
HHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCHHHHHH
EAIAVGIGVADNRTIDRENIYHATELAMAQAVDHLRVAPECLLVDAMHVPVDLPQKRLIK
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHC
GDANSISIAAASIVAKVIRDRLMMMYDKIYPGYDFKDNMGYGTKAHLAGLAAHGVTPIHR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHH
RSFGPVRDRLRS
CCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA