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The map label for this gene is pdhC [H]

Identifier: 116494796

GI number: 116494796

Start: 1289329

End: 1290984

Strand: Direct

Name: pdhC [H]

Synonym: LSEI_1307

Alternate gene names: 116494796

Gene position: 1289329-1290984 (Clockwise)

Preceding gene: 116494795

Following gene: 116494797

Centisome position: 44.53

GC content: 49.46

Gene sequence:

>1656_bases
ATGGCTTTTGAATTCAAACTGCCAGAACTTGGCGAAGGTTTGGCAGAAGGCGAAATCGTTAAGTGGGACGTCAAACCTGG
TGATGACATCAAGGAAGACGATACCTTACTTGAGGTTCAAAGTGATAAAAGTGTTGAAGAGATTCCATCTCCGGTCTCCG
GCAAAATTTTAAAAATTCTTGTTCCAGAAGGGGAAACCGCGTCAGTTGGTGATCTATTGGTTGAAATCGATGATGGCTCC
GGACCGGCAGCTGCACCTGCGACTGCAACAGCAGCCCCTGCGACACCCGCTCCAGCAACGCCTGCTGTGCAACCTGCACC
TGCGCAATCGGTTTATCAGTTTAAGTTACCCGAACTAGGCGAAGGTTTGGCAGAAGGCGAAATTGTTAAATGGACAGTCA
AGCCTGGCGATCAAATCAAAGAGGACGACACGCTGTTAGAAGTTCAAAGCGACAAGAGTGTCGAAGAAATTCCTTCTCCA
GTTGCCGGAACGGTTGTTAAGATTCTGGTACCAGAAGGCGAAACCGCGACAGTCGGTGAAGCGCTGGTTGATATTGATGC
ACCGGGTCATAATGACACATCGGTTGCTACTGAGGCTGGCGCTGCACCGCAGCCGGTTGCTGCAACACCGGCTGCGACGC
CAGCTGCACCAGCAGCGGGTGGCGTGACGGCCATCACCGATCCGAACCGGGAAATTTTAGCAATGCCATCTGTGCGTCAG
TATGCACGCGAACAGGGCATTGACATCTCACAAGTGCCAGCAACCGGCAAACATGGCCGAATTACCAAGGCTGATGTCGA
TGCATTCAAGACGGGCGCACCAGCTGCATCTGCACAGCCAGCAGCCCCTGCTGCTCAGGCACAACCGCAACCAGCCACAG
CAAAGCCAGCTGCACCGGCAGCACCAAAGCCGCAAGCAGTTGCACCATACGTTTCGAGTGGTAATGAAGCTGAACTTGAA
ACCCGTGAAAAGATGACCCCAACTCGGAAAGCGATTGCTAAGGCGATGCTTGCTTCTAAGCAACGTAGCCCGCATGTGAC
GAGTTTTGATGACGTTGAAGTTTCTAAGTTAATGGCTCATCGCAAGAAGTACAAGCAATACGCAGCTGATAAGGGTATCA
AGTTAACCTTCCTGCCGTATATCGTCAAAGCACTTGTGACGGTGTTGCGTGAATATCCAGAATTCAATGCGTCCATTGAT
GATACAACGGATGAAATTGTCTATAAGCATTACTTCAACATTGGGATTGCAACAAATACGGATCACGGCTTGTATGTGCC
TGTGATCAAGAATGCCGATGCAAAGAGTATGTTTGAAATTGCTAAGGAAATTAGTGAAAATGCCCAGAAGGCTTATGACA
GCAAACTAAAGCCGGATGAAATGCGCGGTGGCTCCATGACCATCAGTAACGTTGGGTCAATTGGCGGCGGCTGGTTCACC
CCAGTGATCAATCAACCTGAAGTTGCGATTCTCGGAGTGGGCAAAATTGCTAAGGAGCCTTATGTCAACGAAGACAACGA
AATTGTGGTTGGCAACATGTTGAAATTAAGTTTGAGCTATGACCATCGTTTGATTGACGGCGCGTTAGCACAAACCGCCT
TGAATCTGATGGATAAATTGTTGGCAGATCCTGATCTGCTGTTGATGGAGGGATAA

Upstream 100 bases:

>100_bases
TCCCATTTGGCCAAGCTGAAAACATTTGGCTGCCAAATGCTAAGGATATTGAAGCTAAAGTCCGGGAAACCGTTAATTTC
TAGAAAGAAGAGGTGCCAAC

Downstream 100 bases:

>100_bases
GCATGGTTGTAGGCGATTTTGCAATCGATTTGGATACCGTCGTCATTGGTTCCGGCCCCGGCGGTTATGTTGCTGCGATT
CGCGCAGCTGAAATGGGTCA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MAFEFKLPELGEGLAEGEIVKWDVKPGDDIKEDDTLLEVQSDKSVEEIPSPVSGKILKILVPEGETASVGDLLVEIDDGS
GPAAAPATATAAPATPAPATPAVQPAPAQSVYQFKLPELGEGLAEGEIVKWTVKPGDQIKEDDTLLEVQSDKSVEEIPSP
VAGTVVKILVPEGETATVGEALVDIDAPGHNDTSVATEAGAAPQPVAATPAATPAAPAAGGVTAITDPNREILAMPSVRQ
YAREQGIDISQVPATGKHGRITKADVDAFKTGAPAASAQPAAPAAQAQPQPATAKPAAPAAPKPQAVAPYVSSGNEAELE
TREKMTPTRKAIAKAMLASKQRSPHVTSFDDVEVSKLMAHRKKYKQYAADKGIKLTFLPYIVKALVTVLREYPEFNASID
DTTDEIVYKHYFNIGIATNTDHGLYVPVIKNADAKSMFEIAKEISENAQKAYDSKLKPDEMRGGSMTISNVGSIGGGWFT
PVINQPEVAILGVGKIAKEPYVNEDNEIVVGNMLKLSLSYDHRLIDGALAQTALNLMDKLLADPDLLLMEG

Sequences:

>Translated_551_residues
MAFEFKLPELGEGLAEGEIVKWDVKPGDDIKEDDTLLEVQSDKSVEEIPSPVSGKILKILVPEGETASVGDLLVEIDDGS
GPAAAPATATAAPATPAPATPAVQPAPAQSVYQFKLPELGEGLAEGEIVKWTVKPGDQIKEDDTLLEVQSDKSVEEIPSP
VAGTVVKILVPEGETATVGEALVDIDAPGHNDTSVATEAGAAPQPVAATPAATPAAPAAGGVTAITDPNREILAMPSVRQ
YAREQGIDISQVPATGKHGRITKADVDAFKTGAPAASAQPAAPAAQAQPQPATAKPAAPAAPKPQAVAPYVSSGNEAELE
TREKMTPTRKAIAKAMLASKQRSPHVTSFDDVEVSKLMAHRKKYKQYAADKGIKLTFLPYIVKALVTVLREYPEFNASID
DTTDEIVYKHYFNIGIATNTDHGLYVPVIKNADAKSMFEIAKEISENAQKAYDSKLKPDEMRGGSMTISNVGSIGGGWFT
PVINQPEVAILGVGKIAKEPYVNEDNEIVVGNMLKLSLSYDHRLIDGALAQTALNLMDKLLADPDLLLMEG
>Mature_550_residues
AFEFKLPELGEGLAEGEIVKWDVKPGDDIKEDDTLLEVQSDKSVEEIPSPVSGKILKILVPEGETASVGDLLVEIDDGSG
PAAAPATATAAPATPAPATPAVQPAPAQSVYQFKLPELGEGLAEGEIVKWTVKPGDQIKEDDTLLEVQSDKSVEEIPSPV
AGTVVKILVPEGETATVGEALVDIDAPGHNDTSVATEAGAAPQPVAATPAATPAAPAAGGVTAITDPNREILAMPSVRQY
AREQGIDISQVPATGKHGRITKADVDAFKTGAPAASAQPAAPAAQAQPQPATAKPAAPAAPKPQAVAPYVSSGNEAELET
REKMTPTRKAIAKAMLASKQRSPHVTSFDDVEVSKLMAHRKKYKQYAADKGIKLTFLPYIVKALVTVLREYPEFNASIDD
TTDEIVYKHYFNIGIATNTDHGLYVPVIKNADAKSMFEIAKEISENAQKAYDSKLKPDEMRGGSMTISNVGSIGGGWFTP
VINQPEVAILGVGKIAKEPYVNEDNEIVVGNMLKLSLSYDHRLIDGALAQTALNLMDKLLADPDLLLMEG

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=440, Percent_Identity=33.8636363636364, Blast_Score=228, Evalue=9e-60,
Organism=Homo sapiens, GI31711992, Length=588, Percent_Identity=29.9319727891156, Blast_Score=193, Evalue=4e-49,
Organism=Homo sapiens, GI19923748, Length=254, Percent_Identity=37.007874015748, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI203098816, Length=482, Percent_Identity=29.045643153527, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=29.7117516629712, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI260898739, Length=141, Percent_Identity=38.2978723404255, Blast_Score=90, Evalue=6e-18,
Organism=Escherichia coli, GI1786305, Length=550, Percent_Identity=33.4545454545455, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1786946, Length=437, Percent_Identity=34.5537757437071, Blast_Score=214, Evalue=9e-57,
Organism=Caenorhabditis elegans, GI17537937, Length=442, Percent_Identity=32.3529411764706, Blast_Score=229, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI17560088, Length=464, Percent_Identity=29.7413793103448, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI25146366, Length=232, Percent_Identity=36.6379310344828, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17538894, Length=319, Percent_Identity=28.8401253918495, Blast_Score=114, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320352, Length=443, Percent_Identity=32.7313769751693, Blast_Score=198, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6324258, Length=230, Percent_Identity=29.1304347826087, Blast_Score=101, Evalue=3e-22,
Organism=Drosophila melanogaster, GI18859875, Length=439, Percent_Identity=31.6628701594533, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24582497, Length=232, Percent_Identity=34.051724137931, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI20129315, Length=232, Percent_Identity=34.051724137931, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24645909, Length=230, Percent_Identity=33.9130434782609, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 58079; Mature: 57948

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFEFKLPELGEGLAEGEIVKWDVKPGDDIKEDDTLLEVQSDKSVEEIPSPVSGKILKIL
CCEECCCCHHHCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCHHHCCCCCCCCEEEEE
VPEGETASVGDLLVEIDDGSGPAAAPATATAAPATPAPATPAVQPAPAQSVYQFKLPELG
ECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHC
EGLAEGEIVKWTVKPGDQIKEDDTLLEVQSDKSVEEIPSPVAGTVVKILVPEGETATVGE
CCCCCCCEEEEEECCCCCCCCCCCEEEECCCCCHHHCCCCHHCEEEEEEECCCCCCCCCC
ALVDIDAPGHNDTSVATEAGAAPQPVAATPAATPAAPAAGGVTAITDPNREILAMPSVRQ
EEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCHHHH
YAREQGIDISQVPATGKHGRITKADVDAFKTGAPAASAQPAAPAAQAQPQPATAKPAAPA
HHHHHCCCCCCCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APKPQAVAPYVSSGNEAELETREKMTPTRKAIAKAMLASKQRSPHVTSFDDVEVSKLMAH
CCCCCCCCCHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
RKKYKQYAADKGIKLTFLPYIVKALVTVLREYPEFNASIDDTTDEIVYKHYFNIGIATNT
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHEEEEEEECC
DHGLYVPVIKNADAKSMFEIAKEISENAQKAYDSKLKPDEMRGGSMTISNVGSIGGGWFT
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEECCCCCCCCCEEC
PVINQPEVAILGVGKIAKEPYVNEDNEIVVGNMLKLSLSYDHRLIDGALAQTALNLMDKL
CCCCCCCEEEEECCHHHCCCCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHH
LADPDLLLMEG
HCCCCEEEECC
>Mature Secondary Structure 
AFEFKLPELGEGLAEGEIVKWDVKPGDDIKEDDTLLEVQSDKSVEEIPSPVSGKILKIL
CEECCCCHHHCCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCHHHCCCCCCCCEEEEE
VPEGETASVGDLLVEIDDGSGPAAAPATATAAPATPAPATPAVQPAPAQSVYQFKLPELG
ECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHC
EGLAEGEIVKWTVKPGDQIKEDDTLLEVQSDKSVEEIPSPVAGTVVKILVPEGETATVGE
CCCCCCCEEEEEECCCCCCCCCCCEEEECCCCCHHHCCCCHHCEEEEEEECCCCCCCCCC
ALVDIDAPGHNDTSVATEAGAAPQPVAATPAATPAAPAAGGVTAITDPNREILAMPSVRQ
EEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCHHHH
YAREQGIDISQVPATGKHGRITKADVDAFKTGAPAASAQPAAPAAQAQPQPATAKPAAPA
HHHHHCCCCCCCCCCCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APKPQAVAPYVSSGNEAELETREKMTPTRKAIAKAMLASKQRSPHVTSFDDVEVSKLMAH
CCCCCCCCCHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
RKKYKQYAADKGIKLTFLPYIVKALVTVLREYPEFNASIDDTTDEIVYKHYFNIGIATNT
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHEEEEEEECC
DHGLYVPVIKNADAKSMFEIAKEISENAQKAYDSKLKPDEMRGGSMTISNVGSIGGGWFT
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEECCCCCCCCCEEC
PVINQPEVAILGVGKIAKEPYVNEDNEIVVGNMLKLSLSYDHRLIDGALAQTALNLMDKL
CCCCCCCEEEEECCHHHCCCCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHH
LADPDLLLMEG
HCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2200674; 3421911; 1915365; 8445635; 8450544; 8805537 [H]