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The map label for this gene is pdhD [H]

Identifier: 116494797

GI number: 116494797

Start: 1290987

End: 1292390

Strand: Direct

Name: pdhD [H]

Synonym: LSEI_1308

Alternate gene names: 116494797

Gene position: 1290987-1292390 (Clockwise)

Preceding gene: 116494796

Following gene: 116494798

Centisome position: 44.59

GC content: 49.29

Gene sequence:

>1404_bases
ATGGTTGTAGGCGATTTTGCAATCGATTTGGATACCGTCGTCATTGGTTCCGGCCCCGGCGGTTATGTTGCTGCGATTCG
CGCAGCTGAAATGGGTCAAAAGGTGACCGTGATTGAGAGCACGTTCATTGGCGGTGTTTGTTTGAATGTCGGCTGCATCC
CATCAAAGGCTTTGATCAACGCAGGTCATCGCTATCAAGACGCCTTGGAAGCAAGTACGTTTGGCATTAATGCCAAAGGT
GCTGACCTTGATTTCACGAAAACCCAGGAATGGAAGCAAAATAAGGTTGTGCATACGCTGACAAGCGGTGTGTCGATGCT
ATTCAAGAAACACAAGATTGACACGATTATGGGCACGGCGTTCTTAAAAGATGACCATAGCCTGCGCGTGATGCAAAAAG
ATTCAGCTCAGACCTATACGTTCAAGAATCTGATCATCGCGACTGGCAGCCGGCCAATCGAAATTAAAGGCTTTAAGTTT
GGCAAGCGCATTTTAGATTCAACAGGGGGTCTGAATCTTCCTGAAGTACCAAAAGAATTTGTCGTCATTGGCGGCGGCTA
TATCGGCTCCGAGTTGGCGAGTGCGTATGCTAATCTTGGCGCGCATGTCACGATTCTTGAAGGCACAAGTTCGATTCTGC
CAAACTTTGAGAAGGATATGGTTCAGCTAGTTCTGAATTCCTTCAAAAAACGTGGCGTAACAGTTATTACCAATGCGATG
GCTAAAGAAGCTGAAGACACTGGCAAAGGTGTCAAAGTGACGTACACGGCCGATGGTAAGGAACAGACGATTGCCGCCGA
TTATGTTATGGTGACGGTCGGACGGCGGCCAAATACTGACGATCTTGGTCTCGATATTGTCGGGGTCGAAACAACTGATC
GCGGTTTAATCAAGGTCGATGCTCAAGGCCGGACCAACAAACCGAATATCTATGCGATTGGCGACATTGTGCCAGGTGCA
GCATTGGCGCATAAGGCTAGCTATGAAGGTAAAGTCGCTGCTGAGGCCATCAGCGGCAAAGCCAGTGCGGTTGACTACAA
AGCGATGCCTGCCGTTTGCTTCACGGACCCTGAACTTGCAACAACTGGGATGACAGTTGCCGAGGCGAAGGATAAAGGGA
TCAAGGCTAAAGCCAGCAAGTTCCCATTTGCTGCCAATGGTCGTGCCCTCAGTCTTGCCCAGACAGAAGGGTTTGTCCGC
TTGGTCACTAATGAAAACGGTACGGTTATCGGCGGCCAAGTTGCTGGCGCCGGTGCTAGTGATTTGATTTCCGAACTGAC
CGTTGCTGTTGAAGGCGGTTTGAACGTTGAGGATCTTGCCTTGACCATTCATCCGCACCCAACACTGAGTGAAGTTATCA
TGGATGACGCTGAAGTTGCATTGGGACTCCCAATCAATATTTAG

Upstream 100 bases:

>100_bases
ATGACCATCGTTTGATTGACGGCGCGTTAGCACAAACCGCCTTGAATCTGATGGATAAATTGTTGGCAGATCCTGATCTG
CTGTTGATGGAGGGATAAGC

Downstream 100 bases:

>100_bases
TATCTGATAGAGATGTCATGAGGCAGTCTGATCTCCTTGATCAGGCTGTTTTTGTGTCATTTGGTTATCGCTAATGGTGA
ATGGGGCGTGCGGACAAATT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; Membrane-bound ribosome protein complex 50 kDa subunit [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MVVGDFAIDLDTVVIGSGPGGYVAAIRAAEMGQKVTVIESTFIGGVCLNVGCIPSKALINAGHRYQDALEASTFGINAKG
ADLDFTKTQEWKQNKVVHTLTSGVSMLFKKHKIDTIMGTAFLKDDHSLRVMQKDSAQTYTFKNLIIATGSRPIEIKGFKF
GKRILDSTGGLNLPEVPKEFVVIGGGYIGSELASAYANLGAHVTILEGTSSILPNFEKDMVQLVLNSFKKRGVTVITNAM
AKEAEDTGKGVKVTYTADGKEQTIAADYVMVTVGRRPNTDDLGLDIVGVETTDRGLIKVDAQGRTNKPNIYAIGDIVPGA
ALAHKASYEGKVAAEAISGKASAVDYKAMPAVCFTDPELATTGMTVAEAKDKGIKAKASKFPFAANGRALSLAQTEGFVR
LVTNENGTVIGGQVAGAGASDLISELTVAVEGGLNVEDLALTIHPHPTLSEVIMDDAEVALGLPINI

Sequences:

>Translated_467_residues
MVVGDFAIDLDTVVIGSGPGGYVAAIRAAEMGQKVTVIESTFIGGVCLNVGCIPSKALINAGHRYQDALEASTFGINAKG
ADLDFTKTQEWKQNKVVHTLTSGVSMLFKKHKIDTIMGTAFLKDDHSLRVMQKDSAQTYTFKNLIIATGSRPIEIKGFKF
GKRILDSTGGLNLPEVPKEFVVIGGGYIGSELASAYANLGAHVTILEGTSSILPNFEKDMVQLVLNSFKKRGVTVITNAM
AKEAEDTGKGVKVTYTADGKEQTIAADYVMVTVGRRPNTDDLGLDIVGVETTDRGLIKVDAQGRTNKPNIYAIGDIVPGA
ALAHKASYEGKVAAEAISGKASAVDYKAMPAVCFTDPELATTGMTVAEAKDKGIKAKASKFPFAANGRALSLAQTEGFVR
LVTNENGTVIGGQVAGAGASDLISELTVAVEGGLNVEDLALTIHPHPTLSEVIMDDAEVALGLPINI
>Mature_467_residues
MVVGDFAIDLDTVVIGSGPGGYVAAIRAAEMGQKVTVIESTFIGGVCLNVGCIPSKALINAGHRYQDALEASTFGINAKG
ADLDFTKTQEWKQNKVVHTLTSGVSMLFKKHKIDTIMGTAFLKDDHSLRVMQKDSAQTYTFKNLIIATGSRPIEIKGFKF
GKRILDSTGGLNLPEVPKEFVVIGGGYIGSELASAYANLGAHVTILEGTSSILPNFEKDMVQLVLNSFKKRGVTVITNAM
AKEAEDTGKGVKVTYTADGKEQTIAADYVMVTVGRRPNTDDLGLDIVGVETTDRGLIKVDAQGRTNKPNIYAIGDIVPGA
ALAHKASYEGKVAAEAISGKASAVDYKAMPAVCFTDPELATTGMTVAEAKDKGIKAKASKFPFAANGRALSLAQTEGFVR
LVTNENGTVIGGQVAGAGASDLISELTVAVEGGLNVEDLALTIHPHPTLSEVIMDDAEVALGLPINI

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential). Membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=479, Percent_Identity=39.4572025052192, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI50301238, Length=457, Percent_Identity=29.9781181619256, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI33519430, Length=454, Percent_Identity=26.8722466960352, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI33519428, Length=454, Percent_Identity=26.8722466960352, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI33519426, Length=454, Percent_Identity=26.8722466960352, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI148277065, Length=454, Percent_Identity=26.8722466960352, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI148277071, Length=454, Percent_Identity=26.8722466960352, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI291045266, Length=479, Percent_Identity=27.1398747390397, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI22035672, Length=464, Percent_Identity=27.801724137931, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI291045268, Length=471, Percent_Identity=26.963906581741, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1786307, Length=462, Percent_Identity=39.8268398268398, Blast_Score=315, Evalue=4e-87,
Organism=Escherichia coli, GI87081717, Length=450, Percent_Identity=30.2222222222222, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI87082354, Length=453, Percent_Identity=28.6975717439294, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1789915, Length=440, Percent_Identity=28.1818181818182, Blast_Score=163, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI32565766, Length=463, Percent_Identity=39.3088552915767, Blast_Score=301, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI17557007, Length=473, Percent_Identity=30.2325581395349, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI71983429, Length=449, Percent_Identity=27.8396436525612, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71983419, Length=449, Percent_Identity=27.8396436525612, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=477, Percent_Identity=24.5283018867925, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17559934, Length=220, Percent_Identity=28.6363636363636, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=461, Percent_Identity=36.6594360086768, Blast_Score=265, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6325166, Length=459, Percent_Identity=28.322440087146, Blast_Score=163, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6325240, Length=481, Percent_Identity=27.6507276507277, Blast_Score=162, Evalue=9e-41,
Organism=Drosophila melanogaster, GI21358499, Length=472, Percent_Identity=41.3135593220339, Blast_Score=316, Evalue=3e-86,
Organism=Drosophila melanogaster, GI24640549, Length=469, Percent_Identity=28.997867803838, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24640553, Length=469, Percent_Identity=28.997867803838, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24640551, Length=469, Percent_Identity=28.997867803838, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17737741, Length=476, Percent_Identity=25.8403361344538, Blast_Score=125, Evalue=6e-29,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49078; Mature: 49078

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVGDFAIDLDTVVIGSGPGGYVAAIRAAEMGQKVTVIESTFIGGVCLNVGCIPSKALIN
CEEEEEEEEEEEEEEECCCCCEEEEEEHHHCCCEEEEEEHHEECCEEEEECCCCCHHHHC
AGHRYQDALEASTFGINAKGADLDFTKTQEWKQNKVVHTLTSGVSMLFKKHKIDTIMGTA
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEE
FLKDDHSLRVMQKDSAQTYTFKNLIIATGSRPIEIKGFKFGKRILDSTGGLNLPEVPKEF
EECCCCCEEEEECCCCCEEEEEEEEEECCCCEEEEECCHHHHHHHHCCCCCCCCCCCCEE
VVIGGGYIGSELASAYANLGAHVTILEGTSSILPNFEKDMVQLVLNSFKKRGVTVITNAM
EEEECCHHHHHHHHHHHCCCCEEEEEECCHHHCCCHHHHHHHHHHHHHHHCCEEEEEHHH
AKEAEDTGKGVKVTYTADGKEQTIAADYVMVTVGRRPNTDDLGLDIVGVETTDRGLIKVD
HHHHHHCCCCEEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCEEEEEEEECCCCEEEEE
AQGRTNKPNIYAIGDIVPGAALAHKASYEGKVAAEAISGKASAVDYKAMPAVCFTDPELA
CCCCCCCCCEEEECCCCCCHHHHHCCCCCCCEEHHHHCCCCCEECCCCCCEEEECCCCHH
TTGMTVAEAKDKGIKAKASKFPFAANGRALSLAQTEGFVRLVTNENGTVIGGQVAGAGAS
HCCCEEECCCCCCCEEHHCCCCCCCCCCEEEEEECCCEEEEEECCCCCEEECEECCCCHH
DLISELTVAVEGGLNVEDLALTIHPHPTLSEVIMDDAEVALGLPINI
HHHHHHEEEEECCCCEEEEEEEECCCCCHHHHHHCCCCEEEECCCCC
>Mature Secondary Structure
MVVGDFAIDLDTVVIGSGPGGYVAAIRAAEMGQKVTVIESTFIGGVCLNVGCIPSKALIN
CEEEEEEEEEEEEEEECCCCCEEEEEEHHHCCCEEEEEEHHEECCEEEEECCCCCHHHHC
AGHRYQDALEASTFGINAKGADLDFTKTQEWKQNKVVHTLTSGVSMLFKKHKIDTIMGTA
CCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEE
FLKDDHSLRVMQKDSAQTYTFKNLIIATGSRPIEIKGFKFGKRILDSTGGLNLPEVPKEF
EECCCCCEEEEECCCCCEEEEEEEEEECCCCEEEEECCHHHHHHHHCCCCCCCCCCCCEE
VVIGGGYIGSELASAYANLGAHVTILEGTSSILPNFEKDMVQLVLNSFKKRGVTVITNAM
EEEECCHHHHHHHHHHHCCCCEEEEEECCHHHCCCHHHHHHHHHHHHHHHCCEEEEEHHH
AKEAEDTGKGVKVTYTADGKEQTIAADYVMVTVGRRPNTDDLGLDIVGVETTDRGLIKVD
HHHHHHCCCCEEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCEEEEEEEECCCCEEEEE
AQGRTNKPNIYAIGDIVPGAALAHKASYEGKVAAEAISGKASAVDYKAMPAVCFTDPELA
CCCCCCCCCEEEECCCCCCHHHHHCCCCCCCEEHHHHCCCCCEECCCCCCEEEECCCCHH
TTGMTVAEAKDKGIKAKASKFPFAANGRALSLAQTEGFVRLVTNENGTVIGGQVAGAGAS
HCCCEEECCCCCCCEEHHCCCCCCCCCCEEEEEECCCEEEEEECCCCCEEECEECCCCHH
DLISELTVAVEGGLNVEDLALTIHPHPTLSEVIMDDAEVALGLPINI
HHHHHHEEEEECCCCEEEEEEEECCCCCHHHHHHCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA