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The map label for this gene is gatA

Identifier: 116494560

GI number: 116494560

Start: 1040850

End: 1042304

Strand: Direct

Name: gatA

Synonym: LSEI_1058

Alternate gene names: 116494560

Gene position: 1040850-1042304 (Clockwise)

Preceding gene: 116494559

Following gene: 116494561

Centisome position: 35.95

GC content: 50.58

Gene sequence:

>1455_bases
GTGGATTACTTTAAAACCAGTATCGATCAATTGCATGAGCAGTTACGCTCCGGCAAGGTGACCAGCAGTCAATTGGTTGA
CGAGACATTGGCCGGCATCAACCAGTTGGATGCTGAAGTAGATGCCTTCTTAGCGCTGAATGCTGATGGCGCCAAGGAAA
AGGCTGCTGCAGTAGATGCAGCCGGTATTGCTGACGATCAACCACTAGCCGGTGTGCCAATTGCTATCAAAGACAACATT
GTCACCAAAGGCGTTGTCACGACGGCAGCGTCCAAGATACTTGCCAACTTCAATCCGATTTATGATGCGACCGTTATACA
AAAGCTTGATGCAGCCGGTGCGATCAACGTTGGTAAAACCAACATGGACGAATTCGCAATGGGCTCGTCAACAGAAAACT
CCGCTTTTAAAACGACTAAAAACGCTTGGGATCACACGCGCGTTCCCGGTGGGTCGTCAGGCGGCTCCGCGGCAGCTGTC
GCAGCTGGTGAAGTGATTGCCGCACTGGGCAGTGATACAGGCGGTTCCATTCGTCAGCCTGCTGCTTTTAACGGCATCGT
CGGGGTCAAACCGACATACGGCCGCGTTTCTCGCTGGGGTTTGATTGCCTTTAGTTCCAGCCTTGATCAGATCGGAACGC
TGACTCGACATGTTAAAGACGCTGCGCAGTTGCTGAATGTGATTGCTGGTCATGACGAACGTGATAGCACCACAGCGGAC
ACACCCGTACCGGACTTTACCGCCAAAATCGGTCAGGATATCAAGGGGATGAAGATCGCTTTGCCAAAGGAATACTTAGG
CAAAGGGGTTGACCCAGCGGTTGCGGACAAGATTAAAGCAGCTGCCAAGCAGTTCGAAGACATGGGCGCGACGGTGACAG
AAGTGTCACTGCCACATACTCAATATGCGGTGCCATCTTACTACATCATCGCCAGCTCCGAAGCCAGCTCCAATCTGCAG
CGCTTTGATGGCATTCGTTATGGTTTCCGCGCCAAGGATGTCAAAAATATTGAGGATGTTTATGTTCGTTCTCGATCCGA
AGGTTTTGGCCCAGAAGTCAAGCGACGTATCATGCTGGGCACCTTCAGTTTGTCAGCCGGCTTTTATGATGCATACTTCA
AAAAGGCAGGGCAGGTTCGAACTTTGATTACCCGTGACTTTGAAGATGTCTTCAAAGATTATGATCTGATTATTGGACCG
ACAACCCCAACCGTTGCGTTCAAAATTGGCGAAAAAGTCACCGATCCAGTTACCATGTACATGAACGACATTTTGACAAT
TCCGGTCAATCTTGCCGGCTTACCGGCGGCATCCGTGCCAGCTGGTTTCGTTGATGGCATGCCAGTCGGCTTACAGCTGA
TCGGCAAACATTTTGACGAAAGCACGATCTTCCAAGTTGCTGCGGCTTTTGAAGCACAAAACGACTACTTAGCACAGATC
CCGGGGGGCAAGTAA

Upstream 100 bases:

>100_bases
GCCAGGCGAACCGGTAGCGGACTTGTTGAAAAATGTGCCAACCAAGAAGGGCACGTTGATCCAAGTTCCAGCGATCATCG
ATAAAGAGGAGGACTAGCGC

Downstream 100 bases:

>100_bases
GATGAACTTTGAAACAACAATCGGGCTTGAAGTCCACGTTGAGTTGAAAACCAAATCGAAAATGTTCTCACCATCACCTG
TCACTTATGGTCAGGAACCC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MDYFKTSIDQLHEQLRSGKVTSSQLVDETLAGINQLDAEVDAFLALNADGAKEKAAAVDAAGIADDQPLAGVPIAIKDNI
VTKGVVTTAASKILANFNPIYDATVIQKLDAAGAINVGKTNMDEFAMGSSTENSAFKTTKNAWDHTRVPGGSSGGSAAAV
AAGEVIAALGSDTGGSIRQPAAFNGIVGVKPTYGRVSRWGLIAFSSSLDQIGTLTRHVKDAAQLLNVIAGHDERDSTTAD
TPVPDFTAKIGQDIKGMKIALPKEYLGKGVDPAVADKIKAAAKQFEDMGATVTEVSLPHTQYAVPSYYIIASSEASSNLQ
RFDGIRYGFRAKDVKNIEDVYVRSRSEGFGPEVKRRIMLGTFSLSAGFYDAYFKKAGQVRTLITRDFEDVFKDYDLIIGP
TTPTVAFKIGEKVTDPVTMYMNDILTIPVNLAGLPAASVPAGFVDGMPVGLQLIGKHFDESTIFQVAAAFEAQNDYLAQI
PGGK

Sequences:

>Translated_484_residues
MDYFKTSIDQLHEQLRSGKVTSSQLVDETLAGINQLDAEVDAFLALNADGAKEKAAAVDAAGIADDQPLAGVPIAIKDNI
VTKGVVTTAASKILANFNPIYDATVIQKLDAAGAINVGKTNMDEFAMGSSTENSAFKTTKNAWDHTRVPGGSSGGSAAAV
AAGEVIAALGSDTGGSIRQPAAFNGIVGVKPTYGRVSRWGLIAFSSSLDQIGTLTRHVKDAAQLLNVIAGHDERDSTTAD
TPVPDFTAKIGQDIKGMKIALPKEYLGKGVDPAVADKIKAAAKQFEDMGATVTEVSLPHTQYAVPSYYIIASSEASSNLQ
RFDGIRYGFRAKDVKNIEDVYVRSRSEGFGPEVKRRIMLGTFSLSAGFYDAYFKKAGQVRTLITRDFEDVFKDYDLIIGP
TTPTVAFKIGEKVTDPVTMYMNDILTIPVNLAGLPAASVPAGFVDGMPVGLQLIGKHFDESTIFQVAAAFEAQNDYLAQI
PGGK
>Mature_484_residues
MDYFKTSIDQLHEQLRSGKVTSSQLVDETLAGINQLDAEVDAFLALNADGAKEKAAAVDAAGIADDQPLAGVPIAIKDNI
VTKGVVTTAASKILANFNPIYDATVIQKLDAAGAINVGKTNMDEFAMGSSTENSAFKTTKNAWDHTRVPGGSSGGSAAAV
AAGEVIAALGSDTGGSIRQPAAFNGIVGVKPTYGRVSRWGLIAFSSSLDQIGTLTRHVKDAAQLLNVIAGHDERDSTTAD
TPVPDFTAKIGQDIKGMKIALPKEYLGKGVDPAVADKIKAAAKQFEDMGATVTEVSLPHTQYAVPSYYIIASSEASSNLQ
RFDGIRYGFRAKDVKNIEDVYVRSRSEGFGPEVKRRIMLGTFSLSAGFYDAYFKKAGQVRTLITRDFEDVFKDYDLIIGP
TTPTVAFKIGEKVTDPVTMYMNDILTIPVNLAGLPAASVPAGFVDGMPVGLQLIGKHFDESTIFQVAAAFEAQNDYLAQI
PGGK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=497, Percent_Identity=38.8329979879276, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI195972892, Length=482, Percent_Identity=24.896265560166, Blast_Score=132, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17543272, Length=405, Percent_Identity=37.037037037037, Blast_Score=256, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI17537465, Length=492, Percent_Identity=24.7967479674797, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17556264, Length=195, Percent_Identity=34.8717948717949, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17538252, Length=451, Percent_Identity=23.059866962306, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71990152, Length=136, Percent_Identity=37.5, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=250, Percent_Identity=30.8, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=250, Percent_Identity=30.8, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=199, Percent_Identity=30.1507537688442, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=420, Percent_Identity=35.7142857142857, Blast_Score=245, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=412, Percent_Identity=32.7669902912621, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320448, Length=132, Percent_Identity=35.6060606060606, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648113, Length=494, Percent_Identity=41.497975708502, Blast_Score=322, Evalue=4e-88,
Organism=Drosophila melanogaster, GI24652985, Length=494, Percent_Identity=26.3157894736842, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI19922090, Length=494, Percent_Identity=26.3157894736842, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24652981, Length=494, Percent_Identity=26.3157894736842, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24652983, Length=494, Percent_Identity=26.3157894736842, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24644968, Length=501, Percent_Identity=26.9461077844311, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI161078093, Length=501, Percent_Identity=26.7465069860279, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI45550774, Length=239, Percent_Identity=31.3807531380753, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648435, Length=239, Percent_Identity=31.3807531380753, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648437, Length=239, Percent_Identity=31.3807531380753, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648441, Length=173, Percent_Identity=35.2601156069364, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648439, Length=173, Percent_Identity=35.2601156069364, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21356731, Length=499, Percent_Identity=25.6513026052104, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_LACC3 (Q03AC0)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806294.1
- ProteinModelPortal:   Q03AC0
- SMR:   Q03AC0
- STRING:   Q03AC0
- GeneID:   4418843
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_1058
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   Q03AC0
- ProtClustDB:   PRK00012
- BioCyc:   LCAS321967:LSEI_1058-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51333; Mature: 51333

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 77-77 ACT_SITE 152-152 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYFKTSIDQLHEQLRSGKVTSSQLVDETLAGINQLDAEVDAFLALNADGAKEKAAAVDA
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHH
AGIADDQPLAGVPIAIKDNIVTKGVVTTAASKILANFNPIYDATVIQKLDAAGAINVGKT
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEECCCC
NMDEFAMGSSTENSAFKTTKNAWDHTRVPGGSSGGSAAAVAAGEVIAALGSDTGGSIRQP
CHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
AAFNGIVGVKPTYGRVSRWGLIAFSSSLDQIGTLTRHVKDAAQLLNVIAGHDERDSTTAD
CCCCCCCCCCCCCCCHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TPVPDFTAKIGQDIKGMKIALPKEYLGKGVDPAVADKIKAAAKQFEDMGATVTEVSLPHT
CCCCHHHHHHCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCC
QYAVPSYYIIASSEASSNLQRFDGIRYGFRAKDVKNIEDVYVRSRSEGFGPEVKRRIMLG
CCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHEEEEE
TFSLSAGFYDAYFKKAGQVRTLITRDFEDVFKDYDLIIGPTTPTVAFKIGEKVTDPVTMY
EEECCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCEEEEECCCCCCCHHHHH
MNDILTIPVNLAGLPAASVPAGFVDGMPVGLQLIGKHFDESTIFQVAAAFEAQNDYLAQI
HHCCEEECEECCCCCCCCCCCCHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEC
PGGK
CCCC
>Mature Secondary Structure
MDYFKTSIDQLHEQLRSGKVTSSQLVDETLAGINQLDAEVDAFLALNADGAKEKAAAVDA
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHH
AGIADDQPLAGVPIAIKDNIVTKGVVTTAASKILANFNPIYDATVIQKLDAAGAINVGKT
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEECCCC
NMDEFAMGSSTENSAFKTTKNAWDHTRVPGGSSGGSAAAVAAGEVIAALGSDTGGSIRQP
CHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
AAFNGIVGVKPTYGRVSRWGLIAFSSSLDQIGTLTRHVKDAAQLLNVIAGHDERDSTTAD
CCCCCCCCCCCCCCCHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TPVPDFTAKIGQDIKGMKIALPKEYLGKGVDPAVADKIKAAAKQFEDMGATVTEVSLPHT
CCCCHHHHHHCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCC
QYAVPSYYIIASSEASSNLQRFDGIRYGFRAKDVKNIEDVYVRSRSEGFGPEVKRRIMLG
CCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHEEEEE
TFSLSAGFYDAYFKKAGQVRTLITRDFEDVFKDYDLIIGPTTPTVAFKIGEKVTDPVTMY
EEECCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCEEEEECCCCCCCHHHHH
MNDILTIPVNLAGLPAASVPAGFVDGMPVGLQLIGKHFDESTIFQVAAAFEAQNDYLAQI
HHCCEEECEECCCCCCCCCCCCHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEC
PGGK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA