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The map label for this gene is gatB

Identifier: 116494561

GI number: 116494561

Start: 1042306

End: 1043736

Strand: Direct

Name: gatB

Synonym: LSEI_1059

Alternate gene names: 116494561

Gene position: 1042306-1043736 (Clockwise)

Preceding gene: 116494560

Following gene: 116494562

Centisome position: 36.0

GC content: 47.94

Gene sequence:

>1431_bases
ATGAACTTTGAAACAACAATCGGGCTTGAAGTCCACGTTGAGTTGAAAACCAAATCGAAAATGTTCTCACCATCACCTGT
CACTTATGGTCAGGAACCCAACACCCAGACAAACGTGATTGACTGGGGATTCCCAGGCGTTTTGCCAAGCATCAATCGCG
GGGCCTATCAACTTGGCATCATGGTGGGGCTGGCATTGCATGCTGACATCACGCGTTTGACTCATTTTGATCGGAAAAAC
TATTTCTATCCTGACAACCCTAAGGCTTATCAAATCACCCAAAGTGAGAAGCCTTTGGGCACCAATGGCTGGGTGGAAAT
TGAAGTTGACGGTAAGAAGAAAAAGATTGGTATCGCAGAACTGCACGTTGAAGAAGATGCCGGGAAGAACCAGCACGAAG
ACGATGGTTATTCCTATGTTGATTTGAACCGTCAAGGCACACCGCTGGTCGAAATTGTTTCCAAACCGGATATCACCAGT
CCAGAAGAAGCTTATGCTTATCTGGAAACCTTGCGCCAAATCGTTCAGTTCACAGGGGCTTCGGACGTTAAGATGGAAGA
AGGCTCTATGCGGGTTGATACCAATTTGTCAGTGCGGCCAATTGGACAGGAACATTTTGGTATCAAGACTGAAATCAAGA
ACCTGAACTCCTTCGTGCATGTTCGCGATGGGCTCGCATTTGAAGAAAAACGGCAGCAGGCTGTGCTTTTGAGCGGCGGT
GAAGTGCGTCAGGAAACCCGGCGCTGGGATCCAGATGCTAAAGAAACGCTTCTGATGCGGGTTAAGGAAGGCGCCGATGA
TTATCGTTATTTCCCTGAACCAGACCTGCCACCAGTGGCTGTTTCCCAGAAATGGATTGACGACATTCAGGCCTCGTTAC
CGCAACCGCCAGCAGAGCGGCGGCAGCGTTACATCGAAGACTGGGGAATCCCAGCTTATGATGCCGGTGTCTTGACGCAG
ACGAAGGAAATGTCAGATTTCTTCGAAGCAACTGTGGCACAAGGCGCTGATGCCAAGCAAGCGTCTAACTGGCTGATGGG
TGAGGTTTCCGGTTTCTTGAATGCCCAACACGTTGAACTGGGGCAAGTTGCCTTGACGCCAGCGCATTTGGCAGGCATGA
TCAAGCTTATTGGTGATGGCACGATTTCCAGTAAAATGGCCAAAAAGGTCTTTAAGGAAATCATTCAACATGATACTGAT
CCGGATAAGTGGGTCCATGAAAAAGGTCTGATCCAACTTTCCGATCCGGCTAAATTGACCCCGATTATTGACGAAATTCT
TGATAATAACCAGCAAAGTATCGATGACTTCAAAGCTGGTAAGGACCGGGCAATTGGCTTCCTAGTTGGTCAGATCATGA
AACAAACACATGGTCAGGCAAACCCTAAAGTGGTCAACCAGATTTTGATGGCTGAGATTAAGCAACGATAA

Upstream 100 bases:

>100_bases
CTGATCGGCAAACATTTTGACGAAAGCACGATCTTCCAAGTTGCTGCGGCTTTTGAAGCACAAAACGACTACTTAGCACA
GATCCCGGGGGGCAAGTAAG

Downstream 100 bases:

>100_bases
GTGATACTGACTATCTAGCAATTCAGTTCGGATAATAGCCTCGACATGTAGACAATGTTCTTTGTTGTTTACATGCCGAG
GTTATTGCGCAAATTGCTAG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MNFETTIGLEVHVELKTKSKMFSPSPVTYGQEPNTQTNVIDWGFPGVLPSINRGAYQLGIMVGLALHADITRLTHFDRKN
YFYPDNPKAYQITQSEKPLGTNGWVEIEVDGKKKKIGIAELHVEEDAGKNQHEDDGYSYVDLNRQGTPLVEIVSKPDITS
PEEAYAYLETLRQIVQFTGASDVKMEEGSMRVDTNLSVRPIGQEHFGIKTEIKNLNSFVHVRDGLAFEEKRQQAVLLSGG
EVRQETRRWDPDAKETLLMRVKEGADDYRYFPEPDLPPVAVSQKWIDDIQASLPQPPAERRQRYIEDWGIPAYDAGVLTQ
TKEMSDFFEATVAQGADAKQASNWLMGEVSGFLNAQHVELGQVALTPAHLAGMIKLIGDGTISSKMAKKVFKEIIQHDTD
PDKWVHEKGLIQLSDPAKLTPIIDEILDNNQQSIDDFKAGKDRAIGFLVGQIMKQTHGQANPKVVNQILMAEIKQR

Sequences:

>Translated_476_residues
MNFETTIGLEVHVELKTKSKMFSPSPVTYGQEPNTQTNVIDWGFPGVLPSINRGAYQLGIMVGLALHADITRLTHFDRKN
YFYPDNPKAYQITQSEKPLGTNGWVEIEVDGKKKKIGIAELHVEEDAGKNQHEDDGYSYVDLNRQGTPLVEIVSKPDITS
PEEAYAYLETLRQIVQFTGASDVKMEEGSMRVDTNLSVRPIGQEHFGIKTEIKNLNSFVHVRDGLAFEEKRQQAVLLSGG
EVRQETRRWDPDAKETLLMRVKEGADDYRYFPEPDLPPVAVSQKWIDDIQASLPQPPAERRQRYIEDWGIPAYDAGVLTQ
TKEMSDFFEATVAQGADAKQASNWLMGEVSGFLNAQHVELGQVALTPAHLAGMIKLIGDGTISSKMAKKVFKEIIQHDTD
PDKWVHEKGLIQLSDPAKLTPIIDEILDNNQQSIDDFKAGKDRAIGFLVGQIMKQTHGQANPKVVNQILMAEIKQR
>Mature_476_residues
MNFETTIGLEVHVELKTKSKMFSPSPVTYGQEPNTQTNVIDWGFPGVLPSINRGAYQLGIMVGLALHADITRLTHFDRKN
YFYPDNPKAYQITQSEKPLGTNGWVEIEVDGKKKKIGIAELHVEEDAGKNQHEDDGYSYVDLNRQGTPLVEIVSKPDITS
PEEAYAYLETLRQIVQFTGASDVKMEEGSMRVDTNLSVRPIGQEHFGIKTEIKNLNSFVHVRDGLAFEEKRQQAVLLSGG
EVRQETRRWDPDAKETLLMRVKEGADDYRYFPEPDLPPVAVSQKWIDDIQASLPQPPAERRQRYIEDWGIPAYDAGVLTQ
TKEMSDFFEATVAQGADAKQASNWLMGEVSGFLNAQHVELGQVALTPAHLAGMIKLIGDGTISSKMAKKVFKEIIQHDTD
PDKWVHEKGLIQLSDPAKLTPIIDEILDNNQQSIDDFKAGKDRAIGFLVGQIMKQTHGQANPKVVNQILMAEIKQR

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=488, Percent_Identity=33.8114754098361, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI115534094, Length=346, Percent_Identity=35.2601156069364, Blast_Score=203, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6319391, Length=506, Percent_Identity=28.8537549407115, Blast_Score=213, Evalue=6e-56,
Organism=Drosophila melanogaster, GI45550808, Length=489, Percent_Identity=30.879345603272, Blast_Score=253, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_LACC3 (Q03AB9)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806295.1
- ProteinModelPortal:   Q03AB9
- SMR:   Q03AB9
- STRING:   Q03AB9
- GeneID:   4418844
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_1059
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   RIHGATH
- PhylomeDB:   Q03AB9
- ProtClustDB:   PRK05477
- BioCyc:   LCAS321967:LSEI_1059-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53366; Mature: 53366

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFETTIGLEVHVELKTKSKMFSPSPVTYGQEPNTQTNVIDWGFPGVLPSINRGAYQLGI
CCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHH
MVGLALHADITRLTHFDRKNYFYPDNPKAYQITQSEKPLGTNGWVEIEVDGKKKKIGIAE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCEEE
LHVEEDAGKNQHEDDGYSYVDLNRQGTPLVEIVSKPDITSPEEAYAYLETLRQIVQFTGA
EEEECCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
SDVKMEEGSMRVDTNLSVRPIGQEHFGIKTEIKNLNSFVHVRDGLAFEEKRQQAVLLSGG
CCCEECCCCEEECCCCEEEECCHHHCCCHHHHHHHHHEEEECCCCCHHHHCCCEEEECCC
EVRQETRRWDPDAKETLLMRVKEGADDYRYFPEPDLPPVAVSQKWIDDIQASLPQPPAER
HHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
RQRYIEDWGIPAYDAGVLTQTKEMSDFFEATVAQGADAKQASNWLMGEVSGFLNAQHVEL
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEC
GQVALTPAHLAGMIKLIGDGTISSKMAKKVFKEIIQHDTDPDKWVHEKGLIQLSDPAKLT
CCEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCHH
PIIDEILDNNQQSIDDFKAGKDRAIGFLVGQIMKQTHGQANPKVVNQILMAEIKQR
HHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFETTIGLEVHVELKTKSKMFSPSPVTYGQEPNTQTNVIDWGFPGVLPSINRGAYQLGI
CCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHH
MVGLALHADITRLTHFDRKNYFYPDNPKAYQITQSEKPLGTNGWVEIEVDGKKKKIGIAE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCEEE
LHVEEDAGKNQHEDDGYSYVDLNRQGTPLVEIVSKPDITSPEEAYAYLETLRQIVQFTGA
EEEECCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
SDVKMEEGSMRVDTNLSVRPIGQEHFGIKTEIKNLNSFVHVRDGLAFEEKRQQAVLLSGG
CCCEECCCCEEECCCCEEEECCHHHCCCHHHHHHHHHEEEECCCCCHHHHCCCEEEECCC
EVRQETRRWDPDAKETLLMRVKEGADDYRYFPEPDLPPVAVSQKWIDDIQASLPQPPAER
HHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
RQRYIEDWGIPAYDAGVLTQTKEMSDFFEATVAQGADAKQASNWLMGEVSGFLNAQHVEL
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEC
GQVALTPAHLAGMIKLIGDGTISSKMAKKVFKEIIQHDTDPDKWVHEKGLIQLSDPAKLT
CCEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCHH
PIIDEILDNNQQSIDDFKAGKDRAIGFLVGQIMKQTHGQANPKVVNQILMAEIKQR
HHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA