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The map label for this gene is vanS [H]

Identifier: 116493791

GI number: 116493791

Start: 214685

End: 215875

Strand: Direct

Name: vanS [H]

Synonym: LSEI_0220

Alternate gene names: 116493791

Gene position: 214685-215875 (Clockwise)

Preceding gene: 116493790

Following gene: 116493792

Centisome position: 7.42

GC content: 44.67

Gene sequence:

>1191_bases
ATGAGCGAAAAAGTCAAACTAACTGGTAAGGAAAAATCCGAACTTTGGATTGAAGGCATCGTAACGGTGATTCTGCTGTT
AATGCTTAATTTTGCCTTGCTGGTGTTGATTAATCAGATGATCGCGCACAATCCTGGTTTGGAAAATGCTATCTGGGGTG
TGAAAACGAATCTGACGTTTGGCAGCCGTGGATTTCATTTGTGGAGCTGGTCCAATCTTTTCCTAGCACTGATGGCGATT
GCTGATGTCATTGTGGTGTATTGGCGACTTGCTCGGCGATATCGGCAAATGCAAATGCGTCACGTTATTGCGGAGCTGCA
TTTTATTGCCGATGGTCATCTTGATCATCGGATTAAGTTTGAGGTCAATACGGAGTTGCAAAAGGTTGTCAGCAGTATTA
ACGCGCTTGTTGATTCAACCGTCAATTCAATGGCCGAGGAACGGCGGATCGAACAGAGTAAAGATGAGCTGATTACTAAT
GTCAGCCATGATATTCGAACGCCGCTAACGTCCATTATCGGTTATCTTGGCCTGATTGAAGATCATCAATATCGCAGTGA
AGAAGAGCTACTCAAGTACACGCACACGGCTTATTTGAAGGCTAAGCAGATGAAAGTACTGGTTGAGGACCTTTTTGAGT
ACACCAAGGTTAAATCGACCACGACCAAGCTGAATCCCGTACGTTTTAACATGATTGCGATGCTAGAGCAACTGGCAGCT
AGTTTTGAGCTGGAGGCGCATAAGAAGCATATGCGGATTCTGGTCAGCGGTGAACCTGACCCGTTGATGATGGAAGCTGA
TACCGAAAAATTAGGCCGGGTGTTCAACAATTTAATTGTTAATGCGCTTAAGTACGGCCAAGGCGGGCGTCACATTTTTC
TTGATGCACAACGTATCGGTAGTGAAGTCGTTGTTAAGGTCTCAAATGATGGCGCACAAATTCCGAATGATGCTTTGAGT
CAGTTGTTTGATCGTTTTTATCGCGTTGAGGAAAGCCGATCGCAGGAAACTGGCGGGACTGGTCTTGGGCTAGCGATTGC
CCAAAGTATCATCGCTTTGCATGGCGGGTATATTTATGCCGATTCTAGCCCGGAGATAACAAGCTTTGTGATTCATTTAC
CATTAAAAGTTGGCGCTTCGCTGACAGACAATAAAAATAAATCTAATGAGGAAGCAGGAAAGTCAAAATGA

Upstream 100 bases:

>100_bases
ATGTCTCGCATCTGCGTGACAAGATTGAAGAAGCGACCGATGGCGAGAAGGTGATCGAAACAGTTTGGGGCGTCGGCTAT
AAGGTTGAGGCCTAACGATC

Downstream 100 bases:

>100_bases
AAAAGTTGATGCGCGGGTTGATTGCCGCAGTGGTGGCGGTATTAATGATTGGGGGTGCTGCGCCAGCAATGTCGGTATCT
GCGGCAGAGAGCAGCGATAC

Product: Signal transduction histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSEKVKLTGKEKSELWIEGIVTVILLLMLNFALLVLINQMIAHNPGLENAIWGVKTNLTFGSRGFHLWSWSNLFLALMAI
ADVIVVYWRLARRYRQMQMRHVIAELHFIADGHLDHRIKFEVNTELQKVVSSINALVDSTVNSMAEERRIEQSKDELITN
VSHDIRTPLTSIIGYLGLIEDHQYRSEEELLKYTHTAYLKAKQMKVLVEDLFEYTKVKSTTTKLNPVRFNMIAMLEQLAA
SFELEAHKKHMRILVSGEPDPLMMEADTEKLGRVFNNLIVNALKYGQGGRHIFLDAQRIGSEVVVKVSNDGAQIPNDALS
QLFDRFYRVEESRSQETGGTGLGLAIAQSIIALHGGYIYADSSPEITSFVIHLPLKVGASLTDNKNKSNEEAGKSK

Sequences:

>Translated_396_residues
MSEKVKLTGKEKSELWIEGIVTVILLLMLNFALLVLINQMIAHNPGLENAIWGVKTNLTFGSRGFHLWSWSNLFLALMAI
ADVIVVYWRLARRYRQMQMRHVIAELHFIADGHLDHRIKFEVNTELQKVVSSINALVDSTVNSMAEERRIEQSKDELITN
VSHDIRTPLTSIIGYLGLIEDHQYRSEEELLKYTHTAYLKAKQMKVLVEDLFEYTKVKSTTTKLNPVRFNMIAMLEQLAA
SFELEAHKKHMRILVSGEPDPLMMEADTEKLGRVFNNLIVNALKYGQGGRHIFLDAQRIGSEVVVKVSNDGAQIPNDALS
QLFDRFYRVEESRSQETGGTGLGLAIAQSIIALHGGYIYADSSPEITSFVIHLPLKVGASLTDNKNKSNEEAGKSK
>Mature_395_residues
SEKVKLTGKEKSELWIEGIVTVILLLMLNFALLVLINQMIAHNPGLENAIWGVKTNLTFGSRGFHLWSWSNLFLALMAIA
DVIVVYWRLARRYRQMQMRHVIAELHFIADGHLDHRIKFEVNTELQKVVSSINALVDSTVNSMAEERRIEQSKDELITNV
SHDIRTPLTSIIGYLGLIEDHQYRSEEELLKYTHTAYLKAKQMKVLVEDLFEYTKVKSTTTKLNPVRFNMIAMLEQLAAS
FELEAHKKHMRILVSGEPDPLMMEADTEKLGRVFNNLIVNALKYGQGGRHIFLDAQRIGSEVVVKVSNDGAQIPNDALSQ
LFDRFYRVEESRSQETGGTGLGLAIAQSIIALHGGYIYADSSPEITSFVIHLPLKVGASLTDNKNKSNEEAGKSK

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=281, Percent_Identity=28.4697508896797, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1786783, Length=318, Percent_Identity=29.559748427673, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693157, Length=227, Percent_Identity=29.5154185022026, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI87081816, Length=348, Percent_Identity=23.2758620689655, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789149, Length=374, Percent_Identity=22.7272727272727, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1786600, Length=237, Percent_Identity=28.2700421940928, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787894, Length=292, Percent_Identity=27.0547945205479, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790346, Length=275, Percent_Identity=27.6363636363636, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994928, Length=234, Percent_Identity=29.9145299145299, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788713, Length=232, Percent_Identity=26.7241379310345, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1786912, Length=264, Percent_Identity=26.8939393939394, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87082128, Length=230, Percent_Identity=25.2173913043478, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790436, Length=224, Percent_Identity=27.6785714285714, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788549, Length=236, Percent_Identity=23.728813559322, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790861, Length=204, Percent_Identity=22.5490196078431, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44707; Mature: 44576

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKVKLTGKEKSELWIEGIVTVILLLMLNFALLVLINQMIAHNPGLENAIWGVKTNLTF
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCEE
GSRGFHLWSWSNLFLALMAIADVIVVYWRLARRYRQMQMRHVIAELHFIADGHLDHRIKF
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
EVNTELQKVVSSINALVDSTVNSMAEERRIEQSKDELITNVSHDIRTPLTSIIGYLGLIE
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHQYRSEEELLKYTHTAYLKAKQMKVLVEDLFEYTKVKSTTTKLNPVRFNMIAMLEQLAA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SFELEAHKKHMRILVSGEPDPLMMEADTEKLGRVFNNLIVNALKYGQGGRHIFLDAQRIG
HHHHHHHHCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCC
SEVVVKVSNDGAQIPNDALSQLFDRFYRVEESRSQETGGTGLGLAIAQSIIALHGGYIYA
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEE
DSSPEITSFVIHLPLKVGASLTDNKNKSNEEAGKSK
CCCCCHHHHHEEEHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEKVKLTGKEKSELWIEGIVTVILLLMLNFALLVLINQMIAHNPGLENAIWGVKTNLTF
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCEE
GSRGFHLWSWSNLFLALMAIADVIVVYWRLARRYRQMQMRHVIAELHFIADGHLDHRIKF
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
EVNTELQKVVSSINALVDSTVNSMAEERRIEQSKDELITNVSHDIRTPLTSIIGYLGLIE
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHQYRSEEELLKYTHTAYLKAKQMKVLVEDLFEYTKVKSTTTKLNPVRFNMIAMLEQLAA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SFELEAHKKHMRILVSGEPDPLMMEADTEKLGRVFNNLIVNALKYGQGGRHIFLDAQRIG
HHHHHHHHCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCC
SEVVVKVSNDGAQIPNDALSQLFDRFYRVEESRSQETGGTGLGLAIAQSIIALHGGYIYA
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEE
DSSPEITSFVIHLPLKVGASLTDNKNKSNEEAGKSK
CCCCCHHHHHEEEHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]