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The map label for this gene is dacA [H]

Identifier: 116493792

GI number: 116493792

Start: 215872

End: 217182

Strand: Direct

Name: dacA [H]

Synonym: LSEI_0221

Alternate gene names: 116493792

Gene position: 215872-217182 (Clockwise)

Preceding gene: 116493791

Following gene: 116493793

Centisome position: 7.46

GC content: 45.61

Gene sequence:

>1311_bases
ATGAAAAAGTTGATGCGCGGGTTGATTGCCGCAGTGGTGGCGGTATTAATGATTGGGGGTGCTGCGCCAGCAATGTCGGT
ATCTGCGGCAGAGAGCAGCGATACCAATCTAGTTGACGCCAAGGCAGCAGTAGCCATTGAACCGACTACTGGCAAGATAT
TATATGCTAAGAATGCCAGCAGTGTTTTACCAATTGCATCTATGACCAAAATGTTGAGCATTGAGCTGGTGCTTGACGCA
ATTAAGTCCGGTAAGATCAATTGGGATGACAAAGTGACACTGAATAACGCAATCTGGGAACTCAGTCAGAATCGTGATCT
TAGTAATGTACCGTTGCGGCGCGATGGCTCTTATTCAGTCCGCGAACTGTACGATGCTTCTTTAATTTATTCGGCGAATG
CAGCGATGATGTTATTAGCTGAAAAAGTAGCGGGCAGTCAGGAGAAATTTGTCAGTCAGATGACTGCAAAACTCAATAGC
TGGGGTATCACCGATGCAAAAATTGTCAACGTGACCGGTTTGAATAACAGCCAATTGAAAGATGCTAAGGTACCCGGTAC
AGATGATAACGCGGAGAACGAAATGTCCGCAAAAGATATGGCCCAGGTGGCACAACATTTACTGAAGGATCATCCAGAAG
TCCTTAAGACCACGAGTATTGCTAAACAGACATTCCGTAAGGGAACCAGTGATGCGATCGATATGGAAAATTGGAACTGG
ATGCTCAAGGGCCTGGTGTCTTATGATAAGAACCTCCCAGTTGATGGCTTGAAGACAGGTACTTCTGATGCCGCTGGGGA
TTGTTTTACAGGGACGGTTGAGAAAAATGGCATGCGAATTATTACGGTTATCTTGCATGCAAATGGTGATGCTAAAACTC
GCCGTTTTGATCAAACCAAAAAATTGATGACCAATGTTTTGGAAAATTGGAAAACGATGACGTTAGCTGAAGCTGGTCAG
GGGATTAAAGGGTACGACAGCGTCAAGGTCAATCGTGGTCAATCACGGACAACGCCGATTGCGCCGTCAACCAAGGCTGT
TGCTGTTGTGCCATCTAACAGTAGCAAACAATCACTTCAATATACTTACACGCCAGCAAAAGGCGTGAAGAAGAATACTG
TCGAAGCACCAGTCAAAAAAGGTACCACGATTGCGCAGCTGGAAGTTAGCGCCAAAGGTGACAACCTTGGCTTTGTCGGC
AGTTCAGCAAGCGAAGGCATTACCTTAAAGACCACCAAGACCGATAACAAAGCAAACTTCTTTGTCCTGATCTTCCGTGG
CATTGGCGATTTCTTCAGCAATATTTTCTAG

Upstream 100 bases:

>100_bases
GCCCGGAGATAACAAGCTTTGTGATTCATTTACCATTAAAAGTTGGCGCTTCGCTGACAGACAATAAAAATAAATCTAAT
GAGGAAGCAGGAAAGTCAAA

Downstream 100 bases:

>100_bases
TCCAGCAGTTACCTCATTGATCCAAAAACGAAAAAGTGGTGAATGTCCTGAACTCCGAATGGAGATCATCACGTTCACCT
TTTTTGCAACAAGTAGAGAG

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MKKLMRGLIAAVVAVLMIGGAAPAMSVSAAESSDTNLVDAKAAVAIEPTTGKILYAKNASSVLPIASMTKMLSIELVLDA
IKSGKINWDDKVTLNNAIWELSQNRDLSNVPLRRDGSYSVRELYDASLIYSANAAMMLLAEKVAGSQEKFVSQMTAKLNS
WGITDAKIVNVTGLNNSQLKDAKVPGTDDNAENEMSAKDMAQVAQHLLKDHPEVLKTTSIAKQTFRKGTSDAIDMENWNW
MLKGLVSYDKNLPVDGLKTGTSDAAGDCFTGTVEKNGMRIITVILHANGDAKTRRFDQTKKLMTNVLENWKTMTLAEAGQ
GIKGYDSVKVNRGQSRTTPIAPSTKAVAVVPSNSSKQSLQYTYTPAKGVKKNTVEAPVKKGTTIAQLEVSAKGDNLGFVG
SSASEGITLKTTKTDNKANFFVLIFRGIGDFFSNIF

Sequences:

>Translated_436_residues
MKKLMRGLIAAVVAVLMIGGAAPAMSVSAAESSDTNLVDAKAAVAIEPTTGKILYAKNASSVLPIASMTKMLSIELVLDA
IKSGKINWDDKVTLNNAIWELSQNRDLSNVPLRRDGSYSVRELYDASLIYSANAAMMLLAEKVAGSQEKFVSQMTAKLNS
WGITDAKIVNVTGLNNSQLKDAKVPGTDDNAENEMSAKDMAQVAQHLLKDHPEVLKTTSIAKQTFRKGTSDAIDMENWNW
MLKGLVSYDKNLPVDGLKTGTSDAAGDCFTGTVEKNGMRIITVILHANGDAKTRRFDQTKKLMTNVLENWKTMTLAEAGQ
GIKGYDSVKVNRGQSRTTPIAPSTKAVAVVPSNSSKQSLQYTYTPAKGVKKNTVEAPVKKGTTIAQLEVSAKGDNLGFVG
SSASEGITLKTTKTDNKANFFVLIFRGIGDFFSNIF
>Mature_436_residues
MKKLMRGLIAAVVAVLMIGGAAPAMSVSAAESSDTNLVDAKAAVAIEPTTGKILYAKNASSVLPIASMTKMLSIELVLDA
IKSGKINWDDKVTLNNAIWELSQNRDLSNVPLRRDGSYSVRELYDASLIYSANAAMMLLAEKVAGSQEKFVSQMTAKLNS
WGITDAKIVNVTGLNNSQLKDAKVPGTDDNAENEMSAKDMAQVAQHLLKDHPEVLKTTSIAKQTFRKGTSDAIDMENWNW
MLKGLVSYDKNLPVDGLKTGTSDAAGDCFTGTVEKNGMRIITVILHANGDAKTRRFDQTKKLMTNVLENWKTMTLAEAGQ
GIKGYDSVKVNRGQSRTTPIAPSTKAVAVVPSNSSKQSLQYTYTPAKGVKKNTVEAPVKKGTTIAQLEVSAKGDNLGFVG
SSASEGITLKTTKTDNKANFFVLIFRGIGDFFSNIF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=400, Percent_Identity=28.5, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1786851, Length=378, Percent_Identity=27.5132275132275, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI87082022, Length=324, Percent_Identity=27.4691358024691, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87082054, Length=281, Percent_Identity=25.2669039145907, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 46902; Mature: 46902

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLMRGLIAAVVAVLMIGGAAPAMSVSAAESSDTNLVDAKAAVAIEPTTGKILYAKNAS
CHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCEEECCEEEEEECCCCEEEEECCCC
SVLPIASMTKMLSIELVLDAIKSGKINWDDKVTLNNAIWELSQNRDLSNVPLRRDGSYSV
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHCCCCCCCCCCCCCCCCHH
RELYDASLIYSANAAMMLLAEKVAGSQEKFVSQMTAKLNSWGITDAKIVNVTGLNNSQLK
HHHHCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
DAKVPGTDDNAENEMSAKDMAQVAQHLLKDHPEVLKTTSIAKQTFRKGTSDAIDMENWNW
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
MLKGLVSYDKNLPVDGLKTGTSDAAGDCFTGTVEKNGMRIITVILHANGDAKTRRFDQTK
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEECEECCCCCEEEEEEEECCCCHHHHHHHHHH
KLMTNVLENWKTMTLAEAGQGIKGYDSVKVNRGQSRTTPIAPSTKAVAVVPSNSSKQSLQ
HHHHHHHHHHHEEEHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEE
YTYTPAKGVKKNTVEAPVKKGTTIAQLEVSAKGDNLGFVGSSASEGITLKTTKTDNKANF
EEECCCCCCCCCCCCCHHHCCCEEEEEEEECCCCCEEEECCCCCCCEEEEEECCCCCCCE
FVLIFRGIGDFFSNIF
EEEEEHHHHHHHHHCC
>Mature Secondary Structure
MKKLMRGLIAAVVAVLMIGGAAPAMSVSAAESSDTNLVDAKAAVAIEPTTGKILYAKNAS
CHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCEEECCEEEEEECCCCEEEEECCCC
SVLPIASMTKMLSIELVLDAIKSGKINWDDKVTLNNAIWELSQNRDLSNVPLRRDGSYSV
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECHHHHHHHCCCCCCCCCCCCCCCCHH
RELYDASLIYSANAAMMLLAEKVAGSQEKFVSQMTAKLNSWGITDAKIVNVTGLNNSQLK
HHHHCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
DAKVPGTDDNAENEMSAKDMAQVAQHLLKDHPEVLKTTSIAKQTFRKGTSDAIDMENWNW
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
MLKGLVSYDKNLPVDGLKTGTSDAAGDCFTGTVEKNGMRIITVILHANGDAKTRRFDQTK
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEECEECCCCCEEEEEEEECCCCHHHHHHHHHH
KLMTNVLENWKTMTLAEAGQGIKGYDSVKVNRGQSRTTPIAPSTKAVAVVPSNSSKQSLQ
HHHHHHHHHHHEEEHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEE
YTYTPAKGVKKNTVEAPVKKGTTIAQLEVSAKGDNLGFVGSSASEGITLKTTKTDNKANF
EEECCCCCCCCCCCCCHHHCCCEEEEEEEECCCCCEEEECCCCCCCEEEEEECCCCCCCE
FVLIFRGIGDFFSNIF
EEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 6768745; 3087956; 6780559; 111240 [H]