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The map label for this gene is gyrA [H]

Identifier: 116493580

GI number: 116493580

Start: 6920

End: 9541

Strand: Direct

Name: gyrA [H]

Synonym: LSEI_0006

Alternate gene names: 116493580

Gene position: 6920-9541 (Clockwise)

Preceding gene: 116493579

Following gene: 116493582

Centisome position: 0.24

GC content: 48.63

Gene sequence:

>2622_bases
ATGGATGATCGTCAAGAAAGCCGAATTACCAATGTGAACCTCGGCGAAACAATGCGTAAATCATTCCTTGAATACGCGAT
GAGTGTCATTGTGGCGCGGGCCTTGCCTGATGTGCGCGATGGCCTCAAGCCGGTTCAACGGCGGATTCTGTACGGTATGA
ATGAACTCGGCGTGACGCCGGATAAACCGTACAAGAAGAGTGCGCGTATTGTTGGGGATGTCATGGGGAAATACCATCCG
CATGGTGATAGCTCGATTTATGAAGGGCTCGTACGAATGGCACAGGACTTCAGCTATCGTTACATGCTGGTCGATGGCCA
CGGGAACTTTGGGTCTGTCGATGGTGACGGTGCCGCTGCGATGCGGTATACCGAAGCTCGCATGAGCAAAATAGCAGTCG
AGATGCTGCGTGACATCAACAAAGATACGATTGATTTTCAGGATAACTATGATGGCACGGAAAAAGAACCGGTTGTTTTA
CCGGCACGTTTTCCAAATCTGTTGGTCAATGGGGCGACTGGGATTGCGGTCGGTATGACGACCAATATTCCGCCGCATAA
TCTGTCAGAGACTATCTCGGCGCTGCACGTGTTGATGGATAATCCAGATGCCACGACAGCTGACCTAATGCAGGCACTGC
CAGGACCTGATTTTCCAACCGGCGGCGTTGTGATGGGTAAGTCAGGCATTCGCCATGCTTATGAAACTGGCCGCGGCACG
ATTGTTTTGCGCGGGAAAGTTGATGTTCAGACGGAGAAGTCTGGCCGTGAACGGATCGTGATTACCGAGATTCCATATAT
GGTTAACAAAGCCAAAATGGTTGAACGGATCGCAGATTTGGTTCATGAAAAGAAAATCGATGGGATCGTAACCTTGCGGG
ATGAATCTGACCGTGACGGGATGCGGATCGTCATTGATGTGCGCCGTGATGCTAGTGCATCAGTTATTTTGAATAACTTG
TACAAGCTCACGCCACTACAAACCGGCTTTTCATTCAACATGGTGGCAATCGTTAATGGTGCGCCAAAAGTGTTGAGCTT
GAAACAGATCCTGCAATATTATCTGGATCATCAAGAAAACGTCATTCGTCGGCGGACGCAATTTGACCTGCGTAAGGCCA
ACGCTCGTGAACATATCCTCGAAGGATTACGGATTGCGCTTGATCATATTGATGAAATCATCACGATTATCCGTAGTTCT
GAAACTGGCGACAAGGCAAAAGTCATCTTGATGGACAAGTTTAAGTTGTCTGACAAGCAGAGCCAAGCAATCTTGGATAT
GCGGCTGGTTCGGTTGACCGGTTTGGAACGCGAGAAGGTTGAAAGCGAATATAAGGATGTTGAAGCAGCGATTGCTGATT
ATACTGATATTCTTGCTAGACCAGAACGTGTTCATCAGATCATCTACAACGAATTGCTGGATATTCAGAAGAAGTTCGGC
GATAAGCGCCGTACTGAATTACTGGTCGGTGAAGTGCTCAGTCTTGAAGATGAAGATCTGATCGAGCAAGAAGACGTGGT
CATCACGTTGAGTCATAACGGCTACGTCAAGCGATTGGCAACCAGTGAATTCAAGGCACAAAATCGCGGGGGTCGCGGTA
TTCAGGGGATGAACGTCCATGATGACGATTTCGTTGAACGCCTGATTTCGACTTCCACGCATGATGTCCTGCTCTTCTTC
ACCAACAAAGGTAAAGTTTATCGCAGCAAGGGTTACGAAATTCCTGAGTACGGTCGAACCGCAAAAGGGATTCCAATTAT
CAACTTGCTTGGTGTCGGTGCCGGTGAAAAGATTCAAACGGTTATCAACGTGCATGAAGGCGAAAATGATGATCGTTATC
TCTTCTTCGTCACGCAAAAAGGCGTTGTTAAGCGCACACCGGTGAAGGAATTCGCCAATATTCGCAGCAACGGACTGATT
GCGTTGAATCTCAAGGATGAAGACGAACTGAATAATGTCATCCTAACGTCCGGACAAGACAATATCCTGATCGGGACGCA
TCTGGGTTACAGTGTGACCTTCAAGGAACAAGACGTTCGCTCAATGGGACGTTCAGCGACTGGGGTTCGAGGTATTCGTT
TACGTGAACACGATTACGTAGTGGGTTCCGACATTCTCAAACCCGATTCCGAAGTCTTTGTCATTTCAGAAAAAGGTTAC
GGTAAACGGACGGCAGCGAAGGAATATCCGATTAAAGGTCGTGGCGGTAAAGGGATCAAGACAGCCAACATCACCGCCAA
GAATGGTCCGTTGGCAGGGGTCACCACCGTTGATGGTACCGAAGATATTCTTGTTATGACTGATTCTGGTGTCATGATTC
GCTTCAATATTCAAAGTGTTTCGCAAACTGGCCGAGCAACGTTAGGGGTTCGTTTAATTCGAGTTGACGACGATGCCAAG
GTTGCAACGATGGCCAAGGTTGAACCGGAAAGCGACGATCCAGACGATGGCAGCAAGCCAGATCAGCCAACTAGCCCGAC
AGACGGTTCTGCTGCACCAACCTCGCAGCAGCCAGCTGATGGTTCGTATGCTGGCGATGCCGATCAGCAAGTGAGCCAAT
TGCTTGATCGCGCTGAAGCTGATCAACCAGAGCAACATGATACTGGCGACCAGCCTGAATAA

Upstream 100 bases:

>100_bases
AACGCTGTGTTTGTTGATCCGAACAACATTGATGCTTGATTGATCAGTTTAACTAAGTCAGCTTTTGCATGAGAATTTTT
GAGAGGAGACTTTTCGCTTA

Downstream 100 bases:

>100_bases
TCTAATAATGCTAGCATCAATTGAGCTGATTCACGCTCAATAAAAATACCGGTCGAAATGGCAATTTGCCACTTCGACCG
GTATTTGTTTGCGTACAGAT

Product: DNA gyrase, A subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 873; Mature: 873

Protein sequence:

>873_residues
MDDRQESRITNVNLGETMRKSFLEYAMSVIVARALPDVRDGLKPVQRRILYGMNELGVTPDKPYKKSARIVGDVMGKYHP
HGDSSIYEGLVRMAQDFSYRYMLVDGHGNFGSVDGDGAAAMRYTEARMSKIAVEMLRDINKDTIDFQDNYDGTEKEPVVL
PARFPNLLVNGATGIAVGMTTNIPPHNLSETISALHVLMDNPDATTADLMQALPGPDFPTGGVVMGKSGIRHAYETGRGT
IVLRGKVDVQTEKSGRERIVITEIPYMVNKAKMVERIADLVHEKKIDGIVTLRDESDRDGMRIVIDVRRDASASVILNNL
YKLTPLQTGFSFNMVAIVNGAPKVLSLKQILQYYLDHQENVIRRRTQFDLRKANAREHILEGLRIALDHIDEIITIIRSS
ETGDKAKVILMDKFKLSDKQSQAILDMRLVRLTGLEREKVESEYKDVEAAIADYTDILARPERVHQIIYNELLDIQKKFG
DKRRTELLVGEVLSLEDEDLIEQEDVVITLSHNGYVKRLATSEFKAQNRGGRGIQGMNVHDDDFVERLISTSTHDVLLFF
TNKGKVYRSKGYEIPEYGRTAKGIPIINLLGVGAGEKIQTVINVHEGENDDRYLFFVTQKGVVKRTPVKEFANIRSNGLI
ALNLKDEDELNNVILTSGQDNILIGTHLGYSVTFKEQDVRSMGRSATGVRGIRLREHDYVVGSDILKPDSEVFVISEKGY
GKRTAAKEYPIKGRGGKGIKTANITAKNGPLAGVTTVDGTEDILVMTDSGVMIRFNIQSVSQTGRATLGVRLIRVDDDAK
VATMAKVEPESDDPDDGSKPDQPTSPTDGSAAPTSQQPADGSYAGDADQQVSQLLDRAEADQPEQHDTGDQPE

Sequences:

>Translated_873_residues
MDDRQESRITNVNLGETMRKSFLEYAMSVIVARALPDVRDGLKPVQRRILYGMNELGVTPDKPYKKSARIVGDVMGKYHP
HGDSSIYEGLVRMAQDFSYRYMLVDGHGNFGSVDGDGAAAMRYTEARMSKIAVEMLRDINKDTIDFQDNYDGTEKEPVVL
PARFPNLLVNGATGIAVGMTTNIPPHNLSETISALHVLMDNPDATTADLMQALPGPDFPTGGVVMGKSGIRHAYETGRGT
IVLRGKVDVQTEKSGRERIVITEIPYMVNKAKMVERIADLVHEKKIDGIVTLRDESDRDGMRIVIDVRRDASASVILNNL
YKLTPLQTGFSFNMVAIVNGAPKVLSLKQILQYYLDHQENVIRRRTQFDLRKANAREHILEGLRIALDHIDEIITIIRSS
ETGDKAKVILMDKFKLSDKQSQAILDMRLVRLTGLEREKVESEYKDVEAAIADYTDILARPERVHQIIYNELLDIQKKFG
DKRRTELLVGEVLSLEDEDLIEQEDVVITLSHNGYVKRLATSEFKAQNRGGRGIQGMNVHDDDFVERLISTSTHDVLLFF
TNKGKVYRSKGYEIPEYGRTAKGIPIINLLGVGAGEKIQTVINVHEGENDDRYLFFVTQKGVVKRTPVKEFANIRSNGLI
ALNLKDEDELNNVILTSGQDNILIGTHLGYSVTFKEQDVRSMGRSATGVRGIRLREHDYVVGSDILKPDSEVFVISEKGY
GKRTAAKEYPIKGRGGKGIKTANITAKNGPLAGVTTVDGTEDILVMTDSGVMIRFNIQSVSQTGRATLGVRLIRVDDDAK
VATMAKVEPESDDPDDGSKPDQPTSPTDGSAAPTSQQPADGSYAGDADQQVSQLLDRAEADQPEQHDTGDQPE
>Mature_873_residues
MDDRQESRITNVNLGETMRKSFLEYAMSVIVARALPDVRDGLKPVQRRILYGMNELGVTPDKPYKKSARIVGDVMGKYHP
HGDSSIYEGLVRMAQDFSYRYMLVDGHGNFGSVDGDGAAAMRYTEARMSKIAVEMLRDINKDTIDFQDNYDGTEKEPVVL
PARFPNLLVNGATGIAVGMTTNIPPHNLSETISALHVLMDNPDATTADLMQALPGPDFPTGGVVMGKSGIRHAYETGRGT
IVLRGKVDVQTEKSGRERIVITEIPYMVNKAKMVERIADLVHEKKIDGIVTLRDESDRDGMRIVIDVRRDASASVILNNL
YKLTPLQTGFSFNMVAIVNGAPKVLSLKQILQYYLDHQENVIRRRTQFDLRKANAREHILEGLRIALDHIDEIITIIRSS
ETGDKAKVILMDKFKLSDKQSQAILDMRLVRLTGLEREKVESEYKDVEAAIADYTDILARPERVHQIIYNELLDIQKKFG
DKRRTELLVGEVLSLEDEDLIEQEDVVITLSHNGYVKRLATSEFKAQNRGGRGIQGMNVHDDDFVERLISTSTHDVLLFF
TNKGKVYRSKGYEIPEYGRTAKGIPIINLLGVGAGEKIQTVINVHEGENDDRYLFFVTQKGVVKRTPVKEFANIRSNGLI
ALNLKDEDELNNVILTSGQDNILIGTHLGYSVTFKEQDVRSMGRSATGVRGIRLREHDYVVGSDILKPDSEVFVISEKGY
GKRTAAKEYPIKGRGGKGIKTANITAKNGPLAGVTTVDGTEDILVMTDSGVMIRFNIQSVSQTGRATLGVRLIRVDDDAK
VATMAKVEPESDDPDDGSKPDQPTSPTDGSAAPTSQQPADGSYAGDADQQVSQLLDRAEADQPEQHDTGDQPE

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=325, Percent_Identity=25.5384615384615, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI19913408, Length=339, Percent_Identity=25.0737463126844, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI1788562, Length=859, Percent_Identity=48.4284051222352, Blast_Score=811, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=678, Percent_Identity=35.693215339233, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17535065, Length=174, Percent_Identity=31.0344827586207, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI212645845, Length=198, Percent_Identity=30.3030303030303, Blast_Score=70, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=356, Percent_Identity=26.123595505618, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17136538, Length=174, Percent_Identity=31.6091954022989, Blast_Score=77, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 96773; Mature: 96773

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDRQESRITNVNLGETMRKSFLEYAMSVIVARALPDVRDGLKPVQRRILYGMNELGVTP
CCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHCCCCC
DKPYKKSARIVGDVMGKYHPHGDSSIYEGLVRMAQDFSYRYMLVDGHGNFGSVDGDGAAA
CCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHH
MRYTEARMSKIAVEMLRDINKDTIDFQDNYDGTEKEPVVLPARFPNLLVNGATGIAVGMT
HHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEECCCCCEEEECCCEEEEEEE
TNIPPHNLSETISALHVLMDNPDATTADLMQALPGPDFPTGGVVMGKSGIRHAYETGRGT
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEECCCCCHHHHHCCCEE
IVLRGKVDVQTEKSGRERIVITEIPYMVNKAKMVERIADLVHEKKIDGIVTLRDESDRDG
EEEEEEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
MRIVIDVRRDASASVILNNLYKLTPLQTGFSFNMVAIVNGAPKVLSLKQILQYYLDHQEN
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCHHH
VIRRRTQFDLRKANAREHILEGLRIALDHIDEIITIIRSSETGDKAKVILMDKFKLSDKQ
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCH
SQAILDMRLVRLTGLEREKVESEYKDVEAAIADYTDILARPERVHQIIYNELLDIQKKFG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
DKRRTELLVGEVLSLEDEDLIEQEDVVITLSHNGYVKRLATSEFKAQNRGGRGIQGMNVH
CHHHHHHHHHHHHCCCCHHCCCCCCEEEEEECCCEEEHHHHHHHHHCCCCCCCCCCCCCC
DDDFVERLISTSTHDVLLFFTNKGKVYRSKGYEIPEYGRTAKGIPIINLLGVGAGEKIQT
CHHHHHHHHCCCCCEEEEEEECCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCEEE
VINVHEGENDDRYLFFVTQKGVVKRTPVKEFANIRSNGLIALNLKDEDELNNVILTSGQD
EEEEECCCCCCEEEEEEECCCCEECCCHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCC
NILIGTHLGYSVTFKEQDVRSMGRSATGVRGIRLREHDYVVGSDILKPDSEVFVISEKGY
CEEEEECCCEEEEECHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCCCCEEEEECCCC
GKRTAAKEYPIKGRGGKGIKTANITAKNGPLAGVTTVDGTEDILVMTDSGVMIRFNIQSV
CCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCEEEEECCCEEEEEEEHHH
SQTGRATLGVRLIRVDDDAKVATMAKVEPESDDPDDGSKPDQPTSPTDGSAAPTSQQPAD
HCCCCEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GSYAGDADQQVSQLLDRAEADQPEQHDTGDQPE
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDDRQESRITNVNLGETMRKSFLEYAMSVIVARALPDVRDGLKPVQRRILYGMNELGVTP
CCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHCCCCC
DKPYKKSARIVGDVMGKYHPHGDSSIYEGLVRMAQDFSYRYMLVDGHGNFGSVDGDGAAA
CCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHH
MRYTEARMSKIAVEMLRDINKDTIDFQDNYDGTEKEPVVLPARFPNLLVNGATGIAVGMT
HHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEECCCCCEEEECCCEEEEEEE
TNIPPHNLSETISALHVLMDNPDATTADLMQALPGPDFPTGGVVMGKSGIRHAYETGRGT
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEECCCCCHHHHHCCCEE
IVLRGKVDVQTEKSGRERIVITEIPYMVNKAKMVERIADLVHEKKIDGIVTLRDESDRDG
EEEEEEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
MRIVIDVRRDASASVILNNLYKLTPLQTGFSFNMVAIVNGAPKVLSLKQILQYYLDHQEN
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCHHH
VIRRRTQFDLRKANAREHILEGLRIALDHIDEIITIIRSSETGDKAKVILMDKFKLSDKQ
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCH
SQAILDMRLVRLTGLEREKVESEYKDVEAAIADYTDILARPERVHQIIYNELLDIQKKFG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
DKRRTELLVGEVLSLEDEDLIEQEDVVITLSHNGYVKRLATSEFKAQNRGGRGIQGMNVH
CHHHHHHHHHHHHCCCCHHCCCCCCEEEEEECCCEEEHHHHHHHHHCCCCCCCCCCCCCC
DDDFVERLISTSTHDVLLFFTNKGKVYRSKGYEIPEYGRTAKGIPIINLLGVGAGEKIQT
CHHHHHHHHCCCCCEEEEEEECCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCEEE
VINVHEGENDDRYLFFVTQKGVVKRTPVKEFANIRSNGLIALNLKDEDELNNVILTSGQD
EEEEECCCCCCEEEEEEECCCCEECCCHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCC
NILIGTHLGYSVTFKEQDVRSMGRSATGVRGIRLREHDYVVGSDILKPDSEVFVISEKGY
CEEEEECCCEEEEECHHHHHHHCCCCCCCCEEEEECCCEEECCCCCCCCCCEEEEECCCC
GKRTAAKEYPIKGRGGKGIKTANITAKNGPLAGVTTVDGTEDILVMTDSGVMIRFNIQSV
CCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCEEEEECCCEEEEEEEHHH
SQTGRATLGVRLIRVDDDAKVATMAKVEPESDDPDDGSKPDQPTSPTDGSAAPTSQQPAD
HCCCCEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GSYAGDADQQVSQLLDRAEADQPEQHDTGDQPE
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]