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The map label for this gene is gyrB [H]

Identifier: 116493579

GI number: 116493579

Start: 4897

End: 6858

Strand: Direct

Name: gyrB [H]

Synonym: LSEI_0005

Alternate gene names: 116493579

Gene position: 4897-6858 (Clockwise)

Preceding gene: 116493578

Following gene: 116493580

Centisome position: 0.17

GC content: 49.44

Gene sequence:

>1962_bases
GTGACGGACAAGAAAGAAACGGCCGAAGAGAAGAAGGATGAACTGGCGGCTGAGTATGATGCCAGCCAGATTCAAGTACT
TTCAGGACTTGAAGCGGTTCGGAAGCGTCCAGGGATGTATATTGGCTCGACAAGCAGCCAAGGCTTGCATCATTTGGTCT
GGGAAATCATTGATAACGGGATTGATGAAGCGCTGGCTGGATTCGCAACACGGATTGAAGTTGAAGTTAACCCAGATAAT
AGTGTGACGGTAACAGATGACGGTCGCGGGATTCCAGTCGATATCCAGTCTAAAACCGGACGGCCAGCACTTGAAACGGT
TTATACCGTTTTACATGCCGGTGGTAAATTCGGCGGCGGCGGCTACAAAGTTTCCGGTGGCCTACATGGTGTCGGGGCAT
CAGTCGTGAATGCGTTGTCGACTGATTTGGACGTCACGGTTACTCGTGGTGGCAAACGTTACTACATTGACTTTATTCGC
GGCAAGGTGAACATGCCGATGAAAGTGATTGGCACGGCACCGGAGCACGAACATGGCACCAAGGTTCATTTTCTGCCTGA
TCCGGACATTTTTACCGAAATTAGAACATTCGATGATAAGATTCTGACCACCAGAATTCGCGAACTGGCTTTCTTGAACA
AGGGTCTCAAGTTGACCTTTACCGACAAGCGCGCGGCAACGCACGAGAAGCTGGTATTCCATTATGAAGGCGGCCTCAAG
TCTTACGTTGACTTTTTAACTGAGAAAAAAGAAAACCTGTTACAAGAACCGATTTATGTGGAAGGACAAGACAAAGGCAT
CACGGTGGAAGTCGCGTTGCAATACACCAACGACTATCACAGCACCTTGTTGACGTTTGCCAACAACATCCATACGTATG
AAGGCGGCACGCATGAGACCGGGTTTAAAACGGCGCTGACACGGGTCATTAATGATTACGCTCGCCGGTCTGGTGCGCTA
AAAGACAGCGATGATCCGTTAAGCGGGGATGATGTTCGTGAGGGCTTGACGGCGGTTGTTTCCGTGAAGCATCCAGATCC
GCAGTTTGAAGGGCAAACTAAAACCAAATTAGGGAACTCGGATGCCCGAACGGTGGTTGACCGTACCTTCTCTGACCACT
TCAATAAGTTTCTCATGGAACACCCAGCTGATGGCAAGCTAATCATTGACAAAGCCATGCTGGCCAGCAAGGCACGTTTG
GCGGCTAAGCGGGCGCGTGAAGTGACCCGGAAAAAGAGCGGGTTGGAAATTTCGAACCTGCCTGGTAAATTGGCCGACAA
CACCAGTAAGGACCCAGAGATCTCTGAATTATTTATCGTCGAAGGGGATTCCGCTGGTGGTTCTGCCAAATCAGGGCGTA
GTCGCCTGACGCAGGCCATTTTGCCAATTCGTGGGAAAATTCTGAATGTCGAAAAGGCTTCAATGGAACGCATCTTGGCC
AATGAAGAAATTCGAACCTTATTCACCGCGATGGGTACCGGTTTTGGGCAGGACTTTGATATCAATAAGGCTCGATATCA
CAAACTGATCATTATGACCGATGCCGATGTTGATGGTGCCCACATTCGGACTTTGTTGCTGACGTTGATTTATCGCTACA
TGCGGCCAGTGCTTGACGCTGGCTATGTCTACATCGCACAGCCGCCGTTGTATCGGTTACGCCAAGGCAAAATGACGCAA
TATATCGATTCCGATGAAGAATTGCAAGACATTCTAGGCCAGTTGCCGCCGAGTCCAAAGCCAGAGATTCAGCGTTACAA
AGGGCTTGGGGAAATGGACGCCAACCAGCTATGGGAGACGACCATGGATCCGGATAATCGGCGCTTACTGCGGGTATCGC
CAAAGGATGCCGAAGCTGCAGATGGCGTTTTTGAAATGTTGATGGGTGATCATGTTGAACCGCGGCGTAAATTTATTGAA
GATAACGCTGTGTTTGTTGATCCGAACAACATTGATGCTTGA

Upstream 100 bases:

>100_bases
GTGGGCTTGAACGTGATGGCCGGTTTTTGGCCATTGCGTTCAAGGTCCTTATGTGCAGGTTTCTGTGCCAGTGAACGCAT
TTTAACAAGGAGGATAACCA

Downstream 100 bases:

>100_bases
TTGATCAGTTTAACTAAGTCAGCTTTTGCATGAGAATTTTTGAGAGGAGACTTTTCGCTTAATGGATGATCGTCAAGAAA
GCCGAATTACCAATGTGAAC

Product: DNA gyrase, B subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 653; Mature: 652

Protein sequence:

>653_residues
MTDKKETAEEKKDELAAEYDASQIQVLSGLEAVRKRPGMYIGSTSSQGLHHLVWEIIDNGIDEALAGFATRIEVEVNPDN
SVTVTDDGRGIPVDIQSKTGRPALETVYTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTDLDVTVTRGGKRYYIDFIR
GKVNMPMKVIGTAPEHEHGTKVHFLPDPDIFTEIRTFDDKILTTRIRELAFLNKGLKLTFTDKRAATHEKLVFHYEGGLK
SYVDFLTEKKENLLQEPIYVEGQDKGITVEVALQYTNDYHSTLLTFANNIHTYEGGTHETGFKTALTRVINDYARRSGAL
KDSDDPLSGDDVREGLTAVVSVKHPDPQFEGQTKTKLGNSDARTVVDRTFSDHFNKFLMEHPADGKLIIDKAMLASKARL
AAKRAREVTRKKSGLEISNLPGKLADNTSKDPEISELFIVEGDSAGGSAKSGRSRLTQAILPIRGKILNVEKASMERILA
NEEIRTLFTAMGTGFGQDFDINKARYHKLIIMTDADVDGAHIRTLLLTLIYRYMRPVLDAGYVYIAQPPLYRLRQGKMTQ
YIDSDEELQDILGQLPPSPKPEIQRYKGLGEMDANQLWETTMDPDNRRLLRVSPKDAEAADGVFEMLMGDHVEPRRKFIE
DNAVFVDPNNIDA

Sequences:

>Translated_653_residues
MTDKKETAEEKKDELAAEYDASQIQVLSGLEAVRKRPGMYIGSTSSQGLHHLVWEIIDNGIDEALAGFATRIEVEVNPDN
SVTVTDDGRGIPVDIQSKTGRPALETVYTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTDLDVTVTRGGKRYYIDFIR
GKVNMPMKVIGTAPEHEHGTKVHFLPDPDIFTEIRTFDDKILTTRIRELAFLNKGLKLTFTDKRAATHEKLVFHYEGGLK
SYVDFLTEKKENLLQEPIYVEGQDKGITVEVALQYTNDYHSTLLTFANNIHTYEGGTHETGFKTALTRVINDYARRSGAL
KDSDDPLSGDDVREGLTAVVSVKHPDPQFEGQTKTKLGNSDARTVVDRTFSDHFNKFLMEHPADGKLIIDKAMLASKARL
AAKRAREVTRKKSGLEISNLPGKLADNTSKDPEISELFIVEGDSAGGSAKSGRSRLTQAILPIRGKILNVEKASMERILA
NEEIRTLFTAMGTGFGQDFDINKARYHKLIIMTDADVDGAHIRTLLLTLIYRYMRPVLDAGYVYIAQPPLYRLRQGKMTQ
YIDSDEELQDILGQLPPSPKPEIQRYKGLGEMDANQLWETTMDPDNRRLLRVSPKDAEAADGVFEMLMGDHVEPRRKFIE
DNAVFVDPNNIDA
>Mature_652_residues
TDKKETAEEKKDELAAEYDASQIQVLSGLEAVRKRPGMYIGSTSSQGLHHLVWEIIDNGIDEALAGFATRIEVEVNPDNS
VTVTDDGRGIPVDIQSKTGRPALETVYTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTDLDVTVTRGGKRYYIDFIRG
KVNMPMKVIGTAPEHEHGTKVHFLPDPDIFTEIRTFDDKILTTRIRELAFLNKGLKLTFTDKRAATHEKLVFHYEGGLKS
YVDFLTEKKENLLQEPIYVEGQDKGITVEVALQYTNDYHSTLLTFANNIHTYEGGTHETGFKTALTRVINDYARRSGALK
DSDDPLSGDDVREGLTAVVSVKHPDPQFEGQTKTKLGNSDARTVVDRTFSDHFNKFLMEHPADGKLIIDKAMLASKARLA
AKRAREVTRKKSGLEISNLPGKLADNTSKDPEISELFIVEGDSAGGSAKSGRSRLTQAILPIRGKILNVEKASMERILAN
EEIRTLFTAMGTGFGQDFDINKARYHKLIIMTDADVDGAHIRTLLLTLIYRYMRPVLDAGYVYIAQPPLYRLRQGKMTQY
IDSDEELQDILGQLPPSPKPEIQRYKGLGEMDANQLWETTMDPDNRRLLRVSPKDAEAADGVFEMLMGDHVEPRRKFIED
NAVFVDPNNIDA

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=702, Percent_Identity=25.6410256410256, Blast_Score=147, Evalue=4e-35,
Organism=Homo sapiens, GI19913406, Length=672, Percent_Identity=24.8511904761905, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI48994957, Length=556, Percent_Identity=50.3597122302158, Blast_Score=543, Evalue=1e-155,
Organism=Escherichia coli, GI1789408, Length=630, Percent_Identity=40.3174603174603, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17535065, Length=663, Percent_Identity=24.73604826546, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=549, Percent_Identity=24.408014571949, Blast_Score=116, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324241, Length=595, Percent_Identity=25.546218487395, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17136538, Length=656, Percent_Identity=24.6951219512195, Blast_Score=130, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72487; Mature: 72356

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKKETAEEKKDELAAEYDASQIQVLSGLEAVRKRPGMYIGSTSSQGLHHLVWEIIDNG
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCC
IDEALAGFATRIEVEVNPDNSVTVTDDGRGIPVDIQSKTGRPALETVYTVLHAGGKFGGG
HHHHHHCCEEEEEEEECCCCEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCC
GYKVSGGLHGVGASVVNALSTDLDVTVTRGGKRYYIDFIRGKVNMPMKVIGTAPEHEHGT
CEEECCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEEEECCCCCCEEEEECCCCCCCCC
KVHFLPDPDIFTEIRTFDDKILTTRIRELAFLNKGLKLTFTDKRAATHEKLVFHYEGGLK
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHCCCCEEEEEECCCHH
SYVDFLTEKKENLLQEPIYVEGQDKGITVEVALQYTNDYHSTLLTFANNIHTYEGGTHET
HHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEEEECCCHHHHHHHHHHCCEECCCCCCCC
GFKTALTRVINDYARRSGALKDSDDPLSGDDVREGLTAVVSVKHPDPQFEGQTKTKLGNS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCHHHCCCCC
DARTVVDRTFSDHFNKFLMEHPADGKLIIDKAMLASKARLAAKRAREVTRKKSGLEISNL
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEECCC
PGKLADNTSKDPEISELFIVEGDSAGGSAKSGRSRLTQAILPIRGKILNVEKASMERILA
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEECHHHHHHHHHH
NEEIRTLFTAMGTGFGQDFDINKARYHKLIIMTDADVDGAHIRTLLLTLIYRYMRPVLDA
HHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
GYVYIAQPPLYRLRQGKMTQYIDSDEELQDILGQLPPSPKPEIQRYKGLGEMDANQLWET
CEEEEECCCHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHC
TMDPDNRRLLRVSPKDAEAADGVFEMLMGDHVEPRRKFIEDNAVFVDPNNIDA
CCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCEEECCCCCCC
>Mature Secondary Structure 
TDKKETAEEKKDELAAEYDASQIQVLSGLEAVRKRPGMYIGSTSSQGLHHLVWEIIDNG
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCC
IDEALAGFATRIEVEVNPDNSVTVTDDGRGIPVDIQSKTGRPALETVYTVLHAGGKFGGG
HHHHHHCCEEEEEEEECCCCEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCC
GYKVSGGLHGVGASVVNALSTDLDVTVTRGGKRYYIDFIRGKVNMPMKVIGTAPEHEHGT
CEEECCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEEEECCCCCCEEEEECCCCCCCCC
KVHFLPDPDIFTEIRTFDDKILTTRIRELAFLNKGLKLTFTDKRAATHEKLVFHYEGGLK
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHCCCCEEEEEECCCHH
SYVDFLTEKKENLLQEPIYVEGQDKGITVEVALQYTNDYHSTLLTFANNIHTYEGGTHET
HHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEEEECCCHHHHHHHHHHCCEECCCCCCCC
GFKTALTRVINDYARRSGALKDSDDPLSGDDVREGLTAVVSVKHPDPQFEGQTKTKLGNS
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCHHHCCCCC
DARTVVDRTFSDHFNKFLMEHPADGKLIIDKAMLASKARLAAKRAREVTRKKSGLEISNL
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEECCC
PGKLADNTSKDPEISELFIVEGDSAGGSAKSGRSRLTQAILPIRGKILNVEKASMERILA
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEECHHHHHHHHHH
NEEIRTLFTAMGTGFGQDFDINKARYHKLIIMTDADVDGAHIRTLLLTLIYRYMRPVLDA
HHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
GYVYIAQPPLYRLRQGKMTQYIDSDEELQDILGQLPPSPKPEIQRYKGLGEMDANQLWET
CEEEEECCCHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHC
TMDPDNRRLLRVSPKDAEAADGVFEMLMGDHVEPRRKFIEDNAVFVDPNNIDA
CCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377 [H]