Click here to switch to the map view.

The map label for this gene is exoA [H]

Identifier: 116493341

GI number: 116493341

Start: 1590245

End: 1591000

Strand: Reverse

Name: exoA [H]

Synonym: PEPE_1613

Alternate gene names: 116493341

Gene position: 1591000-1590245 (Counterclockwise)

Preceding gene: 116493343

Following gene: 116493340

Centisome position: 86.83

GC content: 35.32

Gene sequence:

>756_bases
TTGAAGCTTATTTCATGGAATGTAAATGGATTGCGTGCAGCGGTTAAGCATGGATTTCTGGATGTTTTTAAGGAACTAGA
TGCAGATATATTTTGCATCCAGGAAACAAAGTTGCAAGAGGGGCAAATTGAATTAGAATTGCCTCACTACTATCAATATT
GGAATTATGCGGAGAAAAAAGGATATTCTGGTACGGCAATTTTTACTAAAAAAAAGCCGCTTGCTGTAAGATATGGTCTT
GGAATTGAAGAACATGACCAAGAAGGTCGTATAATTACTTTAGAATTTGAAAAATTTTACGTAATTACTTGTTATACACC
AAATTCACAGCCAAAATTAAAGAGACTAGAATATCGAATGGCTTGGGATGATGCATTTAGAGCATACATAGACCAGTTAA
ATCAGCATAAACCCGTTATTTTCTGTGGAGATTTAAACGTGGCGCACCAAGAGATTGATTTAAAGAATGACAAAACTAAT
CATAAAAATGCAGGATTTACAGACGAGGAACGCAATAAATTTACACAGTTATTAAATAGTGGTTTTACAGATACATACCG
ATACTTCTACCCCTCAAAAGAAGGAGTTTATTCTTGGTGGAGCTATCGATTTAATGCACGTGCTAATAATGCTGGATGGC
GGATTGACTACTTTGTTAGTTCAAAGGCGTTGGATCAAAAATTGACTGACGCCCAGATTCATACTGAAATTTTTGGTTCC
GATCATTGTCCAGTCGAATTAGATCTTGATATATAA

Upstream 100 bases:

>100_bases
TAGGAAAGCAAGCTGATGTATTGCGTACTATCAGTAATAAAATATGCGATAATAGAATGGGAATATTAGTAAATTAATTT
TTACACTGGAGGAAGTCATT

Downstream 100 bases:

>100_bases
GTCATGGGAGAGAAAAGATGAGTTTACCAAAAAAATTGGAAGAGATGCAGGTTGAAGAAGCTAGTAAATTAGCGCAAAAC
CTTAGAGAGATTTTGGATCG

Product: exonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKLISWNVNGLRAAVKHGFLDVFKELDADIFCIQETKLQEGQIELELPHYYQYWNYAEKKGYSGTAIFTKKKPLAVRYGL
GIEEHDQEGRIITLEFEKFYVITCYTPNSQPKLKRLEYRMAWDDAFRAYIDQLNQHKPVIFCGDLNVAHQEIDLKNDKTN
HKNAGFTDEERNKFTQLLNSGFTDTYRYFYPSKEGVYSWWSYRFNARANNAGWRIDYFVSSKALDQKLTDAQIHTEIFGS
DHCPVELDLDI

Sequences:

>Translated_251_residues
MKLISWNVNGLRAAVKHGFLDVFKELDADIFCIQETKLQEGQIELELPHYYQYWNYAEKKGYSGTAIFTKKKPLAVRYGL
GIEEHDQEGRIITLEFEKFYVITCYTPNSQPKLKRLEYRMAWDDAFRAYIDQLNQHKPVIFCGDLNVAHQEIDLKNDKTN
HKNAGFTDEERNKFTQLLNSGFTDTYRYFYPSKEGVYSWWSYRFNARANNAGWRIDYFVSSKALDQKLTDAQIHTEIFGS
DHCPVELDLDI
>Mature_251_residues
MKLISWNVNGLRAAVKHGFLDVFKELDADIFCIQETKLQEGQIELELPHYYQYWNYAEKKGYSGTAIFTKKKPLAVRYGL
GIEEHDQEGRIITLEFEKFYVITCYTPNSQPKLKRLEYRMAWDDAFRAYIDQLNQHKPVIFCGDLNVAHQEIDLKNDKTN
HKNAGFTDEERNKFTQLLNSGFTDTYRYFYPSKEGVYSWWSYRFNARANNAGWRIDYFVSSKALDQKLTDAQIHTEIFGS
DHCPVELDLDI

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=257, Percent_Identity=48.2490272373541, Blast_Score=265, Evalue=3e-71,
Organism=Homo sapiens, GI18375503, Length=257, Percent_Identity=48.2490272373541, Blast_Score=265, Evalue=3e-71,
Organism=Homo sapiens, GI18375501, Length=257, Percent_Identity=48.2490272373541, Blast_Score=265, Evalue=3e-71,
Organism=Homo sapiens, GI18375507, Length=319, Percent_Identity=28.8401253918495, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1788046, Length=263, Percent_Identity=29.277566539924, Blast_Score=113, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71989536, Length=255, Percent_Identity=43.1372549019608, Blast_Score=204, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI71989539, Length=145, Percent_Identity=37.9310344827586, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI221330655, Length=254, Percent_Identity=48.8188976377953, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI17136678, Length=250, Percent_Identity=49.2, Blast_Score=262, Evalue=2e-70,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29517; Mature: 29517

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISWNVNGLRAAVKHGFLDVFKELDADIFCIQETKLQEGQIELELPHYYQYWNYAEKK
CEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCHHHHHHCHHHHC
GYSGTAIFTKKKPLAVRYGLGIEEHDQEGRIITLEFEKFYVITCYTPNSQPKLKRLEYRM
CCCCEEEEECCCCEEEEECCCCCCCCCCCCEEEEEEEEEEEEEEECCCCCCHHHHHHHHH
AWDDAFRAYIDQLNQHKPVIFCGDLNVAHQEIDLKNDKTNHKNAGFTDEERNKFTQLLNS
HHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
GFTDTYRYFYPSKEGVYSWWSYRFNARANNAGWRIDYFVSSKALDQKLTDAQIHTEIFGS
CCCCCEEEECCCCCCCEEEEEEEEECCCCCCCEEEEEEECCHHHHHHHCCHHEEEEECCC
DHCPVELDLDI
CCCCEEEEECC
>Mature Secondary Structure
MKLISWNVNGLRAAVKHGFLDVFKELDADIFCIQETKLQEGQIELELPHYYQYWNYAEKK
CEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCHHHHHHCHHHHC
GYSGTAIFTKKKPLAVRYGLGIEEHDQEGRIITLEFEKFYVITCYTPNSQPKLKRLEYRM
CCCCEEEEECCCCEEEEECCCCCCCCCCCCEEEEEEEEEEEEEEECCCCCCHHHHHHHHH
AWDDAFRAYIDQLNQHKPVIFCGDLNVAHQEIDLKNDKTNHKNAGFTDEERNKFTQLLNS
HHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
GFTDTYRYFYPSKEGVYSWWSYRFNARANNAGWRIDYFVSSKALDQKLTDAQIHTEIFGS
CCCCCEEEECCCCCCCEEEEEEEEECCCCCCCEEEEEEECCHHHHHHHCCHHEEEEECCC
DHCPVELDLDI
CCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]