Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 116493340

GI number: 116493340

Start: 1588200

End: 1590227

Strand: Reverse

Name: ligA

Synonym: PEPE_1612

Alternate gene names: 116493340

Gene position: 1590227-1588200 (Counterclockwise)

Preceding gene: 116493341

Following gene: 116493338

Centisome position: 86.78

GC content: 37.87

Gene sequence:

>2028_bases
ATGAGTTTACCAAAAAAATTGGAAGAGATGCAGGTTGAAGAAGCTAGTAAATTAGCGCAAAACCTTAGAGAGATTTTGGA
TCGTTGGAGCAAATTATATTACACCAAAGATGCTCCTGAAGTTGAAGATTACGAATATGACGAAAAATATGCGGATTTAG
TGGCGTTAGAAGAAGCTTTTCCAGAAATCATCACCCAAGATTCGATTACTCAACGAGTTGGTGGTGAAATTTTAGAGGGC
TTTACCAAAGTTACCCATACAGAACCGATGTTGTCGATGGGGGATGTTTTTTCGCGCGATGAATTAGTTGAATTTGATAA
TCGTATCCAAAAAAATGTTGGGCATCCAGTTGATTATAATGTTGAATTAAAAATTGATGGATTAGCAATTTCATTAATTT
ATCAAGATGGTGAGCTGATTCAGGGCTCTACCCGTGGAGATGGAAATATTGGTGAAGATATTACTAAAAATTTGAAGACT
ATTAAGTCAGTCCCCCAAAAATTGACCCGTCCGCTCTCGATTGAAGTTCGAGGAGAATGCTTTATGCCTAAAGCTTCGTT
TGCTAAATTGAATGCACAACAATTAGAGGATGGAAAACCAGTTTTTGCTAATCCACGTAATGCGGCAGCAGGAAGCTTAC
GACAGTTAAATACAAATGTAACTAAGAAGCGGGATTTAGACACATTTATTTATACAGTTGTGGATTCAAATCAATTAGGG
GCCAAAACTCAACATCAGGCAATTCAAATGATGGCTGAATTAGGCTTTAACACTAACCCTACCCAAGAAGTATGTGCTAA
TTTAGATGAAGTTTGGGATTATATTGCTAAATACGAAGGTCAACGGGAAGATTTACCTTATGGAATCGACGGGATCGTTT
TGAAAGTTAATGATTTAAGTCTTCAGCAAGAATTAGGACACACAGTCAAAATTCCAAGATGGGAAATTGCCTACAAATTT
CCACCAGAGGAGGCAGCTACAGTAGTTCGAGATATTGAATGGACGGTAGGACGAACAGGCGTGGTAACCCCCACAGCGGT
GATGGACCCCGTTCAATTGGCTGGAACAACAGTTAGCCGGGCCACTTTGAATAATGTAGACCAATTAACAGCTAAGGATG
TTCATATTGGCGATACGGTACTGTTACATAAAGCTGGTGATATCATTCCAGAAATTACACGAGTAGTGTTAGAAAAGCGT
CCAGTAGGAATTAGTGAGTTAGATATTCCAACCCACTGTCCATCATGTGGTAAGGAATTAGTTCACTTAAATGGCGAAGT
GGCCCTTCGTTGCATTAACCCTGATTGTCCAGCTCAAATTGTAGCTCGTTTAGAGCATTTTGGATCACGAAATGCGATGA
ATATTATGGGACTTGGACCAAAACAAATTCAACAATTATACGCGAAGAATTTTATTCATCACTTTGACGATTTATACAAA
TTAACTTCAGAGGAATTAAGCCAATTAGATGGTTTTAAAGAGAAACGTGTTAACAACTTACTTGAAGCCATTGATAATAG
CCGAAAAAATTCTTTAGAGCGTTTAATTAATGGATTAGGGATTCAAGGGGTTGGTACGAAGATGGCGCGTACTTTAGCGG
AAAAATTCGGAACGATGGATAATTTGATGCAAACAACAATTGAAGAATTTGATGCTGTAGATACGATCGGAGAAACCTTA
GCTAATAATTTAGCAACATTTTTCCAAAGTGATGTAGCCCAAAATATGATTGATGAATTGAAGGCTGTCGGAGTTAATAT
GGAATATTTGGGTGTAAAGCCAGCTGAAAGTCCTGATGGTTATTACAAAGGGAAAAAAGTTGTTTTGACTGGAAAATTAG
AACAGTATACACGAAATGAATTAAAGGAGCGTTTAATTAGTTTAGGTGCAGATGTGGCGGGTTCAGTTTCGAAGAAAACT
GATATCCTCATTGCAGGAGCCGATGCTGGTAGTAAATTAACTAAGGCACAAGCTTTAGGAATCGAAATTTTAGATGAAAC
CGAAGCTATTGCTAAATTTGAACAATAA

Upstream 100 bases:

>100_bases
ATCAAAAATTGACTGACGCCCAGATTCATACTGAAATTTTTGGTTCCGATCATTGTCCAGTCGAATTAGATCTTGATATA
TAAGTCATGGGAGAGAAAAG

Downstream 100 bases:

>100_bases
AAAAATGGGATTTAATCCCATTTTTGTTTGTTTTTGAATACGACCATTTTACTAAACGCATAGTTTGAAATGACAACAAC
GACATTATCAATAATTTTCA

Product: NAD-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 675; Mature: 674

Protein sequence:

>675_residues
MSLPKKLEEMQVEEASKLAQNLREILDRWSKLYYTKDAPEVEDYEYDEKYADLVALEEAFPEIITQDSITQRVGGEILEG
FTKVTHTEPMLSMGDVFSRDELVEFDNRIQKNVGHPVDYNVELKIDGLAISLIYQDGELIQGSTRGDGNIGEDITKNLKT
IKSVPQKLTRPLSIEVRGECFMPKASFAKLNAQQLEDGKPVFANPRNAAAGSLRQLNTNVTKKRDLDTFIYTVVDSNQLG
AKTQHQAIQMMAELGFNTNPTQEVCANLDEVWDYIAKYEGQREDLPYGIDGIVLKVNDLSLQQELGHTVKIPRWEIAYKF
PPEEAATVVRDIEWTVGRTGVVTPTAVMDPVQLAGTTVSRATLNNVDQLTAKDVHIGDTVLLHKAGDIIPEITRVVLEKR
PVGISELDIPTHCPSCGKELVHLNGEVALRCINPDCPAQIVARLEHFGSRNAMNIMGLGPKQIQQLYAKNFIHHFDDLYK
LTSEELSQLDGFKEKRVNNLLEAIDNSRKNSLERLINGLGIQGVGTKMARTLAEKFGTMDNLMQTTIEEFDAVDTIGETL
ANNLATFFQSDVAQNMIDELKAVGVNMEYLGVKPAESPDGYYKGKKVVLTGKLEQYTRNELKERLISLGADVAGSVSKKT
DILIAGADAGSKLTKAQALGIEILDETEAIAKFEQ

Sequences:

>Translated_675_residues
MSLPKKLEEMQVEEASKLAQNLREILDRWSKLYYTKDAPEVEDYEYDEKYADLVALEEAFPEIITQDSITQRVGGEILEG
FTKVTHTEPMLSMGDVFSRDELVEFDNRIQKNVGHPVDYNVELKIDGLAISLIYQDGELIQGSTRGDGNIGEDITKNLKT
IKSVPQKLTRPLSIEVRGECFMPKASFAKLNAQQLEDGKPVFANPRNAAAGSLRQLNTNVTKKRDLDTFIYTVVDSNQLG
AKTQHQAIQMMAELGFNTNPTQEVCANLDEVWDYIAKYEGQREDLPYGIDGIVLKVNDLSLQQELGHTVKIPRWEIAYKF
PPEEAATVVRDIEWTVGRTGVVTPTAVMDPVQLAGTTVSRATLNNVDQLTAKDVHIGDTVLLHKAGDIIPEITRVVLEKR
PVGISELDIPTHCPSCGKELVHLNGEVALRCINPDCPAQIVARLEHFGSRNAMNIMGLGPKQIQQLYAKNFIHHFDDLYK
LTSEELSQLDGFKEKRVNNLLEAIDNSRKNSLERLINGLGIQGVGTKMARTLAEKFGTMDNLMQTTIEEFDAVDTIGETL
ANNLATFFQSDVAQNMIDELKAVGVNMEYLGVKPAESPDGYYKGKKVVLTGKLEQYTRNELKERLISLGADVAGSVSKKT
DILIAGADAGSKLTKAQALGIEILDETEAIAKFEQ
>Mature_674_residues
SLPKKLEEMQVEEASKLAQNLREILDRWSKLYYTKDAPEVEDYEYDEKYADLVALEEAFPEIITQDSITQRVGGEILEGF
TKVTHTEPMLSMGDVFSRDELVEFDNRIQKNVGHPVDYNVELKIDGLAISLIYQDGELIQGSTRGDGNIGEDITKNLKTI
KSVPQKLTRPLSIEVRGECFMPKASFAKLNAQQLEDGKPVFANPRNAAAGSLRQLNTNVTKKRDLDTFIYTVVDSNQLGA
KTQHQAIQMMAELGFNTNPTQEVCANLDEVWDYIAKYEGQREDLPYGIDGIVLKVNDLSLQQELGHTVKIPRWEIAYKFP
PEEAATVVRDIEWTVGRTGVVTPTAVMDPVQLAGTTVSRATLNNVDQLTAKDVHIGDTVLLHKAGDIIPEITRVVLEKRP
VGISELDIPTHCPSCGKELVHLNGEVALRCINPDCPAQIVARLEHFGSRNAMNIMGLGPKQIQQLYAKNFIHHFDDLYKL
TSEELSQLDGFKEKRVNNLLEAIDNSRKNSLERLINGLGIQGVGTKMARTLAEKFGTMDNLMQTTIEEFDAVDTIGETLA
NNLATFFQSDVAQNMIDELKAVGVNMEYLGVKPAESPDGYYKGKKVVLTGKLEQYTRNELKERLISLGADVAGSVSKKTD
ILIAGADAGSKLTKAQALGIEILDETEAIAKFEQ

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=657, Percent_Identity=43.531202435312, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI87082305, Length=515, Percent_Identity=26.2135922330097, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_PEDPA (Q03DT9)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_805075.1
- ProteinModelPortal:   Q03DT9
- SMR:   Q03DT9
- STRING:   Q03DT9
- GeneID:   4417889
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1612
- NMPDR:   fig|278197.10.peg.1418
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q03DT9
- BioCyc:   PPEN278197:PEPE_1612-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74958; Mature: 74827

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 124-124 BINDING 122-122 BINDING 145-145 BINDING 179-179 BINDING 295-295 BINDING 319-319

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPKKLEEMQVEEASKLAQNLREILDRWSKLYYTKDAPEVEDYEYDEKYADLVALEEAF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHH
PEIITQDSITQRVGGEILEGFTKVTHTEPMLSMGDVFSRDELVEFDNRIQKNVGHPVDYN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCE
VELKIDGLAISLIYQDGELIQGSTRGDGNIGEDITKNLKTIKSVPQKLTRPLSIEVRGEC
EEEEECCEEEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCE
FMPKASFAKLNAQQLEDGKPVFANPRNAAAGSLRQLNTNVTKKRDLDTFIYTVVDSNQLG
ECCCHHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCHHCCCCEEEEEECCCCCC
AKTQHQAIQMMAELGFNTNPTQEVCANLDEVWDYIAKYEGQREDLPYGIDGIVLKVNDLS
CHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCH
LQQELGHTVKIPRWEIAYKFPPEEAATVVRDIEWTVGRTGVVTPTAVMDPVQLAGTTVSR
HHHHCCCEEECCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHCCHHHH
ATLNNVDQLTAKDVHIGDTVLLHKAGDIIPEITRVVLEKRPVGISELDIPTHCPSCGKEL
HHHCCHHHHHHCCEECCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
VHLNGEVALRCINPDCPAQIVARLEHFGSRNAMNIMGLGPKQIQQLYAKNFIHHFDDLYK
EECCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
LTSEELSQLDGFKEKRVNNLLEAIDNSRKNSLERLINGLGIQGVGTKMARTLAEKFGTMD
HHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHH
NLMQTTIEEFDAVDTIGETLANNLATFFQSDVAQNMIDELKAVGVNMEYLGVKPAESPDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCC
YYKGKKVVLTGKLEQYTRNELKERLISLGADVAGSVSKKTDILIAGADAGSKLTKAQALG
CCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHC
IEILDETEAIAKFEQ
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLPKKLEEMQVEEASKLAQNLREILDRWSKLYYTKDAPEVEDYEYDEKYADLVALEEAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHH
PEIITQDSITQRVGGEILEGFTKVTHTEPMLSMGDVFSRDELVEFDNRIQKNVGHPVDYN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCE
VELKIDGLAISLIYQDGELIQGSTRGDGNIGEDITKNLKTIKSVPQKLTRPLSIEVRGEC
EEEEECCEEEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCE
FMPKASFAKLNAQQLEDGKPVFANPRNAAAGSLRQLNTNVTKKRDLDTFIYTVVDSNQLG
ECCCHHHHHCCHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCHHCCCCEEEEEECCCCCC
AKTQHQAIQMMAELGFNTNPTQEVCANLDEVWDYIAKYEGQREDLPYGIDGIVLKVNDLS
CHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCH
LQQELGHTVKIPRWEIAYKFPPEEAATVVRDIEWTVGRTGVVTPTAVMDPVQLAGTTVSR
HHHHCCCEEECCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHCCHHHH
ATLNNVDQLTAKDVHIGDTVLLHKAGDIIPEITRVVLEKRPVGISELDIPTHCPSCGKEL
HHHCCHHHHHHCCEECCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
VHLNGEVALRCINPDCPAQIVARLEHFGSRNAMNIMGLGPKQIQQLYAKNFIHHFDDLYK
EECCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
LTSEELSQLDGFKEKRVNNLLEAIDNSRKNSLERLINGLGIQGVGTKMARTLAEKFGTMD
HHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHH
NLMQTTIEEFDAVDTIGETLANNLATFFQSDVAQNMIDELKAVGVNMEYLGVKPAESPDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCC
YYKGKKVVLTGKLEQYTRNELKERLISLGADVAGSVSKKTDILIAGADAGSKLTKAQALG
CCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHC
IEILDETEAIAKFEQ
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA