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The map label for this gene is holB [H]

Identifier: 116493217

GI number: 116493217

Start: 1451082

End: 1452098

Strand: Reverse

Name: holB [H]

Synonym: PEPE_1479

Alternate gene names: 116493217

Gene position: 1452098-1451082 (Counterclockwise)

Preceding gene: 116493218

Following gene: 116493216

Centisome position: 79.25

GC content: 38.25

Gene sequence:

>1017_bases
GTGGAAGATCTGAAAAATTTAACTAATCAGGCTGTGGAAAAACAACCTGAAATTGTTAAACGCTTTAATGAAATGATTGA
TAGCCAGCATTTGTCGCATGCCTACCTGTTAACCGGAGCAGGTGGGATTGGAAAAAAAGACGTCGCTCAATGGGTAGCGA
TGCGTCTTTTTTGTATTGACTTACAGGGATCAATGCCTTGTGGAGAATGTGAAGAGTGTACTCGAATCATGTCAGGACAA
CATCCAGATGTTGTAGAAATTGCACCAGATGGCCAATCAATCAAGGTGGAACAAGTTCGTTTTTTAAAATCCGAATTCTC
TAAAAGTGGGGTAGAGGGCGCACGGAAAGTTTTTATTATTGAACAAGCTAATAAAATGACAACGAGTGCGGCCAATAGTT
TGCTGAAGTTCATCGAAGAGCCTTCAGGAGAGGTAACGGCGTTTTTACTTGCTGAAAATAGAAGTTTGATGCTACCAACT
ATTGTGTCGAGAACCGAAATTATTGAACTAAAACCGTTACCTAAAGATCGATTGATTAAGGAATTGACAGAGCAAAATAT
AGCTGAAACGGATGCTAGAATTTTAACCCGGTTAACCAACGATATGAGTGAAATTCAAACACTGGTAGATAACAACTGGA
TTTTGGATGCCCATAAGGCGTTAGAAAAGTGGTTTATAACGGCCACTACTGGGGACATAACTGCATTTGTGGATGTTCAA
ACTCGAGTAATGGCTTTGGCTAAGGATCGATTTTATCAAGAAAAAATATTGGACTTGATGATGTTATATGCAATGGACCT
GTTAGAACTAAAATTTGATCAAGAAACGATTACTTATGAAGCTAATCGACGTCAGTTACAAGAGTTAGCTGATCAGACCA
ACGTCCGTCAATTATTGGCTGTTGCAGATGTATTGCTGCCTTTAAAACATCAGTTAAGTCAGAATTTAAATTTTCAAAGC
GTTGTGGAAAAAGCAACGATTAAAATATGTAATATATTCAAAATGAGAGGGCGATAG

Upstream 100 bases:

>100_bases
TGGACGTTAATATGGAAGGTACTACGGCCTATCCAATTAAGGTTCAAGTCGGTGGTGCAACCGTCCTTGTTCTTCCGGTA
GATCAATTCGAGCGCTTCTA

Downstream 100 bases:

>100_bases
TGTGGATAAAAAAGATTTATTTGATCAAATAACAGAAGTCACGCGAAATACTGAGGATTTGTTAAATAAACTACACGAAA
CCCAAAGTGCCATGGTGGAA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MEDLKNLTNQAVEKQPEIVKRFNEMIDSQHLSHAYLLTGAGGIGKKDVAQWVAMRLFCIDLQGSMPCGECEECTRIMSGQ
HPDVVEIAPDGQSIKVEQVRFLKSEFSKSGVEGARKVFIIEQANKMTTSAANSLLKFIEEPSGEVTAFLLAENRSLMLPT
IVSRTEIIELKPLPKDRLIKELTEQNIAETDARILTRLTNDMSEIQTLVDNNWILDAHKALEKWFITATTGDITAFVDVQ
TRVMALAKDRFYQEKILDLMMLYAMDLLELKFDQETITYEANRRQLQELADQTNVRQLLAVADVLLPLKHQLSQNLNFQS
VVEKATIKICNIFKMRGR

Sequences:

>Translated_338_residues
MEDLKNLTNQAVEKQPEIVKRFNEMIDSQHLSHAYLLTGAGGIGKKDVAQWVAMRLFCIDLQGSMPCGECEECTRIMSGQ
HPDVVEIAPDGQSIKVEQVRFLKSEFSKSGVEGARKVFIIEQANKMTTSAANSLLKFIEEPSGEVTAFLLAENRSLMLPT
IVSRTEIIELKPLPKDRLIKELTEQNIAETDARILTRLTNDMSEIQTLVDNNWILDAHKALEKWFITATTGDITAFVDVQ
TRVMALAKDRFYQEKILDLMMLYAMDLLELKFDQETITYEANRRQLQELADQTNVRQLLAVADVLLPLKHQLSQNLNFQS
VVEKATIKICNIFKMRGR
>Mature_338_residues
MEDLKNLTNQAVEKQPEIVKRFNEMIDSQHLSHAYLLTGAGGIGKKDVAQWVAMRLFCIDLQGSMPCGECEECTRIMSGQ
HPDVVEIAPDGQSIKVEQVRFLKSEFSKSGVEGARKVFIIEQANKMTTSAANSLLKFIEEPSGEVTAFLLAENRSLMLPT
IVSRTEIIELKPLPKDRLIKELTEQNIAETDARILTRLTNDMSEIQTLVDNNWILDAHKALEKWFITATTGDITAFVDVQ
TRVMALAKDRFYQEKILDLMMLYAMDLLELKFDQETITYEANRRQLQELADQTNVRQLLAVADVLLPLKHQLSQNLNFQS
VVEKATIKICNIFKMRGR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=145, Percent_Identity=37.2413793103448, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787341, Length=175, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 38392; Mature: 38392

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDLKNLTNQAVEKQPEIVKRFNEMIDSQHLSHAYLLTGAGGIGKKDVAQWVAMRLFCID
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHEEE
LQGSMPCGECEECTRIMSGQHPDVVEIAPDGQSIKVEQVRFLKSEFSKSGVEGARKVFII
CCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
EQANKMTTSAANSLLKFIEEPSGEVTAFLLAENRSLMLPTIVSRTEIIELKPLPKDRLIK
ECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEECHHHHCCCEEEEECCCCHHHHHH
ELTEQNIAETDARILTRLTNDMSEIQTLVDNNWILDAHKALEKWFITATTGDITAFVDVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHEEEEECCCEEEEEHHH
TRVMALAKDRFYQEKILDLMMLYAMDLLELKFDQETITYEANRRQLQELADQTNVRQLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEHHHHHHHHHHHHHHHHHHHHH
VADVLLPLKHQLSQNLNFQSVVEKATIKICNIFKMRGR
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEDLKNLTNQAVEKQPEIVKRFNEMIDSQHLSHAYLLTGAGGIGKKDVAQWVAMRLFCID
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHEEE
LQGSMPCGECEECTRIMSGQHPDVVEIAPDGQSIKVEQVRFLKSEFSKSGVEGARKVFII
CCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
EQANKMTTSAANSLLKFIEEPSGEVTAFLLAENRSLMLPTIVSRTEIIELKPLPKDRLIK
ECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEECHHHHCCCEEEEECCCCHHHHHH
ELTEQNIAETDARILTRLTNDMSEIQTLVDNNWILDAHKALEKWFITATTGDITAFVDVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHEEEEECCCEEEEEHHH
TRVMALAKDRFYQEKILDLMMLYAMDLLELKFDQETITYEANRRQLQELADQTNVRQLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEHHHHHHHHHHHHHHHHHHHHH
VADVLLPLKHQLSQNLNFQSVVEKATIKICNIFKMRGR
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]