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The map label for this gene is gatB

Identifier: 116493113

GI number: 116493113

Start: 1355588

End: 1357015

Strand: Reverse

Name: gatB

Synonym: PEPE_1368

Alternate gene names: 116493113

Gene position: 1357015-1355588 (Counterclockwise)

Preceding gene: 116493114

Following gene: 116493112

Centisome position: 74.06

GC content: 38.03

Gene sequence:

>1428_bases
ATGAATTTTAAAACAACTATTGGACTTGAAGTCCACATTGAATTAAAAACAAACTCGAAAATTTTTAGTCCTTCACCCGT
TGAATTTGGTGACCAACCTAATGCTAATACGAACGTAATTGACTGGGGATACCCGGGAGTATTACCTCAAACTAACAAGG
GTGTGGTTGAGGCAGGGATGATGGCTGCCAAAGCTTTACATGCTAATATTACTAGAACTCAACATTTCGATCGTAAAAAT
TATTTTTATCCTGATAATCCTAAGGCTTATCAAGTTACCCAAGCGGATACTCCAATTGGTACGGATGGTTGGATTGAAAT
TGAAGTAGAAGGTAAAAAGAAAAAAGTCGGTATTGCTGAGATGCATATTGAAGAAGATGCCGGTAAAAATAGTCATAATC
CCAATGGATACTCTTATGTTGATTTGAATCGTCAGGGTACTCCGTTAATTGAAATTGTTTCTAAACCAGATATCGAATCA
CCAGATGAAGCTTATGCATATCTTGAAGCGTTGCGTCAACGAATTCAATTTACTGGTATTTCAGATGTTAAGATGGAAGA
AGGATCCATGCGAGTCGACGTTAATATTTCTATTAGACCACTGGGTTCTGAAAAGTTTGGTACAAAAGCTGAATTGAAGA
ATATTAATTCCTTTAACTTTGTTCGTAAGGGACTTGCTTTTGAAGAAAAACGTCAACAAAAAATTCTGTTAGCTGGTGGA
CAAGTTCGTCCTGAAACGCGTCGTTATGATGAAGCTACGGGGCAAACTATTTTGATGCGTGTCAAAGAAGGGGCAGAAGA
TTATCGTTATTTCCCAGAACCGGATCTTCCAAGCATTACCATTGATGATGAATGGATCAAAGACGTTGAAGATAAGATGC
CAGAAATGCCTGGTAAGCGTCGTGCTCGTTACGTTGAAGAACTTGGTTTGACAGATTACGATGCGATGGTCTTAACTCAA
ACTAAGGAAATGTCAGATTTCTTTGAAGCTACTGTTGCTCAAGGAGCTGATCCTAAATTAACGGCCAACTATTTGATGGG
GGATGTTAATGCCTACTTAAATGACCAAAAAGTCGATCTTCAAGAAACCAAATTAACTCCTGAACACTTGGCCAGCATGG
TTAAAATGATTACTGATGAAACGATTTCTTCTAAGATGGCTAAGAAGGTCTTTAAAGCTATCACCCAAGGTGAAGAACCT
ACAAGTTGGGTTAAAGCTAAGGGACTGGTTCAACTATCAGACCCAGCTGTTTTGAAGCCAATGATTGTAGAAATCGTTGA
TAATAATGAACAATCTGTTGCTGACTTTAAGAATGGTAAAGATCGAGCAGTGGGCTTCTTAGTGGGACAAATTATGAAAC
AAACTAAAGGACAAGCTAACCCTAAAGTAGTTAACAAAATCCTAATGCAAGAACTTAATTCTCGTTAG

Upstream 100 bases:

>100_bases
TTGCTAAACCGTTTGATGAAGAAACGATGTATCAAGCTGGGGCAGCTTTTGAAGCTGCGACCGAGGTACATTCAAAGCAC
CCTGAAATGGAGGACAAATA

Downstream 100 bases:

>100_bases
AGGAATAGAATCGGAGGATGACGTACGATGCGGAAACGTGCGCGAATTATATATAACCCAACTTCAGGACGTGAAATGAT
TAAACAACACTTAGTTGAAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNFKTTIGLEVHIELKTNSKIFSPSPVEFGDQPNANTNVIDWGYPGVLPQTNKGVVEAGMMAAKALHANITRTQHFDRKN
YFYPDNPKAYQVTQADTPIGTDGWIEIEVEGKKKKVGIAEMHIEEDAGKNSHNPNGYSYVDLNRQGTPLIEIVSKPDIES
PDEAYAYLEALRQRIQFTGISDVKMEEGSMRVDVNISIRPLGSEKFGTKAELKNINSFNFVRKGLAFEEKRQQKILLAGG
QVRPETRRYDEATGQTILMRVKEGAEDYRYFPEPDLPSITIDDEWIKDVEDKMPEMPGKRRARYVEELGLTDYDAMVLTQ
TKEMSDFFEATVAQGADPKLTANYLMGDVNAYLNDQKVDLQETKLTPEHLASMVKMITDETISSKMAKKVFKAITQGEEP
TSWVKAKGLVQLSDPAVLKPMIVEIVDNNEQSVADFKNGKDRAVGFLVGQIMKQTKGQANPKVVNKILMQELNSR

Sequences:

>Translated_475_residues
MNFKTTIGLEVHIELKTNSKIFSPSPVEFGDQPNANTNVIDWGYPGVLPQTNKGVVEAGMMAAKALHANITRTQHFDRKN
YFYPDNPKAYQVTQADTPIGTDGWIEIEVEGKKKKVGIAEMHIEEDAGKNSHNPNGYSYVDLNRQGTPLIEIVSKPDIES
PDEAYAYLEALRQRIQFTGISDVKMEEGSMRVDVNISIRPLGSEKFGTKAELKNINSFNFVRKGLAFEEKRQQKILLAGG
QVRPETRRYDEATGQTILMRVKEGAEDYRYFPEPDLPSITIDDEWIKDVEDKMPEMPGKRRARYVEELGLTDYDAMVLTQ
TKEMSDFFEATVAQGADPKLTANYLMGDVNAYLNDQKVDLQETKLTPEHLASMVKMITDETISSKMAKKVFKAITQGEEP
TSWVKAKGLVQLSDPAVLKPMIVEIVDNNEQSVADFKNGKDRAVGFLVGQIMKQTKGQANPKVVNKILMQELNSR
>Mature_475_residues
MNFKTTIGLEVHIELKTNSKIFSPSPVEFGDQPNANTNVIDWGYPGVLPQTNKGVVEAGMMAAKALHANITRTQHFDRKN
YFYPDNPKAYQVTQADTPIGTDGWIEIEVEGKKKKVGIAEMHIEEDAGKNSHNPNGYSYVDLNRQGTPLIEIVSKPDIES
PDEAYAYLEALRQRIQFTGISDVKMEEGSMRVDVNISIRPLGSEKFGTKAELKNINSFNFVRKGLAFEEKRQQKILLAGG
QVRPETRRYDEATGQTILMRVKEGAEDYRYFPEPDLPSITIDDEWIKDVEDKMPEMPGKRRARYVEELGLTDYDAMVLTQ
TKEMSDFFEATVAQGADPKLTANYLMGDVNAYLNDQKVDLQETKLTPEHLASMVKMITDETISSKMAKKVFKAITQGEEP
TSWVKAKGLVQLSDPAVLKPMIVEIVDNNEQSVADFKNGKDRAVGFLVGQIMKQTKGQANPKVVNKILMQELNSR

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=33.9393939393939, Blast_Score=288, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI115534094, Length=375, Percent_Identity=33.6, Blast_Score=206, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6319391, Length=521, Percent_Identity=27.2552783109405, Blast_Score=196, Evalue=9e-51,
Organism=Drosophila melanogaster, GI45550808, Length=482, Percent_Identity=30.4979253112033, Blast_Score=251, Evalue=8e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_PEDPA (Q03EG6)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804848.1
- ProteinModelPortal:   Q03EG6
- SMR:   Q03EG6
- STRING:   Q03EG6
- GeneID:   4416983
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1368
- NMPDR:   fig|278197.10.peg.1202
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q03EG6
- ProtClustDB:   PRK05477
- BioCyc:   PPEN278197:PEPE_1368-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53366; Mature: 53366

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFKTTIGLEVHIELKTNSKIFSPSPVEFGDQPNANTNVIDWGYPGVLPQTNKGVVEAGM
CCCEEEEEEEEEEEEECCCEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHH
MAAKALHANITRTQHFDRKNYFYPDNPKAYQVTQADTPIGTDGWIEIEVEGKKKKVGIAE
HHHHHHHHCCHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCEEE
MHIEEDAGKNSHNPNGYSYVDLNRQGTPLIEIVSKPDIESPDEAYAYLEALRQRIQFTGI
EEEECCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
SDVKMEEGSMRVDVNISIRPLGSEKFGTKAELKNINSFNFVRKGLAFEEKRQQKILLAGG
CCEEECCCCEEEEEEEEEEECCCCCCCCHHHHHCCCHHHHHHHCCCHHHHHCCEEEEECC
QVRPETRRYDEATGQTILMRVKEGAEDYRYFPEPDLPSITIDDEWIKDVEDKMPEMPGKR
CCCCHHHHHHHHCCCEEEEEEHHCCHHCCCCCCCCCCEEEECHHHHHHHHHHCCCCCCHH
RARYVEELGLTDYDAMVLTQTKEMSDFFEATVAQGADPKLTANYLMGDVNAYLNDQKVDL
HHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCCH
QETKLTPEHLASMVKMITDETISSKMAKKVFKAITQGEEPTSWVKAKGLVQLSDPAVLKP
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEECCCCHHHH
MIVEIVDNNEQSVADFKNGKDRAVGFLVGQIMKQTKGQANPKVVNKILMQELNSR
HEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFKTTIGLEVHIELKTNSKIFSPSPVEFGDQPNANTNVIDWGYPGVLPQTNKGVVEAGM
CCCEEEEEEEEEEEEECCCEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHH
MAAKALHANITRTQHFDRKNYFYPDNPKAYQVTQADTPIGTDGWIEIEVEGKKKKVGIAE
HHHHHHHHCCHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCEEE
MHIEEDAGKNSHNPNGYSYVDLNRQGTPLIEIVSKPDIESPDEAYAYLEALRQRIQFTGI
EEEECCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
SDVKMEEGSMRVDVNISIRPLGSEKFGTKAELKNINSFNFVRKGLAFEEKRQQKILLAGG
CCEEECCCCEEEEEEEEEEECCCCCCCCHHHHHCCCHHHHHHHCCCHHHHHCCEEEEECC
QVRPETRRYDEATGQTILMRVKEGAEDYRYFPEPDLPSITIDDEWIKDVEDKMPEMPGKR
CCCCHHHHHHHHCCCEEEEEEHHCCHHCCCCCCCCCCEEEECHHHHHHHHHHCCCCCCHH
RARYVEELGLTDYDAMVLTQTKEMSDFFEATVAQGADPKLTANYLMGDVNAYLNDQKVDL
HHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCCH
QETKLTPEHLASMVKMITDETISSKMAKKVFKAITQGEEPTSWVKAKGLVQLSDPAVLKP
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEECCCCHHHH
MIVEIVDNNEQSVADFKNGKDRAVGFLVGQIMKQTKGQANPKVVNKILMQELNSR
HEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA