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The map label for this gene is gatA

Identifier: 116493114

GI number: 116493114

Start: 1357015

End: 1358475

Strand: Reverse

Name: gatA

Synonym: PEPE_1369

Alternate gene names: 116493114

Gene position: 1358475-1357015 (Counterclockwise)

Preceding gene: 116493115

Following gene: 116493113

Centisome position: 74.14

GC content: 38.6

Gene sequence:

>1461_bases
ATGAAGTTTTTTGATATAACAGTAACAGAGCTTCACCAAAAGTTAGTTGATAAAGAATTAACAGTAACTGAATTAGTTTC
AGAAACATTGGATCAAATTGAAGCCGACCAAAAAGTTTTAAATGATTTTGTGACCATTAATCGCGAGGGAGCTTTAGCTC
AAGCCCGTGCTATCGATGAAAAGGGAATTAACCCTGATAATATTTGGGCCGGAATCCCGATTGCTATCAAAGACAATATT
GTAACCAAAGGAATCAAAACTACTGCTGCATCTAAGATGCTCAGTAACTTCACACCCATCTACGATGCAACTGTTATTGA
ACGTTTACAAGCTAATGATGTAATTATTATTGGTAAGACAAATATGGATGAATTTGCCATGGGTGGATCAACTGAAACTT
CATACTTTGGTGATACGCGTAATCCATGGGATCATACTAAGGTTCCTGGTGGGTCATCAGGTGGTTCTGCTACAACAGTT
TCAGCTGGAAATTCAATTGCTGCTCTTGGTTCAGACACGGGTGGCTCAATTCGTCAACCAGCCTCATACACTGGAATTGT
TGGGGTGAAACCAACTTATGGACGTATCTCACGTTATGGTTTAATTGCTTTTGGATCAAGTTTAGATCAAATTGGACCCA
TGACGCGTACAGTTAAGGATAATGCAGCTTTATTGAATATTATTGCTGGAATGGATGAGCGCGATTTAACTAGTTCTGAC
AAAAAGGTACCAGACTTCACATCTAAGATTGGTAAAGATATTAAGGGGATGAAGATTGGTTTGCCTAAAGAATATCTAGG
CGATGGAATCAATGAAGACGTTAAAAAAGTCGTACGTCAGGCAGTTGAAACATTAACCAATTTGGGAGCAACGATTGAAG
AAATTTCGCTCCCACGGAGTAAGTATGGTGTAGCAGCTTATTATATTATCGGTTCTTCAGAAGCTTCATCTAACTTACAA
CGCTTCGATGGTATTAGATATGGTCATCGAGCTCAAGATGTCAAAAATCTTGAAGATGTATATGTTAAATCACGTTCCGA
AGGTTTCGGAGATGAAGTAAAGCGTCGTATCATGTTAGGGACTTATTCGTTATCGGCTGGTACATATGATGCATACTTTA
AGAAGGCAGCCCAAGTTCGTACTTTAATTATTAATGATTTCAATAAAATCTTTGAAGATTATGATTTGATTATTGGACCA
ACAGCTCCAACACCAGCCTACGCTATAGGCGCTGAAGTTGATGATCCAACTACAATGTATATGAATGATGTTTTAACAAT
CCCAGTTAACTTAGCAGGTTTACCAGCAATGTCAGTTCCTGCTGGATTTAGTGATAATATGCCAGTCGGATTACAAATTA
TTGCTAAACCGTTTGATGAAGAAACGATGTATCAAGCTGGGGCAGCTTTTGAAGCTGCGACCGAGGTACATTCAAAGCAC
CCTGAAATGGAGGACAAATAA

Upstream 100 bases:

>100_bases
TTCGTTCGAATGATAAGGAAGCATTATTAGCTAATGCCCCTGAAACAGAAAACGGATTTATCCGTGTACCAGCAATTCTT
GATGAAAGTGGGGAAGCATA

Downstream 100 bases:

>100_bases
TGAATTTTAAAACAACTATTGGACTTGAAGTCCACATTGAATTAAAAACAAACTCGAAAATTTTTAGTCCTTCACCCGTT
GAATTTGGTGACCAACCTAA

Product: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MKFFDITVTELHQKLVDKELTVTELVSETLDQIEADQKVLNDFVTINREGALAQARAIDEKGINPDNIWAGIPIAIKDNI
VTKGIKTTAASKMLSNFTPIYDATVIERLQANDVIIIGKTNMDEFAMGGSTETSYFGDTRNPWDHTKVPGGSSGGSATTV
SAGNSIAALGSDTGGSIRQPASYTGIVGVKPTYGRISRYGLIAFGSSLDQIGPMTRTVKDNAALLNIIAGMDERDLTSSD
KKVPDFTSKIGKDIKGMKIGLPKEYLGDGINEDVKKVVRQAVETLTNLGATIEEISLPRSKYGVAAYYIIGSSEASSNLQ
RFDGIRYGHRAQDVKNLEDVYVKSRSEGFGDEVKRRIMLGTYSLSAGTYDAYFKKAAQVRTLIINDFNKIFEDYDLIIGP
TAPTPAYAIGAEVDDPTTMYMNDVLTIPVNLAGLPAMSVPAGFSDNMPVGLQIIAKPFDEETMYQAGAAFEAATEVHSKH
PEMEDK

Sequences:

>Translated_486_residues
MKFFDITVTELHQKLVDKELTVTELVSETLDQIEADQKVLNDFVTINREGALAQARAIDEKGINPDNIWAGIPIAIKDNI
VTKGIKTTAASKMLSNFTPIYDATVIERLQANDVIIIGKTNMDEFAMGGSTETSYFGDTRNPWDHTKVPGGSSGGSATTV
SAGNSIAALGSDTGGSIRQPASYTGIVGVKPTYGRISRYGLIAFGSSLDQIGPMTRTVKDNAALLNIIAGMDERDLTSSD
KKVPDFTSKIGKDIKGMKIGLPKEYLGDGINEDVKKVVRQAVETLTNLGATIEEISLPRSKYGVAAYYIIGSSEASSNLQ
RFDGIRYGHRAQDVKNLEDVYVKSRSEGFGDEVKRRIMLGTYSLSAGTYDAYFKKAAQVRTLIINDFNKIFEDYDLIIGP
TAPTPAYAIGAEVDDPTTMYMNDVLTIPVNLAGLPAMSVPAGFSDNMPVGLQIIAKPFDEETMYQAGAAFEAATEVHSKH
PEMEDK
>Mature_486_residues
MKFFDITVTELHQKLVDKELTVTELVSETLDQIEADQKVLNDFVTINREGALAQARAIDEKGINPDNIWAGIPIAIKDNI
VTKGIKTTAASKMLSNFTPIYDATVIERLQANDVIIIGKTNMDEFAMGGSTETSYFGDTRNPWDHTKVPGGSSGGSATTV
SAGNSIAALGSDTGGSIRQPASYTGIVGVKPTYGRISRYGLIAFGSSLDQIGPMTRTVKDNAALLNIIAGMDERDLTSSD
KKVPDFTSKIGKDIKGMKIGLPKEYLGDGINEDVKKVVRQAVETLTNLGATIEEISLPRSKYGVAAYYIIGSSEASSNLQ
RFDGIRYGHRAQDVKNLEDVYVKSRSEGFGDEVKRRIMLGTYSLSAGTYDAYFKKAAQVRTLIINDFNKIFEDYDLIIGP
TAPTPAYAIGAEVDDPTTMYMNDVLTIPVNLAGLPAMSVPAGFSDNMPVGLQIIAKPFDEETMYQAGAAFEAATEVHSKH
PEMEDK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=496, Percent_Identity=36.8951612903226, Blast_Score=322, Evalue=7e-88,
Organism=Homo sapiens, GI195972892, Length=475, Percent_Identity=24.2105263157895, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI166795287, Length=153, Percent_Identity=34.640522875817, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=428, Percent_Identity=38.5514018691589, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17537465, Length=507, Percent_Identity=24.65483234714, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=282, Percent_Identity=26.9503546099291, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=312, Percent_Identity=26.6025641025641, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=241, Percent_Identity=28.2157676348548, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=234, Percent_Identity=29.0598290598291, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=234, Percent_Identity=29.0598290598291, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=239, Percent_Identity=29.2887029288703, Blast_Score=79, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=460, Percent_Identity=35.2173913043478, Blast_Score=250, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6319685, Length=426, Percent_Identity=29.5774647887324, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6320448, Length=295, Percent_Identity=28.135593220339, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648113, Length=496, Percent_Identity=39.5161290322581, Blast_Score=327, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24652985, Length=494, Percent_Identity=27.1255060728745, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI19922090, Length=494, Percent_Identity=27.1255060728745, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24652981, Length=494, Percent_Identity=27.1255060728745, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24652983, Length=494, Percent_Identity=27.1255060728745, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24644968, Length=494, Percent_Identity=26.3157894736842, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078093, Length=498, Percent_Identity=24.8995983935743, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648435, Length=478, Percent_Identity=24.6861924686192, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648437, Length=478, Percent_Identity=24.6861924686192, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI45550774, Length=478, Percent_Identity=24.6861924686192, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356731, Length=496, Percent_Identity=25.2016129032258, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648441, Length=401, Percent_Identity=25.4364089775561, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648439, Length=401, Percent_Identity=25.4364089775561, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_PEDPA (Q03EG5)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804849.1
- ProteinModelPortal:   Q03EG5
- SMR:   Q03EG5
- STRING:   Q03EG5
- GeneID:   4416984
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1369
- NMPDR:   fig|278197.10.peg.1203
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   Q03EG5
- BioCyc:   PPEN278197:PEPE_1369-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52636; Mature: 52636

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 77-77 ACT_SITE 152-152 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFFDITVTELHQKLVDKELTVTELVSETLDQIEADQKVLNDFVTINREGALAQARAIDE
CCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHH
KGINPDNIWAGIPIAIKDNIVTKGIKTTAASKMLSNFTPIYDATVIERLQANDVIIIGKT
CCCCCCCEEECCCEEEECCHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECC
NMDEFAMGGSTETSYFGDTRNPWDHTKVPGGSSGGSATTVSAGNSIAALGSDTGGSIRQP
CCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCC
ASYTGIVGVKPTYGRISRYGLIAFGSSLDQIGPMTRTVKDNAALLNIIAGMDERDLTSSD
CCCCCEEECCCCHHHHHHCEEEEECCCHHHHCCCHHHHCCCHHHHHHHHCCCCCCCCCCC
KKVPDFTSKIGKDIKGMKIGLPKEYLGDGINEDVKKVVRQAVETLTNLGATIEEISLPRS
CCCCHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCH
KYGVAAYYIIGSSEASSNLQRFDGIRYGHRAQDVKNLEDVYVKSRSEGFGDEVKRRIMLG
HCCEEEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEE
TYSLSAGTYDAYFKKAAQVRTLIINDFNKIFEDYDLIIGPTAPTPAYAIGAEVDDPTTMY
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEECCCCCCCCEEE
MNDVLTIPVNLAGLPAMSVPAGFSDNMPVGLQIIAKPFDEETMYQAGAAFEAATEVHSKH
ECCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHCC
PEMEDK
CCCCCC
>Mature Secondary Structure
MKFFDITVTELHQKLVDKELTVTELVSETLDQIEADQKVLNDFVTINREGALAQARAIDE
CCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHH
KGINPDNIWAGIPIAIKDNIVTKGIKTTAASKMLSNFTPIYDATVIERLQANDVIIIGKT
CCCCCCCEEECCCEEEECCHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECC
NMDEFAMGGSTETSYFGDTRNPWDHTKVPGGSSGGSATTVSAGNSIAALGSDTGGSIRQP
CCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCC
ASYTGIVGVKPTYGRISRYGLIAFGSSLDQIGPMTRTVKDNAALLNIIAGMDERDLTSSD
CCCCCEEECCCCHHHHHHCEEEEECCCHHHHCCCHHHHCCCHHHHHHHHCCCCCCCCCCC
KKVPDFTSKIGKDIKGMKIGLPKEYLGDGINEDVKKVVRQAVETLTNLGATIEEISLPRS
CCCCHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCH
KYGVAAYYIIGSSEASSNLQRFDGIRYGHRAQDVKNLEDVYVKSRSEGFGDEVKRRIMLG
HCCEEEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEE
TYSLSAGTYDAYFKKAAQVRTLIINDFNKIFEDYDLIIGPTAPTPAYAIGAEVDDPTTMY
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEECCCCCCCCEEE
MNDVLTIPVNLAGLPAMSVPAGFSDNMPVGLQIIAKPFDEETMYQAGAAFEAATEVHSKH
ECCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHCC
PEMEDK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA