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The map label for this gene is dagK [H]

Identifier: 116493112

GI number: 116493112

Start: 1354559

End: 1355560

Strand: Reverse

Name: dagK [H]

Synonym: PEPE_1367

Alternate gene names: 116493112

Gene position: 1355560-1354559 (Counterclockwise)

Preceding gene: 116493113

Following gene: 116493111

Centisome position: 73.98

GC content: 38.82

Gene sequence:

>1002_bases
ATGCGGAAACGTGCGCGAATTATATATAACCCAACTTCAGGACGTGAAATGATTAAACAACACTTAGTTGAAATTCTTGC
GGTAATTGAGAAAGCAGGATATGAGACAAGTGCTTTTGCTACTACACCTGAACCCGATTCTGCCAAAAACGAGGCTCGCC
GAGCTGCAAAAGATGGATTTGATTTAGTGGTTGCAGCTGGCGGAGACGGAACGATTAATGAAGTAGTAAATGGAATTGCA
CCGTTACTTAGACGTCCTAAAATGGCGATTATCCCGACTGGAACGACCAATGATTATGCGCGGGCTTTACGAATTCCTCG
CGAAGATCCAGTGGGTGCTGCCAAAGTTATTTTGCAAAATCAACGACTAAAGATGGATATTGGTAAAGCTGGCGAAAATT
ATTTCATTAATATTGCGGCCGGTGGTTTATTAACGGAACTAACTTATGATGTGCCTTCAAACCTTAAAAGTATTTTTGGC
TATCTTGCATATATTGTTAAGGGGGCAGAATTACTACCACGGGTAAAACCGGTCGAAATGGACATCGAATATGATGGAGA
ACGGTATCATGGTAAAGCTTCCATGTTTTTCCTCGCGTTGACGAATTCAATTGGTGGATTTGAACAAATTGTACCGGATG
CCTCTCTGGATGACGGTAAATTTACAATGATTATTGTAAAATCAGGTAATTTACCTAACTTACTCCATTTAATGGTTAAA
GCCTTAAATGGAAAACATGTCAATGATTCGCGAATCATTTATCGGAAAGCACGAAAAGTTACGGTCCGTCCAGTAAACGA
TCGTGTTATGGTTAATTTGGATGGGGAGTATGGTGGAGATGCTCCTATGGTATTTAAAAATTTAAAACGCCATATTGAAA
TGTTCACTAACTTAGATGATGTACCAGTTAATTTGATTAAAGAAGATGACGAATATCGACAAGTGGAACAACAATTTGTC
GAAGAAGTTCAAAATCTTCCAGATGATAACAAAATAGATTAA

Upstream 100 bases:

>100_bases
GAAACAAACTAAAGGACAAGCTAACCCTAAAGTAGTTAACAAAATCCTAATGCAAGAACTTAATTCTCGTTAGAGGAATA
GAATCGGAGGATGACGTACG

Downstream 100 bases:

>100_bases
AGAAAAGCTTGAGAAATACGCTGAAATGCGTTACAGTTCTTGAGTAGCACACGAGAATAAAACAGGCTGGTATTAATGTG
CCAGCCTGTTTTTATCAGTA

Product: putative lipid kinase

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MRKRARIIYNPTSGREMIKQHLVEILAVIEKAGYETSAFATTPEPDSAKNEARRAAKDGFDLVVAAGGDGTINEVVNGIA
PLLRRPKMAIIPTGTTNDYARALRIPREDPVGAAKVILQNQRLKMDIGKAGENYFINIAAGGLLTELTYDVPSNLKSIFG
YLAYIVKGAELLPRVKPVEMDIEYDGERYHGKASMFFLALTNSIGGFEQIVPDASLDDGKFTMIIVKSGNLPNLLHLMVK
ALNGKHVNDSRIIYRKARKVTVRPVNDRVMVNLDGEYGGDAPMVFKNLKRHIEMFTNLDDVPVNLIKEDDEYRQVEQQFV
EEVQNLPDDNKID

Sequences:

>Translated_333_residues
MRKRARIIYNPTSGREMIKQHLVEILAVIEKAGYETSAFATTPEPDSAKNEARRAAKDGFDLVVAAGGDGTINEVVNGIA
PLLRRPKMAIIPTGTTNDYARALRIPREDPVGAAKVILQNQRLKMDIGKAGENYFINIAAGGLLTELTYDVPSNLKSIFG
YLAYIVKGAELLPRVKPVEMDIEYDGERYHGKASMFFLALTNSIGGFEQIVPDASLDDGKFTMIIVKSGNLPNLLHLMVK
ALNGKHVNDSRIIYRKARKVTVRPVNDRVMVNLDGEYGGDAPMVFKNLKRHIEMFTNLDDVPVNLIKEDDEYRQVEQQFV
EEVQNLPDDNKID
>Mature_333_residues
MRKRARIIYNPTSGREMIKQHLVEILAVIEKAGYETSAFATTPEPDSAKNEARRAAKDGFDLVVAAGGDGTINEVVNGIA
PLLRRPKMAIIPTGTTNDYARALRIPREDPVGAAKVILQNQRLKMDIGKAGENYFINIAAGGLLTELTYDVPSNLKSIFG
YLAYIVKGAELLPRVKPVEMDIEYDGERYHGKASMFFLALTNSIGGFEQIVPDASLDDGKFTMIIVKSGNLPNLLHLMVK
ALNGKHVNDSRIIYRKARKVTVRPVNDRVMVNLDGEYGGDAPMVFKNLKRHIEMFTNLDDVPVNLIKEDDEYRQVEQQFV
EEVQNLPDDNKID

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=281, Percent_Identity=29.1814946619217, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 37117; Mature: 37117

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKRARIIYNPTSGREMIKQHLVEILAVIEKAGYETSAFATTPEPDSAKNEARRAAKDGF
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHCCCC
DLVVAAGGDGTINEVVNGIAPLLRRPKMAIIPTGTTNDYARALRIPREDPVGAAKVILQN
EEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHCC
QRLKMDIGKAGENYFINIAAGGLLTELTYDVPSNLKSIFGYLAYIVKGAELLPRVKPVEM
CCEEEECCCCCCCEEEEEECCCEEHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
DIEYDGERYHGKASMFFLALTNSIGGFEQIVPDASLDDGKFTMIIVKSGNLPNLLHLMVK
EEEECCCEECCCCEEEEEEECCCCCCHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHH
ALNGKHVNDSRIIYRKARKVTVRPVNDRVMVNLDGEYGGDAPMVFKNLKRHIEMFTNLDD
HHCCCCCCCCHHEEECCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCC
VPVNLIKEDDEYRQVEQQFVEEVQNLPDDNKID
CCHHEECCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRKRARIIYNPTSGREMIKQHLVEILAVIEKAGYETSAFATTPEPDSAKNEARRAAKDGF
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHCCCC
DLVVAAGGDGTINEVVNGIAPLLRRPKMAIIPTGTTNDYARALRIPREDPVGAAKVILQN
EEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHCC
QRLKMDIGKAGENYFINIAAGGLLTELTYDVPSNLKSIFGYLAYIVKGAELLPRVKPVEM
CCEEEECCCCCCCEEEEEECCCEEHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
DIEYDGERYHGKASMFFLALTNSIGGFEQIVPDASLDDGKFTMIIVKSGNLPNLLHLMVK
EEEECCCEECCCCEEEEEEECCCCCCHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHH
ALNGKHVNDSRIIYRKARKVTVRPVNDRVMVNLDGEYGGDAPMVFKNLKRHIEMFTNLDD
HHCCCCCCCCHHEEECCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCC
VPVNLIKEDDEYRQVEQQFVEEVQNLPDDNKID
CCHHEECCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]