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The map label for this gene is iscS [C]

Identifier: 116493042

GI number: 116493042

Start: 1286173

End: 1287327

Strand: Reverse

Name: iscS [C]

Synonym: PEPE_1294

Alternate gene names: 116493042

Gene position: 1287327-1286173 (Counterclockwise)

Preceding gene: 116493043

Following gene: 116493041

Centisome position: 70.25

GC content: 37.14

Gene sequence:

>1155_bases
ATGATTTATTTTGATAATAGTGCAACAACTAAAGTTGATAAGAACGTTTTAGAGACCTACCAAAAGGTCAGTAATAAGAT
TTGGGGTAACCCTTCTAGTTTGCATGATATGGGCGAAATGTCGTTCAATTTATTGGAGCAGTCTAGAAAACAAATTGCTA
GTTTGTTAAATGTTAAACCTCACGAAATATTTTTTACAAGTGGTGGAACGGAAGGCGACAACTGGGTAATTAAAGGGACA
GCGATGGAAAAACGGATGTATGGAAAGCATCTAATTACGACGGCTGTCGAACATCCTGCCGTAAAAAATTCTATGGAACA
GTTAGCACAATTAGGATTTGATATTACCTATCTTCCCGTTGATGATGAGGGTCGAATTAACATTCAAGATTTAAAAGATG
CCATTCGCCCGGATACGATTCTGGTTTCGATTATGGCAATTAATAATGAAATTGGAACAATTCAACCACTTTTAAAAGCA
GCAGAAGTTTTAAAGGACTATCCAAAAGTACATTTTCATATCGATGCTGTTCAAGGAATTGGTAAAGGGATTCAAGCTGC
AATCATGAATGATCGTGTTGATTTTGTAACCTTCTCTGGCCATAAATTTCATGCTCCTCGAGGGGTGGGTTTTATTTATG
CGCGCGAAGGTAGGCGAATTGCACCATTGTTAACTGGTGGTGGACAGGAAAAGAATTTACGGAGTGGAACGGAGAACTTA
CCTGCGATTGCTGGGATGGCTAAGGCACTGCGTTTACTACTTGATGGCGAAGATCAAAAGGTAGCTTTACAAGAATCAAT
TAAAGAGAAAATTTATAATCACGTAACTAAGTTTGAAAAAGTGGTTGCTTTTTCTAAAATTCAAGCTAACTTTGCTCCGC
ATATTTTGTGTTTTGCAATTAAAGGGGTGCGTGGAGAAACGATTGTTCATGCGTTTGAATCACATGGAATTTACATTTCG
ACCACTAGTGCTTGTTCTTCTAAAAAACAGCAAGCTTCAAGTACTTTAACGGCAATGAAGATTGACTCAACCATTTCAAC
GAGTGCCGTCCGAGTTAGCTTAGATAGTGCTAATTCTTTGGAAGAAGCTGATGAATTTAATCGAATTTTTGACCAATTAT
ATCAAGACTTTTCAAAAATAAATAAAAATAGCTAG

Upstream 100 bases:

>100_bases
CTTTGAAGATCTAAATATTTAGATTACTTTAATTTTGTAAGTATATTTTTCTGTTATAATTATTAGGGTTTACGGAAATA
TATAATGGAGAAATGAGTAA

Downstream 100 bases:

>100_bases
GAGTTTATATGAAATATACGGAAATAATGGTTCGCTACGGTGAACTATCAACTAAAGGTAAAAACCGCAAAGATTTTATT
GCTCGTTTGGGAGGAAATAT

Product: cysteine sulfinate desulfinase/cysteine desulfurase

Products: L-alanine; S [C]

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MIYFDNSATTKVDKNVLETYQKVSNKIWGNPSSLHDMGEMSFNLLEQSRKQIASLLNVKPHEIFFTSGGTEGDNWVIKGT
AMEKRMYGKHLITTAVEHPAVKNSMEQLAQLGFDITYLPVDDEGRINIQDLKDAIRPDTILVSIMAINNEIGTIQPLLKA
AEVLKDYPKVHFHIDAVQGIGKGIQAAIMNDRVDFVTFSGHKFHAPRGVGFIYAREGRRIAPLLTGGGQEKNLRSGTENL
PAIAGMAKALRLLLDGEDQKVALQESIKEKIYNHVTKFEKVVAFSKIQANFAPHILCFAIKGVRGETIVHAFESHGIYIS
TTSACSSKKQQASSTLTAMKIDSTISTSAVRVSLDSANSLEEADEFNRIFDQLYQDFSKINKNS

Sequences:

>Translated_384_residues
MIYFDNSATTKVDKNVLETYQKVSNKIWGNPSSLHDMGEMSFNLLEQSRKQIASLLNVKPHEIFFTSGGTEGDNWVIKGT
AMEKRMYGKHLITTAVEHPAVKNSMEQLAQLGFDITYLPVDDEGRINIQDLKDAIRPDTILVSIMAINNEIGTIQPLLKA
AEVLKDYPKVHFHIDAVQGIGKGIQAAIMNDRVDFVTFSGHKFHAPRGVGFIYAREGRRIAPLLTGGGQEKNLRSGTENL
PAIAGMAKALRLLLDGEDQKVALQESIKEKIYNHVTKFEKVVAFSKIQANFAPHILCFAIKGVRGETIVHAFESHGIYIS
TTSACSSKKQQASSTLTAMKIDSTISTSAVRVSLDSANSLEEADEFNRIFDQLYQDFSKINKNS
>Mature_384_residues
MIYFDNSATTKVDKNVLETYQKVSNKIWGNPSSLHDMGEMSFNLLEQSRKQIASLLNVKPHEIFFTSGGTEGDNWVIKGT
AMEKRMYGKHLITTAVEHPAVKNSMEQLAQLGFDITYLPVDDEGRINIQDLKDAIRPDTILVSIMAINNEIGTIQPLLKA
AEVLKDYPKVHFHIDAVQGIGKGIQAAIMNDRVDFVTFSGHKFHAPRGVGFIYAREGRRIAPLLTGGGQEKNLRSGTENL
PAIAGMAKALRLLLDGEDQKVALQESIKEKIYNHVTKFEKVVAFSKIQANFAPHILCFAIKGVRGETIVHAFESHGIYIS
TTSACSSKKQQASSTLTAMKIDSTISTSAVRVSLDSANSLEEADEFNRIFDQLYQDFSKINKNS

Specific function: Fe-S cluster biosynthesis [C]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=366, Percent_Identity=35.792349726776, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI156713448, Length=402, Percent_Identity=30.0995024875622, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI48994898, Length=368, Percent_Identity=35.8695652173913, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1789175, Length=346, Percent_Identity=23.6994219653179, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787970, Length=397, Percent_Identity=21.6624685138539, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25143064, Length=387, Percent_Identity=32.8165374677003, Blast_Score=197, Evalue=9e-51,
Organism=Caenorhabditis elegans, GI17533177, Length=337, Percent_Identity=29.673590504451, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319831, Length=366, Percent_Identity=36.8852459016393, Blast_Score=238, Evalue=2e-63,
Organism=Drosophila melanogaster, GI20129463, Length=372, Percent_Identity=35.2150537634409, Blast_Score=211, Evalue=7e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: NA

Molecular weight: Translated: 42536; Mature: 42536

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYFDNSATTKVDKNVLETYQKVSNKIWGNPSSLHDMGEMSFNLLEQSRKQIASLLNVKP
CEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HEIFFTSGGTEGDNWVIKGTAMEKRMYGKHLITTAVEHPAVKNSMEQLAQLGFDITYLPV
CEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEE
DDEGRINIQDLKDAIRPDTILVSIMAINNEIGTIQPLLKAAEVLKDYPKVHFHIDAVQGI
CCCCCEEHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHC
GKGIQAAIMNDRVDFVTFSGHKFHAPRGVGFIYAREGRRIAPLLTGGGQEKNLRSGTENL
CCCHHHHHHCCCEEEEEECCCEEECCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCC
PAIAGMAKALRLLLDGEDQKVALQESIKEKIYNHVTKFEKVVAFSKIQANFAPHILCFAI
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
KGVRGETIVHAFESHGIYISTTSACSSKKQQASSTLTAMKIDSTISTSAVRVSLDSANSL
CCCCCCHHHHHHHCCCEEEEECHHHHHHHHHHHHHHEEEEECCCCCCCEEEEEECCCCCH
EEADEFNRIFDQLYQDFSKINKNS
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIYFDNSATTKVDKNVLETYQKVSNKIWGNPSSLHDMGEMSFNLLEQSRKQIASLLNVKP
CEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HEIFFTSGGTEGDNWVIKGTAMEKRMYGKHLITTAVEHPAVKNSMEQLAQLGFDITYLPV
CEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEE
DDEGRINIQDLKDAIRPDTILVSIMAINNEIGTIQPLLKAAEVLKDYPKVHFHIDAVQGI
CCCCCEEHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHC
GKGIQAAIMNDRVDFVTFSGHKFHAPRGVGFIYAREGRRIAPLLTGGGQEKNLRSGTENL
CCCHHHHHHCCCEEEEEECCCEEECCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCC
PAIAGMAKALRLLLDGEDQKVALQESIKEKIYNHVTKFEKVVAFSKIQANFAPHILCFAI
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
KGVRGETIVHAFESHGIYISTTSACSSKKQQASSTLTAMKIDSTISTSAVRVSLDSANSL
CCCCCCHHHHHHHCCCEEEEECHHHHHHHHHHHHHHEEEEECCCCCCCEEEEEECCCCCH
EEADEFNRIFDQLYQDFSKINKNS
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-cysteine [C]

Specific reaction: L-cysteine = L-alanine + S [C]

General reaction: transfers sulfane sulfur to ThiI during in vitro biosynthesis of 4-thiouridine in E. coli tRNA [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8031904 [H]