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The map label for this gene is thiI

Identifier: 116493041

GI number: 116493041

Start: 1284947

End: 1286164

Strand: Reverse

Name: thiI

Synonym: PEPE_1293

Alternate gene names: 116493041

Gene position: 1286164-1284947 (Counterclockwise)

Preceding gene: 116493042

Following gene: 116493040

Centisome position: 70.19

GC content: 36.7

Gene sequence:

>1218_bases
ATGAAATATACGGAAATAATGGTTCGCTACGGTGAACTATCAACTAAAGGTAAAAACCGCAAAGATTTTATTGCTCGTTT
GGGAGGAAATATACGTAAAAGTCTTAAGGACTTTGAAGACGTTGAAATTCATCCTAATCGTGATCGTACACATGTAACCT
TAAATGGTACAGACAGTGATGCAGTCATTAACAGACTTAAAAAAGTTTTTGGGATTCAAAATTTTTCGCCAATGTTAAAA
GTTGAGAAAACAATGGAAGCTGTCCAAGCTGGTGCTCTAGAAATGATGAAGGAGCAATTAAAGCCGGGAATGACCTTTAA
AATTAATACACGTCGTTCTGATAAAGAATTTGCTATTAATACAGATACCATGAATCGGGAACTTGGTGGATTTATACTAG
ATAACTTCCCAGATAATGATGTAAAAATGAAAAATCCAGACATTACCTTACGTGTTGAAATTCGATCTAATGGAATTTTC
TTAACTAGTGAAGTAATTAATGGGGCAGGTGGCTTACCGGTTGGAACTGCTGGTAAGGGAATGATGATGCTTTCTGGTGG
AATTGATTCTCCTGTGGCAGGTTATTTGGGGATGAAACGTGGCGTAGAGATGGAAATGGTGCATTTTTTCAGTCCGCCAT
ATACTAGCGAACAAGCTCTAGCTAAGGCTAAGGAATTAAGTGGAAAATTAGCTGCATATTCTGGAAGTGTTCAATTTATC
CAAGTTCCATTTACAGAAATTCAAGAAACTATTAAAGAAAAATGTCCAGAGGGGTATTTGATGACGATTCAACGTCGAAT
GATGTTGCGCCTAGTAGTTGCCCTAGCTAAACAACGTGGTGGACTAGCAATCTTTAATGGAGAATCTCTAGGACAAGTAG
CTTCGCAAACAATGGAAAGCATGTTAGCTATTAATGACGTAACTACTATGCCGATTATTCGTCCGGTAGTTTCAATGGAT
AAAAATGAAATTATTGACATAGCTAAGGATATCGACACTTATGACTTATCAATTATGCCATTCGAAGATTGTTGTACGAT
TTTTGCTCCACCATCACCTAAAACACATCCAGATCTCGAAAAGACGCGTTACTTTGAGAAGAGAATTGACGTTGAAGGAT
TATTGGAACGTTCTTTAGCAGGCGTAAAAATTACTAACATTCGTGCTGAAGAAAACTTTATGAATCAAAATGAAGAAGTT
TTTGCTGAATTATTGTAA

Upstream 100 bases:

>100_bases
ATAGTGCTAATTCTTTGGAAGAAGCTGATGAATTTAATCGAATTTTTGACCAATTATATCAAGACTTTTCAAAAATAAAT
AAAAATAGCTAGGAGTTTAT

Downstream 100 bases:

>100_bases
AGGATTTGTATATTGATTGAAATCAGTGAGTGTTTGAGATAAGGTTAACGTAATTTAAATAAAGGAGCGTACAATTATGG
AAGTATTACGCGGTGGAGAA

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MKYTEIMVRYGELSTKGKNRKDFIARLGGNIRKSLKDFEDVEIHPNRDRTHVTLNGTDSDAVINRLKKVFGIQNFSPMLK
VEKTMEAVQAGALEMMKEQLKPGMTFKINTRRSDKEFAINTDTMNRELGGFILDNFPDNDVKMKNPDITLRVEIRSNGIF
LTSEVINGAGGLPVGTAGKGMMMLSGGIDSPVAGYLGMKRGVEMEMVHFFSPPYTSEQALAKAKELSGKLAAYSGSVQFI
QVPFTEIQETIKEKCPEGYLMTIQRRMMLRLVVALAKQRGGLAIFNGESLGQVASQTMESMLAINDVTTMPIIRPVVSMD
KNEIIDIAKDIDTYDLSIMPFEDCCTIFAPPSPKTHPDLEKTRYFEKRIDVEGLLERSLAGVKITNIRAEENFMNQNEEV
FAELL

Sequences:

>Translated_405_residues
MKYTEIMVRYGELSTKGKNRKDFIARLGGNIRKSLKDFEDVEIHPNRDRTHVTLNGTDSDAVINRLKKVFGIQNFSPMLK
VEKTMEAVQAGALEMMKEQLKPGMTFKINTRRSDKEFAINTDTMNRELGGFILDNFPDNDVKMKNPDITLRVEIRSNGIF
LTSEVINGAGGLPVGTAGKGMMMLSGGIDSPVAGYLGMKRGVEMEMVHFFSPPYTSEQALAKAKELSGKLAAYSGSVQFI
QVPFTEIQETIKEKCPEGYLMTIQRRMMLRLVVALAKQRGGLAIFNGESLGQVASQTMESMLAINDVTTMPIIRPVVSMD
KNEIIDIAKDIDTYDLSIMPFEDCCTIFAPPSPKTHPDLEKTRYFEKRIDVEGLLERSLAGVKITNIRAEENFMNQNEEV
FAELL
>Mature_405_residues
MKYTEIMVRYGELSTKGKNRKDFIARLGGNIRKSLKDFEDVEIHPNRDRTHVTLNGTDSDAVINRLKKVFGIQNFSPMLK
VEKTMEAVQAGALEMMKEQLKPGMTFKINTRRSDKEFAINTDTMNRELGGFILDNFPDNDVKMKNPDITLRVEIRSNGIF
LTSEVINGAGGLPVGTAGKGMMMLSGGIDSPVAGYLGMKRGVEMEMVHFFSPPYTSEQALAKAKELSGKLAAYSGSVQFI
QVPFTEIQETIKEKCPEGYLMTIQRRMMLRLVVALAKQRGGLAIFNGESLGQVASQTMESMLAINDVTTMPIIRPVVSMD
KNEIIDIAKDIDTYDLSIMPFEDCCTIFAPPSPKTHPDLEKTRYFEKRIDVEGLLERSLAGVKITNIRAEENFMNQNEEV
FAELL

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=363, Percent_Identity=29.7520661157025, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_PEDPA (Q03EN8)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804776.1
- ProteinModelPortal:   Q03EN8
- SMR:   Q03EN8
- STRING:   Q03EN8
- GeneID:   4417067
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1293
- NMPDR:   fig|278197.10.peg.1135
- eggNOG:   COG0301
- HOGENOM:   HBG646072
- OMA:   ESIGQVA
- ProtClustDB:   PRK01565
- BioCyc:   PPEN278197:PEPE_1293-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 45321; Mature: 45321

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS51165 THUMP

Important sites: BINDING 265-265 BINDING 287-287 BINDING 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYTEIMVRYGELSTKGKNRKDFIARLGGNIRKSLKDFEDVEIHPNRDRTHVTLNGTDSD
CCHHHEEEEECCCCCCCCCHHHHHHHHCCHHHHHHHCCCCEEECCCCCCEEEEECCCCHH
AVINRLKKVFGIQNFSPMLKVEKTMEAVQAGALEMMKEQLKPGMTFKINTRRSDKEFAIN
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEE
TDTMNRELGGFILDNFPDNDVKMKNPDITLRVEIRSNGIFLTSEVINGAGGLPVGTAGKG
CCHHCHHHCCEEECCCCCCCCEECCCCEEEEEEEECCCEEEEHHHHCCCCCCCCCCCCCC
MMMLSGGIDSPVAGYLGMKRGVEMEMVHFFSPPYTSEQALAKAKELSGKLAAYSGSVQFI
EEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEECCCEEEE
QVPFTEIQETIKEKCPEGYLMTIQRRMMLRLVVALAKQRGGLAIFNGESLGQVASQTMES
ECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
MLAINDVTTMPIIRPVVSMDKNEIIDIAKDIDTYDLSIMPFEDCCTIFAPPSPKTHPDLE
HHHHCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEHHHHHEEECCCCCCCCCCHH
KTRYFEKRIDVEGLLERSLAGVKITNIRAEENFMNQNEEVFAELL
HHHHHHHHCCHHHHHHHHHCCEEEEEEECHHHHCCCCHHHHHHCC
>Mature Secondary Structure
MKYTEIMVRYGELSTKGKNRKDFIARLGGNIRKSLKDFEDVEIHPNRDRTHVTLNGTDSD
CCHHHEEEEECCCCCCCCCHHHHHHHHCCHHHHHHHCCCCEEECCCCCCEEEEECCCCHH
AVINRLKKVFGIQNFSPMLKVEKTMEAVQAGALEMMKEQLKPGMTFKINTRRSDKEFAIN
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEE
TDTMNRELGGFILDNFPDNDVKMKNPDITLRVEIRSNGIFLTSEVINGAGGLPVGTAGKG
CCHHCHHHCCEEECCCCCCCCEECCCCEEEEEEEECCCEEEEHHHHCCCCCCCCCCCCCC
MMMLSGGIDSPVAGYLGMKRGVEMEMVHFFSPPYTSEQALAKAKELSGKLAAYSGSVQFI
EEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEECCCEEEE
QVPFTEIQETIKEKCPEGYLMTIQRRMMLRLVVALAKQRGGLAIFNGESLGQVASQTMES
ECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
MLAINDVTTMPIIRPVVSMDKNEIIDIAKDIDTYDLSIMPFEDCCTIFAPPSPKTHPDLE
HHHHCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEHHHHHEEECCCCCCCCCCHH
KTRYFEKRIDVEGLLERSLAGVKITNIRAEENFMNQNEEVFAELL
HHHHHHHHCCHHHHHHHHHCCEEEEEEECHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA