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The map label for this gene is yibK [C]

Identifier: 116492956

GI number: 116492956

Start: 1196558

End: 1197076

Strand: Reverse

Name: yibK [C]

Synonym: PEPE_1195

Alternate gene names: 116492956

Gene position: 1197076-1196558 (Counterclockwise)

Preceding gene: 116492957

Following gene: 116492955

Centisome position: 65.33

GC content: 34.1

Gene sequence:

>519_bases
ATGACAAATCACATTGTATTATTTGAACCACTAATGCCGGCTAATACTGGTAATATTGCTCGTACTTGTGCGGGTACAAA
TACAGTTTTGCACTTAATTAAACCTTTGGGATTTACCATCAATGATAAGACGGTAAAGCGTGCAGGACTGGATTATTGGG
ACAAAGTTGAAATTGTATATCATGAAAATTTACCCGAATTTTTAGCAACGGTTCCTGATAAAAAAAGATTATTTGTTGTT
TCAAAATTTGCTAAACGTGCATACTCACAGGCAGATTATACCAGCGAAAATAATGATGAAGATAATTATTTATTATTTGG
AAAAGAAACTACAGGGCTACCCGAAGCATTTATGCGGCAATATGAAGAATGTGCAATACGGATTCCACAAAATGATGAAA
ACATTCGGGCACTAAATTTGTCGAATTCAGCGGCGATTGTAATTTATGAAGTGTTACGTCAACAAGGATTCCCTAATTTA
GAGATGAGCCATTTGTATGAAAATGATAAATTAAAATAG

Upstream 100 bases:

>100_bases
TTCGTTTGAAACGGGCAGATTGACTTCACGAACGTAGATACTCGCCGCGTAATACCGAGTATCTGCGTTTTTAATTCTAA
AAAATATTTGGAGAAAAATT

Downstream 100 bases:

>100_bases
GATTTGATATGGCAAAAACAAGAAAAAAACGAAAAACAAAGACAAGACGTAAAACTAAGCAAAAGACCCAAGTACCAACA
AAAAATCTTTTAGGTTTAAC

Product: rRNA methylase (SpoU class)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 172; Mature: 171

Protein sequence:

>172_residues
MTNHIVLFEPLMPANTGNIARTCAGTNTVLHLIKPLGFTINDKTVKRAGLDYWDKVEIVYHENLPEFLATVPDKKRLFVV
SKFAKRAYSQADYTSENNDEDNYLLFGKETTGLPEAFMRQYEECAIRIPQNDENIRALNLSNSAAIVIYEVLRQQGFPNL
EMSHLYENDKLK

Sequences:

>Translated_172_residues
MTNHIVLFEPLMPANTGNIARTCAGTNTVLHLIKPLGFTINDKTVKRAGLDYWDKVEIVYHENLPEFLATVPDKKRLFVV
SKFAKRAYSQADYTSENNDEDNYLLFGKETTGLPEAFMRQYEECAIRIPQNDENIRALNLSNSAAIVIYEVLRQQGFPNL
EMSHLYENDKLK
>Mature_171_residues
TNHIVLFEPLMPANTGNIARTCAGTNTVLHLIKPLGFTINDKTVKRAGLDYWDKVEIVYHENLPEFLATVPDKKRLFVVS
KFAKRAYSQADYTSENNDEDNYLLFGKETTGLPEAFMRQYEECAIRIPQNDENIRALNLSNSAAIVIYEVLRQQGFPNLE
MSHLYENDKLK

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=156, Percent_Identity=41.025641025641, Blast_Score=119, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 19717; Mature: 19585

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNHIVLFEPLMPANTGNIARTCAGTNTVLHLIKPLGFTINDKTVKRAGLDYWDKVEIVY
CCCCEEEECCCCCCCCCCEEEECCCCHHHHHHHHHCCCEECCHHHHHCCCCCCCEEEEEE
HENLPEFLATVPDKKRLFVVSKFAKRAYSQADYTSENNDEDNYLLFGKETTGLPEAFMRQ
ECCCHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHH
YEECAIRIPQNDENIRALNLSNSAAIVIYEVLRQQGFPNLEMSHLYENDKLK
HHHHEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
TNHIVLFEPLMPANTGNIARTCAGTNTVLHLIKPLGFTINDKTVKRAGLDYWDKVEIVY
CCCEEEECCCCCCCCCCEEEECCCCHHHHHHHHHCCCEECCHHHHHCCCCCCCEEEEEE
HENLPEFLATVPDKKRLFVVSKFAKRAYSQADYTSENNDEDNYLLFGKETTGLPEAFMRQ
ECCCHHHHHCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHH
YEECAIRIPQNDENIRALNLSNSAAIVIYEVLRQQGFPNLEMSHLYENDKLK
HHHHEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA