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The map label for this gene is def

Identifier: 116492920

GI number: 116492920

Start: 1157694

End: 1158248

Strand: Direct

Name: def

Synonym: PEPE_1159

Alternate gene names: 116492920

Gene position: 1157694-1158248 (Clockwise)

Preceding gene: 116492908

Following gene: 116492922

Centisome position: 63.18

GC content: 39.1

Gene sequence:

>555_bases
TTGATTAAAATGAACGATATCACTCGTGACGGAAATCCCGTTTTACGAAAACGTGCTGAAAAGCTTACTTTTCCGCTCGA
TCCTAAATATCTCAAAGTTGCCGATGAAATGATGGAATATTTAAAAAATAGCCAAGATCCAGCCATTGCTGAAAAATACC
ACTTAAGAGCCGGCGTTGGTTTGGCCGCTCCACAGATTGGTTTATCAATCCAAATGGCTTCAGTCCTTGTTCCTGGGCCA
GATAATACCATCGATTTAGAAGAAACATTAGTTAATCCTGTAATCGTTAGTCAATCGGTTCAAATTGCTGCTTTGGAGGA
AGGCGAAGGCTGTCTTTCGGTCGATAAAGACGTTCCTGGATATGTTCCTCGTCACGACCGCATCACTGTGCGTTACCAAA
CCTTGGATGGCGAAGAAAAAACTATTAAACTCCGCGATTATCCTGCAATTGTTTGTCAACATGAAATTGATCATCTAAAA
GGCACTTTATTCTATGATCATATCAATAAAGAAAATCCTTTAGATATTGAAGATAATGCTATTTTAATCGGCTAA

Upstream 100 bases:

>100_bases
TTTCTTTTATACTCATTTTAATATTCCTTTTAAGATTTATTTTGTGTTAATACTATATTATATATTATACTTGTTGTTAG
ACGAATTGGAGGTTTTTATC

Downstream 100 bases:

>100_bases
TTATAAAAAAGAAGCTATGCCTGCATGGTGAATCTCAAAAGTTAAGTTATCTACTTAATTTCTGACTATTCATTCCTTTG
TGGCATAGCTTCTTTTTTAT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MIKMNDITRDGNPVLRKRAEKLTFPLDPKYLKVADEMMEYLKNSQDPAIAEKYHLRAGVGLAAPQIGLSIQMASVLVPGP
DNTIDLEETLVNPVIVSQSVQIAALEEGEGCLSVDKDVPGYVPRHDRITVRYQTLDGEEKTIKLRDYPAIVCQHEIDHLK
GTLFYDHINKENPLDIEDNAILIG

Sequences:

>Translated_184_residues
MIKMNDITRDGNPVLRKRAEKLTFPLDPKYLKVADEMMEYLKNSQDPAIAEKYHLRAGVGLAAPQIGLSIQMASVLVPGP
DNTIDLEETLVNPVIVSQSVQIAALEEGEGCLSVDKDVPGYVPRHDRITVRYQTLDGEEKTIKLRDYPAIVCQHEIDHLK
GTLFYDHINKENPLDIEDNAILIG
>Mature_184_residues
MIKMNDITRDGNPVLRKRAEKLTFPLDPKYLKVADEMMEYLKNSQDPAIAEKYHLRAGVGLAAPQIGLSIQMASVLVPGP
DNTIDLEETLVNPVIVSQSVQIAALEEGEGCLSVDKDVPGYVPRHDRITVRYQTLDGEEKTIKLRDYPAIVCQHEIDHLK
GTLFYDHINKENPLDIEDNAILIG

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_PEDPA (Q03F09)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804655.1
- ProteinModelPortal:   Q03F09
- SMR:   Q03F09
- STRING:   Q03F09
- GeneID:   4417622
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1159
- NMPDR:   fig|278197.10.peg.1018
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   IDHLNGH
- ProtClustDB:   PRK00150
- BioCyc:   PPEN278197:PEPE_1159-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20575; Mature: 20575

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: ACT_SITE 155-155

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKMNDITRDGNPVLRKRAEKLTFPLDPKYLKVADEMMEYLKNSQDPAIAEKYHLRAGVG
CCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
LAAPQIGLSIQMASVLVPGPDNTIDLEETLVNPVIVSQSVQIAALEEGEGCLSVDKDVPG
CCCCCCCCEEEEEEEEECCCCCEEEHHHHHCCCEEECCCEEEEEEECCCCCEEECCCCCC
YVPRHDRITVRYQTLDGEEKTIKLRDYPAIVCQHEIDHLKGTLFYDHINKENPLDIEDNA
CCCCCCEEEEEEEEECCCCEEEEEECCCEEEEEHHHHHHCCEEEEEECCCCCCCCCCCCE
ILIG
EEEC
>Mature Secondary Structure
MIKMNDITRDGNPVLRKRAEKLTFPLDPKYLKVADEMMEYLKNSQDPAIAEKYHLRAGVG
CCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
LAAPQIGLSIQMASVLVPGPDNTIDLEETLVNPVIVSQSVQIAALEEGEGCLSVDKDVPG
CCCCCCCCEEEEEEEEECCCCCEEEHHHHHCCCEEECCCEEEEEEECCCCCEEECCCCCC
YVPRHDRITVRYQTLDGEEKTIKLRDYPAIVCQHEIDHLKGTLFYDHINKENPLDIEDNA
CCCCCCEEEEEEEEECCCCEEEEEECCCEEEEEHHHHHHCCEEEEEECCCCCCCCCCCCE
ILIG
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA