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The map label for this gene is tig

Identifier: 116492903

GI number: 116492903

Start: 1141197

End: 1142504

Strand: Reverse

Name: tig

Synonym: PEPE_1142

Alternate gene names: 116492903

Gene position: 1142504-1141197 (Counterclockwise)

Preceding gene: 116492904

Following gene: 116492902

Centisome position: 62.35

GC content: 38.0

Gene sequence:

>1308_bases
ATGACTGCAAAATGGGAAAAAACAGGTACTAACGAAGGTAAATTAACTTTCGAAGTAGGTACTGACAAAATTAAAGAAGG
CGTTGATAAGGCGTTCGATCGTACTAAAAAACAATTGAACGTACCTGGATTCCGTAAGGGACACGTTCCACGCCAAATCT
TCAATAAGATGTATGGTGAAGAAGCATTGTACCAAGATGCTTTGAACATCGTGTTACCAGAGGCTTATGAAGCTGCTATC
AAAGAAGCTGGTATCGAACCAGTTGATCAACCACAAGTTGATGTTGAAAGCATGGAAAAAGGCGAAGCTTGGGTCCTAAG
TGCAATTGTTACAGTTAAGCCAGAAGTTAAGCTTGGCGAATACAAAGGTGTTAAAGTTATCAAGCAAAGCACACGTGTAA
CTTCAAAAGAAGTTGACGAAGAAATTGAAAAGAAACGTGAACAACAAGCTGAATTAGTTCTTAAGGAAGGCAAACCAGCA
GAAAACGGCGATACAGTAACAATCGATTTTGATGGTTCTGTTGATGGCGTTGCTTTTGATGGCGGTAAAGCTGACAATTA
CGACCTTGTATTAGGTTCAAACTCATTCATTCCAGGATTTGAAGATCAATTGATTGGTCATAATACTGATGAAGATGTAG
ATGTTAACGTTACATTCCCTGACGACTACCAAGCTGAAGACTTACAAGGTAAGGATGCCTTGTTTAAGGTTAAGATTCAT
GAAATCAAGACTAAGGAATTACCTGAACTTGATGATGAATTCGCTAAGGATGTTGACGAAGATGTTGATTCTCTTGAAGA
TTTAAAAGTTAAGACAAAAGAAGAAATCAAATCACGTAAGATCCAAGCTGCTAGTGATGCTAAAGAGACAGAAGCTGTTC
AAGCTGCTGTTGACAATGCTGAAATTGCAGAAATTCCAGAAGCAATGTTAAATGAAGATGTTGATCGTCAAGTTAACCAA
TATCTTGCAAACATGCAACAACAAGGAATTAGCCCTGAAATGTACTTCCAATTAACTGGTTCATCAGAAGACCAATTACG
TGACCAATTACGTGAAGGTGCTGAAAACCGTGTTAAGACAACTCTTGTTTTAGAAGCAATCGTTGAAGCTGAAAAGATTG
ATCCTTCAGATGACGAAGTTGCTGCTGAAATCAAAGATTTAGCAGAACAATACGGCATGGAAGAAAAAGCTGTTCGCTCA
GCTCTTTCAGAAGATATGATCAAGCATGATGTTGCTATTAAATCAGCTGTTGAACTTATCAAAGACTCAGCAATTGAAGA
ACCAAAATCAAAGGCTGCTAAAAAATAA

Upstream 100 bases:

>100_bases
TGTTGTATTTTTATTTACTTTTCAAACCATATACGTTAAGATTATAAGCGTATGCAATCCAGAATTGTAATGAAATAAAT
CATCTGTCGGAGGGAACACA

Downstream 100 bases:

>100_bases
TTAAAACTATTTTTAACGATTTTGAGGTTTTATCTCAAAATCGTTTTTGTTTATTAACCACTAAAGTTATAGAAATTGGC
TTATAGTCGTTTATTTTGCT

Product: FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTAKWEKTGTNEGKLTFEVGTDKIKEGVDKAFDRTKKQLNVPGFRKGHVPRQIFNKMYGEEALYQDALNIVLPEAYEAAI
KEAGIEPVDQPQVDVESMEKGEAWVLSAIVTVKPEVKLGEYKGVKVIKQSTRVTSKEVDEEIEKKREQQAELVLKEGKPA
ENGDTVTIDFDGSVDGVAFDGGKADNYDLVLGSNSFIPGFEDQLIGHNTDEDVDVNVTFPDDYQAEDLQGKDALFKVKIH
EIKTKELPELDDEFAKDVDEDVDSLEDLKVKTKEEIKSRKIQAASDAKETEAVQAAVDNAEIAEIPEAMLNEDVDRQVNQ
YLANMQQQGISPEMYFQLTGSSEDQLRDQLREGAENRVKTTLVLEAIVEAEKIDPSDDEVAAEIKDLAEQYGMEEKAVRS
ALSEDMIKHDVAIKSAVELIKDSAIEEPKSKAAKK

Sequences:

>Translated_435_residues
MTAKWEKTGTNEGKLTFEVGTDKIKEGVDKAFDRTKKQLNVPGFRKGHVPRQIFNKMYGEEALYQDALNIVLPEAYEAAI
KEAGIEPVDQPQVDVESMEKGEAWVLSAIVTVKPEVKLGEYKGVKVIKQSTRVTSKEVDEEIEKKREQQAELVLKEGKPA
ENGDTVTIDFDGSVDGVAFDGGKADNYDLVLGSNSFIPGFEDQLIGHNTDEDVDVNVTFPDDYQAEDLQGKDALFKVKIH
EIKTKELPELDDEFAKDVDEDVDSLEDLKVKTKEEIKSRKIQAASDAKETEAVQAAVDNAEIAEIPEAMLNEDVDRQVNQ
YLANMQQQGISPEMYFQLTGSSEDQLRDQLREGAENRVKTTLVLEAIVEAEKIDPSDDEVAAEIKDLAEQYGMEEKAVRS
ALSEDMIKHDVAIKSAVELIKDSAIEEPKSKAAKK
>Mature_434_residues
TAKWEKTGTNEGKLTFEVGTDKIKEGVDKAFDRTKKQLNVPGFRKGHVPRQIFNKMYGEEALYQDALNIVLPEAYEAAIK
EAGIEPVDQPQVDVESMEKGEAWVLSAIVTVKPEVKLGEYKGVKVIKQSTRVTSKEVDEEIEKKREQQAELVLKEGKPAE
NGDTVTIDFDGSVDGVAFDGGKADNYDLVLGSNSFIPGFEDQLIGHNTDEDVDVNVTFPDDYQAEDLQGKDALFKVKIHE
IKTKELPELDDEFAKDVDEDVDSLEDLKVKTKEEIKSRKIQAASDAKETEAVQAAVDNAEIAEIPEAMLNEDVDRQVNQY
LANMQQQGISPEMYFQLTGSSEDQLRDQLREGAENRVKTTLVLEAIVEAEKIDPSDDEVAAEIKDLAEQYGMEEKAVRSA
LSEDMIKHDVAIKSAVELIKDSAIEEPKSKAAKK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=381, Percent_Identity=30.4461942257218, Blast_Score=173, Evalue=2e-44,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_PEDPA (Q03F26)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804638.1
- ProteinModelPortal:   Q03F26
- STRING:   Q03F26
- GeneID:   4417824
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1142
- NMPDR:   fig|278197.10.peg.1003
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   Q03F26
- BioCyc:   PPEN278197:PEPE_1142-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 48386; Mature: 48255

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKWEKTGTNEGKLTFEVGTDKIKEGVDKAFDRTKKQLNVPGFRKGHVPRQIFNKMYGE
CCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCH
EALYQDALNIVLPEAYEAAIKEAGIEPVDQPQVDVESMEKGEAWVLSAIVTVKPEVKLGE
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEEEHHEECCCCCCCC
YKGVKVIKQSTRVTSKEVDEEIEKKREQQAELVLKEGKPAENGDTVTIDFDGSVDGVAFD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEC
GGKADNYDLVLGSNSFIPGFEDQLIGHNTDEDVDVNVTFPDDYQAEDLQGKDALFKVKIH
CCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEE
EIKTKELPELDDEFAKDVDEDVDSLEDLKVKTKEEIKSRKIQAASDAKETEAVQAAVDNA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
EIAEIPEAMLNEDVDRQVNQYLANMQQQGISPEMYFQLTGSSEDQLRDQLREGAENRVKT
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
TLVLEAIVEAEKIDPSDDEVAAEIKDLAEQYGMEEKAVRSALSEDMIKHDVAIKSAVELI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDSAIEEPKSKAAKK
HHHHCCCHHHHCCCC
>Mature Secondary Structure 
TAKWEKTGTNEGKLTFEVGTDKIKEGVDKAFDRTKKQLNVPGFRKGHVPRQIFNKMYGE
CCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCH
EALYQDALNIVLPEAYEAAIKEAGIEPVDQPQVDVESMEKGEAWVLSAIVTVKPEVKLGE
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEEEHHEECCCCCCCC
YKGVKVIKQSTRVTSKEVDEEIEKKREQQAELVLKEGKPAENGDTVTIDFDGSVDGVAFD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEC
GGKADNYDLVLGSNSFIPGFEDQLIGHNTDEDVDVNVTFPDDYQAEDLQGKDALFKVKIH
CCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEE
EIKTKELPELDDEFAKDVDEDVDSLEDLKVKTKEEIKSRKIQAASDAKETEAVQAAVDNA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
EIAEIPEAMLNEDVDRQVNQYLANMQQQGISPEMYFQLTGSSEDQLRDQLREGAENRVKT
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
TLVLEAIVEAEKIDPSDDEVAAEIKDLAEQYGMEEKAVRSALSEDMIKHDVAIKSAVELI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDSAIEEPKSKAAKK
HHHHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA