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The map label for this gene is tuf [H]

Identifier: 116492904

GI number: 116492904

Start: 1142645

End: 1143832

Strand: Reverse

Name: tuf [H]

Synonym: PEPE_1143

Alternate gene names: 116492904

Gene position: 1143832-1142645 (Counterclockwise)

Preceding gene: 116492905

Following gene: 116492903

Centisome position: 62.42

GC content: 41.16

Gene sequence:

>1188_bases
ATGGCAAAAGAACATTACGAAAGAACAAAACCCCATGTAAATATTGGTACAATTGGCCACGTTGACCATGGGAAAACTAC
TTTGACAGCTGCTATTACAAAAGTGTTGTCAGAAAAAGGACTAGCTAAAGCTTCAGATTACGCTGATATTGATGCTGCTC
CAGAAGAAAAGGAACGTGGTATCACAATCAACACAGCCCACGTTGAATATGAAACAGAAAAGCGTCATTACGCTCATATC
GATGCTCCAGGACATGCTGATTACGTTAAGAACATGATCACTGGTGCTGCACAAATGGATGGTGCTATCTTAGTTGTTGC
TGCAACTGATGGTCCTATGCCACAAACACGTGAACATATCCTTCTTGCTCACCAAGTTGGTGTTGACTACATCGTTGTCT
TCTTAAACAAGACTGATCTTGTTGATGATGATGAATTAGTTGACCTTGTTGAGATGGAAGTTCGTGAACTTCTATCTGAA
TATGATTTCCCTGGTGACGATGTTCCTGTACTTCGCGGTTCAGCTTTGAAAGCTCTTGAAGGCGATGCAGAACAAGAAAA
AGTTATCATGGATCTTATGGATACAATCGACGAATACATTCCAACACCAGAACGTTCTACAGACAAGCCATTCTTGATGC
CTGTTGAAGACGTATTTACAATTACTGGTCGTGGTACTGTTGCTTCTGGTCGTATCGATCGTGGTGAAATCAAAGTCGGT
GACGAAGTTGAAATCGTTGGTCTTAAAGAAGACGTAACAAAGACAACTGTTACAGGAATTGAAATGTTCCGTAAGACTCT
TGATGTTGGTGAAGCTGGTGATAACATTGGTGCACTTCTACGTGGTGTTAACCGTGAAGATGTTGTCCGTGGACAAGTTT
TGGCTGCTCCTGGCTCAATCCAAACACACAAGAAGTTCAAGGGTGAAGTTTATATCCTTTCTAAGGATGAAGGTGGACGT
CATACTCCATTCTTCTCAAACTACCGTCCTCAATTCTACTTCCATACAACTGATGTAACAGGTGTTATTGAATTACCTGA
CAACGTTGAAATGGTTATGCCTGGTGATAACGTTACATTTACTGTAGAACTTATCGAACCAGTTGCTATTGAAAAAGGTA
CTAAGTTCACTGTTCGTGAAGGTGGACACACTGTTGGTGCTGGTGTTGTATCAGAAATTGACGACTAA

Upstream 100 bases:

>100_bases
TTTGCCTTTCGTGGAGGATGTAGTATAATATTTTTTAAAGGATCTAGTTATTCATTTTGAATGCTGAGTGATTCTTACGA
CATACAGGAGGTTCGTATTA

Downstream 100 bases:

>100_bases
GCTTAATTGCTTAATAAAAACCCGCTTTTGCGGGTTTTTTTGTTGTATTTTTATTTACTTTTCAAACCATATACGTTAAG
ATTATAAGCGTATGCAATCC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MAKEHYERTKPHVNIGTIGHVDHGKTTLTAAITKVLSEKGLAKASDYADIDAAPEEKERGITINTAHVEYETEKRHYAHI
DAPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLAHQVGVDYIVVFLNKTDLVDDDELVDLVEMEVRELLSE
YDFPGDDVPVLRGSALKALEGDAEQEKVIMDLMDTIDEYIPTPERSTDKPFLMPVEDVFTITGRGTVASGRIDRGEIKVG
DEVEIVGLKEDVTKTTVTGIEMFRKTLDVGEAGDNIGALLRGVNREDVVRGQVLAAPGSIQTHKKFKGEVYILSKDEGGR
HTPFFSNYRPQFYFHTTDVTGVIELPDNVEMVMPGDNVTFTVELIEPVAIEKGTKFTVREGGHTVGAGVVSEIDD

Sequences:

>Translated_395_residues
MAKEHYERTKPHVNIGTIGHVDHGKTTLTAAITKVLSEKGLAKASDYADIDAAPEEKERGITINTAHVEYETEKRHYAHI
DAPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLAHQVGVDYIVVFLNKTDLVDDDELVDLVEMEVRELLSE
YDFPGDDVPVLRGSALKALEGDAEQEKVIMDLMDTIDEYIPTPERSTDKPFLMPVEDVFTITGRGTVASGRIDRGEIKVG
DEVEIVGLKEDVTKTTVTGIEMFRKTLDVGEAGDNIGALLRGVNREDVVRGQVLAAPGSIQTHKKFKGEVYILSKDEGGR
HTPFFSNYRPQFYFHTTDVTGVIELPDNVEMVMPGDNVTFTVELIEPVAIEKGTKFTVREGGHTVGAGVVSEIDD
>Mature_394_residues
AKEHYERTKPHVNIGTIGHVDHGKTTLTAAITKVLSEKGLAKASDYADIDAAPEEKERGITINTAHVEYETEKRHYAHID
APGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLAHQVGVDYIVVFLNKTDLVDDDELVDLVEMEVRELLSEY
DFPGDDVPVLRGSALKALEGDAEQEKVIMDLMDTIDEYIPTPERSTDKPFLMPVEDVFTITGRGTVASGRIDRGEIKVGD
EVEIVGLKEDVTKTTVTGIEMFRKTLDVGEAGDNIGALLRGVNREDVVRGQVLAAPGSIQTHKKFKGEVYILSKDEGGRH
TPFFSNYRPQFYFHTTDVTGVIELPDNVEMVMPGDNVTFTVELIEPVAIEKGTKFTVREGGHTVGAGVVSEIDD

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=392, Percent_Identity=58.1632653061224, Blast_Score=476, Evalue=1e-134,
Organism=Homo sapiens, GI4503475, Length=453, Percent_Identity=30.6843267108168, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI223555963, Length=442, Percent_Identity=29.6380090497738, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI5729864, Length=436, Percent_Identity=29.8165137614679, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI4503471, Length=453, Percent_Identity=30.4635761589404, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI194018522, Length=373, Percent_Identity=27.8820375335121, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI194097354, Length=373, Percent_Identity=27.8820375335121, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI194018520, Length=373, Percent_Identity=27.8820375335121, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI46094014, Length=374, Percent_Identity=28.0748663101604, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI54607086, Length=321, Percent_Identity=29.595015576324, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI4503507, Length=267, Percent_Identity=27.7153558052434, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI157426893, Length=241, Percent_Identity=28.2157676348548, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=33.8345864661654, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=72.0101781170484, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=72.0101781170484, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI2367247, Length=334, Percent_Identity=31.437125748503, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI48994988, Length=276, Percent_Identity=32.9710144927536, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789108, Length=329, Percent_Identity=31.0030395136778, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788922, Length=331, Percent_Identity=25.9818731117825, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=397, Percent_Identity=52.3929471032746, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25141371, Length=388, Percent_Identity=46.9072164948454, Blast_Score=348, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI17552884, Length=456, Percent_Identity=32.2368421052632, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=456, Percent_Identity=32.2368421052632, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI32566303, Length=437, Percent_Identity=30.6636155606407, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI115532067, Length=432, Percent_Identity=28.4722222222222, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=432, Percent_Identity=28.4722222222222, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=439, Percent_Identity=26.879271070615, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=272, Percent_Identity=28.3088235294118, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32566301, Length=164, Percent_Identity=40.8536585365854, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17557151, Length=302, Percent_Identity=28.8079470198675, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=288, Percent_Identity=26.3888888888889, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=394, Percent_Identity=63.9593908629442, Blast_Score=524, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325337, Length=451, Percent_Identity=30.1552106430155, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=451, Percent_Identity=30.1552106430155, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6320377, Length=343, Percent_Identity=30.9037900874636, Blast_Score=102, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6322937, Length=329, Percent_Identity=26.1398176291793, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=270, Percent_Identity=27.7777777777778, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=246, Percent_Identity=30.4878048780488, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=203, Percent_Identity=29.064039408867, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281363316, Length=391, Percent_Identity=58.5677749360614, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI17864358, Length=391, Percent_Identity=58.5677749360614, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI19921738, Length=387, Percent_Identity=50.9043927648579, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45553807, Length=454, Percent_Identity=29.5154185022026, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI45553816, Length=454, Percent_Identity=29.5154185022026, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24651721, Length=454, Percent_Identity=29.5154185022026, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17864154, Length=454, Percent_Identity=29.5154185022026, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24652838, Length=454, Percent_Identity=29.5154185022026, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI17137572, Length=454, Percent_Identity=29.5154185022026, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI45550900, Length=448, Percent_Identity=29.0178571428571, Blast_Score=134, Evalue=8e-32,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=30.6930693069307, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17137380, Length=345, Percent_Identity=30.4347826086957, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24647048, Length=357, Percent_Identity=25.7703081232493, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24647046, Length=357, Percent_Identity=25.7703081232493, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43312; Mature: 43181

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEHYERTKPHVNIGTIGHVDHGKTTLTAAITKVLSEKGLAKASDYADIDAAPEEKERG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCC
ITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLAHQVGVDYIVVFLNKTDLVDDDELVDLVEMEVRELLSEYDFPGDDVPVLRGSALKALE
EEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHC
GDAEQEKVIMDLMDTIDEYIPTPERSTDKPFLMPVEDVFTITGRGTVASGRIDRGEIKVG
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHEEEEECCCEEECCCCCCCCEEEC
DEVEIVGLKEDVTKTTVTGIEMFRKTLDVGEAGDNIGALLRGVNREDVVRGQVLAAPGSI
CEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHCCEEEECCCCC
QTHKKFKGEVYILSKDEGGRHTPFFSNYRPQFYFHTTDVTGVIELPDNVEMVMPGDNVTF
CCHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCEEE
TVELIEPVAIEKGTKFTVREGGHTVGAGVVSEIDD
EEEEECCEEECCCCEEEEECCCCEECCCHHHHCCC
>Mature Secondary Structure 
AKEHYERTKPHVNIGTIGHVDHGKTTLTAAITKVLSEKGLAKASDYADIDAAPEEKERG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCC
ITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHHE
LLAHQVGVDYIVVFLNKTDLVDDDELVDLVEMEVRELLSEYDFPGDDVPVLRGSALKALE
EEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHC
GDAEQEKVIMDLMDTIDEYIPTPERSTDKPFLMPVEDVFTITGRGTVASGRIDRGEIKVG
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHEEEEECCCEEECCCCCCCCEEEC
DEVEIVGLKEDVTKTTVTGIEMFRKTLDVGEAGDNIGALLRGVNREDVVRGQVLAAPGSI
CEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHCCEEEECCCCC
QTHKKFKGEVYILSKDEGGRHTPFFSNYRPQFYFHTTDVTGVIELPDNVEMVMPGDNVTF
CCHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCEEE
TVELIEPVAIEKGTKFTVREGGHTVGAGVVSEIDD
EEEEECCEEECCCCEEEEECCCCEECCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA