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The map label for this gene is carA [H]

Identifier: 116492707

GI number: 116492707

Start: 943478

End: 944557

Strand: Direct

Name: carA [H]

Synonym: PEPE_0943

Alternate gene names: 116492707

Gene position: 943478-944557 (Clockwise)

Preceding gene: 116492706

Following gene: 116492708

Centisome position: 51.49

GC content: 40.28

Gene sequence:

>1080_bases
ATGGCAGACCGATATTTAATATTAGAAGACGGTTCCGTTTTTAAGGGTAAAGCATTCGGATCGCGAGCAACCACCTTTGG
CGAAGTTGTTTTTACTACCGTAATGACTGGATATCAACAAGTCATTACCAATCAAATTTATCATAATCAGATTATTGTTT
TCTCTGAACCCACAATTGGTGCTGTAGGAATTGATCGGGCGAGCTATGAATCGGTTGATCCCACTTGTAAAGGCGTCATT
GTTAGAGATGTAGCTGCGGTTTCCATTAATCGACGTCGCCGCTTATCTTTAGATGAATACTTAAAACGGCAAAACATTCC
AGGAATTTCTGGAATTGACACCCGTGCTCTGATTAAAAAATTACGTTCAACCGGAACCATTAAAGGCAGTATTATAGATC
CTGTTAAGGATTTGGAACATGCTTTCGATCAGCTAAGAGCTATTGTTTTAACTAATCAACAGGTTAATCAAGTATCTACT
CCCAAAGCCTTTCCAAATCCAGGAAATGGCAAAAATGTGGTAGTCGTTGATTTAGGGCTTAAATACGGAATTCTTCGGCA
ACTCAGCCAGCGGGATTGTAATGTAACAGTTCTGGGGTATTCAGCTACTGCAGATAGTATTTTTAGTTTAGATCCTGATG
GTGTTATTATTTCGACCGGTCCGGGGAATCCGCGCGATTTGCCCCCAGAAGTGATTGAGATGATTCAGATTGTTGAGCGT
AATATCCCGTTATTTGCGATTGGATTGGGACATGAAGCTTTTGCAATTGCGAATGGCTGTGAAATTATGGAATTATCATC
TGAACACCATGGTTCTAATCATCCAATTCTATCTGTTATCACAGATGAAATTATGTACGCCTCACATGGACAAGGATTTA
CGGTAGACCCTAATTCTGTAGATCGTAATGATTTAATCGTAACGCATTACGATTTAATTGATCACTCCGTGCAAGGACTA
CGTCACCGAGATTTTCCAGCTTTCAGTGTTCAATTCTTTCCAGATAGTTCTCCTGGTCCAACGGAAGCTACCGAATTGTT
TGATGAATTTATGGAAATGATGACAAGAGGGGGTCGTTGA

Upstream 100 bases:

>100_bases
AGCACAAAACGAGAAAATTAACGTTTTTGTCCGCGAAATTGATGATGAAGACGTTGTTCTCTTAGAAAATTAACATGATT
TAACTGTGGAGGTGGCTATT

Downstream 100 bases:

>100_bases
TTTGGCAAGATCGCGTGCTAAAAATATTTTGATTATTGGAGCAGGGCCAACACAAATCGGACGTGATACCGAAAGTGAAG
CGGCCGCCGTTCAAATAATC

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MADRYLILEDGSVFKGKAFGSRATTFGEVVFTTVMTGYQQVITNQIYHNQIIVFSEPTIGAVGIDRASYESVDPTCKGVI
VRDVAAVSINRRRRLSLDEYLKRQNIPGISGIDTRALIKKLRSTGTIKGSIIDPVKDLEHAFDQLRAIVLTNQQVNQVST
PKAFPNPGNGKNVVVVDLGLKYGILRQLSQRDCNVTVLGYSATADSIFSLDPDGVIISTGPGNPRDLPPEVIEMIQIVER
NIPLFAIGLGHEAFAIANGCEIMELSSEHHGSNHPILSVITDEIMYASHGQGFTVDPNSVDRNDLIVTHYDLIDHSVQGL
RHRDFPAFSVQFFPDSSPGPTEATELFDEFMEMMTRGGR

Sequences:

>Translated_359_residues
MADRYLILEDGSVFKGKAFGSRATTFGEVVFTTVMTGYQQVITNQIYHNQIIVFSEPTIGAVGIDRASYESVDPTCKGVI
VRDVAAVSINRRRRLSLDEYLKRQNIPGISGIDTRALIKKLRSTGTIKGSIIDPVKDLEHAFDQLRAIVLTNQQVNQVST
PKAFPNPGNGKNVVVVDLGLKYGILRQLSQRDCNVTVLGYSATADSIFSLDPDGVIISTGPGNPRDLPPEVIEMIQIVER
NIPLFAIGLGHEAFAIANGCEIMELSSEHHGSNHPILSVITDEIMYASHGQGFTVDPNSVDRNDLIVTHYDLIDHSVQGL
RHRDFPAFSVQFFPDSSPGPTEATELFDEFMEMMTRGGR
>Mature_358_residues
ADRYLILEDGSVFKGKAFGSRATTFGEVVFTTVMTGYQQVITNQIYHNQIIVFSEPTIGAVGIDRASYESVDPTCKGVIV
RDVAAVSINRRRRLSLDEYLKRQNIPGISGIDTRALIKKLRSTGTIKGSIIDPVKDLEHAFDQLRAIVLTNQQVNQVSTP
KAFPNPGNGKNVVVVDLGLKYGILRQLSQRDCNVTVLGYSATADSIFSLDPDGVIISTGPGNPRDLPPEVIEMIQIVERN
IPLFAIGLGHEAFAIANGCEIMELSSEHHGSNHPILSVITDEIMYASHGQGFTVDPNSVDRNDLIVTHYDLIDHSVQGLR
HRDFPAFSVQFFPDSSPGPTEATELFDEFMEMMTRGGR

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=362, Percent_Identity=33.7016574585635, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI169790915, Length=370, Percent_Identity=30.5405405405405, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI21361331, Length=370, Percent_Identity=30.5405405405405, Blast_Score=162, Evalue=5e-40,
Organism=Escherichia coli, GI1786215, Length=373, Percent_Identity=36.7292225201072, Blast_Score=243, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI193204318, Length=360, Percent_Identity=31.3888888888889, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6324878, Length=374, Percent_Identity=31.283422459893, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6322331, Length=395, Percent_Identity=29.873417721519, Blast_Score=158, Evalue=1e-39,
Organism=Drosophila melanogaster, GI45555749, Length=377, Percent_Identity=34.7480106100796, Blast_Score=181, Evalue=7e-46,
Organism=Drosophila melanogaster, GI24642586, Length=377, Percent_Identity=34.7480106100796, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39461; Mature: 39330

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRYLILEDGSVFKGKAFGSRATTFGEVVFTTVMTGYQQVITNQIYHNQIIVFSEPTIG
CCCCEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCC
AVGIDRASYESVDPTCKGVIVRDVAAVSINRRRRLSLDEYLKRQNIPGISGIDTRALIKK
EEECCCCCCCCCCCCCCCEEEHHHHHHHCCCHHCCCHHHHHHHCCCCCCCCCCHHHHHHH
LRSTGTIKGSIIDPVKDLEHAFDQLRAIVLTNQQVNQVSTPKAFPNPGNGKNVVVVDLGL
HHHCCCCCCCCCCHHHHHHHHHHHHHEEEECCCHHCCCCCCCCCCCCCCCCEEEEEECCC
KYGILRQLSQRDCNVTVLGYSATADSIFSLDPDGVIISTGPGNPRDLPPEVIEMIQIVER
HHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHC
NIPLFAIGLGHEAFAIANGCEIMELSSEHHGSNHPILSVITDEIMYASHGQGFTVDPNSV
CCCEEEEECCCCEEEEECCCEEEEECCCCCCCCCCEEEEEHHHHHEECCCCCEEECCCCC
DRNDLIVTHYDLIDHSVQGLRHRDFPAFSVQFFPDSSPGPTEATELFDEFMEMMTRGGR
CCCCEEEEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ADRYLILEDGSVFKGKAFGSRATTFGEVVFTTVMTGYQQVITNQIYHNQIIVFSEPTIG
CCCEEEEECCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCC
AVGIDRASYESVDPTCKGVIVRDVAAVSINRRRRLSLDEYLKRQNIPGISGIDTRALIKK
EEECCCCCCCCCCCCCCCEEEHHHHHHHCCCHHCCCHHHHHHHCCCCCCCCCCHHHHHHH
LRSTGTIKGSIIDPVKDLEHAFDQLRAIVLTNQQVNQVSTPKAFPNPGNGKNVVVVDLGL
HHHCCCCCCCCCCHHHHHHHHHHHHHEEEECCCHHCCCCCCCCCCCCCCCCEEEEEECCC
KYGILRQLSQRDCNVTVLGYSATADSIFSLDPDGVIISTGPGNPRDLPPEVIEMIQIVER
HHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHC
NIPLFAIGLGHEAFAIANGCEIMELSSEHHGSNHPILSVITDEIMYASHGQGFTVDPNSV
CCCEEEEECCCCEEEEECCCEEEEECCCCCCCCCCEEEEEHHHHHEECCCCCEEECCCCC
DRNDLIVTHYDLIDHSVQGLRHRDFPAFSVQFFPDSSPGPTEATELFDEFMEMMTRGGR
CCCCEEEEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12397186 [H]