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The map label for this gene is carB [H]

Identifier: 116492708

GI number: 116492708

Start: 944559

End: 947015

Strand: Direct

Name: carB [H]

Synonym: PEPE_0944

Alternate gene names: 116492708

Gene position: 944559-947015 (Clockwise)

Preceding gene: 116492707

Following gene: 116492710

Centisome position: 51.55

GC content: 38.14

Gene sequence:

>2457_bases
TTGGCAAGATCGCGTGCTAAAAATATTTTGATTATTGGAGCAGGGCCAACACAAATCGGACGTGATACCGAAAGTGAAGC
GGCCGCCGTTCAAATAATCAAAAATTTTGCTGAAACTGATCGTAATTTATTTTATGTCACTAGTGATGCAAATTCAGTGA
TTCCTCGTTTAGATGTTGATGTTAAATTTATTGAAGCCGAATTAACGGTTAACAGCCTGATCCAAATTTTACGAGAAAAT
GAAATAGATGTTATTTTGCCAGTGGCTGGAAATACAGTTGCTTTGCAGCTTTCTGAACAACTAAATGAATCAGGTATTTT
AAAAGAACTCAACGTCCGTACCATGGGGGTAACAACCACTACTATTCATAATATTAACAACCCTGAGTTGATGAACCGCG
TTCTTAAACATATTAAAGAACCAACTATTCCTACTACGGTTGTTGAAAATGTGACAGATGCGTTAAGATTTGCGAAAAGG
GTTGGATTCCCGGTTTTGATTAAGCCCATTGCATCTTTAACTAAAACTAACCGGATTAAGTGTGATAATGAACATCAACT
AATGGAGATGCTTGAATCGGCATTCAAGGTTTCTATTACTAATCAAGTTGCTGTTGAAAAAAGTGTAGTTGGCTATAAAG
AAATTGAAATGATTGTCATTCGTGACGCTGAAGGATCAAAGCTTTTAATTAACGGGGGCGAAAACTTTAATCCAATTGGC
GTTCACTCTGGAGATTCCATTATTTTTAGTCCCACCCAAACACTAACTGACGTCGAATTTCAATCGCTGCGGACGGCTGC
TCTAAAGATAGCTGAAATCTTGAAAATAAAGGGAGTTTGCCACGTTCAATTTGCCTTGGATCCAACGGATGGCAAGCATT
TTATCACCCGGATCAATCCCTTTTTCGATCGATCATCGGCATTTGCTGCTAGGGCAACTGGTTATCCCATTGCCAATGTA
GTTGCCAAGATCTTAATGGGACAAAACTTACGTGAAATTAAACTTTCGGGAGTTCAACGCCATGATTCATTAGCATTGAT
TGAACCCGTGATGGATCACATTGCAGTCCGCTTTCCAACTTGGTCTTTTGCCAGCTTTAAAAATGCTAACCAACAACTTA
ATACCCGAATGAAATCGACGGGTTCGGTAATGGCCTTTGGGCGTAGTGCAGAAGAAGCTTTACAAAAGGCGATTCGTTCA
GTTGATTCAACTACGGCAGCCGAACAAGCAGCCTTAGATGAATCCCAGCTAAGTGAAGCGCAATTAATTAACGTTTTGAT
ACATCCTCGTGCTGGAGAAGTTTATTACTTGTTAGAAGCAATCCGTCGGGGATACACCGTTGATGAGCTGGCTAAGATGA
CGCATATTGACGCTTTCTATTTCTATAAGTTACGTCATTTAGTTAAACTAGAGCGACGACTTAGAGATAATCGTTTTGAC
ATGGCAGCCTTACAAGAAGCCAAGTATTTCGGCTTTGGTGATACGCAAATTGCCCATCTTTGGCAGGTGAGATCTGATGA
AGTCCGCGATTTTCGTATTAAAAAAGCTCATGTATTTGCGACCTATAAGGGTGTGGAACCAACTGCTGGAGAATTTCCTT
ACCTAACTAATACTTACTATTCTACTTTTGAGGATGAGGATGAAGCTGTTCGAACAACTGGTAAAAAGATTCTTGTTCTC
GGAACAGCTAACAGCCAAGTTGGTACTAATAGTGCAGCTGAATATACAACTTTTCAGACAATTCAACGTTGTCAGAAATT
AGGGTACAAGGTTATTTTGATTAACAATAATCCGAATGCGTTAACTTTAATTCCTGATTTAGCGGATAAAATCTATTTAG
AACCACTAACCGTTGAGAATTGCCTGAATATTATTCATCAAGAAAAACCTGATAAAATCATCGCCCAAGGAAAATGGGAA
ATTGTTACCCAATTAGTCTTGCGGAATGTTCAGGTGGAACAGCTTCCTGTTAGTCGAACTACTCGATCAGAAAATATCGA
GGCCGATTACATGGGTGTTATCAGTTCCATCAATGGTTCTATCAACTTATTTGGCGTTGTTGGTATTGATGAAGATAATA
TGTTTATTCCAATTGAAGTTTCGGAAGCATTAAAAGTTAAAGTAGATCCGTTAATTAAAAATGCAAACCAGCATATTACT
AATAATGGTATTTATTCAGTTATTATTAAGGAAAATATGCATCAGCTTCAAATTGTAAGTGTTGTGCCTTTAGCGGTGCA
AGATTTACCATTCTTATCATTTGCTTTTGATCAAGATTTTGCTGGTATAATCACGGAGATTACCTTGAATGGATCAAGTA
AAATCGAAAATCATCCGATAATCCAAGGTGATTATCGCTATTCACATGTTTATCCTTACACTCAATTTGGTCTTAAGAAA
CCGCAAAATCCACCATTCTCCTTAGCAATTGGTGCTGAATTTCAGTCTATAAAATAA

Upstream 100 bases:

>100_bases
TTCAGTGTTCAATTCTTTCCAGATAGTTCTCCTGGTCCAACGGAAGCTACCGAATTGTTTGATGAATTTATGGAAATGAT
GACAAGAGGGGGTCGTTGAT

Downstream 100 bases:

>100_bases
AAAAACCACTTTATGAAAATTTTATTTCATAAAGTGGTTTTCGCGTTTTAAAAGTACCTAAATCATAAATGGACGAATCG
CCTCTAAACTAGGTGTAACT

Product: carbamoyl-phosphate synthase large subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase ammonia chain [H]

Number of amino acids: Translated: 818; Mature: 817

Protein sequence:

>818_residues
MARSRAKNILIIGAGPTQIGRDTESEAAAVQIIKNFAETDRNLFYVTSDANSVIPRLDVDVKFIEAELTVNSLIQILREN
EIDVILPVAGNTVALQLSEQLNESGILKELNVRTMGVTTTTIHNINNPELMNRVLKHIKEPTIPTTVVENVTDALRFAKR
VGFPVLIKPIASLTKTNRIKCDNEHQLMEMLESAFKVSITNQVAVEKSVVGYKEIEMIVIRDAEGSKLLINGGENFNPIG
VHSGDSIIFSPTQTLTDVEFQSLRTAALKIAEILKIKGVCHVQFALDPTDGKHFITRINPFFDRSSAFAARATGYPIANV
VAKILMGQNLREIKLSGVQRHDSLALIEPVMDHIAVRFPTWSFASFKNANQQLNTRMKSTGSVMAFGRSAEEALQKAIRS
VDSTTAAEQAALDESQLSEAQLINVLIHPRAGEVYYLLEAIRRGYTVDELAKMTHIDAFYFYKLRHLVKLERRLRDNRFD
MAALQEAKYFGFGDTQIAHLWQVRSDEVRDFRIKKAHVFATYKGVEPTAGEFPYLTNTYYSTFEDEDEAVRTTGKKILVL
GTANSQVGTNSAAEYTTFQTIQRCQKLGYKVILINNNPNALTLIPDLADKIYLEPLTVENCLNIIHQEKPDKIIAQGKWE
IVTQLVLRNVQVEQLPVSRTTRSENIEADYMGVISSINGSINLFGVVGIDEDNMFIPIEVSEALKVKVDPLIKNANQHIT
NNGIYSVIIKENMHQLQIVSVVPLAVQDLPFLSFAFDQDFAGIITEITLNGSSKIENHPIIQGDYRYSHVYPYTQFGLKK
PQNPPFSLAIGAEFQSIK

Sequences:

>Translated_818_residues
MARSRAKNILIIGAGPTQIGRDTESEAAAVQIIKNFAETDRNLFYVTSDANSVIPRLDVDVKFIEAELTVNSLIQILREN
EIDVILPVAGNTVALQLSEQLNESGILKELNVRTMGVTTTTIHNINNPELMNRVLKHIKEPTIPTTVVENVTDALRFAKR
VGFPVLIKPIASLTKTNRIKCDNEHQLMEMLESAFKVSITNQVAVEKSVVGYKEIEMIVIRDAEGSKLLINGGENFNPIG
VHSGDSIIFSPTQTLTDVEFQSLRTAALKIAEILKIKGVCHVQFALDPTDGKHFITRINPFFDRSSAFAARATGYPIANV
VAKILMGQNLREIKLSGVQRHDSLALIEPVMDHIAVRFPTWSFASFKNANQQLNTRMKSTGSVMAFGRSAEEALQKAIRS
VDSTTAAEQAALDESQLSEAQLINVLIHPRAGEVYYLLEAIRRGYTVDELAKMTHIDAFYFYKLRHLVKLERRLRDNRFD
MAALQEAKYFGFGDTQIAHLWQVRSDEVRDFRIKKAHVFATYKGVEPTAGEFPYLTNTYYSTFEDEDEAVRTTGKKILVL
GTANSQVGTNSAAEYTTFQTIQRCQKLGYKVILINNNPNALTLIPDLADKIYLEPLTVENCLNIIHQEKPDKIIAQGKWE
IVTQLVLRNVQVEQLPVSRTTRSENIEADYMGVISSINGSINLFGVVGIDEDNMFIPIEVSEALKVKVDPLIKNANQHIT
NNGIYSVIIKENMHQLQIVSVVPLAVQDLPFLSFAFDQDFAGIITEITLNGSSKIENHPIIQGDYRYSHVYPYTQFGLKK
PQNPPFSLAIGAEFQSIK
>Mature_817_residues
ARSRAKNILIIGAGPTQIGRDTESEAAAVQIIKNFAETDRNLFYVTSDANSVIPRLDVDVKFIEAELTVNSLIQILRENE
IDVILPVAGNTVALQLSEQLNESGILKELNVRTMGVTTTTIHNINNPELMNRVLKHIKEPTIPTTVVENVTDALRFAKRV
GFPVLIKPIASLTKTNRIKCDNEHQLMEMLESAFKVSITNQVAVEKSVVGYKEIEMIVIRDAEGSKLLINGGENFNPIGV
HSGDSIIFSPTQTLTDVEFQSLRTAALKIAEILKIKGVCHVQFALDPTDGKHFITRINPFFDRSSAFAARATGYPIANVV
AKILMGQNLREIKLSGVQRHDSLALIEPVMDHIAVRFPTWSFASFKNANQQLNTRMKSTGSVMAFGRSAEEALQKAIRSV
DSTTAAEQAALDESQLSEAQLINVLIHPRAGEVYYLLEAIRRGYTVDELAKMTHIDAFYFYKLRHLVKLERRLRDNRFDM
AALQEAKYFGFGDTQIAHLWQVRSDEVRDFRIKKAHVFATYKGVEPTAGEFPYLTNTYYSTFEDEDEAVRTTGKKILVLG
TANSQVGTNSAAEYTTFQTIQRCQKLGYKVILINNNPNALTLIPDLADKIYLEPLTVENCLNIIHQEKPDKIIAQGKWEI
VTQLVLRNVQVEQLPVSRTTRSENIEADYMGVISSINGSINLFGVVGIDEDNMFIPIEVSEALKVKVDPLIKNANQHITN
NGIYSVIIKENMHQLQIVSVVPLAVQDLPFLSFAFDQDFAGIITEITLNGSSKIENHPIIQGDYRYSHVYPYTQFGLKKP
QNPPFSLAIGAEFQSIK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0458

COG function: function code EF; Carbamoylphosphate synthase large subunit (split gene in MJ)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ATP-grasp domains [H]

Homologues:

Organism=Homo sapiens, GI21361331, Length=638, Percent_Identity=31.8181818181818, Blast_Score=353, Evalue=5e-97,
Organism=Homo sapiens, GI169790915, Length=638, Percent_Identity=31.8181818181818, Blast_Score=352, Evalue=6e-97,
Organism=Homo sapiens, GI170295797, Length=566, Percent_Identity=33.0388692579505, Blast_Score=343, Evalue=3e-94,
Organism=Homo sapiens, GI18105007, Length=631, Percent_Identity=30.4278922345483, Blast_Score=322, Evalue=8e-88,
Organism=Escherichia coli, GI1786216, Length=668, Percent_Identity=31.437125748503, Blast_Score=352, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI193204318, Length=636, Percent_Identity=30.3459119496855, Blast_Score=315, Evalue=9e-86,
Organism=Saccharomyces cerevisiae, GI6322569, Length=639, Percent_Identity=32.0813771517997, Blast_Score=315, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6322331, Length=638, Percent_Identity=29.6238244514107, Blast_Score=301, Evalue=2e-82,
Organism=Drosophila melanogaster, GI45555749, Length=633, Percent_Identity=31.911532385466, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24642586, Length=633, Percent_Identity=31.911532385466, Blast_Score=329, Evalue=4e-90,

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006275
- InterPro:   IPR005479
- InterPro:   IPR005483
- InterPro:   IPR005481
- InterPro:   IPR005480
- InterPro:   IPR011607
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF00289 CPSase_L_chain; PF02786 CPSase_L_D2; PF02787 CPSase_L_D3; PF02142 MGS [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 91425; Mature: 91294

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSRAKNILIIGAGPTQIGRDTESEAAAVQIIKNFAETDRNLFYVTSDANSVIPRLDVD
CCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCC
VKFIEAELTVNSLIQILRENEIDVILPVAGNTVALQLSEQLNESGILKELNVRTMGVTTT
EEEEEEHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEHHHCCCCCCEEECCEEEEEEEEE
TIHNINNPELMNRVLKHIKEPTIPTTVVENVTDALRFAKRVGFPVLIKPIASLTKTNRIK
EEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
CDNEHQLMEMLESAFKVSITNQVAVEKSVVGYKEIEMIVIRDAEGSKLLINGGENFNPIG
CCCHHHHHHHHHHHHEEEECCHHHHHHHHCCCEEEEEEEEECCCCCEEEEECCCCCCCCE
VHSGDSIIFSPTQTLTDVEFQSLRTAALKIAEILKIKGVCHVQFALDPTDGKHFITRINP
ECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCEEEEECCH
FFDRSSAFAARATGYPIANVVAKILMGQNLREIKLSGVQRHDSLALIEPVMDHIAVRFPT
HHCCCCCCEECCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHEEECCC
WSFASFKNANQQLNTRMKSTGSVMAFGRSAEEALQKAIRSVDSTTAAEQAALDESQLSEA
CCCHHHCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHH
QLINVLIHPRAGEVYYLLEAIRRGYTVDELAKMTHIDAFYFYKLRHLVKLERRLRDNRFD
EEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
MAALQEAKYFGFGDTQIAHLWQVRSDEVRDFRIKKAHVFATYKGVEPTAGEFPYLTNTYY
HHHHHHHHCCCCCCCHHHHHHHCCCCCCHHEEEEEEEEEEEECCCCCCCCCCCCEECCHH
STFEDEDEAVRTTGKKILVLGTANSQVGTNSAAEYTTFQTIQRCQKLGYKVILINNNPNA
CCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCE
LTLIPDLADKIYLEPLTVENCLNIIHQEKPDKIIAQGKWEIVTQLVLRNVQVEQLPVSRT
EEECCCCCCHHEECCCCHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHCCCCEECCCCCC
TRSENIEADYMGVISSINGSINLFGVVGIDEDNMFIPIEVSEALKVKVDPLIKNANQHIT
CCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCEEEEEEECCCEEEEECHHHHCCCCCCC
NNGIYSVIIKENMHQLQIVSVVPLAVQDLPFLSFAFDQDFAGIITEITLNGSSKIENHPI
CCCEEEEEEECCCCEEEEEEECCHHHHCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCE
IQGDYRYSHVYPYTQFGLKKPQNPPFSLAIGAEFQSIK
EECCCCEEEECCHHHHCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
ARSRAKNILIIGAGPTQIGRDTESEAAAVQIIKNFAETDRNLFYVTSDANSVIPRLDVD
CCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCC
VKFIEAELTVNSLIQILRENEIDVILPVAGNTVALQLSEQLNESGILKELNVRTMGVTTT
EEEEEEHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEHHHCCCCCCEEECCEEEEEEEEE
TIHNINNPELMNRVLKHIKEPTIPTTVVENVTDALRFAKRVGFPVLIKPIASLTKTNRIK
EEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
CDNEHQLMEMLESAFKVSITNQVAVEKSVVGYKEIEMIVIRDAEGSKLLINGGENFNPIG
CCCHHHHHHHHHHHHEEEECCHHHHHHHHCCCEEEEEEEEECCCCCEEEEECCCCCCCCE
VHSGDSIIFSPTQTLTDVEFQSLRTAALKIAEILKIKGVCHVQFALDPTDGKHFITRINP
ECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCEEEEECCH
FFDRSSAFAARATGYPIANVVAKILMGQNLREIKLSGVQRHDSLALIEPVMDHIAVRFPT
HHCCCCCCEECCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHEEECCC
WSFASFKNANQQLNTRMKSTGSVMAFGRSAEEALQKAIRSVDSTTAAEQAALDESQLSEA
CCCHHHCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHH
QLINVLIHPRAGEVYYLLEAIRRGYTVDELAKMTHIDAFYFYKLRHLVKLERRLRDNRFD
EEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
MAALQEAKYFGFGDTQIAHLWQVRSDEVRDFRIKKAHVFATYKGVEPTAGEFPYLTNTYY
HHHHHHHHCCCCCCCHHHHHHHCCCCCCHHEEEEEEEEEEEECCCCCCCCCCCCEECCHH
STFEDEDEAVRTTGKKILVLGTANSQVGTNSAAEYTTFQTIQRCQKLGYKVILINNNPNA
CCCCCCHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCE
LTLIPDLADKIYLEPLTVENCLNIIHQEKPDKIIAQGKWEIVTQLVLRNVQVEQLPVSRT
EEECCCCCCHHEECCCCHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHCCCCEECCCCCC
TRSENIEADYMGVISSINGSINLFGVVGIDEDNMFIPIEVSEALKVKVDPLIKNANQHIT
CCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCEEEEEEECCCEEEEECHHHHCCCCCCC
NNGIYSVIIKENMHQLQIVSVVPLAVQDLPFLSFAFDQDFAGIITEITLNGSSKIENHPI
CCCEEEEEEECCCCEEEEEEECCHHHHCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCE
IQGDYRYSHVYPYTQFGLKKPQNPPFSLAIGAEFQSIK
EECCCCEEEECCHHHHCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA