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The map label for this gene is rsmF [H]

Identifier: 116492681

GI number: 116492681

Start: 914798

End: 916174

Strand: Direct

Name: rsmF [H]

Synonym: PEPE_0916

Alternate gene names: 116492681

Gene position: 914798-916174 (Clockwise)

Preceding gene: 116492679

Following gene: 116492682

Centisome position: 49.92

GC content: 41.68

Gene sequence:

>1377_bases
ATGCAACTACCAGTTGATTTTAAGGAAAAATATGAAAAATTACTTGGGGATCAAGCCGAAGCTTTTTTAGCGAGCTTCGA
TAATCCTGTTCAAAAGGGATTTCGGATTAATCCGCTTAAATATCACGGACATCTTCATGAACATGCAATGCAACAATCTG
TGGGTTATTCAGCTTGGGGCTATTTTGGTAAAGTACGTGGGAAAACCATCGATCATCAAAGTGGACTGGTTTACAGCCAG
GAACCAAGTGCCATGCTGGTCGGTGAATTTGCCCATCCTAAACCAGGTCAAAAAGTGTTAGATTTGTGCGCCGCACCAGG
TGGAAAGACTACTTATTTAGCTAGTTTCATGGATCAACAAGGGTTATTAGTTTCTAATGAAATCAATCGAGGCCGAGCTA
AAATCTTGGCTGAGAACGTTGAACGCTTTGGTATTCAGAATGCGGTGATTTTAAATGAGTCACCTGATCGAATGGAAGAT
CGTTTTGCCAATTATTTCGATGTGATTGTGGTGGATGCGCCTTGTTCTGGGGAAGGAATGTTTAGAAAAGATCCTGCAGC
CATGGAATATTGGAATAAAGATTACCCATCTGAGTGCGCTAATCGCCAAAGAGAAATTTTAAAGAGCGCGATGAAGATGT
TGGCCCCTAAGGGAACTTTAGTTTATTCGACGTGTACCTTTGCTCCTGAAGAAGATGAACAAATTATCGCTTGGTTATTA
GATAACTACGAAAATCTAGCATTGGTGGATGTCCCTAAACAAGATGACATGGATGCAGGGCGTCCGGAGTGGGCCGACGG
CAATCCAGAACTAACCAAGGCGGTACGTTTATTCCCTCATCATTTTCAGGGTGAAGGACATTTTATGGCAAAACTGGTCA
ATCACGGGATGGAGACGCCCACTGAAAAGCCCCGAGCTAAGAAGAAAAAGAAACAATCAAGTAATTCAAAGTCCCTAAAC
AAGACCCAGGTGAAATTGTGGGAGACTTTTGCTAAAGCGTTTTTGAAAGATTCAAATTATTTCGATTTAGCTCATTTAAT
GGTTCAAAAAGATCGCTTATATTATCAAAGTGAGCGTTTAGATTTAGAAGGATTAAGGTATATCAAGCCTGGATTAGAGC
TGGGAGAATTTAAGAAAAATCGTTTTGAACCCAGCCAGAGCCTTTTAATGGCCCTCCCCGTTGATGAATTCCAACAAGTT
GTTAAAATTACAGCTGAGGAGTGGCGTCAATATGTACATGGCGACGTTCTGTCGACAACTACTGCTGGTAAAGGTTGGGT
AGCCGTAGCTGTAGATGACACAGTGGTTGGACCTGGTAAGTTAGTACAAGGTACGGTTAAAAACTTCTATCCCAAAGGGT
TACGGATGAACTTTTGA

Upstream 100 bases:

>100_bases
AAGTTAAATGAATTGTTAAGGTGGCTCAACCCGTTTTTTTTCTGTATAATTAAGTCCAGTTTTATGGCGAATGAATAAAG
AATAGAAGGAATATTTAAAA

Downstream 100 bases:

>100_bases
TTTACTAAAGTGAATAAAAATTAATTTAAAAAATGATCTCTAGAATTAGGACTGGTTAAGCTAATTTTAGGGCTCTTTTT
TATTTTTAGCTTTTTAAGAT

Product: tRNA and rRNA cytosine-C5-methylase

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MQLPVDFKEKYEKLLGDQAEAFLASFDNPVQKGFRINPLKYHGHLHEHAMQQSVGYSAWGYFGKVRGKTIDHQSGLVYSQ
EPSAMLVGEFAHPKPGQKVLDLCAAPGGKTTYLASFMDQQGLLVSNEINRGRAKILAENVERFGIQNAVILNESPDRMED
RFANYFDVIVVDAPCSGEGMFRKDPAAMEYWNKDYPSECANRQREILKSAMKMLAPKGTLVYSTCTFAPEEDEQIIAWLL
DNYENLALVDVPKQDDMDAGRPEWADGNPELTKAVRLFPHHFQGEGHFMAKLVNHGMETPTEKPRAKKKKKQSSNSKSLN
KTQVKLWETFAKAFLKDSNYFDLAHLMVQKDRLYYQSERLDLEGLRYIKPGLELGEFKKNRFEPSQSLLMALPVDEFQQV
VKITAEEWRQYVHGDVLSTTTAGKGWVAVAVDDTVVGPGKLVQGTVKNFYPKGLRMNF

Sequences:

>Translated_458_residues
MQLPVDFKEKYEKLLGDQAEAFLASFDNPVQKGFRINPLKYHGHLHEHAMQQSVGYSAWGYFGKVRGKTIDHQSGLVYSQ
EPSAMLVGEFAHPKPGQKVLDLCAAPGGKTTYLASFMDQQGLLVSNEINRGRAKILAENVERFGIQNAVILNESPDRMED
RFANYFDVIVVDAPCSGEGMFRKDPAAMEYWNKDYPSECANRQREILKSAMKMLAPKGTLVYSTCTFAPEEDEQIIAWLL
DNYENLALVDVPKQDDMDAGRPEWADGNPELTKAVRLFPHHFQGEGHFMAKLVNHGMETPTEKPRAKKKKKQSSNSKSLN
KTQVKLWETFAKAFLKDSNYFDLAHLMVQKDRLYYQSERLDLEGLRYIKPGLELGEFKKNRFEPSQSLLMALPVDEFQQV
VKITAEEWRQYVHGDVLSTTTAGKGWVAVAVDDTVVGPGKLVQGTVKNFYPKGLRMNF
>Mature_458_residues
MQLPVDFKEKYEKLLGDQAEAFLASFDNPVQKGFRINPLKYHGHLHEHAMQQSVGYSAWGYFGKVRGKTIDHQSGLVYSQ
EPSAMLVGEFAHPKPGQKVLDLCAAPGGKTTYLASFMDQQGLLVSNEINRGRAKILAENVERFGIQNAVILNESPDRMED
RFANYFDVIVVDAPCSGEGMFRKDPAAMEYWNKDYPSECANRQREILKSAMKMLAPKGTLVYSTCTFAPEEDEQIIAWLL
DNYENLALVDVPKQDDMDAGRPEWADGNPELTKAVRLFPHHFQGEGHFMAKLVNHGMETPTEKPRAKKKKKQSSNSKSLN
KTQVKLWETFAKAFLKDSNYFDLAHLMVQKDRLYYQSERLDLEGLRYIKPGLELGEFKKNRFEPSQSLLMALPVDEFQQV
VKITAEEWRQYVHGDVLSTTTAGKGWVAVAVDDTVVGPGKLVQGTVKNFYPKGLRMNF

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=332, Percent_Identity=28.3132530120482, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI76150623, Length=332, Percent_Identity=28.3132530120482, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI32698918, Length=179, Percent_Identity=35.195530726257, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI301336155, Length=361, Percent_Identity=25.207756232687, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI39995082, Length=337, Percent_Identity=25.8160237388724, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI11545785, Length=277, Percent_Identity=29.9638989169675, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI270288816, Length=266, Percent_Identity=25.187969924812, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI23199998, Length=265, Percent_Identity=26.0377358490566, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI8922322, Length=172, Percent_Identity=28.4883720930233, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI270288818, Length=172, Percent_Identity=28.4883720930233, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI87081985, Length=457, Percent_Identity=28.8840262582057, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI2367212, Length=226, Percent_Identity=31.858407079646, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17536757, Length=328, Percent_Identity=30.1829268292683, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI25143471, Length=192, Percent_Identity=32.2916666666667, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI115534021, Length=168, Percent_Identity=30.952380952381, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71998419, Length=168, Percent_Identity=30.952380952381, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324268, Length=263, Percent_Identity=30.4182509505703, Blast_Score=123, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6319447, Length=187, Percent_Identity=29.9465240641711, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI22024126, Length=255, Percent_Identity=31.7647058823529, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI21356513, Length=184, Percent_Identity=30.4347826086957, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24668781, Length=184, Percent_Identity=30.4347826086957, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21355201, Length=232, Percent_Identity=26.7241379310345, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356579, Length=190, Percent_Identity=29.4736842105263, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920494, Length=164, Percent_Identity=29.2682926829268, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 51889; Mature: 51889

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLPVDFKEKYEKLLGDQAEAFLASFDNPVQKGFRINPLKYHGHLHEHAMQQSVGYSAWG
CCCCCCHHHHHHHHHCCHHHHHHHHCCCHHHCCCEECCHHHCCHHHHHHHHHHCCCHHHH
YFGKVRGKTIDHQSGLVYSQEPSAMLVGEFAHPKPGQKVLDLCAAPGGKTTYLASFMDQQ
HHHHHCCCEECCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCC
GLLVSNEINRGRAKILAENVERFGIQNAVILNESPDRMEDRFANYFDVIVVDAPCSGEGM
CCEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCEEEEEEEECCCCCCCC
FRKDPAAMEYWNKDYPSECANRQREILKSAMKMLAPKGTLVYSTCTFAPEEDEQIIAWLL
CCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHH
DNYENLALVDVPKQDDMDAGRPEWADGNPELTKAVRLFPHHFQGEGHFMAKLVNHGMETP
CCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHCCCCCHHHHHHHHCCCCCC
TEKPRAKKKKKQSSNSKSLNKTQVKLWETFAKAFLKDSNYFDLAHLMVQKDRLYYQSERL
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
DLEGLRYIKPGLELGEFKKNRFEPSQSLLMALPVDEFQQVVKITAEEWRQYVHGDVLSTT
CCCCHHHHCCCCCHHHHHHHHCCCCHHHEEECCHHHHHHHHHHHHHHHHHHHCCCHHEEC
TAGKGWVAVAVDDTVVGPGKLVQGTVKNFYPKGLRMNF
CCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCEECC
>Mature Secondary Structure
MQLPVDFKEKYEKLLGDQAEAFLASFDNPVQKGFRINPLKYHGHLHEHAMQQSVGYSAWG
CCCCCCHHHHHHHHHCCHHHHHHHHCCCHHHCCCEECCHHHCCHHHHHHHHHHCCCHHHH
YFGKVRGKTIDHQSGLVYSQEPSAMLVGEFAHPKPGQKVLDLCAAPGGKTTYLASFMDQQ
HHHHHCCCEECCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCC
GLLVSNEINRGRAKILAENVERFGIQNAVILNESPDRMEDRFANYFDVIVVDAPCSGEGM
CCEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCEEEEEEEECCCCCCCC
FRKDPAAMEYWNKDYPSECANRQREILKSAMKMLAPKGTLVYSTCTFAPEEDEQIIAWLL
CCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHH
DNYENLALVDVPKQDDMDAGRPEWADGNPELTKAVRLFPHHFQGEGHFMAKLVNHGMETP
CCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHCCCCCHHHHHHHHCCCCCC
TEKPRAKKKKKQSSNSKSLNKTQVKLWETFAKAFLKDSNYFDLAHLMVQKDRLYYQSERL
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
DLEGLRYIKPGLELGEFKKNRFEPSQSLLMALPVDEFQQVVKITAEEWRQYVHGDVLSTT
CCCCHHHHCCCCCHHHHHHHHCCCCHHHEEECCHHHHHHHHHHHHHHHHHHHCCCHHEEC
TAGKGWVAVAVDDTVVGPGKLVQGTVKNFYPKGLRMNF
CCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA