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The map label for this gene is polA [H]

Identifier: 116492456

GI number: 116492456

Start: 728250

End: 730913

Strand: Direct

Name: polA [H]

Synonym: PEPE_0688

Alternate gene names: 116492456

Gene position: 728250-730913 (Clockwise)

Preceding gene: 116492455

Following gene: 116492457

Centisome position: 39.74

GC content: 35.96

Gene sequence:

>2664_bases
ATGGCAAAAGATAAACTTTTATTGATTGATGGAAATAGTATTGCTTTTCGGTCTTTTTTTGCGTTACATAATTCTTTAAG
TAAATTTGTAAATAAAAATGGATTACATACTAATGCAATCTTTGGTTTCAACAAAATGTTAGATAGTATTTTAGAACAAT
ACCAGCCGACAGCGGCCTTGGTGGCCTTCGATGCTGGTAAAACCACTTTTAGAAATGGAAAATTTGATAATTATAAAGGT
GGTCGGTCTAAAACACCACCTGAACTATCAGAGCAAATGCCTTATTTACGTGAATTACTTACAGGATATGGAATAAAATC
TTACGAGCTTCCTAATTATGAAGCTGATGACATTATTGGAACACTTGCTAAAGAAGCTGAAGCCCAAGATTATGCGGTAG
TTGTGGTTACGGGAGATCGTGACTTAACCCAATTGAGTAGCGAAAAAACAATCGTCGCCGTTACGAAAAAAGGGGTAAGC
GAAGTAGAAGAGTACACACCAGCGTATGTCGAAGAAAAATTAGGCGTAACACCTAAACAAATTATTGACATTAAAGGCTT
AGCTGGAGACACATCGGATAATTATCCTGGAGTAACTAAAGTTGGTGAAAAGACGGCCATTAAGTTGTTAAAACAATTTG
ATTCATTGGAGAATTTGTATGAGCATCTTGATGAATTAAAACCTAGCAAGATGAAGGAGCATTTGATTGAAGATCGAGAC
AATGCTTTCATATCAAAAGATCTAGCAACTATTAGACGGGATGCACCGATAGAAATTAGTCTCGATGATGTGCAGTATGC
CGGTATTAACGAAGAATATTTGAGAGAATTTTACCACCAAATGAACTTTAAGTCCTTTTTAAGTAAATTAGATCAAACGG
CAACTCAAGATGGAGTTGAGGCCGTGAAAAAAGAGGTTGTTTTTAACGTTTTAAATAAGGACAACGTGGAACAACTCCAA
TCATTAAAAGGTGAAATTGATTTTTATTTGGAAATGTTGGACAAAAACTATCATTTGTCTGATTTTACGGGATTTGTGAT
CGGAAATGCCGATAACATTTGGGTTAGTGATGATGTAGAACTGTTGAACAATTCAAAAATCAAAGCTATTTTGGAAGATC
CCAAAATTAAGATAAATGTATTTGATAGTAAGCGGACGTATGTTGGCTTACATCGACTAGGGATTAAGCTAACGGCGGTT
AACTTTGATATATTGCTTCAATCGTATTTGTTAGATACTAATGATAATTCTAATGACCTAGGTCAACTTGCTCATGAGCA
TGATTACGATGCGATTGATACAGACGATGATGTTTATGGCAAAGGCGCTAAGCGTGCGGTTCCAGAGCAAACAGAATTGT
TTAACCACCTTGCACGTAAATTAGCAGCTATTAACTTTTTAGGTAAACAATTGGACCAAGAATTAGTAAAAAATGAACAA
AATAATTTATATTGGGATATTGAATTACCACTCGCAATTGTATTAGCCAAGATGGAAATTAGCGGTATTAAAGTTGACCG
CAACCGCTTAAATCAAATGCAAAGCGAGTTTACTGAACGCTTAAGTGAAATTGAAACCAATATCTACAACGAAGCGGGAG
AGGAATTTAATCTTAATTCGCCCAAGCAACTCGGAGTAGTGCTGTTTGAAAAGTTAAAATTACCAATCATTAAGAAAACT
AAAACAGGGTATTCAACTGCGGTAGACGTCTTAGAGAAATTACAAGGCGAGTCACCAATTATTGATAATATTTTGAACTA
CCGTACGCTTTCAAAAATTAAATCAACTTATGTTGATGGGCTTTTAAAAGTAATTCATACGACTGATGAAAAAGTGCATA
CAACCTATGTACAAACTTTGACCCAAACGGGCCGACTATCTTCCATTGACCCTAATTTACAAAATATTCCGGTGCGCATT
GAAGAGGGTAGGAAGATTCGCCAAGCCTTTGTTCCAGCTCGTGAAGGGTGGCATATTTTATCATCGGATTACTCACAAAT
TGAATTACGCGTATTAGCACATATTACAGGTGATCAGAATTTACAGGAAGCATTTAAAAATGGCGAAGATATTCATGCTA
CAACTGCAGTTAAAATATTTGGATTAAAGGATGCTAGCGAGGTAACTCCTAATATCCGACGTCAAGCTAAAGCAGTTAAT
TTTGGAATTGTGTATGGTATCAGTGACTATGGTTTGTCCCAAAACATTGGAATTTCTAGGAAAGATGCCAAGAAATTTAT
CGAAACATACTTTAAGGAATTTCCTGGTGTAAAAAAATATATTGATGAATCAGTTCAGTTAGCTAAAGAACAAGGCTACG
TAGAAACAATTGCGCACCGTCGACGTTACTTACCAGACATTAATTCGTCTAGCTTTAATTTACGTTCATTTGCGGAAAGA
ACCGCTATGAATACGCCTATTCAAGGTAGTGCTGCTGATATTATTAAGATTGCGATGATCGATATGGAAAATGAATTAGA
AAAGCGTAACTTGAAGAGTAGAATGTTACTCCAAGTGCACGATGAATTGATTTTTGAAGTTCCGGATGAAGAAATGACGT
TAATGAAAGAATTAGTACCGAAGGTTATGGACTCAGCAGTTAAATTGGATGTTCCACTTAAAGTTGGAGCAGCTTGGGGA
AATACGTGGTACGACGCTAAATAA

Upstream 100 bases:

>100_bases
AATAAAAATTTTATAAATACAAATAAACCGAAGGTCAATCTTTTTCACGTTGACCTTCGGTTTTTGGTATCATTAAAGAG
GATAGAAAACGGAGGAAATT

Downstream 100 bases:

>100_bases
CGGGGGAGAAGTATGCCTGAATTACCAGAAGTTGAAACAGTTCGTAGAGGCCTAGCAGCCTTAGTTGAAGGAAAAATAGT
TACTAATGTTGTAGTACGTT

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 887; Mature: 886

Protein sequence:

>887_residues
MAKDKLLLIDGNSIAFRSFFALHNSLSKFVNKNGLHTNAIFGFNKMLDSILEQYQPTAALVAFDAGKTTFRNGKFDNYKG
GRSKTPPELSEQMPYLRELLTGYGIKSYELPNYEADDIIGTLAKEAEAQDYAVVVVTGDRDLTQLSSEKTIVAVTKKGVS
EVEEYTPAYVEEKLGVTPKQIIDIKGLAGDTSDNYPGVTKVGEKTAIKLLKQFDSLENLYEHLDELKPSKMKEHLIEDRD
NAFISKDLATIRRDAPIEISLDDVQYAGINEEYLREFYHQMNFKSFLSKLDQTATQDGVEAVKKEVVFNVLNKDNVEQLQ
SLKGEIDFYLEMLDKNYHLSDFTGFVIGNADNIWVSDDVELLNNSKIKAILEDPKIKINVFDSKRTYVGLHRLGIKLTAV
NFDILLQSYLLDTNDNSNDLGQLAHEHDYDAIDTDDDVYGKGAKRAVPEQTELFNHLARKLAAINFLGKQLDQELVKNEQ
NNLYWDIELPLAIVLAKMEISGIKVDRNRLNQMQSEFTERLSEIETNIYNEAGEEFNLNSPKQLGVVLFEKLKLPIIKKT
KTGYSTAVDVLEKLQGESPIIDNILNYRTLSKIKSTYVDGLLKVIHTTDEKVHTTYVQTLTQTGRLSSIDPNLQNIPVRI
EEGRKIRQAFVPAREGWHILSSDYSQIELRVLAHITGDQNLQEAFKNGEDIHATTAVKIFGLKDASEVTPNIRRQAKAVN
FGIVYGISDYGLSQNIGISRKDAKKFIETYFKEFPGVKKYIDESVQLAKEQGYVETIAHRRRYLPDINSSSFNLRSFAER
TAMNTPIQGSAADIIKIAMIDMENELEKRNLKSRMLLQVHDELIFEVPDEEMTLMKELVPKVMDSAVKLDVPLKVGAAWG
NTWYDAK

Sequences:

>Translated_887_residues
MAKDKLLLIDGNSIAFRSFFALHNSLSKFVNKNGLHTNAIFGFNKMLDSILEQYQPTAALVAFDAGKTTFRNGKFDNYKG
GRSKTPPELSEQMPYLRELLTGYGIKSYELPNYEADDIIGTLAKEAEAQDYAVVVVTGDRDLTQLSSEKTIVAVTKKGVS
EVEEYTPAYVEEKLGVTPKQIIDIKGLAGDTSDNYPGVTKVGEKTAIKLLKQFDSLENLYEHLDELKPSKMKEHLIEDRD
NAFISKDLATIRRDAPIEISLDDVQYAGINEEYLREFYHQMNFKSFLSKLDQTATQDGVEAVKKEVVFNVLNKDNVEQLQ
SLKGEIDFYLEMLDKNYHLSDFTGFVIGNADNIWVSDDVELLNNSKIKAILEDPKIKINVFDSKRTYVGLHRLGIKLTAV
NFDILLQSYLLDTNDNSNDLGQLAHEHDYDAIDTDDDVYGKGAKRAVPEQTELFNHLARKLAAINFLGKQLDQELVKNEQ
NNLYWDIELPLAIVLAKMEISGIKVDRNRLNQMQSEFTERLSEIETNIYNEAGEEFNLNSPKQLGVVLFEKLKLPIIKKT
KTGYSTAVDVLEKLQGESPIIDNILNYRTLSKIKSTYVDGLLKVIHTTDEKVHTTYVQTLTQTGRLSSIDPNLQNIPVRI
EEGRKIRQAFVPAREGWHILSSDYSQIELRVLAHITGDQNLQEAFKNGEDIHATTAVKIFGLKDASEVTPNIRRQAKAVN
FGIVYGISDYGLSQNIGISRKDAKKFIETYFKEFPGVKKYIDESVQLAKEQGYVETIAHRRRYLPDINSSSFNLRSFAER
TAMNTPIQGSAADIIKIAMIDMENELEKRNLKSRMLLQVHDELIFEVPDEEMTLMKELVPKVMDSAVKLDVPLKVGAAWG
NTWYDAK
>Mature_886_residues
AKDKLLLIDGNSIAFRSFFALHNSLSKFVNKNGLHTNAIFGFNKMLDSILEQYQPTAALVAFDAGKTTFRNGKFDNYKGG
RSKTPPELSEQMPYLRELLTGYGIKSYELPNYEADDIIGTLAKEAEAQDYAVVVVTGDRDLTQLSSEKTIVAVTKKGVSE
VEEYTPAYVEEKLGVTPKQIIDIKGLAGDTSDNYPGVTKVGEKTAIKLLKQFDSLENLYEHLDELKPSKMKEHLIEDRDN
AFISKDLATIRRDAPIEISLDDVQYAGINEEYLREFYHQMNFKSFLSKLDQTATQDGVEAVKKEVVFNVLNKDNVEQLQS
LKGEIDFYLEMLDKNYHLSDFTGFVIGNADNIWVSDDVELLNNSKIKAILEDPKIKINVFDSKRTYVGLHRLGIKLTAVN
FDILLQSYLLDTNDNSNDLGQLAHEHDYDAIDTDDDVYGKGAKRAVPEQTELFNHLARKLAAINFLGKQLDQELVKNEQN
NLYWDIELPLAIVLAKMEISGIKVDRNRLNQMQSEFTERLSEIETNIYNEAGEEFNLNSPKQLGVVLFEKLKLPIIKKTK
TGYSTAVDVLEKLQGESPIIDNILNYRTLSKIKSTYVDGLLKVIHTTDEKVHTTYVQTLTQTGRLSSIDPNLQNIPVRIE
EGRKIRQAFVPAREGWHILSSDYSQIELRVLAHITGDQNLQEAFKNGEDIHATTAVKIFGLKDASEVTPNIRRQAKAVNF
GIVYGISDYGLSQNIGISRKDAKKFIETYFKEFPGVKKYIDESVQLAKEQGYVETIAHRRRYLPDINSSSFNLRSFAERT
AMNTPIQGSAADIIKIAMIDMENELEKRNLKSRMLLQVHDELIFEVPDEEMTLMKELVPKVMDSAVKLDVPLKVGAAWGN
TWYDAK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=519, Percent_Identity=31.4065510597302, Blast_Score=232, Evalue=1e-60,
Organism=Homo sapiens, GI139394648, Length=646, Percent_Identity=27.3993808049536, Blast_Score=216, Evalue=7e-56,
Organism=Escherichia coli, GI1790294, Length=940, Percent_Identity=37.5531914893617, Blast_Score=530, Evalue=1e-151,
Organism=Escherichia coli, GI226510970, Length=221, Percent_Identity=30.7692307692308, Blast_Score=117, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI86563272, Length=397, Percent_Identity=29.9748110831234, Blast_Score=179, Evalue=5e-45,
Organism=Drosophila melanogaster, GI17933644, Length=419, Percent_Identity=34.8448687350835, Blast_Score=250, Evalue=4e-66,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 100417; Mature: 100286

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDKLLLIDGNSIAFRSFFALHNSLSKFVNKNGLHTNAIFGFNKMLDSILEQYQPTAAL
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEE
VAFDAGKTTFRNGKFDNYKGGRSKTPPELSEQMPYLRELLTGYGIKSYELPNYEADDIIG
EEEECCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHH
TLAKEAEAQDYAVVVVTGDRDLTQLSSEKTIVAVTKKGVSEVEEYTPAYVEEKLGVTPKQ
HHHHHCCCCCEEEEEEECCCHHHHHCCCCEEEEEEHHHHHHHHHHCHHHHHHHCCCCHHH
IIDIKGLAGDTSDNYPGVTKVGEKTAIKLLKQFDSLENLYEHLDELKPSKMKEHLIEDRD
HEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
NAFISKDLATIRRDAPIEISLDDVQYAGINEEYLREFYHQMNFKSFLSKLDQTATQDGVE
CHHHHHHHHHHHCCCCCEEEECCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AVKKEVVFNVLNKDNVEQLQSLKGEIDFYLEMLDKNYHLSDFTGFVIGNADNIWVSDDVE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEECCCHH
LLNNSKIKAILEDPKIKINVFDSKRTYVGLHRLGIKLTAVNFDILLQSYLLDTNDNSNDL
HHCCCCEEEEECCCCEEEEEECCCCCEEEHHHHCEEEEEEHHHHHHHHHHHCCCCCCHHH
GQLAHEHDYDAIDTDDDVYGKGAKRAVPEQTELFNHLARKLAAINFLGKQLDQELVKNEQ
HHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NNLYWDIELPLAIVLAKMEISGIKVDRNRLNQMQSEFTERLSEIETNIYNEAGEEFNLNS
CCEEEEEECCHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PKQLGVVLFEKLKLPIIKKTKTGYSTAVDVLEKLQGESPIIDNILNYRTLSKIKSTYVDG
CHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LLKVIHTTDEKVHTTYVQTLTQTGRLSSIDPNLQNIPVRIEEGRKIRQAFVPAREGWHIL
HHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCEEE
SSDYSQIELRVLAHITGDQNLQEAFKNGEDIHATTAVKIFGLKDASEVTPNIRRQAKAVN
CCCCHHEEEEEEEEECCCHHHHHHHCCCCCEEEEEEEEEEECCCHHHCCHHHHHHHHHCC
FGIVYGISDYGLSQNIGISRKDAKKFIETYFKEFPGVKKYIDESVQLAKEQGYVETIAHR
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHH
RRYLPDINSSSFNLRSFAERTAMNTPIQGSAADIIKIAMIDMENELEKRNLKSRMLLQVH
HHHCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHEEEEEEECCHHHHHHHHHHHHHHHHHH
DELIFEVPDEEMTLMKELVPKVMDSAVKLDVPLKVGAAWGNTWYDAK
HHHHHCCCCHHHHHHHHHHHHHHHHHEEECCCEEECCCCCCCCCCCC
>Mature Secondary Structure 
AKDKLLLIDGNSIAFRSFFALHNSLSKFVNKNGLHTNAIFGFNKMLDSILEQYQPTAAL
CCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEE
VAFDAGKTTFRNGKFDNYKGGRSKTPPELSEQMPYLRELLTGYGIKSYELPNYEADDIIG
EEEECCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHH
TLAKEAEAQDYAVVVVTGDRDLTQLSSEKTIVAVTKKGVSEVEEYTPAYVEEKLGVTPKQ
HHHHHCCCCCEEEEEEECCCHHHHHCCCCEEEEEEHHHHHHHHHHCHHHHHHHCCCCHHH
IIDIKGLAGDTSDNYPGVTKVGEKTAIKLLKQFDSLENLYEHLDELKPSKMKEHLIEDRD
HEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
NAFISKDLATIRRDAPIEISLDDVQYAGINEEYLREFYHQMNFKSFLSKLDQTATQDGVE
CHHHHHHHHHHHCCCCCEEEECCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AVKKEVVFNVLNKDNVEQLQSLKGEIDFYLEMLDKNYHLSDFTGFVIGNADNIWVSDDVE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEECCCHH
LLNNSKIKAILEDPKIKINVFDSKRTYVGLHRLGIKLTAVNFDILLQSYLLDTNDNSNDL
HHCCCCEEEEECCCCEEEEEECCCCCEEEHHHHCEEEEEEHHHHHHHHHHHCCCCCCHHH
GQLAHEHDYDAIDTDDDVYGKGAKRAVPEQTELFNHLARKLAAINFLGKQLDQELVKNEQ
HHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NNLYWDIELPLAIVLAKMEISGIKVDRNRLNQMQSEFTERLSEIETNIYNEAGEEFNLNS
CCEEEEEECCHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PKQLGVVLFEKLKLPIIKKTKTGYSTAVDVLEKLQGESPIIDNILNYRTLSKIKSTYVDG
CHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LLKVIHTTDEKVHTTYVQTLTQTGRLSSIDPNLQNIPVRIEEGRKIRQAFVPAREGWHIL
HHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCEEE
SSDYSQIELRVLAHITGDQNLQEAFKNGEDIHATTAVKIFGLKDASEVTPNIRRQAKAVN
CCCCHHEEEEEEEEECCCHHHHHHHCCCCCEEEEEEEEEEECCCHHHCCHHHHHHHHHCC
FGIVYGISDYGLSQNIGISRKDAKKFIETYFKEFPGVKKYIDESVQLAKEQGYVETIAHR
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHH
RRYLPDINSSSFNLRSFAERTAMNTPIQGSAADIIKIAMIDMENELEKRNLKSRMLLQVH
HHHCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHEEEEEEECCHHHHHHHHHHHHHHHHHH
DELIFEVPDEEMTLMKELVPKVMDSAVKLDVPLKVGAAWGNTWYDAK
HHHHHCCCCHHHHHHHHHHHHHHHHHEEECCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]