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The map label for this gene is mutM

Identifier: 116492457

GI number: 116492457

Start: 730926

End: 731753

Strand: Direct

Name: mutM

Synonym: PEPE_0689

Alternate gene names: 116492457

Gene position: 730926-731753 (Clockwise)

Preceding gene: 116492456

Following gene: 116492458

Centisome position: 39.89

GC content: 40.7

Gene sequence:

>828_bases
ATGCCTGAATTACCAGAAGTTGAAACAGTTCGTAGAGGCCTAGCAGCCTTAGTTGAAGGAAAAATAGTTACTAATGTTGT
AGTACGTTATTCAAAAATGGTCTCACCAAAAGCGGAAATTTTTGCAGAAGAACTAGAGGGTAAAAAAATTCTTAATGTTC
GACGACGCGGTAAATATCTATTAATTGATTTTAGTGGAGATTACACGATGGTTTCTCATCTTCGCATGGAGGGTAAATAC
TCTGTAGTTGATCGTCGTGAAGAGTATGGCAAGCATGATCACGTTATTTTCGAGCTTGATGATGGAAAAGATCTTCGGTA
CAACGACACTAGAAAATTCGGTAGGATGAATTTGGTACCCACGGGTGAGGAATTACAAGTGGGCGGTTTAAAAACGATTG
GACCAGAACCAACGCCTGAGACCCTTACCTTAGAATATTTAACCCATCAGCTACGGAATCGAAAACGTGGAATGAAAAGT
TTTCTATTGGATCAGTCCATGATTGCTGGTTTAGGCAATATCTACGCTGATGAAGTATTGTGGCTAAGCAAAATTCATCC
TCAGCAGATTTCTAATACTTTAACTGATGAAGAAATTGCAATTTTGCGTGAATCAATTTTTGAAGAGTTGCAATTGGCGA
TTGAAGCTAAAGGAACGACGGTTTTTTCTTATTTGAATGCGGATGGTCATGCGGGAAGTTTTCAAAACCAGTTGCATGTA
TACCATCGCCAAGGTTTGCCGTGTCAGCGATGCGGGACACCCATTGAACGGATTAAAGTGGCACAACGTGGGACCCATTT
TTGCCCCCATTGCCAAGTTTTGAGGTAG

Upstream 100 bases:

>100_bases
ACCGAAGGTTATGGACTCAGCAGTTAAATTGGATGTTCCACTTAAAGTTGGAGCAGCTTGGGGAAATACGTGGTACGACG
CTAAATAACGGGGGAGAAGT

Downstream 100 bases:

>100_bases
TGATATGACAGTGATAGTTGGTCTAACTGGCGGGATTGCAATGGGTAAAACGACGATTAGCCAATTTTTGAAATCTAAAG
CAATTCCGGTAGTTGATGCT

Product: formamidopyrimidine-DNA glycosylase / DNA-(apurinic or apyrimidinic site) lyase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MPELPEVETVRRGLAALVEGKIVTNVVVRYSKMVSPKAEIFAEELEGKKILNVRRRGKYLLIDFSGDYTMVSHLRMEGKY
SVVDRREEYGKHDHVIFELDDGKDLRYNDTRKFGRMNLVPTGEELQVGGLKTIGPEPTPETLTLEYLTHQLRNRKRGMKS
FLLDQSMIAGLGNIYADEVLWLSKIHPQQISNTLTDEEIAILRESIFEELQLAIEAKGTTVFSYLNADGHAGSFQNQLHV
YHRQGLPCQRCGTPIERIKVAQRGTHFCPHCQVLR

Sequences:

>Translated_275_residues
MPELPEVETVRRGLAALVEGKIVTNVVVRYSKMVSPKAEIFAEELEGKKILNVRRRGKYLLIDFSGDYTMVSHLRMEGKY
SVVDRREEYGKHDHVIFELDDGKDLRYNDTRKFGRMNLVPTGEELQVGGLKTIGPEPTPETLTLEYLTHQLRNRKRGMKS
FLLDQSMIAGLGNIYADEVLWLSKIHPQQISNTLTDEEIAILRESIFEELQLAIEAKGTTVFSYLNADGHAGSFQNQLHV
YHRQGLPCQRCGTPIERIKVAQRGTHFCPHCQVLR
>Mature_274_residues
PELPEVETVRRGLAALVEGKIVTNVVVRYSKMVSPKAEIFAEELEGKKILNVRRRGKYLLIDFSGDYTMVSHLRMEGKYS
VVDRREEYGKHDHVIFELDDGKDLRYNDTRKFGRMNLVPTGEELQVGGLKTIGPEPTPETLTLEYLTHQLRNRKRGMKSF
LLDQSMIAGLGNIYADEVLWLSKIHPQQISNTLTDEEIAILRESIFEELQLAIEAKGTTVFSYLNADGHAGSFQNQLHVY
HRQGLPCQRCGTPIERIKVAQRGTHFCPHCQVLR

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=273, Percent_Identity=38.8278388278388, Blast_Score=190, Evalue=9e-50,
Organism=Escherichia coli, GI1786932, Length=276, Percent_Identity=30.4347826086957, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_PEDPA (Q03GC2)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804192.1
- ProteinModelPortal:   Q03GC2
- SMR:   Q03GC2
- STRING:   Q03GC2
- GeneID:   4418119
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0689
- NMPDR:   fig|278197.10.peg.613
- eggNOG:   COG0266
- HOGENOM:   HBG690070
- OMA:   RSTFYCA
- PhylomeDB:   Q03GC2
- BioCyc:   PPEN278197:PEPE_0689-MONOMER
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 31395; Mature: 31263

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 58-58 ACT_SITE 263-263 BINDING 92-92 BINDING 111-111 BINDING 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVETVRRGLAALVEGKIVTNVVVRYSKMVSPKAEIFAEELEGKKILNVRRRGKYL
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCCCEE
LIDFSGDYTMVSHLRMEGKYSVVDRREEYGKHDHVIFELDDGKDLRYNDTRKFGRMNLVP
EEEECCCCCEEHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHCCCCCCC
TGEELQVGGLKTIGPEPTPETLTLEYLTHQLRNRKRGMKSFLLDQSMIAGLGNIYADEVL
CCCCEEECCEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLSKIHPQQISNTLTDEEIAILRESIFEELQLAIEAKGTTVFSYLNADGHAGSFQNQLHV
HHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
YHRQGLPCQRCGTPIERIKVAQRGTHFCPHCQVLR
HHHCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
PELPEVETVRRGLAALVEGKIVTNVVVRYSKMVSPKAEIFAEELEGKKILNVRRRGKYL
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCCCEE
LIDFSGDYTMVSHLRMEGKYSVVDRREEYGKHDHVIFELDDGKDLRYNDTRKFGRMNLVP
EEEECCCCCEEHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHCCCCCCC
TGEELQVGGLKTIGPEPTPETLTLEYLTHQLRNRKRGMKSFLLDQSMIAGLGNIYADEVL
CCCCEEECCEECCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLSKIHPQQISNTLTDEEIAILRESIFEELQLAIEAKGTTVFSYLNADGHAGSFQNQLHV
HHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
YHRQGLPCQRCGTPIERIKVAQRGTHFCPHCQVLR
HHHCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA