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The map label for this gene is metK

Identifier: 116492411

GI number: 116492411

Start: 681545

End: 682735

Strand: Direct

Name: metK

Synonym: PEPE_0641

Alternate gene names: 116492411

Gene position: 681545-682735 (Clockwise)

Preceding gene: 116492409

Following gene: 116492412

Centisome position: 37.19

GC content: 40.81

Gene sequence:

>1191_bases
ATGTCAGAAAGACATTTATTTACTTCAGAATCAGTATCTGAAGGACATCCAGACAAGATAGCCGATCAAATCAGTGACTC
TATCTTAGATGAACTATTAAAGAACGATCCAGATGCACGAGTTGCTTGTGAAACAACCGTTACGACTGGATTAGTAGTCG
TTGTGGGAGAAATCTCAACAACAGCTTACATCGATATCCAAAAGGTGGTTAGAAAGACCATTAAGGATATTGGCTATGTA
GGTGGAGAATATGGATTTGATGGCGAAAATTGTGCTGTAATTGTTGCTATTGATGAACAATCTCCAGATATTGCCCAAGG
AGTTGATGATTCCTTGGAAACGCGTGAAGGCCATGAAGATCGACTAAACCAAATCGGTGCCGGAGATCAAGGTTTAATGT
TCGGATACGCAACTAATGAAACAGAAGAATTAATGCCATTACCAATTATGTTAAGTCATCATTTGATGCAAAGAATTGCT
AAACTACGTAAGGAACAAGTTATCAGCTATCTTCGTCCAGATGCTAAGGCTGAAGTGACGGTTGAATATGATGATAATGA
TCATCCAGTTCGCGTAGATACGGTTGTTTTAAGTACTCAACATGATCCTGACGTAACTTTAGAACAAATTCGCCAAGATG
TTATTGAACAAGTTATCAAAGCTGTAATCCCAGCTACTCTTTTAGATGAAAACACTAATTTCTACATCAACCCTACAGGT
CGCTTTGTTATTGGTGGACCACAAGGGGATGCCGGTTTGACAGGGCGTAAGATCATCGTAGATACCTACGGTGGGGCCGC
TCGTCATGGTGGTGGAGCTTTCTCTGGAAAAGATGCGACTAAGGTGGACCGTTCAGCAAGTTATGCCGCTCGTTACATTG
CCAAAAACATTGTAGCAGCTAAGTTAGCTGAAAGGGTTGAAGTTCAGATTGCTTATGCAATTGGGGTCGCAGAACCTGTT
TCGGTCATGGTGGATACTTTTGGTACCGGTACGGTTTCGGAAGAAAAATTAACACAAGCCGTTCGTAAGATTTTTGATTT
ACGACCTGCTGGAATTATTGAAATGTTAGATTTGAAACGTCCAATTTACAAGCAGACGGCTGCTTATGGTCATTTCGGAC
GGACTGACGTTGAACTACCATGGGAAAAGACGGATAAGGTTGAAGCTTTAAACGACTTTTTTAAAAACTAA

Upstream 100 bases:

>100_bases
AAGAGTGGGTGTGATAGACTTAGTAAGTTACTAGTGTTCCCGATAGGATTAGTTATCCAAAAGATCCTTGTAACCGAAAT
TAAATTTAGGGGGAATTTAC

Downstream 100 bases:

>100_bases
AAAATTTGATTAATGGCTGTGTTGATGTCTAAACGGATATTAACACGGCTATTATATTGGTTGAGAGGTAATAAATGGAG
AAAAAACAAACAAATGTGGT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSERHLFTSESVSEGHPDKIADQISDSILDELLKNDPDARVACETTVTTGLVVVVGEISTTAYIDIQKVVRKTIKDIGYV
GGEYGFDGENCAVIVAIDEQSPDIAQGVDDSLETREGHEDRLNQIGAGDQGLMFGYATNETEELMPLPIMLSHHLMQRIA
KLRKEQVISYLRPDAKAEVTVEYDDNDHPVRVDTVVLSTQHDPDVTLEQIRQDVIEQVIKAVIPATLLDENTNFYINPTG
RFVIGGPQGDAGLTGRKIIVDTYGGAARHGGGAFSGKDATKVDRSASYAARYIAKNIVAAKLAERVEVQIAYAIGVAEPV
SVMVDTFGTGTVSEEKLTQAVRKIFDLRPAGIIEMLDLKRPIYKQTAAYGHFGRTDVELPWEKTDKVEALNDFFKN

Sequences:

>Translated_396_residues
MSERHLFTSESVSEGHPDKIADQISDSILDELLKNDPDARVACETTVTTGLVVVVGEISTTAYIDIQKVVRKTIKDIGYV
GGEYGFDGENCAVIVAIDEQSPDIAQGVDDSLETREGHEDRLNQIGAGDQGLMFGYATNETEELMPLPIMLSHHLMQRIA
KLRKEQVISYLRPDAKAEVTVEYDDNDHPVRVDTVVLSTQHDPDVTLEQIRQDVIEQVIKAVIPATLLDENTNFYINPTG
RFVIGGPQGDAGLTGRKIIVDTYGGAARHGGGAFSGKDATKVDRSASYAARYIAKNIVAAKLAERVEVQIAYAIGVAEPV
SVMVDTFGTGTVSEEKLTQAVRKIFDLRPAGIIEMLDLKRPIYKQTAAYGHFGRTDVELPWEKTDKVEALNDFFKN
>Mature_395_residues
SERHLFTSESVSEGHPDKIADQISDSILDELLKNDPDARVACETTVTTGLVVVVGEISTTAYIDIQKVVRKTIKDIGYVG
GEYGFDGENCAVIVAIDEQSPDIAQGVDDSLETREGHEDRLNQIGAGDQGLMFGYATNETEELMPLPIMLSHHLMQRIAK
LRKEQVISYLRPDAKAEVTVEYDDNDHPVRVDTVVLSTQHDPDVTLEQIRQDVIEQVIKAVIPATLLDENTNFYINPTGR
FVIGGPQGDAGLTGRKIIVDTYGGAARHGGGAFSGKDATKVDRSASYAARYIAKNIVAAKLAERVEVQIAYAIGVAEPVS
VMVDTFGTGTVSEEKLTQAVRKIFDLRPAGIIEMLDLKRPIYKQTAAYGHFGRTDVELPWEKTDKVEALNDFFKN

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=386, Percent_Identity=59.0673575129534, Blast_Score=443, Evalue=1e-124,
Organism=Homo sapiens, GI5174529, Length=387, Percent_Identity=57.6227390180879, Blast_Score=434, Evalue=1e-121,
Organism=Escherichia coli, GI1789311, Length=389, Percent_Identity=63.2390745501285, Blast_Score=507, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17551082, Length=386, Percent_Identity=56.9948186528497, Blast_Score=437, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17538498, Length=396, Percent_Identity=55.3030303030303, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25145633, Length=394, Percent_Identity=55.0761421319797, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17538494, Length=385, Percent_Identity=55.8441558441558, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32565905, Length=385, Percent_Identity=51.1688311688312, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565911, Length=348, Percent_Identity=53.735632183908, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25145635, Length=347, Percent_Identity=53.314121037464, Blast_Score=357, Evalue=7e-99,
Organism=Caenorhabditis elegans, GI32565903, Length=310, Percent_Identity=55.1612903225806, Blast_Score=329, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI32565909, Length=77, Percent_Identity=58.4415584415584, Blast_Score=101, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6323209, Length=388, Percent_Identity=57.9896907216495, Blast_Score=433, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6320710, Length=390, Percent_Identity=55.3846153846154, Blast_Score=427, Evalue=1e-120,
Organism=Drosophila melanogaster, GI45552159, Length=390, Percent_Identity=54.8717948717949, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580529, Length=390, Percent_Identity=54.8717948717949, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580537, Length=390, Percent_Identity=54.8717948717949, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580533, Length=390, Percent_Identity=54.8717948717949, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580535, Length=390, Percent_Identity=54.8717948717949, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580531, Length=390, Percent_Identity=54.8717948717949, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580527, Length=389, Percent_Identity=54.2416452442159, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580525, Length=389, Percent_Identity=54.2416452442159, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580541, Length=391, Percent_Identity=51.9181585677749, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580539, Length=125, Percent_Identity=52.8, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_PEDPA (Q03GG8)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804146.1
- ProteinModelPortal:   Q03GG8
- SMR:   Q03GG8
- STRING:   Q03GG8
- GeneID:   4418527
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0641
- NMPDR:   fig|278197.10.peg.569
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- PhylomeDB:   Q03GG8
- ProtClustDB:   PRK05250
- BioCyc:   PPEN278197:PEPE_0641-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 43438; Mature: 43307

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERHLFTSESVSEGHPDKIADQISDSILDELLKNDPDARVACETTVTTGLVVVVGEIST
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCEEEEEECCCC
TAYIDIQKVVRKTIKDIGYVGGEYGFDGENCAVIVAIDEQSPDIAQGVDDSLETREGHED
EEEEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCCHHHHCCCHHH
RLNQIGAGDQGLMFGYATNETEELMPLPIMLSHHLMQRIAKLRKEQVISYLRPDAKAEVT
HHHHCCCCCCCEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VEYDDNDHPVRVDTVVLSTQHDPDVTLEQIRQDVIEQVIKAVIPATLLDENTNFYINPTG
EEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHEEECCCCEEECCCC
RFVIGGPQGDAGLTGRKIIVDTYGGAARHGGGAFSGKDATKVDRSASYAARYIAKNIVAA
CEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KLAERVEVQIAYAIGVAEPVSVMVDTFGTGTVSEEKLTQAVRKIFDLRPAGIIEMLDLKR
HHHHHHEEEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCC
PIYKQTAAYGHFGRTDVELPWEKTDKVEALNDFFKN
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SERHLFTSESVSEGHPDKIADQISDSILDELLKNDPDARVACETTVTTGLVVVVGEIST
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCEEEEEECCCC
TAYIDIQKVVRKTIKDIGYVGGEYGFDGENCAVIVAIDEQSPDIAQGVDDSLETREGHED
EEEEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHCCCCHHHHCCCHHH
RLNQIGAGDQGLMFGYATNETEELMPLPIMLSHHLMQRIAKLRKEQVISYLRPDAKAEVT
HHHHCCCCCCCEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
VEYDDNDHPVRVDTVVLSTQHDPDVTLEQIRQDVIEQVIKAVIPATLLDENTNFYINPTG
EEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHEEECCCCEEECCCC
RFVIGGPQGDAGLTGRKIIVDTYGGAARHGGGAFSGKDATKVDRSASYAARYIAKNIVAA
CEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
KLAERVEVQIAYAIGVAEPVSVMVDTFGTGTVSEEKLTQAVRKIFDLRPAGIIEMLDLKR
HHHHHHEEEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCC
PIYKQTAAYGHFGRTDVELPWEKTDKVEALNDFFKN
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA